##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936192.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82933
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25725585713769	31.0	31.0	33.0	30.0	34.0
2	31.433361870425525	31.0	31.0	34.0	30.0	34.0
3	30.813729154859946	31.0	30.0	34.0	27.0	34.0
4	34.98413176901836	35.0	35.0	37.0	32.0	37.0
5	31.552687108871016	35.0	35.0	37.0	0.0	37.0
6	33.49073348365548	35.0	35.0	37.0	18.0	37.0
7	21.05584025659267	32.0	0.0	37.0	0.0	37.0
8	28.080655468872465	35.0	17.0	37.0	17.0	37.0
9	34.280503539001366	35.0	32.0	39.0	28.0	39.0
10	35.7279490673194	37.0	34.0	39.0	32.0	39.0
11	36.31690641843416	37.0	35.0	39.0	32.0	39.0
12	36.82712551095462	39.0	35.0	39.0	33.0	39.0
13	36.668117637128766	39.0	35.0	39.0	32.0	39.0
14	38.00607719484403	40.0	37.0	40.0	33.0	41.0
15	38.06875429563624	40.0	37.0	41.0	33.0	41.0
16	38.11112584857656	40.0	37.0	41.0	33.0	41.0
17	38.04969071419097	40.0	37.0	41.0	33.0	41.0
18	37.86856860357156	39.0	37.0	40.0	33.0	41.0
19	37.745216017749264	39.0	37.0	40.0	33.0	41.0
20	37.61939155704002	39.0	36.0	40.0	33.0	41.0
21	37.54581409089265	39.0	36.0	40.0	33.0	41.0
22	37.46729287497136	39.0	35.0	40.0	33.0	41.0
23	37.41614315170077	39.0	35.0	40.0	33.0	41.0
24	37.19531428984843	39.0	35.0	40.0	33.0	41.0
25	36.9356347895289	38.0	35.0	40.0	32.0	41.0
26	36.64678716554327	38.0	35.0	40.0	31.0	41.0
27	36.61956036800791	38.0	35.0	40.0	31.0	41.0
28	36.33284699697346	38.0	35.0	40.0	31.0	41.0
29	36.1055550866362	38.0	35.0	40.0	30.0	41.0
30	36.01389073107207	38.0	34.0	40.0	30.0	41.0
31	35.665006692149085	38.0	34.0	40.0	29.0	41.0
32	35.55543631606236	38.0	34.0	40.0	29.0	41.0
33	35.379185607659196	38.0	34.0	40.0	27.0	41.0
34	34.75405447771092	38.0	33.0	40.0	25.0	41.0
35	34.48718845332979	38.0	33.0	40.0	23.0	41.0
36	33.98051439113501	37.0	33.0	40.0	21.0	41.0
37	33.84745517465906	37.0	33.0	40.0	19.0	41.0
38	33.757780376930775	37.0	33.0	40.0	18.0	41.0
39	33.604222685782496	37.0	33.0	40.0	16.0	41.0
40	33.42047194723452	37.0	33.0	40.0	15.0	41.0
41	33.18840509809123	37.0	32.0	40.0	15.0	41.0
42	32.95542184655083	37.0	31.0	40.0	15.0	41.0
43	32.774589126162084	37.0	31.0	40.0	15.0	41.0
44	32.49146901715843	37.0	30.0	40.0	13.0	41.0
45	32.3932210338466	37.0	30.0	40.0	12.0	41.0
46	31.968396175225784	36.0	30.0	40.0	10.0	41.0
47	31.77409475118469	36.0	30.0	40.0	10.0	41.0
48	31.549419410849723	35.0	28.0	40.0	10.0	41.0
49	31.282746313289042	35.0	27.0	40.0	8.0	41.0
50	30.99615352151737	35.0	27.0	39.0	8.0	41.0
51	28.424873090325924	33.0	21.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	2.0
12	1.0
13	0.0
14	2.0
15	2.0
16	7.0
17	9.0
18	18.0
19	55.0
20	124.0
21	229.0
22	445.0
23	685.0
24	1168.0
25	1789.0
26	2277.0
27	2403.0
28	2525.0
29	2572.0
30	2891.0
31	3280.0
32	4247.0
33	5386.0
34	6589.0
35	8613.0
36	10088.0
37	12259.0
38	10138.0
39	5122.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.07088854858742	19.571220141560055	21.88272460902174	21.47516670083079
2	36.75135350222469	28.111849324153233	21.4522566409029	13.684540532719183
3	25.699058275957704	26.031857041226047	33.95873777627724	14.310346906539012
4	23.588921177335923	21.566806940542367	38.967600352091445	15.876671530030265
5	20.012540243328953	31.99209000036174	30.569254699576764	17.426115056732545
6	18.353369587498342	32.25615858584641	35.49371179144611	13.896760035209144
