##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936187.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	264115
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65608920356663	31.0	31.0	34.0	30.0	34.0
2	31.816829790053575	31.0	31.0	34.0	30.0	34.0
3	31.11352251859985	31.0	31.0	34.0	27.0	34.0
4	35.23210722601897	37.0	35.0	37.0	33.0	37.0
5	31.70652935274407	35.0	35.0	37.0	0.0	37.0
6	33.72126914412283	37.0	35.0	37.0	18.0	37.0
7	21.102319065558564	32.0	0.0	37.0	0.0	37.0
8	28.270332998883063	35.0	17.0	37.0	17.0	37.0
9	34.7649622323609	37.0	32.0	39.0	30.0	39.0
10	36.21907123790773	37.0	35.0	39.0	32.0	39.0
11	36.69223255021487	38.0	35.0	39.0	33.0	39.0
12	37.09100581186226	39.0	37.0	39.0	34.0	39.0
13	36.95952899305227	39.0	37.0	39.0	33.0	39.0
14	38.408977150105066	40.0	38.0	41.0	34.0	41.0
15	38.53302917289817	40.0	38.0	41.0	34.0	41.0
16	38.55825681994585	40.0	38.0	41.0	34.0	41.0
17	38.51532476383394	40.0	38.0	41.0	34.0	41.0
18	38.301452019006874	40.0	38.0	41.0	34.0	41.0
19	38.15512560816311	40.0	37.0	41.0	34.0	41.0
20	37.98862616663196	40.0	36.0	41.0	34.0	41.0
21	37.897283380345684	40.0	36.0	41.0	33.0	41.0
22	37.80776555667039	39.0	36.0	41.0	33.0	41.0
23	37.699081839350285	39.0	35.0	41.0	33.0	41.0
24	37.541782935463715	39.0	35.0	41.0	33.0	41.0
25	37.40117373113984	39.0	35.0	41.0	33.0	41.0
26	37.25745224618064	39.0	35.0	41.0	33.0	41.0
27	37.20207863998637	39.0	35.0	41.0	33.0	41.0
28	37.02403877098991	39.0	35.0	41.0	33.0	41.0
29	36.98092118963331	39.0	35.0	41.0	33.0	41.0
30	36.86040550517767	39.0	35.0	41.0	32.0	41.0
31	36.65587338848608	39.0	35.0	40.0	31.0	41.0
32	36.44800181739015	39.0	35.0	40.0	30.0	41.0
33	36.097749086572136	39.0	35.0	40.0	30.0	41.0
34	35.65520322586752	39.0	35.0	40.0	25.0	41.0
35	35.264210665808456	39.0	35.0	40.0	24.0	41.0
36	34.89337220528936	38.0	34.0	40.0	21.0	41.0
37	34.75023001344111	38.0	34.0	40.0	21.0	41.0
38	34.553357438994375	38.0	34.0	40.0	19.0	41.0
39	34.487302879427524	38.0	34.0	40.0	18.0	41.0
40	34.325009938852396	38.0	33.0	40.0	18.0	41.0
41	34.17897506767885	38.0	33.0	40.0	18.0	41.0
42	34.057028188478505	38.0	33.0	40.0	16.0	41.0
43	33.90757813831096	38.0	33.0	40.0	15.0	41.0
44	33.75015050262196	38.0	33.0	40.0	15.0	41.0
45	33.68386119682714	38.0	33.0	40.0	15.0	41.0
46	33.50125134884426	38.0	33.0	40.0	14.0	41.0
47	33.41887814020408	38.0	33.0	40.0	13.0	41.0
48	33.25958010715029	38.0	32.0	40.0	13.0	41.0
49	33.07826514965072	37.0	32.0	40.0	11.0	41.0
50	32.91219733828067	37.0	32.0	40.0	10.0	41.0
51	31.058220850765764	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	7.0
11	5.0
12	2.0
13	9.0
14	3.0
15	9.0
16	17.0
17	38.0
18	90.0
19	233.0
20	429.0
21	844.0
22	1355.0
23	2151.0
24	3637.0
25	5260.0
26	6564.0
27	6674.0
28	6372.0
29	6007.0
30	6479.0
31	7403.0
32	9430.0
33	12764.0
34	17085.0
35	22581.0
36	27321.0
37	44275.0
38	46410.0
39	30646.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.86468015826439	19.24805482460292	23.11682411070935	21.77044090642334
2	38.9451564659334	23.953959449482234	23.152793290801355	13.948090793783011
