##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936191.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	94335
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52276461546616	31.0	31.0	34.0	30.0	34.0
2	31.660497164361054	31.0	31.0	34.0	30.0	34.0
3	30.27167011183548	31.0	30.0	34.0	25.0	34.0
4	34.79940637091217	35.0	35.0	37.0	32.0	37.0
5	31.249843642338476	35.0	35.0	37.0	0.0	37.0
6	33.49852122754015	36.0	35.0	37.0	17.0	37.0
7	21.756887687496686	32.0	0.0	37.0	0.0	37.0
8	28.62864260348757	35.0	17.0	37.0	17.0	37.0
9	35.05978692955955	37.0	32.0	39.0	32.0	39.0
10	36.52346424974824	37.0	35.0	39.0	33.0	39.0
11	36.91735835055918	39.0	37.0	39.0	34.0	39.0
12	37.31397678486246	39.0	37.0	39.0	34.0	39.0
13	37.19098955848837	39.0	37.0	39.0	34.0	39.0
14	38.68525997773891	40.0	38.0	41.0	35.0	41.0
15	38.76362961785127	40.0	38.0	41.0	35.0	41.0
16	38.78006042296072	40.0	38.0	41.0	35.0	41.0
17	38.75483118672815	40.0	38.0	41.0	35.0	41.0
18	38.44063179095776	40.0	38.0	41.0	35.0	41.0
19	38.12278581650501	40.0	37.0	41.0	34.0	41.0
20	37.79313086341231	39.0	35.0	41.0	34.0	41.0
21	37.66581862511263	39.0	35.0	41.0	33.0	41.0
22	37.473896220914824	39.0	35.0	40.0	33.0	41.0
23	37.28541898553029	38.0	35.0	40.0	33.0	41.0
24	36.99137117718768	38.0	35.0	40.0	33.0	41.0
25	36.82893941803148	38.0	35.0	40.0	33.0	41.0
26	36.68793130863412	38.0	35.0	40.0	33.0	41.0
27	36.60024381194678	38.0	35.0	40.0	33.0	41.0
28	36.495733290931256	38.0	35.0	40.0	33.0	41.0
29	36.375491599088356	38.0	35.0	40.0	32.0	41.0
30	36.10927015423756	38.0	35.0	40.0	31.0	41.0
31	35.81570996978852	38.0	34.0	40.0	30.0	41.0
32	35.361117294747444	38.0	34.0	40.0	27.0	41.0
33	34.612296602533526	37.0	33.0	40.0	23.0	41.0
34	33.65321460751577	37.0	33.0	40.0	18.0	41.0
35	32.68510096994753	37.0	31.0	40.0	12.0	41.0
36	32.012201197858694	37.0	30.0	40.0	9.0	41.0
37	31.587671595908198	37.0	28.0	40.0	8.0	41.0
38	31.256511369057083	37.0	25.0	40.0	8.0	41.0
39	31.038236073567603	36.0	25.0	40.0	7.0	41.0
40	30.893167965230298	36.0	24.0	40.0	7.0	41.0
41	30.757332909312556	36.0	24.0	40.0	7.0	41.0
42	30.58158689775799	36.0	23.0	40.0	7.0	41.0
43	30.426511899083057	36.0	23.0	40.0	7.0	41.0
44	30.21197858695076	35.0	22.0	40.0	7.0	41.0
45	30.094588434833305	35.0	22.0	40.0	7.0	41.0
46	29.943955053797637	35.0	22.0	40.0	7.0	41.0
47	29.900863942333174	35.0	22.0	40.0	7.0	41.0
48	29.776170032331585	35.0	20.0	40.0	7.0	41.0
49	29.636677797212062	35.0	20.0	40.0	7.0	41.0
50	29.476249536227275	35.0	20.0	40.0	7.0	41.0
51	28.023533153124504	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	5.0
10	1.0
11	3.0
12	0.0
13	1.0
14	1.0
15	6.0
16	5.0
17	25.0
18	43.0
19	110.0
20	226.0
21	415.0
22	760.0
23	1373.0
24	2349.0
25	3662.0
26	4553.0
27	4507.0
28	3823.0
29	3171.0
30	2824.0
31	2863.0
32	3238.0
33	4366.0
34	5184.0
35	6950.0
36	8120.0
37	13111.0
38	13604.0
39	9031.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.095934700800342	15.839296125510149	18.884825356442466	35.17994381724704
2	49.124927121428954	19.84099220861822	19.279164679069275	11.754915990883553
3	24.532782106323207	19.937456935389832	44.01759686224625	11.512164096040706
4	19.824031377537498	18.94630836911009	47.85604494620237	13.37361530715005
5	17.19828271585308	31.328775109980388	38.94630836911009	12.526633805056447
6	17.936078867864524	26.56490168018233	45.06492818148089	10.434091270472253
7	35.81173477500397	39.384109821381244	22.52928393491281	2.274871468701966
8	60.716595113160544	4.8073355594424125	31.697673185986115	2.778396141410929
9	56.18275295489479	6.018974929771559	32.896591933004714	4.901680182328934
10	27.061005989293474	24.955742831398737	38.37600042402078	9.607250755287009
11	18.797901097153762	20.040281973816718	48.134838607091744	13.026978321937774