7	37.68343120350162	41.72404229920538	15.922491649886053	4.670034847406943
8	60.63689966599545	7.936527076073458	23.550335813246836	7.8762374446842625
9	55.014288642639244	7.83765208059518	25.475986639817684	11.672072636947897
10	28.137170969336694	22.228787093195713	33.809219490432035	15.824822447035558
11	19.38311649162577	23.56962849529138	36.618716313168456	20.42853869991439
12	17.986808628652046	20.585291741526294	41.648077363655	19.779822266166665
13	16.27337730457116	22.469945618752487	44.95797812692173	16.298698949754623
14	14.428514584061833	27.019401203381044	38.896458587052194	19.655625625504925
15	12.857366790059446	25.392786948500596	45.11834854641699	16.63149771502297
16	13.856968878492276	27.623503309900766	37.18785043348245	21.33167737812451
17	13.706244800019293	26.480411898761652	37.33013396356095	22.48320933765811
18	14.199413984782897	27.584917945811675	38.900075964935546	19.31559210446987
19	14.549093846840218	28.240869135326108	35.369515150784366	21.840521867049308
20	14.371842330555992	28.367477361243417	38.54195555448374	18.718724753716856
21	15.373856004244391	30.332919344531128	37.4157452401336	16.87747941109088
22	12.769343928231223	25.71111620223554	35.76742671795305	25.75211315158019
23	13.09852531561622	27.625914895156328	38.05481533285906	21.220744456368394
24	17.313976342348646	24.038681827499307	35.828922141970025	22.818419688182026
25	14.242822519383116	30.58251841848239	33.65367224144792	21.520986820686577
26	13.390327131539918	27.75372891370142	36.80923154835831	22.04671240640035
27	17.059554097886245	27.130334125137157	35.18985204924457	20.620259727732023
28	12.55712442574126	24.85138605862564	38.20192203344869	24.38956748218441
29	15.126668515548698	26.151230511376657	36.65247850674641	22.06962246632824
30	14.28743684661112	26.4466497051837	39.61511099321139	19.65080245499379
31	14.435749339828535	25.85098814705847	33.30640396464616	26.406858548466833
32	13.785827113453028	26.697454571762748	36.28109437738898	23.235623937395246
33	11.95905128236046	22.473562996635838	38.80964151785176	26.75774420315194
34	12.133891213389122	23.470753499813103	36.48969650199559	27.90565878480219
35	12.118215909227931	22.269784042540365	36.877961728142	28.73403832008971
36	13.52175852796836	24.418506505251226	34.60383683214161	27.455898134638808
37	12.30149638865108	23.321235213967903	38.94348449953577	25.433783897845245
38	12.159212858572582	24.90202934899256	34.61107158790831	28.327686204526547
39	16.585677595167184	21.713913641132	36.027877925554364	25.672530838146457
40	16.293875779243486	22.403627024224374	36.02546634029879	25.277030856233345
41	13.583253951985338	25.61103541412948	30.77665103155559	30.02905960232959
42	14.938564865614412	23.969951647715625	34.46637647257425	26.62510701409572
43	18.098947343035945	22.639962379270013	33.87071491444901	25.390375363245028
44	16.436159309321983	22.98723065607177	33.9237697900715	26.652840244534744
45	16.179325479604017	21.88875357216066	33.543945112319584	28.38797583591574
46	19.28665308140306	23.566011117408028	32.10302292211785	25.044312879071057
47	13.641131998118963	22.42171391364113	37.917354973291694	26.01979911494821
48	14.933741695103276	22.4988846418193	34.50496183666333	28.062411826414092
49	15.17731180591562	20.256110354141292	38.36952720871064	26.19705063123244
