##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936191.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94335 Sequences flagged as poor quality 0 Sequence length 51 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52276461546616 31.0 31.0 34.0 30.0 34.0 2 31.660497164361054 31.0 31.0 34.0 30.0 34.0 3 30.27167011183548 31.0 30.0 34.0 25.0 34.0 4 34.79940637091217 35.0 35.0 37.0 32.0 37.0 5 31.249843642338476 35.0 35.0 37.0 0.0 37.0 6 33.49852122754015 36.0 35.0 37.0 17.0 37.0 7 21.756887687496686 32.0 0.0 37.0 0.0 37.0 8 28.62864260348757 35.0 17.0 37.0 17.0 37.0 9 35.05978692955955 37.0 32.0 39.0 32.0 39.0 10 36.52346424974824 37.0 35.0 39.0 33.0 39.0 11 36.91735835055918 39.0 37.0 39.0 34.0 39.0 12 37.31397678486246 39.0 37.0 39.0 34.0 39.0 13 37.19098955848837 39.0 37.0 39.0 34.0 39.0 14 38.68525997773891 40.0 38.0 41.0 35.0 41.0 15 38.76362961785127 40.0 38.0 41.0 35.0 41.0 16 38.78006042296072 40.0 38.0 41.0 35.0 41.0 17 38.75483118672815 40.0 38.0 41.0 35.0 41.0 18 38.44063179095776 40.0 38.0 41.0 35.0 41.0 19 38.12278581650501 40.0 37.0 41.0 34.0 41.0 20 37.79313086341231 39.0 35.0 41.0 34.0 41.0 21 37.66581862511263 39.0 35.0 41.0 33.0 41.0 22 37.473896220914824 39.0 35.0 40.0 33.0 41.0 23 37.28541898553029 38.0 35.0 40.0 33.0 41.0 24 36.99137117718768 38.0 35.0 40.0 33.0 41.0 25 36.82893941803148 38.0 35.0 40.0 33.0 41.0 26 36.68793130863412 38.0 35.0 40.0 33.0 41.0 27 36.60024381194678 38.0 35.0 40.0 33.0 41.0 28 36.495733290931256 38.0 35.0 40.0 33.0 41.0 29 36.375491599088356 38.0 35.0 40.0 32.0 41.0 30 36.10927015423756 38.0 35.0 40.0 31.0 41.0 31 35.81570996978852 38.0 34.0 40.0 30.0 41.0 32 35.361117294747444 38.0 34.0 40.0 27.0 41.0 33 34.612296602533526 37.0 33.0 40.0 23.0 41.0 34 33.65321460751577 37.0 33.0 40.0 18.0 41.0 35 32.68510096994753 37.0 31.0 40.0 12.0 41.0 36 32.012201197858694 37.0 30.0 40.0 9.0 41.0 37 31.587671595908198 37.0 28.0 40.0 8.0 41.0 38 31.256511369057083 37.0 25.0 40.0 8.0 41.0 39 31.038236073567603 36.0 25.0 40.0 7.0 41.0 40 30.893167965230298 36.0 24.0 40.0 7.0 41.0 41 30.757332909312556 36.0 24.0 40.0 7.0 41.0 42 30.58158689775799 36.0 23.0 40.0 7.0 41.0 43 30.426511899083057 36.0 23.0 40.0 7.0 41.0 44 30.21197858695076 35.0 22.0 40.0 7.0 41.0 45 30.094588434833305 35.0 22.0 40.0 7.0 41.0 46 29.943955053797637 35.0 22.0 40.0 7.0 41.0 47 29.900863942333174 35.0 22.0 40.0 7.0 41.0 48 29.776170032331585 35.0 20.0 40.0 7.0 41.0 49 29.636677797212062 35.0 20.0 40.0 7.0 41.0 50 29.476249536227275 35.0 20.0 40.0 7.0 41.0 51 28.023533153124504 34.0 18.0 38.0 7.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 5.0 10 1.0 11 3.0 12 0.0 13 1.0 14 1.0 15 6.0 16 5.0 17 25.0 18 43.0 19 110.0 20 226.0 21 415.0 22 760.0 23 1373.0 24 2349.0 25 3662.0 26 4553.0 27 4507.0 28 3823.0 29 3171.0 30 2824.0 31 2863.0 32 3238.0 33 4366.0 34 5184.0 35 6950.0 36 8120.0 37 13111.0 38 13604.0 39 9031.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.095934700800342 15.839296125510149 18.884825356442466 35.17994381724704 2 49.124927121428954 19.84099220861822 19.279164679069275 11.754915990883553 3 24.532782106323207 19.937456935389832 44.01759686224625 11.512164096040706 4 19.824031377537498 18.94630836911009 47.85604494620237 13.37361530715005 5 17.19828271585308 31.328775109980388 38.94630836911009 12.526633805056447 6 17.936078867864524 26.56490168018233 45.06492818148089 10.434091270472253 7 35.81173477500397 39.384109821381244 22.52928393491281 2.274871468701966 8 60.716595113160544 4.8073355594424125 31.697673185986115 2.778396141410929 9 56.18275295489479 6.018974929771559 32.896591933004714 4.901680182328934 10 27.061005989293474 24.955742831398737 38.37600042402078 9.607250755287009 11 18.797901097153762 20.040281973816718 48.134838607091744 13.026978321937774 12 