3	28.678416598830054	23.02254699657346	34.45393105276111	13.845105351835373
4	23.855138859966303	23.16339473335479	36.537114514510726	16.444351892168186
5	20.16470098252655	34.79923518164436	29.705242034719724	15.330821801109366
6	21.614826874656874	32.07390719951536	33.75385722128618	12.557408704541581
7	42.703746474073796	41.77157677526835	12.87242299755788	2.6522537530999752
8	73.32184843723378	5.379474849970657	18.164435946462714	3.134240766332847
9	67.07911326505499	7.83900952236715	19.805766427503173	5.276110785074684
10	30.927436911951233	32.00954129829809	25.73765215909736	11.325369630653313
11	22.685193949605285	25.73992389678739	36.690835431535504	14.884046722071826
12	22.357685099293867	22.54775381935899	39.17422334967722	15.920337731669918
13	20.141604982678	23.566628173333587	39.99318478692994	16.29858205705848
14	16.24708933608466	27.590632868258147	38.10385627472881	18.058421520928384
15	15.844991764950874	26.36351589269826	41.85866005338584	15.932832288965034
16	18.368135092667966	26.084092156825626	38.705109516687806	16.8426632338186
17	18.68996459875433	24.691138329894173	36.808208545519946	19.81068852583155
18	19.759195804857733	25.759612290100904	37.406811426840584	17.074380478200784
19	20.35174071900498	26.00193097703652	36.12895897620355	17.517369327754956
20	20.770876322813926	24.881964295855973	37.25839123109252	17.088768150237584
21	20.41875698086061	25.911061469435666	37.31064119796301	16.35954035174072
22	19.490373511538532	24.446926528216874	36.86803097135717	19.19466898888742
23	18.1129432254889	26.050394714423643	37.1822880184768	18.654374041610662
24	17.94294152168563	24.05543039963652	38.796357647237	19.205270431440848
25	17.771046703140676	27.268424739223445	36.31902769626867	18.64150086136721
26	17.46511936088446	27.236999034511484	36.35386100751566	18.94402059708839
27	19.026181776877497	26.198057664275034	36.9093008727259	17.866459686121576
28	17.25195464097079	25.72326448706056	38.97998977718039	18.044791094788255
29	18.23334532306003	26.430153531605548	36.865001987770476	18.47149915756394
30	19.244647218067886	25.731594191923975	37.36402703367851	17.659731556329632
31	19.586543740416108	26.73153739848172	36.12138651723681	17.56053234386536
32	20.02082426215853	27.60615640913996	36.0782235011264	16.294795827575108
33	19.89966491869072	26.82202828313424	35.44175832497208	17.836548473202964
34	19.442288397099748	26.548662514435	36.52272684247392	17.486322245991328
35	19.995835147568293	25.48170304602162	36.000227173769	18.522234632641084
36	20.260113965507447	28.236184995172557	34.88291085322681	16.620790186093178
37	20.370293243473487	27.746625522972945	35.07146508149859	16.811616152054974
38	19.6315998712682	28.398992862957424	33.49790810821044	18.47149915756394
39	21.76438293924995	26.618329136928985	32.90612043995987	18.711167483861196
40	20.943528387255554	26.82430002082426	34.87344527951839	17.358726312401796
41	19.150748726880337	27.648183556405353	33.86327925335554	19.337788463358766
