##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936184.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45514
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.269565408445754	31.0	31.0	33.0	28.0	34.0
2	31.413059717889002	31.0	31.0	34.0	30.0	34.0
3	30.707584479500813	31.0	30.0	34.0	26.0	34.0
4	34.900294414905304	35.0	35.0	37.0	32.0	37.0
5	31.61405281891286	35.0	35.0	37.0	10.0	37.0
6	33.53317660500066	35.0	35.0	37.0	19.0	37.0
7	21.18906270598058	32.0	0.0	37.0	0.0	37.0
8	28.115019554422815	35.0	17.0	37.0	17.0	37.0
9	34.258469921342886	35.0	32.0	39.0	28.0	39.0
10	35.8345564002285	37.0	35.0	39.0	32.0	39.0
11	36.3596475809641	37.0	35.0	39.0	32.0	39.0
12	36.73728962517028	39.0	35.0	39.0	33.0	39.0
13	36.455310453926266	39.0	35.0	39.0	32.0	39.0
14	37.63683701718153	39.0	37.0	41.0	33.0	41.0
15	37.77868348200553	40.0	37.0	41.0	33.0	41.0
16	37.87834512457705	40.0	37.0	41.0	33.0	41.0
17	37.80177527793646	40.0	37.0	41.0	33.0	41.0
18	37.63696884475107	39.0	36.0	40.0	33.0	41.0
19	37.47394208375445	39.0	36.0	40.0	32.0	41.0
20	37.26422639187942	39.0	35.0	40.0	32.0	41.0
21	37.20143252625566	39.0	35.0	40.0	32.0	41.0
22	37.14386782089028	39.0	35.0	40.0	32.0	41.0
23	37.06477127916685	38.0	35.0	40.0	33.0	41.0
24	36.81570505778442	38.0	35.0	40.0	32.0	41.0
25	36.66750889836094	38.0	35.0	40.0	32.0	41.0
26	36.49850595421189	38.0	35.0	40.0	31.0	41.0
27	36.433756646306634	38.0	35.0	40.0	31.0	41.0
28	36.39253855956409	38.0	35.0	40.0	31.0	41.0
29	36.24355143472338	38.0	35.0	40.0	31.0	41.0
30	35.96910840620468	38.0	34.0	40.0	30.0	41.0
31	35.7882849233203	38.0	34.0	40.0	30.0	41.0
32	35.48426857670167	38.0	34.0	40.0	28.0	41.0
33	35.10827437711473	38.0	34.0	40.0	25.0	41.0
34	34.76462187458804	38.0	33.0	40.0	24.0	41.0
35	34.13958342488026	37.0	33.0	40.0	21.0	41.0
36	33.733378740607286	37.0	33.0	40.0	18.0	41.0
37	33.47271169310542	37.0	33.0	40.0	18.0	41.0
38	33.124577053214395	37.0	32.0	40.0	15.0	41.0
39	33.019949905523575	37.0	32.0	40.0	15.0	41.0
40	32.84268576701674	37.0	31.0	40.0	15.0	41.0
41	32.571340686382214	36.0	31.0	40.0	13.0	41.0
42	32.42549545194885	36.0	31.0	40.0	12.0	41.0
43	32.223579557938216	36.0	30.0	40.0	12.0	41.0
44	32.07854726018368	36.0	30.0	40.0	10.0	41.0
45	31.94617040910489	35.0	30.0	40.0	10.0	41.0
46	31.633651184251	35.0	29.0	40.0	10.0	41.0
47	31.493298765215098	35.0	28.0	39.0	10.0	41.0
48	31.300918398734456	35.0	28.0	39.0	10.0	41.0
49	31.109834336687612	35.0	27.0	39.0	8.0	41.0
50	30.853297886364636	35.0	27.0	39.0	8.0	41.0
51	28.50384497077822	33.0	21.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	1.0
12	2.0
13	0.0
14	1.0
15	2.0
16	10.0
17	14.0
18	17.0
19	41.0
20	106.0
21	178.0
22	269.0
23	499.0
24	722.0
25	1077.0
26	1366.0
27	1495.0
28	1424.0
29	1429.0
30	1594.0
31	1913.0
32	2386.0
33	2959.0
34	3737.0
35	4501.0
36	4630.0
37	6504.0
38	5463.0
39	3170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.43054884211451	18.44487410467109	21.463725447115173	23.66085160609922
2	39.72404095443161	27.606890187634576	18.486619501691788	14.182449356242035
3	25.730544447862197	26.706068462451114	33.67535263874852	13.888034450938171
4	24.111262468690953	20.34978248451026	39.91079667794525	15.62815836885354
5	21.219844443467945	30.660895548622403	30.98167596783407	17.13758404007558