50	15.378679174755524	19.303534178192034	37.39283518020571	27.924951466846732
51	15.004883460142523	19.094932053585424	33.294346038368325	32.60583844790373
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	220.0
1	253.0
2	286.0
3	1884.0
4	3482.0
5	2405.5
6	1329.0
7	1109.0
8	889.0
9	872.5
10	856.0
11	863.0
12	870.0
13	853.5
14	837.0
15	810.0
16	783.0
17	772.5
18	762.0
19	802.0
20	842.0
21	867.5
22	893.0
23	871.0
24	849.0
25	935.5
26	1042.5
27	1063.0
28	1178.5
29	1294.0
30	1426.5
31	1559.0
32	1608.5
33	1658.0
34	1884.5
35	2111.0
36	2172.5
37	2234.0
38	2474.0
39	2714.0
40	3084.0
41	3454.0
42	4044.5
43	4635.0
44	6126.0
45	7617.0
46	9907.0
47	12197.0
48	11091.5
49	9986.0
50	8824.5
51	7663.0
52	5749.0
53	3835.0
54	3008.5
55	2182.0
56	1762.0
57	1342.0
58	1133.5
59	925.0
60	839.5
61	754.0
62	697.0
63	640.0
64	536.0
65	432.0
66	330.0
67	228.0
68	211.0
69	194.0
70	157.5
71	121.0
72	101.0
73	81.0
74	64.0
75	37.0
76	27.0
77	17.0
78	7.0
79	4.5
80	2.0
81	5.0
82	8.0
83	5.0
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	82933.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.96249984927591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.69125504119127	60.65136917752885
2	7.707421322946469	10.784609262898966
3	2.6438247561269863	5.5490576730613865
4	1.1840336424114992	3.3135181411500847
5	0.6187308262383234	2.164397766872053
6	0.3309089655647858	1.3890731072070224
7	0.20854158767364103	1.021306355732941
8	0.12926131467374444	0.7234755766703242
9	0.10340905173899556	0.6511280190032918
>10	0.303333218434387	3.4690653901342046
>50	0.04136362069559822	2.140281914316376
>100	0.03274619971734859	4.913604958219286
>500	0.003446968391299852	1.6567590705750423
>1k	0.001723484195649926	1.5723535866301714
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1304	1.5723535866301714	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	775	0.9344892865325022	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	599	0.7222697840425404	No Hit
GAATCTATCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	418	0.5040213184136593	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	402	0.4847286363691173	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	356	0.42926217549105905	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	309	0.372589921985217	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	284	0.34244510629062014	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	269	0.324358216873862	No Hit
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	251	0.3026539495737523	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	207	0.24959907395126188	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	192	0.23151218453450378	TruSeq Adapter, Index 16 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	179	0.2158368803733134	No Hit
CCTGTCACTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	178	0.21463108774552953	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCACTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	154	0.18569206467871655	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	147	0.17725151628422944	No Hit
CTGTCTATTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	133	0.1603704194952552	TruSeq Adapter, Index 16 (95% over 22bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	133	0.1603704194952552	No Hit
GAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT	127	0.15313566372855197	No Hit
GAATATGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	119	0.14348932270628098	No Hit
CGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTG	114	0.1374603595673616	No Hit
GAATGAATGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	103	0.124196640661739	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	99	0.1193734701506035	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	98	0.11816767752281963	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95	0.114550299639468	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT	95	0.114550299639468	No Hit
GAATCTATCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTT	91	0.1097271291283325	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT	90	0.10852133650054864	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC	88	0.1061097512449809	No Hit
GAATGAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC	83	0.10008078810606151	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.41599845658543644	0.0
2	0.0	0.0	0.0	1.5590898677245488	0.0
3	0.0	0.0	0.0	2.084815453438318	0.0
4	0.0	0.0	0.0	2.797438896458587	0.0
5	0.0	0.0	0.0	5.082415926109028	0.0
6	0.0	0.0	0.0	6.531778664705244	0.0
7	0.0	0.0	0.0	8.011286218996057	0.0
8	0.0	0.0	0.0	10.800284567060157	0.0
9	0.0	0.0	0.0	11.801092448120773	0.0
10	0.0	0.0	0.0	15.054320957881664	0.0
11	0.0	0.0	0.0	17.8107629049956	0.0
12	0.0	0.0	0.0	20.85177191226653	0.0
13	0.0	0.0	0.0	21.95145478880542	0.0
14	0.0	0.0	0.0	22.387951720063185	0.0
15	0.0	0.0	0.0	23.06560717687772	0.0
16	0.0	0.0	0.0	24.42574126101793	0.0
17	0.0	0.0	0.0	26.002918018159235	0.0
18	0.0	0.0	0.0	27.660882881362063	0.0
19	0.0	0.0	0.0	28.605018508916835	0.0
20	0.0	0.0	0.0	29.516597735521444	0.0
21	0.0	0.0	0.0	30.680187621332884	0.0
22	0.0	0.0	0.0	31.717169281227015	0.0
23	0.0	0.0	0.0	32.82046953564926	0.0
24	0.0	0.0	0.0	33.53671035655288	0.0
25	0.0	0.0	0.0	34.31685818672905	0.0
26	0.0	0.0	0.0	34.99210205828802	0.0
27	0.0	0.0	0.0	35.64443586991909	0.0
28	0.0	0.0	0.0	36.3317376677559	0.0
29	0.0	0.0	0.0	37.0347147697539	0.0
30	0.0	0.0	0.0	37.71357601919622	0.0
31	0.0	0.0	0.0	38.33938239301605	0.0
32	0.0	0.0	0.0	38.98448144888042	0.0
33	0.0	0.0	0.0	39.59220093328349	0.0
34	0.0	0.0	0.0	40.155306090458566	0.0
35	0.0	0.0	0.0	40.73167496653925	0.0
36	0.0	0.0	0.0	41.22122677341951	0.0
37	0.0	0.0	0.0	41.691485898255216	0.0
38	0.0	0.0	0.0	42.2087709355745	0.0
39	0.0	0.0	0.0	42.682647438293564	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTCA	25	3.8594633E-5	45.000004	1
GTATGGG	20	6.995433E-4	45.000004	3
GGGTCCA	20	6.995433E-4	45.000004	7
TAGGACG	20	6.995433E-4	45.000004	11
ATCTTAT	20	6.995433E-4	45.000004	7
TAGGAAC	20	6.995433E-4	45.000004	6
TTACTGG	25	3.8594633E-5	45.000004	40
ATCTATC	50	2.1827873E-11	45.000004	3
GCAGGGC	25	3.8594633E-5	45.000004	4
AGGGCGA	25	3.8594633E-5	45.000004	6
GGCGATG	20	6.995433E-4	45.000004	8
AGGAAAC	20	6.995433E-4	45.000004	7
TATTGGG	20	6.995433E-4	45.000004	3
GAATGCT	20	6.995433E-4	45.000004	1
AGGGATT	50	2.1827873E-11	45.000004	6
TATCTTA	20	6.995433E-4	45.000004	6
AGAGCGG	25	3.8594633E-5	45.000004	2
ACTTACT	25	3.8594633E-5	45.000004	38
CTTACTG	25	3.8594633E-5	45.000004	39
GAATCTA	50	2.1827873E-11	45.000004	1
>>END_MODULE