##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936193.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	306498
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74950570639939	31.0	31.0	34.0	30.0	34.0
2	31.910240849858727	31.0	31.0	34.0	30.0	34.0
3	31.664555723038976	31.0	31.0	34.0	30.0	34.0
4	35.5389366325392	37.0	35.0	37.0	33.0	37.0
5	31.89034186193711	37.0	35.0	37.0	0.0	37.0
6	33.76301966081345	37.0	35.0	37.0	19.0	37.0
7	20.84052098219238	32.0	0.0	37.0	0.0	37.0
8	28.004107694014316	35.0	17.0	37.0	17.0	37.0
9	34.56014394873702	35.0	32.0	39.0	28.0	39.0
10	36.047011726014524	37.0	35.0	39.0	32.0	39.0
11	36.45957885532695	37.0	35.0	39.0	32.0	39.0
12	36.77596591168621	39.0	35.0	39.0	33.0	39.0
13	36.50098532453719	39.0	35.0	39.0	32.0	39.0
14	37.7944358527625	40.0	37.0	41.0	33.0	41.0
15	37.936316060789956	40.0	37.0	41.0	33.0	41.0
16	38.04716507122396	40.0	37.0	41.0	33.0	41.0
17	37.98547135707248	40.0	37.0	41.0	33.0	41.0
18	37.83192059980816	40.0	37.0	41.0	33.0	41.0
19	37.765336152275054	40.0	37.0	41.0	33.0	41.0
20	37.750765094715135	40.0	36.0	41.0	33.0	41.0
21	37.697420537817536	39.0	36.0	41.0	33.0	41.0
22	37.71315636643632	39.0	36.0	41.0	33.0	41.0
23	37.68180216510385	39.0	36.0	41.0	33.0	41.0
24	37.512567781845235	39.0	35.0	41.0	33.0	41.0
25	37.389447891992766	39.0	35.0	41.0	33.0	41.0
26	37.23431148000966	39.0	35.0	41.0	32.0	41.0
27	37.199518430789105	39.0	35.0	41.0	32.0	41.0
28	37.06336746079909	39.0	35.0	41.0	32.0	41.0
29	36.997122330325155	39.0	35.0	41.0	32.0	41.0
30	36.79583227296752	39.0	35.0	40.0	31.0	41.0
31	36.64189978401164	39.0	35.0	40.0	31.0	41.0
32	36.531089925546006	39.0	35.0	40.0	30.0	41.0
33	36.291959490763396	39.0	35.0	40.0	30.0	41.0
34	36.07002982074924	39.0	35.0	40.0	29.0	41.0
35	35.84809688807105	39.0	35.0	40.0	28.0	41.0
36	35.59811157005918	38.0	35.0	40.0	27.0	41.0
37	35.5733707887164	38.0	35.0	40.0	27.0	41.0
38	35.29081755835275	38.0	34.0	40.0	25.0	41.0
39	35.206122062786704	38.0	34.0	40.0	24.0	41.0
40	35.05576219094415	38.0	34.0	40.0	24.0	41.0
41	34.903467559331546	38.0	34.0	40.0	23.0	41.0
42	34.86927157762857	38.0	34.0	40.0	23.0	41.0
43	34.75920560656187	38.0	34.0	40.0	23.0	41.0
44	34.63854902805239	38.0	34.0	40.0	23.0	41.0
45	34.55793186252439	38.0	33.0	40.0	23.0	41.0
46	34.3870172072901	37.0	33.0	40.0	23.0	41.0
47	34.2786021442228	37.0	33.0	40.0	22.0	41.0
48	34.13521132274925	37.0	33.0	40.0	22.0	41.0
49	33.99721368491801	37.0	33.0	40.0	21.0	41.0
50	33.853503122369474	37.0	33.0	40.0	20.0	41.0
51	31.800742582333328	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	10.0
10	15.0
11	11.0
12	12.0
13	7.0
14	9.0
15	18.0
16	39.0
17	83.0
18	184.0
19	293.0
20	508.0
21	831.0
22	1291.0
23	1901.0
24	2999.0
25	3980.0
26	4817.0
27	5472.0
28	5914.0
29	6674.0
30	7902.0
31	9909.0
32	12743.0
33	18082.0
34	22958.0
35	28613.0
36	35326.0
37	53778.0
38	50664.0
39	31442.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.25247799333112	19.257874439637455	23.47649903098878	15.01314853604265
2	32.95747443702732	27.369183485699743	23.85888325535566	15.81445882191727
3	31.018473203740314	26.16721805688781	27.766249698203577	15.048059041168294
4	26.322194598333432	23.68074179929396	32.1411558966127	17.855907705759908
5	23.166872214500582	34.411970061794854	25.0879287956202	17.33322892808436
6	23.992326214200418	35.224699671776	27.84096470450052	12.942009409523065
7	45.852827750915175	42.49097873395585	8.908704135100393	2.747489380028581
8	77.94928515031093	6.702164451317789	11.499911908071178	3.8486384903001
9	72.31270677133293	6.599390534359115	13.086545426071297	8.001357268236662
10	41.17253619925742	24.143387558809522	20.653642111857174	14.03043413007589
11	31.114395526235082	24.26638999275689	27.11078049448936	17.508433986518675
12	27.893167328987467	21.757075086949996	31.26121540760462	19.08854217645792
13	23.718588702046993	22.673883679501987	34.698105697263934	18.90942192118709
14	20.319219048737676	26.24584826002127	32.69776638020476	20.737166311036287