42	20.229445506692162	27.269560608068456	33.917800957916064	18.583192927323324
43	21.20023474622797	26.739867103345134	32.96139939041705	19.098498760009843
44	19.8758116729455	27.23359142797645	32.47865513128751	20.411941767790545
45	19.532779281752266	27.532703557162602	31.451829695397837	21.482687465687295
46	19.976146754254774	28.095715881339565	31.972814872309414	19.955322492096244
47	20.108286163224353	26.200329401965057	34.459610396986164	19.23177403782443
48	19.254491414724647	26.41197963008538	34.18170115290688	20.151827802283094
49	19.182931677488973	26.507392613066276	34.31686954546315	19.992806163981598
50	18.435151354523597	26.126119304091024	34.75569354258561	20.683035798799764
51	18.86943187626602	25.499498324593457	32.95647729208868	22.67459250705185
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	880.0
1	833.5
2	787.0
3	6783.5
4	12780.0
5	8704.5
6	4629.0
7	3963.0
8	3297.0
9	3064.0
10	2831.0
11	2720.5
12	2610.0
13	2483.0
14	2356.0
15	2270.0
16	2184.0
17	2117.5
18	2051.0
19	2039.5
20	2028.0
21	2137.0
22	2246.0
23	2330.5
24	2415.0
25	2799.0
26	3703.0
27	4223.0
28	4886.5
29	5550.0
30	6381.5
31	7213.0
32	8021.0
33	8829.0
34	9450.5
35	10072.0
36	10414.5
37	10757.0
38	11608.0
39	12459.0
40	13404.0
41	14349.0
42	15185.0
43	16021.0
44	17618.5
45	19216.0
46	20920.5
47	22625.0
48	21099.0
49	19573.0
50	18032.0
51	16491.0
52	14221.5
53	11952.0
54	10559.5
55	9167.0
56	8470.5
57	7774.0
58	7288.5
59	6803.0
60	6340.0
61	5877.0
62	5091.5
63	4306.0
64	3439.0
65	2572.0
66	2036.5
67	1501.0
68	1237.5
69	974.0
70	778.5
71	583.0
72	517.0
73	451.0
74	357.5
75	185.0
76	106.0
77	83.0
78	60.0
79	45.5
80	31.0
81	26.5
82	22.0
83	15.0
84	8.0
85	5.0
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	264115.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.821817946582385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.87103671311264	39.83596869911974
2	8.729182605184448	9.047242235766841
3	4.1235500452074225	6.410695792090817
4	2.625571901171118	5.442476362726075
5	1.8727739877402467	4.852527632388509
6	1.4022417699330223	4.360003063113752
7	1.0064307849066902	3.650855103788935
8	0.7308756281560442	3.0300242995117252
9	0.6307179936895942	2.9416457740154263
>10	1.9531599539400382	13.841904518451159
>50	0.03311672652895288	1.1681390144044828
>100	0.01766225414877487	1.7985450838823185
>500	0.0014718545123979057	0.4610773974583806
>1k	0.0022077817685968587	3.158895023281858
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4602	1.7424228082464077	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2670	1.0109232720595196	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1011	0.3827878007686046	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	678	0.25670635897241734	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	531	0.2010487855668932	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTC	472	0.1787100316150162	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCG	416	0.15750714650814987	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGC	349	0.13213940896957765	No Hit
GAATCTATCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTC	322	0.12191658936448138	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCG	308	0.11661586808776479	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.786229483368987E-4	0.0	0.0	0.09389849118755088	0.0
2	3.786229483368987E-4	0.0	0.0	0.3615849156617383	0.0
3	3.786229483368987E-4	0.0	0.0	0.5198493080665619	0.0
4	3.786229483368987E-4	0.0	0.0	0.6955303560948829	0.0
5	3.786229483368987E-4	0.0	0.0	1.2683868769286106	0.0
6	3.786229483368987E-4	0.0	0.0	1.734093103382996	0.0
7	3.786229483368987E-4	0.0	0.0	2.0903772977680175	0.0
8	3.786229483368987E-4	0.0	0.0	2.784014539121216	0.0
9	3.786229483368987E-4	0.0	0.0	3.0630596520455105	0.0
10	3.786229483368987E-4	0.0	0.0	3.9766768263824472	0.0
11	3.786229483368987E-4	0.0	0.0	4.730136493572876	0.0
12	3.786229483368987E-4	0.0	0.0	5.60854173371448	0.0
13	3.786229483368987E-4	0.0	0.0	5.9769418624462824	0.0
14	3.786229483368987E-4	0.0	0.0	6.160195369441342	0.0
15	3.786229483368987E-4	0.0	0.0	6.410086515343695	0.0
16	3.786229483368987E-4	0.0	0.0	6.878443102436439	0.0
17	3.786229483368987E-4	0.0	0.0	7.413815951384813	0.0
18	3.786229483368987E-4	0.0	0.0	8.051038373435814	0.0
19	3.786229483368987E-4	0.0	0.0	8.40315771538913	0.0
20	3.786229483368987E-4	0.0	0.0	8.754898434394109	0.0
21	3.786229483368987E-4	0.0	0.0	9.194479677413248	0.0
22	3.786229483368987E-4	0.0	0.0	9.618537379550574	0.0
23	3.786229483368987E-4	0.0	0.0	10.060390360259735	0.0
24	3.786229483368987E-4	0.0	0.0	10.404179997349638	0.0
25	3.786229483368987E-4	0.0	0.0	10.74115442136948	0.0
26	3.786229483368987E-4	0.0	0.0	11.046703140677357	0.0
27	3.786229483368987E-4	0.0	0.0	11.375726482782122	0.0
28	3.786229483368987E-4	0.0	0.0	11.68733316926339	0.0
29	3.786229483368987E-4	0.0	0.0	12.016356511368153	0.0
30	3.786229483368987E-4	0.0	0.0	12.398008443291747	0.0
31	3.786229483368987E-4	0.0	0.0	12.74937053934839	0.0
32	3.786229483368987E-4	0.0	0.0	13.138973553187059	0.0
33	3.786229483368987E-4	0.0	0.0	13.493364632830396	0.0
34	3.786229483368987E-4	0.0	0.0	13.851541941957102	0.0
35	3.786229483368987E-4	0.0	0.0	14.240387709899098	0.0
36	3.786229483368987E-4	0.0	0.0	14.60500160914753	0.0
37	3.786229483368987E-4	0.0	0.0	14.974158983776007	0.0
38	3.786229483368987E-4	0.0	0.0	15.370198587736402	0.0
39	3.786229483368987E-4	0.0	0.0	15.75147189671166	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTTA	65	0.0	45.000004	1
TTTAGCG	20	7.02341E-4	45.0	1
TTAGGCG	20	7.02341E-4	45.0	5
TCGCAAA	20	7.02341E-4	45.0	1
GGCACCG	35	1.2071723E-7	45.0	8
CGTGACA	20	7.02341E-4	45.0	26
GGACTAC	20	7.02341E-4	45.0	8
CACGCCG	20	7.02341E-4	45.0	26
CTACGGG	20	7.02341E-4	45.0	3
ACGTGAC	20	7.02341E-4	45.0	25
TTGCGCA	25	3.8826212E-5	45.0	1
GAGCCCC	25	3.8826212E-5	45.0	37
CAATGGC	20	7.02341E-4	45.0	16
TGTAACG	30	2.1591313E-6	44.999996	1
CGTTTTA	955	0.0	44.293194	1
CGTTATT	445	0.0	43.988766	1
CGTTTTT	1730	0.0	43.439304	1
ACAACGA	70	0.0	41.785713	13
AGCGACT	70	0.0	41.785713	19
CACAACG	70	0.0	41.785713	12
>>END_MODULE