##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936186.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	169003
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.696632604154956	31.0	31.0	34.0	30.0	34.0
2	31.84465956225629	31.0	31.0	34.0	30.0	34.0
3	31.39835979242972	31.0	31.0	34.0	28.0	34.0
4	35.3918747004491	37.0	35.0	37.0	33.0	37.0
5	31.772542499245574	37.0	35.0	37.0	0.0	37.0
6	33.706123559936806	37.0	35.0	37.0	18.0	37.0
7	21.143240060827324	32.0	0.0	37.0	0.0	37.0
8	28.095465760962824	35.0	17.0	37.0	17.0	37.0
9	34.490080057750454	35.0	32.0	39.0	28.0	39.0
10	36.14683763010124	37.0	35.0	39.0	32.0	39.0
11	36.56236871534825	38.0	35.0	39.0	32.0	39.0
12	36.884865949125164	39.0	35.0	39.0	33.0	39.0
13	36.61172878587954	39.0	35.0	39.0	32.0	39.0
14	38.10200410643598	40.0	37.0	41.0	33.0	41.0
15	38.16771891623225	40.0	37.0	41.0	33.0	41.0
16	38.2707289219718	40.0	37.0	41.0	34.0	41.0
17	38.13926971710561	40.0	37.0	41.0	33.0	41.0
18	37.95150973651355	40.0	37.0	41.0	33.0	41.0
19	37.79926391839198	40.0	37.0	41.0	33.0	41.0
20	37.77008100447921	39.0	36.0	41.0	33.0	41.0
21	37.74693348638782	39.0	36.0	41.0	33.0	41.0
22	37.72562617231647	39.0	36.0	41.0	33.0	41.0
23	37.68859724383591	39.0	35.0	41.0	33.0	41.0
24	37.47553002017715	39.0	35.0	41.0	33.0	41.0
25	37.37749625746289	39.0	35.0	41.0	33.0	41.0
26	37.159843316390834	39.0	35.0	41.0	33.0	41.0
27	37.13750643479702	39.0	35.0	40.0	33.0	41.0
28	37.07727081767779	39.0	35.0	40.0	32.0	41.0
29	36.93239173269114	39.0	35.0	40.0	32.0	41.0
30	36.69360898918954	39.0	35.0	40.0	31.0	41.0
31	36.55067661520801	39.0	35.0	40.0	31.0	41.0
32	36.22794269924203	38.0	35.0	40.0	30.0	41.0
33	36.01944935888712	38.0	35.0	40.0	30.0	41.0
34	35.679159541546596	38.0	35.0	40.0	27.0	41.0
35	35.19619770063253	38.0	34.0	40.0	24.0	41.0
36	35.05327716076046	38.0	34.0	40.0	24.0	41.0
37	34.759607817612704	38.0	34.0	40.0	22.0	41.0
38	34.698691739199894	38.0	34.0	40.0	22.0	41.0
39	34.419844618142875	38.0	33.0	40.0	21.0	41.0
40	34.32660366975734	38.0	33.0	40.0	20.0	41.0
41	34.13725200144376	38.0	33.0	40.0	18.0	41.0
42	34.13964840860813	38.0	33.0	40.0	18.0	41.0
43	33.957065850902055	38.0	33.0	40.0	18.0	41.0
44	33.8489612610427	38.0	33.0	40.0	18.0	41.0
45	33.7546907451347	38.0	33.0	40.0	18.0	41.0
46	33.44580865428424	37.0	32.0	40.0	15.0	41.0
47	33.305905812322855	37.0	32.0	40.0	15.0	41.0
48	33.151600859156346	37.0	32.0	40.0	15.0	41.0
49	33.06692188896055	37.0	31.0	40.0	14.0	41.0
50	32.948628130861586	37.0	31.0	40.0	13.0	41.0
51	30.823766442015824	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	3.0
11	7.0
12	4.0
13	3.0
14	4.0
15	4.0
16	14.0
17	48.0
18	94.0
19	147.0
20	253.0
21	472.0
22	764.0
23	1308.0
24	1974.0
25	2991.0
26	3709.0
27	4074.0
28	4184.0
29	4272.0
30	4716.0
31	5548.0
32	6841.0
33	9292.0
34	12235.0
35	15453.0
36	17395.0
37	27304.0
38	27769.0
39	18109.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71227729685272	18.160624367614776	21.39251965941433	18.734578676118176
2	34.721277137092244	30.480524014366612	20.375969657343358	14.422229191197788
3	28.635586350538155	28.550380762471672	28.571090454015607	14.242942432974562
