SRR2936192.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936192.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82933
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1304	1.5723535866301714	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	775	0.9344892865325022	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	599	0.7222697840425404	No Hit
GAATCTATCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	418	0.5040213184136593	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	402	0.4847286363691173	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	356	0.42926217549105905	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	309	0.372589921985217	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	284	0.34244510629062014	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	269	0.324358216873862	No Hit
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	251	0.3026539495737523	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	207	0.24959907395126188	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	192	0.23151218453450378	TruSeq Adapter, Index 16 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	179	0.2158368803733134	No Hit
CCTGTCACTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	178	0.21463108774552953	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCACTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	154	0.18569206467871655	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	147	0.17725151628422944	No Hit
CTGTCTATTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	133	0.1603704194952552	TruSeq Adapter, Index 16 (95% over 22bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	133	0.1603704194952552	No Hit
GAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT	127	0.15313566372855197	No Hit
GAATATGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	119	0.14348932270628098	No Hit
CGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG	114	0.1374603595673616	No Hit
GAATGAATGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	103	0.124196640661739	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	99	0.1193734701506035	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	98	0.11816767752281963	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95	0.114550299639468	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT	95	0.114550299639468	No Hit
GAATCTATCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTT	91	0.1097271291283325	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT	90	0.10852133650054864	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC	88	0.1061097512449809	No Hit
GAATGAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC	83	0.10008078810606151	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTCA	25	3.8594633E-5	45.000004	1
GTATGGG	20	6.995433E-4	45.000004	3
GGGTCCA	20	6.995433E-4	45.000004	7
TAGGACG	20	6.995433E-4	45.000004	11
ATCTTAT	20	6.995433E-4	45.000004	7
TAGGAAC	20	6.995433E-4	45.000004	6
TTACTGG	25	3.8594633E-5	45.000004	40
ATCTATC	50	2.1827873E-11	45.000004	3
GCAGGGC	25	3.8594633E-5	45.000004	4
AGGGCGA	25	3.8594633E-5	45.000004	6
GGCGATG	20	6.995433E-4	45.000004	8
AGGAAAC	20	6.995433E-4	45.000004	7
TATTGGG	20	6.995433E-4	45.000004	3
GAATGCT	20	6.995433E-4	45.000004	1
AGGGATT	50	2.1827873E-11	45.000004	6
TATCTTA	20	6.995433E-4	45.000004	6
AGAGCGG	25	3.8594633E-5	45.000004	2
ACTTACT	25	3.8594633E-5	45.000004	38
CTTACTG	25	3.8594633E-5	45.000004	39
GAATCTA	50	2.1827873E-11	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content