17.609582869560608 18.90072613558064 49.327397042455075 14.162293952403667 13 16.650235861557217 18.99189060263953 50.950336566491764 13.407536969311495 14 15.154502570625962 21.32506492818148 49.08676525149732 14.433667249695233 15 13.94392325223936 21.779827211533366 50.75316690517835 13.523082631048922 16 17.258705676578153 21.344145863147293 48.032013568664865 13.36513489160969 17 17.30110775427996 20.943446228865213 47.56453066200243 14.190915354852388 18 17.29050723485451 21.29856362961785 48.03837388032014 13.372555255207505 19 16.20077383791806 22.553665129591348 46.62956484867758 14.615996183813007 20 16.551651030900512 22.70313245349022 47.48502676631155 13.260189749297716 21 17.075316690517838 22.401017649864844 47.745799544177665 12.777866115439657 22 16.741400328616102 19.989399480574548 47.84968463454709 15.419515556262256 23 15.003975194784545 21.942015158742777 48.56839985159273 14.48560979487995 24 15.461917633964065 21.612339004611226 47.77972120633911 15.1460221550856 25 15.593364074839666 23.715482058620875 46.30730905814385 14.383844808395613 26 15.300789738697196 22.565325700959345 46.56914188795251 15.564742672390947 27 15.738591190968357 21.550855991943603 47.58679175279589 15.123761064292152 28 14.380664652567976 22.215508559919435 48.01929294535432 15.384533842158266 29 16.84952562675571 21.464991784597444 46.48327768060635 15.202204908040496 30 16.08840833200827 21.53707531669052 48.11363756824084 14.26087878306037 31 17.323368845073407 21.14697620183389 46.61896432925213 14.91069062384057 32 17.28838713096942 21.685482588646842 46.08575820215191 14.940372078231833 33 16.401123655059095 22.217628663804526 45.26633805056448 16.114909630571898 34 16.251656331160227 23.15895478878465 45.28859914135793 15.300789738697196 35 15.898659034292681 24.756453066200244 43.41972756665077 15.925160332856311 36 17.612763025388244 25.28117877776011 40.978427942969205 16.12763025388244 37 16.44882599247363 26.206604123602055 40.62437059415912 16.720199289765198 38 17.341389728096676 27.725658557269306 38.72687761700323 16.206074097630786 39 17.886256426564902 26.07197752689882 38.28377590501935 17.757990141516935 40 17.745269518206392 26.687867705517572 38.97174961573117 16.595113160544866 41 16.938569989929505 26.252186357131503 38.19897174961573 18.610271903323262 42 17.682726453596224 26.32426988922457 38.355859437112414 17.637144220066784 43 17.87141569936927 25.606614724121478 37.80993268670165 18.7120368898076 44 18.61981237080617 26.02957544919701 37.46647585731701 17.88413632267981 45 18.136428685005566 25.92251020299995 36.71595908199502 19.22510202999947 46 18.74065829225632 26.517199342767796 36.492288122117984 18.2498542428579 47 17.25022526103779 25.87904807335559 38.38130068373351 18.48942598187311 48 17.637144220066784 26.651825939471035 36.807123549053905 18.90390629140828 49 17.73890920655112 25.526050776488045 38.095086659246306 18.63995335771453 50 16.98203211957386 25.60449462023639 37.94561933534743 19.46785392484232 51 17.071076482747653 25.257857635024116 37.34562993586686 20.325435946361374 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 150.0 1 213.0 2 276.0 3 4617.5 4 8959.0 5 6096.0 6 3233.0 7 2801.0 8 2369.0 9 2235.0 10 2101.0 11 2029.0 12 1957.0 13 1794.5 14 1632.0 15 1623.0 16 1614.0 17 1451.5 18 1289.0 19 1243.5 20 1198.0 21 1156.0 22 1114.0 23 1110.0 24 1106.0 25 1173.5 26 1345.0 27 1449.0 28 1628.0 29 1807.0 30 1978.5 31 2150.0 32 2450.5 33 2751.0 34 3033.5 35 3316.0 36 3507.5 37 3699.0 38 3820.5 39 3942.0 40 4085.5 41 4229.0 42 4442.0 43 4655.0 44 4999.0 45 5343.0 46 5712.0 47 6081.0 48 5782.5 49 5484.0 50 5189.0 51 4894.0 52 4342.0 53 3790.0 54 3500.5 55 3211.0 56 2859.5 57 2508.0 58 2274.0 59 2040.0 60 1812.5 61 1585.0 62 1352.0 63 1119.0 64 917.0 65 715.0 66 596.0 67 477.0 68 413.5 69 350.0 70 271.0 71 192.0 72 158.5 73 125.0 74 103.5 75 66.0 76 50.0 77 36.0 78 22.0 79 17.5 80 13.0 81 10.5 82 8.0 83 6.5 84 5.0 85 3.0 86 1.0 87 1.5 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 94335.