15	19.460485875927414	24.507827131009012	37.39535005122382	18.63633694183975
16	23.609941989833537	23.944038786549992	31.31407056489765	21.131948658718816
17	23.942407454534774	23.19232099393797	31.14375950250899	21.721512049018266
18	23.937513458489125	24.41810387017207	31.780957787652774	19.863424883686026
19	24.39102375871947	25.57928599860358	28.393659991256058	21.63603025142089
20	24.461171035373802	26.14046421183825	30.648160836286042	18.7502039165019
21	24.138167296360823	26.65955405908032	31.079485021109438	18.122793623449418
22	23.982211955706074	22.675515011517202	31.21292797995419	22.12934505282253
23	22.307160242481192	25.960038890955246	31.681446534724532	20.051354331839033
24	23.183838067458844	23.67193260641179	31.330710151452863	21.813519174676507
25	23.098682536264512	26.87913134832854	29.460551129208024	20.561634986198932
26	21.680728748637836	24.114349848938655	30.085351291036154	24.11957011138735
27	22.19557713264034	25.0729205410802	30.37507585693871	22.356426469340747
28	19.77663802047648	25.81321900958571	31.869049716474496	22.541093253463316
29	23.93425079445869	25.126754497582365	29.411284902348466	21.527709805610478
30	22.070290833871674	25.20244830308844	31.30656643762765	21.420694425412236
31	23.723156431689603	26.156124999184332	28.798556597432935	21.322161971693127
32	25.485647540930117	24.927079458919795	27.961030740820497	21.626242259329587
33	23.159368087230586	25.447800638177082	28.212582137566965	23.180249137025363
34	22.89704989918368	24.151544218885604	32.80934949004561	20.142056391885102
35	22.37600245352335	25.319577941781024	29.524173077801485	22.78024652689414
36	23.952847979432164	28.291864873506515	28.669681368230787	19.08560577883053
37	22.139133044913834	26.674562313620314	29.20736840044633	21.978936241019518
38	22.332609021918575	29.055980789434187	27.38973826909148	21.221671919555757
39	22.711078049448936	24.87291923601459	29.489915105481927	22.926087609054548
40	24.851059387010682	25.00701472766543	29.684369881695805	20.45755600362808
41	20.492140242350683	25.348289385248847	30.878504916834693	23.281065455565777
42	22.764259473145014	24.843555259740683	30.754523683678197	21.637661583436106
43	22.582855353052874	24.3747104385673	29.668709094349722	23.37372511403011
44	22.443213332550293	24.42267159981468	29.892201580434456	23.24191348720057
45	21.325098369320518	24.64355395467507	28.929715691456387	25.10163198454802
46	23.234083093527527	26.38516401412081	28.375389072685632	22.005363819666034
47	20.219707795809434	25.355140979712754	32.730719286912155	21.69443193756566
48	22.011562881323858	26.26672930981605	29.4070434391089	22.31466436975119
49	21.60666627514698	24.93001585654719	31.316680696121995	22.14663717218383
50	20.450378142761128	24.98450234585544	31.18128013885898	23.383839372524452
51	21.179583553563155	24.565576284347696	29.2709903490398	24.98384981304935
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	391.0
1	651.0
2	911.0
3	3536.5
4	6162.0
5	4115.0
6	2068.0
7	1803.0
8	1538.0
9	1520.5
10	1503.0
11	1466.0
12	1429.0
13	1397.0
14	1365.0
15	1327.0
16	1289.0
17	1314.5
18	1340.0
19	1289.5
20	1239.0
21	1347.5
22	1456.0
23	1702.5
24	1949.0
25	2279.0
26	3068.0
27	3527.0
28	4078.5
29	4630.0
30	5178.0
31	5726.0
32	6720.5
33	7715.0
34	8575.5
35	9436.0
36	10106.5
37	10777.0
38	11335.5
39	11894.0
40	12703.5
41	13513.0
42	14487.0
43	15461.0
44	17946.5
45	20432.0
46	24172.5
47	27913.0
48	24424.5
49	20936.0
50	20701.0
51	20466.0
52	19113.0
53	17760.0
54	17038.0
55	16316.0
56	15855.5
57	15395.0
58	14757.0
59	14119.0
60	13111.0
61	12103.0
62	11129.0
63	10155.0
64	8940.5
65	7726.0
66	6477.5
67	5229.0
68	4431.0
69	3633.0
70	3081.0
71	2529.0
72	2141.5
73	1754.0
74	1374.0
75	759.5
76	525.0
77	398.0
78	271.0
79	224.5
80	178.0
81	127.0
82	76.0
83	50.5
84	25.0
85	19.0
86	13.0
87	11.0
88	9.0
89	6.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	2.0
96	3.0
