##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936185.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452572
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.749381313912483	31.0	31.0	34.0	30.0	34.0
2	31.92952281625907	33.0	31.0	34.0	30.0	34.0
3	31.078257161291464	31.0	31.0	34.0	27.0	34.0
4	35.273324465499414	37.0	35.0	37.0	33.0	37.0
5	31.80951981121236	37.0	35.0	37.0	0.0	37.0
6	33.792044138833155	37.0	35.0	37.0	17.0	37.0
7	21.12369744482646	32.0	0.0	37.0	0.0	37.0
8	28.275730712461222	35.0	17.0	37.0	17.0	37.0
9	34.89359262172649	37.0	32.0	39.0	32.0	39.0
10	36.38672741574821	37.0	35.0	39.0	32.0	39.0
11	36.82582439921162	39.0	37.0	39.0	33.0	39.0
12	37.169495682454944	39.0	37.0	39.0	34.0	39.0
13	36.99975252556499	39.0	37.0	39.0	33.0	39.0
14	38.41251999681818	40.0	38.0	41.0	34.0	41.0
15	38.55341470528446	40.0	38.0	41.0	34.0	41.0
16	38.61671954959653	40.0	38.0	41.0	34.0	41.0
17	38.568581352801324	40.0	38.0	41.0	34.0	41.0
18	38.3413976118717	40.0	38.0	41.0	34.0	41.0
19	38.132772243974436	40.0	37.0	41.0	34.0	41.0
20	37.94637317377124	40.0	36.0	41.0	34.0	41.0
21	37.82018330784936	40.0	36.0	41.0	33.0	41.0
22	37.697272478191316	39.0	35.0	41.0	33.0	41.0
23	37.558772968721	39.0	35.0	41.0	33.0	41.0
24	37.33245759790707	39.0	35.0	41.0	33.0	41.0
25	37.18148051580743	39.0	35.0	41.0	33.0	41.0
26	37.05065934260184	39.0	35.0	40.0	33.0	41.0
27	36.973297066544106	39.0	35.0	40.0	33.0	41.0
28	36.87826688350141	39.0	35.0	40.0	33.0	41.0
29	36.81184209363372	39.0	35.0	40.0	33.0	41.0
30	36.60423534818769	38.0	35.0	40.0	32.0	41.0
31	36.37161379846743	38.0	35.0	40.0	31.0	41.0
32	36.05319595556066	38.0	35.0	40.0	30.0	41.0
33	35.59674261774922	38.0	35.0	40.0	26.0	41.0
34	35.06348824054515	38.0	34.0	40.0	23.0	41.0
35	34.57039321920048	38.0	34.0	40.0	20.0	41.0
36	34.19808560847777	38.0	33.0	40.0	18.0	41.0
37	33.993373430084056	38.0	33.0	40.0	15.0	41.0
38	33.787576341444016	38.0	33.0	40.0	15.0	41.0
39	33.644823365122015	38.0	33.0	40.0	15.0	41.0
40	33.48798865152948	38.0	33.0	40.0	12.0	41.0
41	33.33478650910794	38.0	33.0	40.0	12.0	41.0
42	33.209347904863755	38.0	33.0	40.0	10.0	41.0
43	33.11168609635594	38.0	33.0	40.0	10.0	41.0
44	32.94341894770335	37.0	32.0	40.0	10.0	41.0
45	32.86608097717048	37.0	32.0	40.0	10.0	41.0
46	32.756723792015414	37.0	32.0	40.0	10.0	41.0
47	32.69406856809524	37.0	31.0	40.0	10.0	41.0
48	32.59971893974881	37.0	31.0	40.0	10.0	41.0
49	32.475933111195566	36.0	31.0	40.0	10.0	41.0
50	32.34457500684974	36.0	31.0	40.0	10.0	41.0
51	30.768646756759146	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	27.0
10	32.0
11	20.0
12	14.0
13	16.0
14	17.0
15	24.0
16	54.0
17	104.0
18	218.0
19	428.0
20	775.0
21	1354.0
22	2501.0
23	4538.0
24	7329.0
25	10899.0
26	13352.0
27	13436.0
28	12273.0
29	10950.0
30	11183.0
31	12621.0
32	15763.0
33	22491.0
34	29688.0
35	38196.0
36	46637.0
37	75481.0
38	74290.0
39	47847.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.52157888689534	18.338739471288545	21.451393369452816	25.68828827236329
2	43.349345518503135	20.685106458198916	21.777529321301362	14.188018701996588
3	28.051890085997368	21.363009642664593	36.67946757642982	13.905632694908213
4	23.379263410020947	22.188734610183573	38.18154901319569	16.25045296659979
5	19.35139602096462	33.59487551152082	32.58796390408598	14.465764563428582
6	22.10388623246688	29.608327514737987	36.510433698947345	11.777352553847784
7	40.93094579426036	41.80771236399954	14.803390399759596	2.4579514419805024
8	71.10890642814844	4.80741185932846	21.355717985204564	2.727963727318526
9	65.91304808958574	6.367826555774552	22.892931953368745	4.826193401270959
10	34.5061117347074	24.33226094411497	29.31378874521623	11.847838575961395
11	26.1198218184068	23.259945378856845	35.73685513023342	14.88337767250294
12	23.10085467063804	21.458464067595877	39.32412964125046	16.116551620515633