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.87788201621879 #Duplication Level Percentage of deduplicated Percentage of total 1 84.60165767041265 60.809879684104516 2 9.36495295401587 13.462659670323845 3 3.3566351060378135 7.238034663698521 4 1.3287909624517005 3.820427200932846 5 0.5589475857593723 2.008798431123125 6 0.2772615992685013 1.1957385911909684 7 0.12978202518951126 0.6529919966078337 8 0.08701294870660413 0.5003445168813272 9 0.053092646668436425 0.34345682938463984 >10 0.22121936111848509 2.7063126093178567 >50 0.008848774444739404 0.4621826469497005 >100 0.007373978703949503 0.8427412943234218 >500 0.0014747957407899007 0.7134149573329093 >1k 0.0029495914815798013 5.243016907828483 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3083 3.268140138866805 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1863 1.974876768961679 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 673 0.7134149573329093 No Hit CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 295 0.3127153230508295 No Hit GAATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTC 135 0.14310701224359992 No Hit CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 129 0.13674670058832883 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCG 119 0.12614618116287699 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 117 0.12402607727778663 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06572322043780145 0.0 2 0.0 0.0 0.0 0.23109132347485026 0.0 3 0.0 0.0 0.0 0.2989346477977421 0.0 4 0.0 0.0 0.0 0.4462818678115228 0.0 5 0.0 0.0 0.0 0.9434462288652143 0.0 6 0.0 0.0 0.0 1.2646419674564053 0.0 7 0.0 0.0 0.0 1.6261196798643134 0.0 8 0.0 0.0 0.0 2.342714793024858 0.0 9 0.0 0.0 0.0 2.7402342714793027 0.0 10 0.0 0.0 0.0 3.713361954735782 0.0 11 0.0 0.0 0.0 4.195685588593841 0.0 12 0.0 0.0 0.0 4.858218052684582 0.0 13 0.0 0.0 0.0 5.044787194572534 0.0 14 0.0 0.0 0.0 5.167753219907776 0.0 15 0.0 0.0 0.0 5.333121322944824 0.0 16 0.0 0.0 0.0 5.520750516775322 0.0 17 0.0 0.0 0.0 5.751841840250172 0.0 18 0.0 0.0 0.0 6.003074150633381 0.0 19 0.0 0.0 0.0 6.169502305612975 0.0 20 0.0 0.0 0.0 6.3974134732601895 0.0 21 0.0 0.0 0.0 6.577622303492872 0.0 22 0.0 0.0 0.0 6.798113107542269 0.0 23 0.0 0.0 0.0 7.004823236338581 0.0 24 0.0 0.0 0.0 7.187152170456352 0.0 25 0.0 0.0 0.0 7.369481104574124 0.0 26 0.0 0.0 0.0 7.528488895955902 0.0 27 0.0 0.0 0.0 7.72035829755658 0.0 28 0.0 0.0 0.0 7.909047543329623 0.0 29 0.0 0.0 0.0 8.100916944930301 0.0 30 0.0 0.0 0.0 8.37653044999205 0.0 31 0.0 0.0 0.0 8.588540838501087 0.0 32 0.0 0.0 0.0 8.776170032331585 0.0 33 0.0 0.0 0.0 8.989240472783166 0.0 34 0.0 0.0 0.0 9.223511952085651 0.0 35 0.0 0.0 0.0 9.463083691100865 0.0 36 0.0 0.0 0.0 9.696295118460805 0.0 37 0.0 0.0 0.0 9.924206286108019 0.0 38 0.0 0.0 0.0 10.187099167859225 0.0 39 0.0 0.0 0.0 10.541156516669316 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTATT 360 0.0 45.000004 1 TAATCAA 55 1.8189894E-12 45.0 13 TTAGGAT 20 7.0003583E-4 45.0 5 GGTACCT 20 7.0003583E-4 45.0 8 CAACGCA 55 1.8189894E-12 45.0 17 GCTATTA 20 7.0003583E-4 45.0 1 TCAGGGC 25 3.863537E-5 45.0 4 ATCAACG 55 1.8189894E-12 45.0 15 GATATAG 20 7.0003583E-4 45.0 2 CGCACTT 50 2.1827873E-11 45.0 35 TGGGCGA 25 3.863537E-5 45.0 6 TAGGATA 20 7.0003583E-4 45.0 6 CGCAAGC 55 1.8189894E-12 45.0 20 TAATGGG 25 3.863537E-5 45.0 3 CCGCACT 50 2.1827873E-11 45.0 34 TCAACGC 55 1.8189894E-12 45.0 16 TACGGCT 30 2.1442975E-6 44.999996 7 ACAGGGT 30 2.1442975E-6 44.999996 5 ACGGCTG 30 2.1442975E-6 44.999996 8 GCGGGGT 30 2.1442975E-6 44.999996 5 >>END_MODULE