12	17.609582869560608	18.90072613558064	49.327397042455075	14.162293952403667
13	16.650235861557217	18.99189060263953	50.950336566491764	13.407536969311495
14	15.154502570625962	21.32506492818148	49.08676525149732	14.433667249695233
15	13.94392325223936	21.779827211533366	50.75316690517835	13.523082631048922
16	17.258705676578153	21.344145863147293	48.032013568664865	13.36513489160969
17	17.30110775427996	20.943446228865213	47.56453066200243	14.190915354852388
18	17.29050723485451	21.29856362961785	48.03837388032014	13.372555255207505
19	16.20077383791806	22.553665129591348	46.62956484867758	14.615996183813007
20	16.551651030900512	22.70313245349022	47.48502676631155	13.260189749297716
21	17.075316690517838	22.401017649864844	47.745799544177665	12.777866115439657
22	16.741400328616102	19.989399480574548	47.84968463454709	15.419515556262256
23	15.003975194784545	21.942015158742777	48.56839985159273	14.48560979487995
24	15.461917633964065	21.612339004611226	47.77972120633911	15.1460221550856
25	15.593364074839666	23.715482058620875	46.30730905814385	14.383844808395613
26	15.300789738697196	22.565325700959345	46.56914188795251	15.564742672390947
27	15.738591190968357	21.550855991943603	47.58679175279589	15.123761064292152
28	14.380664652567976	22.215508559919435	48.01929294535432	15.384533842158266
29	16.84952562675571	21.464991784597444	46.48327768060635	15.202204908040496
30	16.08840833200827	21.53707531669052	48.11363756824084	14.26087878306037
31	17.323368845073407	21.14697620183389	46.61896432925213	14.91069062384057
32	17.28838713096942	21.685482588646842	46.08575820215191	14.940372078231833
33	16.401123655059095	22.217628663804526	45.26633805056448	16.114909630571898
34	16.251656331160227	23.15895478878465	45.28859914135793	15.300789738697196
35	15.898659034292681	24.756453066200244	43.41972756665077	15.925160332856311
36	17.612763025388244	25.28117877776011	40.978427942969205	16.12763025388244
37	16.44882599247363	26.206604123602055	40.62437059415912	16.720199289765198
38	17.341389728096676	27.725658557269306	38.72687761700323	16.206074097630786
39	17.886256426564902	26.07197752689882	38.28377590501935	17.757990141516935
40	17.745269518206392	26.687867705517572	38.97174961573117	16.595113160544866
41	16.938569989929505	26.252186357131503	38.19897174961573	18.610271903323262
42	17.682726453596224	26.32426988922457	38.355859437112414	17.637144220066784
43	17.87141569936927	25.606614724121478	37.80993268670165	18.7120368898076
44	18.61981237080617	26.02957544919701	37.46647585731701	17.88413632267981
45	18.136428685005566	25.92251020299995	36.71595908199502	19.22510202999947
46	18.74065829225632	26.517199342767796	36.492288122117984	18.2498542428579
47	17.25022526103779	25.87904807335559	38.38130068373351	18.48942598187311
48	17.637144220066784	26.651825939471035	36.807123549053905	18.90390629140828
49	17.73890920655112	25.526050776488045	38.095086659246306	18.63995335771453
50	16.98203211957386	25.60449462023639	37.94561933534743	19.46785392484232
51	17.071076482747653	25.257857635024116	37.34562993586686	20.325435946361374
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	150.0
1	213.0
2	276.0
3	4617.5
4	8959.0
5	6096.0
6	3233.0
7	2801.0
8	2369.0
9	2235.0
10	2101.0
11	2029.0
12	1957.0
13	1794.5
14	1632.0
15	1623.0
16	1614.0
17	1451.5
18	1289.0
19	1243.5
20	1198.0
21	1156.0
22	1114.0
23	1110.0
24	1106.0
25	1173.5
26	1345.0
27	1449.0
28	1628.0
29	1807.0
30	1978.5
31	2150.0
32	2450.5
33	2751.0
34	3033.5
35	3316.0
36	3507.5
37	3699.0
38	3820.5
39	3942.0
40	4085.5
41	4229.0
42	4442.0
43	4655.0
44	4999.0
45	5343.0
46	5712.0
47	6081.0
48	5782.5
49	5484.0
50	5189.0
51	4894.0
52	4342.0
53	3790.0
54	3500.5
55	3211.0
56	2859.5
57	2508.0
58	2274.0
59	2040.0
60	1812.5
61	1585.0
62	1352.0
63	1119.0
64	917.0
65	715.0