13	21.221816639120405	21.620206287618323	40.62226562845249	16.535711444808783
14	18.633278240810302	23.37373942709668	40.457429977992454	17.535552354100563
15	18.025861078458234	22.770299532450085	42.11042662824921	17.093412760842476
16	21.463546131886197	22.256348161176565	39.83233607028274	16.4477696366545
17	22.174151295263517	21.787914409198976	38.92706574865436	17.110868546883147
18	22.7199208081808	21.861935780384115	38.90894708466277	16.509196326772315
19	21.92645590093952	23.44312065262544	37.62539441238079	17.00502903405425
20	22.948834660562298	23.259945378856845	37.51690338774824	16.27431657283261
21	22.44438453991851	23.263922646562314	38.863208506049865	15.42848430746931
22	22.41698558461416	20.89678548385671	39.17365634639351	17.512572585135626
23	20.617050988571982	22.856694625385572	39.49139584419717	17.034858541845274
24	20.09801755300814	22.23137975835889	40.091963267723145	17.578639420909823
25	20.55982252547661	23.2685627922187	38.36516620559823	17.806448476706468
26	20.29091503672344	23.212439125708176	37.965450801198486	18.531195036369905
27	19.804583580071235	23.311870818344925	38.89878295608213	17.98476264550171
28	18.491201399998232	24.20255782505325	39.318384699009215	17.9878560759393
29	19.494135739727604	23.533713972583367	39.04196459347905	17.93018569420998
30	20.695712505413503	22.678380456590332	39.095657707502895	17.53024933049327
31	21.558337678866568	22.980210883572116	38.26153628593904	17.19991515162228
32	21.62352067737288	23.282704188504812	37.901593558593994	17.192181575528316
33	20.91932333418771	23.673139301591792	36.955445763326054	18.452091600894445
34	20.090946854865084	24.43434414855537	37.95263516081419	17.522073835765358
35	20.18507552389454	25.405018428007036	36.15049097160231	18.259415076496115
36	21.08194939147804	27.047850949683145	34.006522719036965	17.86367693980184
37	20.607991656576193	27.074587027036586	33.65166205598225	18.665759260404975
38	20.95953792987635	27.33001599745455	32.97442174946749	18.73602432320161
39	21.232643645651965	26.54737809674483	32.88714281926411	19.332835438339092
40	22.34649956250055	25.685857719876616	33.202893683215045	18.764749034407785
41	20.226173956851063	26.451039834545664	33.29017261341842	20.032613595184852
42	21.153982128810444	26.025030271426424	33.190519961464695	19.630467638298438
43	21.154865966078326	26.41502346587946	32.44677090054179	19.98333966750042
44	20.70676047126203	26.893400387120725	32.56874044350954	19.831098698107706
45	20.724437216619677	26.242012320691515	32.42666360269747	20.606886859991338
46	20.772385388402288	26.51246652466348	32.45914462229214	20.256003464642088
47	20.261527447566355	26.77319851868874	33.632217636088846	19.333056397656065
48	20.946059411541146	26.383647242869646	33.31867636530762	19.35161698028159
49	20.89170341956639	26.074525158427832	33.97492553671018	19.0588458852956
50	19.656761797017932	26.45457518361719	33.91416172454328	19.974501294821597
51	20.062222143658907	25.95631192384858	33.20797574750537	20.77349018498714
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	805.0
1	1058.0
2	1311.0
3	14162.5
4	27014.0
5	18186.0
6	9358.0
7	8057.0
8	6756.0
9	6465.0
10	6174.0
11	5961.5
12	5749.0
13	5490.0
14	5231.0
15	4949.0
16	4667.0
17	4426.5
18	4186.0
19	3919.0
20	3652.0
21	3706.0
22	3760.0
23	3919.0
24	4078.0
25	4278.0
26	5165.0
27	5852.0
28	6598.5
29	7345.0
30	8117.0
31	8889.0
32	10476.5
33	12064.0
34	13372.5
35	14681.0
36	15406.0
37	16131.0
38	16858.5
39	17586.0
40	18781.0
41	19976.0
42	21282.0
43	22588.0
44	23445.5
45	24303.0
46	25855.0
47	27407.0
48	26951.0
49	26495.0
50	25974.5
51	25454.0
52	24487.0
53	23520.0
54	22771.5
55	22023.0
56	20793.0
57	19563.0
58	18524.0
59	17485.0
60	16517.0
61	15549.0
62	13824.0
63	12099.0
64	10476.5
65	8854.0
66	7493.0
67	6132.0
68	5170.5
69	4209.0
70	3530.5
71	2852.0
72	2348.0
73	1844.0
74	1453.0
75	846.5
76	631.0