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	306498.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.17195030328304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.99973530411718	34.62884051221081
2	8.179827552337263	7.553571824718827
3	4.172882334086184	5.780103472526251
4	2.7647688265943815	5.106190754463277
5	1.9338697017149604	4.464526788030397
6	1.5279874164466718	4.233009543373054
7	1.2633645862090621	4.0832404822560795
8	0.9940075960826762	3.671621546193213
9	0.8066278794127404	3.3519224125338862
>10	3.3176376711643654	21.522574148148603
>50	0.01714522116414541	0.6029568881418023
>100	0.016430836948972686	1.5984294748004235
>500	0.001428768430345451	0.5455638364258977
>1k	0.004286305291036353	2.85744831617748
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2168	0.7073455617981195	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG	1608	0.5246363760938081	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	1587	0.5177847816298964	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1233	0.40228647495252823	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGC	1066	0.3477999856442783	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG	1001	0.32659266944645643	No Hit
GAATCTATCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	976	0.3184360093703711	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGC	678	0.22120862126343402	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	463	0.15106134460910023	No Hit
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	369	0.1203923027230194	No Hit
GAATATGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	338	0.11027804422867359	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCT	317	0.10342644976476192	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG	314	0.1024476505556317	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050571292471729014	0.0
2	0.0	0.0	0.0	0.34225345679254027	0.0
3	0.0	0.0	0.0	0.42186245913513304	0.0
4	0.0	0.0	0.0	0.6329568219042212	0.0
5	0.0	0.0	0.0	1.481249469817095	0.0
6	0.0	0.0	0.0	1.7194239440387866	0.0
7	0.0	0.0	0.0	2.053194474352198	0.0
8	0.0	0.0	0.0	2.619592950035563	0.0
9	0.0	0.0	0.0	2.8026284021429175	0.0
10	0.0	0.0	0.0	4.261039223746974	0.0
11	0.0	0.0	0.0	4.6610418338781985	0.0
12	0.0	0.0	0.0	5.724996574202768	0.0
13	0.0	0.0	0.0	5.916514952789251	0.0
14	0.0	0.0	0.0	6.036254722706184	0.0
15	0.0	0.0	0.0	6.233645896547449	0.0
16	0.0	0.0	0.0	6.4969428838034835	0.0
17	0.0	0.0	0.0	6.7654601335082125	0.0
18	0.0	0.0	0.0	7.081612278057279	0.0
19	0.0	0.0	0.0	7.267584127792024	0.0
20	0.0	0.0	0.0	7.474437027321549	0.0
21	0.0	0.0	0.0	7.7064124398854155	0.0
22	0.0	0.0	0.0	7.979171152829709	0.0
23	0.0	0.0	0.0	8.23561654562183	0.0
24	0.0	0.0	0.0	8.44018558033005	0.0
25	0.0	0.0	0.0	8.642470750216967	0.0
26	0.0	0.0	0.0	8.832684063191277	0.0
27	0.0	0.0	0.0	9.035948032287322	0.0
28	0.0	0.0	0.0	9.226161345261634	0.0
29	0.0	0.0	0.0	9.447043700122023	0.0
30	0.0	0.0	0.0	9.701205228092842	0.0
31	0.0	0.0	0.0	9.926981578998884	0.0
32	0.0	0.0	0.0	10.162872188399271	0.0
33	0.0	0.0	0.0	10.396152666575311	0.0
34	0.0	0.0	0.0	10.636611005618308	0.0
35	0.0	0.0	0.0	10.895340263231734	0.0
36	0.0	0.0	0.0	11.135146069468643	0.0
37	0.0	0.0	0.0	11.393222794275982	0.0
38	0.0	0.0	0.0	11.671528036072013	0.0
39	0.0	0.0	0.0	12.136131394005833	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACG	20	7.0251804E-4	45.000004	26
TATGCGG	30	2.1602737E-6	45.000004	2
GGCCCGA	35	1.2080272E-7	45.000004	24
CCCAATG	20	7.0251804E-4	45.000004	24
ATATAGG	30	2.1602737E-6	45.000004	3
CCGGTAA	20	7.0251804E-4	45.000004	41
ACCCGCT	20	7.0251804E-4	45.000004	34
AGTACGG	20	7.0251804E-4	45.000004	2
ATCGATG	20	7.0251804E-4	45.000004	32
TCACGGG	25	3.884089E-5	45.0	3
CGTTTTA	445	0.0	43.988766	1
GTAAGGC	50	1.0768417E-9	40.5	4
GAAACGG	130	0.0	39.80769	5
TATCTCT	210	0.0	39.642857	6
ATAGGAT	40	3.4471668E-7	39.375004	5
AGGCGAT	120	0.0	39.375004	7
CGTTATT	195	0.0	39.23077	1
TACGGCT	190	0.0	39.078945	7
ACACGCG	260	0.0	38.942307	36
CTATCTC	215	0.0	38.720932	5
>>END_MODULE