66	596.0
67	477.0
68	413.5
69	350.0
70	271.0
71	192.0
72	158.5
73	125.0
74	103.5
75	66.0
76	50.0
77	36.0
78	22.0
79	17.5
80	13.0
81	10.5
82	8.0
83	6.5
84	5.0
85	3.0
86	1.0
87	1.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	94335.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.87788201621879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.60165767041265	60.809879684104516
2	9.36495295401587	13.462659670323845
3	3.3566351060378135	7.238034663698521
4	1.3287909624517005	3.820427200932846
5	0.5589475857593723	2.008798431123125
6	0.2772615992685013	1.1957385911909684
7	0.12978202518951126	0.6529919966078337
8	0.08701294870660413	0.5003445168813272
9	0.053092646668436425	0.34345682938463984
>10	0.22121936111848509	2.7063126093178567
>50	0.008848774444739404	0.4621826469497005
>100	0.007373978703949503	0.8427412943234218
>500	0.0014747957407899007	0.7134149573329093
>1k	0.0029495914815798013	5.243016907828483
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3083	3.268140138866805	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1863	1.974876768961679	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	673	0.7134149573329093	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	295	0.3127153230508295	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTC	135	0.14310701224359992	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	129	0.13674670058832883	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCG	119	0.12614618116287699	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	117	0.12402607727778663	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06572322043780145	0.0
2	0.0	0.0	0.0	0.23109132347485026	0.0
3	0.0	0.0	0.0	0.2989346477977421	0.0
4	0.0	0.0	0.0	0.4462818678115228	0.0
5	0.0	0.0	0.0	0.9434462288652143	0.0
6	0.0	0.0	0.0	1.2646419674564053	0.0
7	0.0	0.0	0.0	1.6261196798643134	0.0
8	0.0	0.0	0.0	2.342714793024858	0.0
9	0.0	0.0	0.0	2.7402342714793027	0.0
10	0.0	0.0	0.0	3.713361954735782	0.0
11	0.0	0.0	0.0	4.195685588593841	0.0
12	0.0	0.0	0.0	4.858218052684582	0.0
13	0.0	0.0	0.0	5.044787194572534	0.0
14	0.0	0.0	0.0	5.167753219907776	0.0
15	0.0	0.0	0.0	5.333121322944824	0.0
16	0.0	0.0	0.0	5.520750516775322	0.0
17	0.0	0.0	0.0	5.751841840250172	0.0
18	0.0	0.0	0.0	6.003074150633381	0.0
19	0.0	0.0	0.0	6.169502305612975	0.0
20	0.0	0.0	0.0	6.3974134732601895	0.0
21	0.0	0.0	0.0	6.577622303492872	0.0
22	0.0	0.0	0.0	6.798113107542269	0.0
23	0.0	0.0	0.0	7.004823236338581	0.0
24	0.0	0.0	0.0	7.187152170456352	0.0
25	0.0	0.0	0.0	7.369481104574124	0.0
26	0.0	0.0	0.0	7.528488895955902	0.0
27	0.0	0.0	0.0	7.72035829755658	0.0
28	0.0	0.0	0.0	7.909047543329623	0.0
29	0.0	0.0	0.0	8.100916944930301	0.0
30	0.0	0.0	0.0	8.37653044999205	0.0
31	0.0	0.0	0.0	8.588540838501087	0.0
32	0.0	0.0	0.0	8.776170032331585	0.0
33	0.0	0.0	0.0	8.989240472783166	0.0
34	0.0	0.0	0.0	9.223511952085651	0.0
35	0.0	0.0	0.0	9.463083691100865	0.0
36	0.0	0.0	0.0	9.696295118460805	0.0
37	0.0	0.0	0.0	9.924206286108019	0.0
38	0.0	0.0	0.0	10.187099167859225	0.0
39	0.0	0.0	0.0	10.541156516669316	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	360	0.0	45.000004	1
TAATCAA	55	1.8189894E-12	45.0	13
TTAGGAT	20	7.0003583E-4	45.0	5
GGTACCT	20	7.0003583E-4	45.0	8
CAACGCA	55	1.8189894E-12	45.0	17
GCTATTA	20	7.0003583E-4	45.0	1
TCAGGGC	25	3.863537E-5	45.0	4
ATCAACG	55	1.8189894E-12	45.0	15
GATATAG	20	7.0003583E-4	45.0	2
CGCACTT	50	2.1827873E-11	45.0	35
TGGGCGA	25	3.863537E-5	45.0	6
TAGGATA	20	7.0003583E-4	45.0	6
CGCAAGC	55	1.8189894E-12	45.0	20
TAATGGG	25	3.863537E-5	45.0	3
CCGCACT	50	2.1827873E-11	45.0	34
TCAACGC	55	1.8189894E-12	45.0	16
TACGGCT	30	2.1442975E-6	44.999996	7
ACAGGGT	30	2.1442975E-6	44.999996	5
ACGGCTG	30	2.1442975E-6	44.999996	8
GCGGGGT	30	2.1442975E-6	44.999996	5
>>END_MODULE