77	480.5
78	330.0
79	276.0
80	222.0
81	168.0
82	114.0
83	77.0
84	40.0
85	30.0
86	20.0
87	17.5
88	15.0
89	14.0
90	13.0
91	8.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	452572.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.07118979567972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.60704508466641	33.911641443948206
2	8.82754444301333	8.1339095092773
3	4.4401156193917295	6.136842282472752
4	3.0063626715101415	5.540268209351618
5	2.1119536540585	4.865010881790423
6	1.6955145225015764	4.686862282050084
7	1.3209566547205016	4.260063132604609
8	1.0997804294491962	4.053455431898246
9	0.8439092340139689	3.499191224152583
>10	3.0083636955392903	18.90626066797438
>50	0.026310625513672966	0.8277666593840743
>100	0.00961349778383395	0.8555065164566592
>500	0.0010119471351404158	0.3517600363305446
>1k	5.059735675702079E-4	0.47833770347930915
>5k	0.0010119471351404158	3.4931240188291657
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9491	2.097124877367579	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5494	1.2139504874362532	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2052	0.45340851842358787	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	905	0.19996818185835627	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	604	0.13345942745021788	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTC	463	0.10230416375736899	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016571948772791955	0.0
2	0.0	0.0	0.0	0.08065015069425417	0.0
3	0.0	0.0	0.0	0.12152762433380766	0.0
4	0.0	0.0	0.0	0.1957699548359156	0.0
5	0.0	0.0	0.0	0.4085537770785643	0.0
6	0.0	0.0	0.0	0.5570384380827802	0.0
7	0.0	0.0	0.0	0.7112680413282306	0.0
8	0.0	0.0	0.0	1.055080738534421	0.0
9	0.0	0.0	0.0	1.2442219138612198	0.0
10	0.0	0.0	0.0	1.6417277250912563	0.0
11	0.0	0.0	0.0	1.867990065669109	0.0
12	0.0	0.0	0.0	2.1455149677841314	0.0
13	0.0	0.0	0.0	2.2487029688093827	0.0
14	0.0	0.0	0.0	2.3070362284896104	0.0
15	0.0	0.0	0.0	2.3711144304110725	0.0
16	0.0	0.0	0.0	2.4508807438374447	0.0
17	0.0	0.0	0.0	2.5496495585232846	0.0
18	0.0	0.0	0.0	2.667199915151622	0.0
19	0.0	0.0	0.0	2.737023059314319	0.0
20	0.0	0.0	0.0	2.8101605932315743	0.0
21	0.0	0.0	0.0	2.895671848899181	0.0
22	0.0	0.0	0.0	3.000406565143226	0.0
23	0.0	0.0	0.0	3.0974077052933016	0.0
24	0.0	0.0	0.0	3.1796045712063497	0.0
25	0.0	0.0	0.0	3.2668835014097204	0.0
26	0.0	0.0	0.0	3.3420096691797108	0.0
27	0.0	0.0	0.0	3.4319401111867283	0.0
28	0.0	0.0	0.0	3.517451366854335	0.0
29	0.0	0.0	0.0	3.6148944256383513	0.0
30	0.0	0.0	0.0	3.7350962940703356	2.2095931697055937E-4
31	0.0	0.0	0.0	3.8389471730464986	2.2095931697055937E-4
32	0.0	0.0	0.0	3.95008970948269	2.2095931697055937E-4
33	0.0	0.0	0.0	4.054382507092794	2.2095931697055937E-4
34	0.0	0.0	0.0	4.174142456890837	2.2095931697055937E-4
35	0.0	0.0	0.0	4.296774877809498	2.2095931697055937E-4
36	2.2095931697055937E-4	0.0	0.0	4.416976746241482	2.2095931697055937E-4
37	2.2095931697055937E-4	0.0	0.0	4.5475637025710824	2.2095931697055937E-4
38	2.2095931697055937E-4	0.0	0.0	4.698478916061974	2.2095931697055937E-4
39	2.2095931697055937E-4	0.0	0.0	4.9112627383046235	2.2095931697055937E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGA	25	3.887044E-5	45.000004	29
GTCCGAC	25	3.887044E-5	45.000004	26
TAATCGT	20	7.0287473E-4	45.000004	21
CGTTTTA	2045	0.0	44.449883	1
CGTTATT	975	0.0	43.846153	1
CGTTTTT	4015	0.0	43.038605	1
GTTTTAT	2275	0.0	41.142857	2
GTTATTT	1070	0.0	40.58411	2
ACACGAC	50	1.0786607E-9	40.500004	26
CGGTTTT	325	0.0	38.076923	1
AAGGCGA	215	0.0	37.67442	6
CGTTGAT	30	1.1388287E-4	37.499996	25
TATGCGG	30	1.1388287E-4	37.499996	2
TGGCACG	30	1.1388287E-4	37.499996	1
GCGATAT	30	1.1388287E-4	37.499996	9
AGTACGG	30	1.1388287E-4	37.499996	2
AGGCGAT	325	0.0	37.384617	7
CGGTTTA	115	0.0	37.173916	1
CATACGA	85	0.0	37.058823	18
TTAATCG	25	0.0021057616	36.000004	20
>>END_MODULE