4	25.35162097714242	22.185404992810778	36.93721413229351	15.525759897753295
5	23.757566433731945	33.752063572835986	26.078827003070952	16.411542990361117
6	22.758767595841494	35.591084181937596	29.453323313787326	12.196824908433577
7	41.3708632391141	41.751921563522544	13.86661775234759	3.010597445015769
8	67.09762548593812	10.880280231711863	17.448802683976027	4.573291598373993
9	61.171103471536	9.244806305213517	17.831636124802518	11.752454098447956
10	35.89403738395177	24.837428921380095	25.272924149275454	13.995609545392684
11	28.629077590338632	22.135110027632646	31.966296456275924	17.2695159257528
12	27.069341964343828	20.816198529020195	35.09227646846506	17.022183038170922
13	20.210292124991863	23.62088246954196	38.67327798914812	17.495547416318054
14	17.775424104897546	28.43085625698952	34.008863747980804	19.78485589013213
15	16.500890516736387	24.66110069052029	42.48445293870523	16.353555854038092
16	18.8440441885647	24.556368821855234	35.695224345129965	20.904362644450096
17	18.491387726845083	23.558753394910152	36.68041395714869	21.269444921096074
18	18.889605509961363	26.60721999017769	35.310615787885425	19.192558711975526
19	20.756436276279118	26.654556427992404	31.93966971000515	20.64933758572333
20	21.436305864392942	25.480021064714826	36.25379431134358	16.82987875954865
21	20.749927516079598	28.33440826494204	33.643781471334826	17.271882747643534
22	19.174215842322326	23.963479938225948	33.48106246634675	23.381241753104977
23	19.94994171701094	27.526730294728495	34.448500914184955	18.074827074075607
24	21.415596172849003	23.891883576031194	33.31301811210452	21.379502139015283
25	18.42689183032254	29.560422004343117	32.12369011200985	19.888996053324497
26	18.080744128802447	25.98947947669568	33.80709218179559	22.122684212706282
27	20.80968976882067	27.230877558386535	33.08757832701195	18.871854345780843
28	17.403833068052048	25.620255261740915	36.8454997840275	20.130411886179537
29	23.39307586255865	24.298385235765046	32.78048318668899	19.528055714987307
30	18.289616160659868	27.12437057330343	36.84135784571871	17.744655420317983
31	25.471145482624568	24.543351301456187	30.801820086033977	19.183683129885267
32	22.769418294349805	29.239717638148434	31.156843369644328	16.834020697857436
33	20.47064253297279	27.638562629065756	30.679337053188405	21.211457784773053
34	24.69482790246327	25.376472606995144	31.41246013384378	18.51623935669781
35	19.737519452317414	24.96937924178861	32.578119915031095	22.714981390862885
36	24.820269462672258	26.403081602101736	31.985822736874493	16.790826198351507
37	19.61740324136258	29.198889960533247	33.98815405643687	17.195552741667306
38	21.12625219670657	29.013094442110493	29.00954420927439	20.851109151908545
39	24.29779353029236	26.558700141417606	31.21068856765856	17.932817760631465
40	20.466500594664	26.763430234966247	32.3017934592877	20.46827571108205
41	19.628645645343575	27.284722756400775	33.29053330414253	19.796098294113122
42	19.503795790607267	24.87648148257723	34.14791453406153	21.471808192753976
43	22.01795234404123	25.43564315426353	30.862765749720417	21.68363875197482
44	20.680697975775576	26.33858570557919	31.198854458204885	21.781861860440348
45	19.63101246723431	26.417874238918838	30.35804098152104	23.593072312325816
46	23.52088424465838	29.21427430282303	28.469908818186656	18.794932634331936
47	19.751720383661826	24.860505434814765	36.052614450631054	19.33515973089235
48	21.10080886138116	25.690076507517617	31.976947154784234	21.232167476316988
49	18.503813541771446	25.364046792068777	35.07807553712065	21.05406412903913
50	18.88191333881647	25.297184073655497	33.58756945142986	22.233333136098178
51	19.783080773714076	24.845121092524984	30.201830736732482	25.169967397028454
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	636.0
1	658.5
2	681.0
3	3660.5
4	6640.0
5	4391.5
6	2143.0
7	1819.5
8	1496.0
9	1417.0
10	1338.0
11	1255.5
12	1173.0
13	1163.5
14	1154.0
15	1095.5
16	1037.0
17	1010.5
18	984.0
19	995.0
20	1006.0
21	1166.0
22	1326.0
23	1385.0
24	1444.0
25	1801.5
26	2364.5
27	2570.0
28	2888.5
29	3207.0
30	3616.5
31	4026.0
32	4455.5
33	4885.0
34	5094.0
35	5303.0
36	5576.0
37	5849.0
38	6264.0
39	6679.0
40	7346.5
41	8014.0
42	8666.0
43	9318.0
44	11147.5
45	12977.0
46	15702.0
47	18427.0
48	14732.5
49	11038.0
50	10386.5
51	9735.0
52	8602.5
53	7470.0
54	6981.0
55	6492.0
56	6225.0
57	5958.0
58	5920.5
59	5883.0
60	5727.5
61	5572.0
62	5045.5
63	4519.0
64	3702.0
65	2885.0
66	2359.0
67	1833.0
68	1493.5
69	1154.0
70	984.5
71	815.0
72	653.0
73	491.0
74	385.0
75	221.0
76	163.0
77	148.5
78	134.0
79	95.0
80	56.0
81	34.0
82	12.0
83	14.0
84	16.0
85	13.5
86	11.0
87	10.0
88	9.0
89	5.0
90	1.0
91	0.5
92	0.0
93	2.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	169003.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.98379910415791
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.49512314383509	33.795849777814595
2	9.042128831355997	8.315828713099767
3	4.858841392799238	6.70283959456341
4	3.330159302056258	6.125335053223907
5	2.2801554417479477	5.242510487979503
6	1.7281313534240936	4.767962698887002
7	1.2455928146794657	4.009396282906221
8	1.0023933911521734	3.6875085057661696
9	0.7180173456520061	2.9715448838186305
>10	2.213243431042026	13.86365922498417
>50	0.048897238592788946	1.5905043105743686
>100	0.025735388733046816	2.569185162393567
>500	0.005147077746609363	1.749081377253658
>1k	0.006433847183261704	4.608793926735029
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2369	1.401750264788199	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCG	1448	0.8567895244463115	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	1420	0.8402217712111619	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1348	0.79761897717792	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC	1204	0.7124133891114359	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCG	891	0.5272095761613699	No Hit
GAATCTATCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	783	0.4633053851115069	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC	778	0.4603468577480873	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	504	0.2982195582326941	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	494	0.2923025035058549	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCT	327	0.1934876895676408	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCG	313	0.185203812950066	No Hit
GAATATGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC	277	0.16390241593344498	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTT	270	0.15976047762465756	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCT	262	0.15502683384318622	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	237	0.1402341970260883	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTC	233	0.13786737513535263	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	221	0.13076690946314562	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC	217	0.12840008757240995	No Hit
GAATCTATCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTT	209	0.12366644379093862	No Hit
GAATGAATGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCT	195	0.11538256717336379	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.042011088560558095	0.0
2	0.0	0.0	0.0	0.4005846050070117	0.0
3	0.0	0.0	0.0	0.4810565492920244	0.0
4	0.0	0.0	0.0	0.7917019224510807	0.0
5	0.0	0.0	0.0	2.327177624065845	0.0
6	0.0	0.0	0.0	2.5668183405028313	0.0
7	0.0	0.0	0.0	3.0922528002461496	0.0
8	0.0	0.0	0.0	3.827742702792258	0.0
9	0.0	0.0	0.0	4.007029461015485	0.0
10	0.0	0.0	0.0	6.501068028378194	0.0
11	0.0	0.0	0.0	7.0176269060312535	0.0
12	0.0	0.0	0.0	9.088004354952279	0.0
13	0.0	0.0	0.0	9.326461660443897	0.0
14	0.0	0.0	0.0	9.46432903557925	0.0
15	0.0	0.0	0.0	9.774974408738306	0.0
16	0.0	0.0	0.0	10.080294432643207	0.0
17	0.0	0.0	0.0	10.407507559037414	0.0
18	0.0	0.0	0.0	10.74832991130335	0.0
19	0.0	0.0	0.0	10.9856038058496	0.0
20	0.0	0.0	0.0	11.195067543179707	0.0
21	0.0	0.0	0.0	11.454234540215262	0.0
22	0.0	0.0	0.0	11.729969290485968	0.0
23	0.0	0.0	0.0	11.978485589013212	0.0
24	0.0	0.0	0.0	12.200966846742366	0.0
25	0.0	0.0	0.0	12.396821358200741	0.0
26	0.0	0.0	0.0	12.602143157222061	0.0
27	0.0	0.0	0.0	12.808648367188749	0.0
28	0.0	0.0	0.0	13.026987686609113	0.0
29	0.0	0.0	0.0	13.23940995130264	0.0
30	0.0	0.0	0.0	13.490884777193305	0.0
31	0.0	0.0	0.0	13.709224096613669	0.0
32	0.0	0.0	0.0	13.936438998124293	0.0
33	0.0	0.0	0.0	14.172529481725176	0.0
34	0.0	0.0	0.0	14.394419033981645	0.0
35	0.0	0.0	0.0	14.661278202162093	0.0
36	0.0	0.0	0.0	14.918078377306912	0.0
37	0.0	0.0	0.0	15.18079560717857	0.0
38	0.0	0.0	0.0	15.408010508689195	0.0
39	0.0	0.0	0.0	15.647059519653498	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	35	1.2037162E-7	45.000004	4
ATATGCA	35	1.2037162E-7	45.000004	25
TACGGGG	35	1.2037162E-7	45.000004	4
GGTACCC	20	7.016201E-4	45.0	8
GTACCCA	20	7.016201E-4	45.0	9
CGCGGCG	20	7.016201E-4	45.0	21
GGTCAGA	20	7.016201E-4	45.0	8
TACGGGT	20	7.016201E-4	45.0	4
GCTCTAT	20	7.016201E-4	45.0	18
CTACGAA	20	7.016201E-4	45.0	11
GGAACGA	20	7.016201E-4	45.0	9
GCTACGA	20	7.016201E-4	45.0	10
ATACGTC	20	7.016201E-4	45.0	6
ATTGCGG	20	7.016201E-4	45.0	2
AGCTACG	20	7.016201E-4	45.0	9
GACCTAT	20	7.016201E-4	45.0	33
AATACGT	20	7.016201E-4	45.0	5
CTAGCGG	20	7.016201E-4	45.0	2
TCGCAGG	25	3.8766506E-5	44.999996	3
CATAGGA	25	3.8766506E-5	44.999996	4
>>END_MODULE