6	19.620336599727555	32.04069077646439	36.90732521861405	11.431647405194006
7	37.28742804411829	41.71463725447116	17.57700927187239	3.4209254295381646
8	59.656369468734894	9.66515797337083	24.878059498176384	5.800413059717889
9	53.84497077822209	8.891769565408445	25.638265149184864	11.624994507184603
10	32.06485916421321	21.06165136002109	33.02720042184822	13.846289053917477
11	23.531221162719163	21.518653601089774	37.05672979742497	17.893395438766095
12	22.333787406072858	18.473436744737885	41.88381596871292	17.308959880476337
13	18.649206837456607	20.91444390736916	45.80129191018148	14.635057344992749
14	15.393065869842246	25.038449707782224	41.74759414685591	17.82089027551962
15	14.186843608560004	22.656764951443513	47.717185920815574	15.439205519180913
16	16.983785208946696	23.65426022762227	40.42272707298853	18.9392274904425
17	16.313661730456563	22.61941380674078	39.59880476336951	21.468119699433142
18	17.475941468559125	23.78608779716131	41.286197653469266	17.4517730808103
19	16.900294414905304	25.389989893219667	37.77738717757174	19.93232851430329
20	16.304873225820625	26.793953508810475	40.299688008085425	16.601485257283475
21	16.768466845366262	26.895021312123742	40.72593048292833	15.61058135958167
22	14.839390077778267	22.95996836138331	39.22089906402426	22.97974249681417
23	14.437315990684185	26.088676011776595	40.93026321571385	18.54374478182537
24	18.152656325526213	23.766313661730457	37.94876301797249	20.132266994770838
25	16.55095135562684	27.50142813200334	35.96915234872786	19.978468163641956
26	15.27661818341609	24.069517071670255	38.326668717317744	22.327196027595907
27	17.548446631805596	24.37711473392802	37.53350617392451	20.540932460341875
28	13.554071274772598	23.232412005097334	40.01625873357648	23.197257986553588
29	20.132266994770838	22.397504064683392	36.88095970470624	20.58926923583952
30	17.370479412927892	23.52243265808323	38.21241815705058	20.894669771938304
31	18.227358614931667	22.80177527793646	36.303115524893435	22.667750582238433
32	19.473129147075625	22.667750582238433	37.21492288087182	20.644197389814124
33	16.082963483763237	21.988838599112363	37.9619457749264	23.966252142198005
34	17.746187986114162	21.11438238783671	40.50841499318891	20.63101463286022
35	15.896207760249592	22.371138550775584	36.12075405369776	25.61189963527706
36	18.387748824537507	25.376807136265768	34.795886979830385	21.439557059366347
37	14.980006151953246	23.57296655973986	38.92428703256141	22.522740255745486
38	15.329349211231708	25.475677813420045	35.63958342488026	23.555389550467986
39	17.62534604737004	22.98633387529112	39.04293184514655	20.345388232192292
40	18.310849408973063	21.485696708705014	39.00997495276179	21.193478929560136
41	15.795139956936326	26.209517950520716	34.96506569407216	23.0302763984708
42	17.453970206969284	23.825636068023027	36.67442984576174	22.045963879245946
43	18.991958518258116	24.13762798259876	34.73217032121985	22.138243177923275
44	18.57450454805115	22.990728127609085	34.894757656984666	23.5400096673551
45	18.18781034406996	21.518653601089774	34.69921342883509	25.594322626005184
46	21.50547084413587	23.59933207364767	33.152436612910314	21.74276046930615
47	15.40624862679615	23.65865447994024	39.497736960056244	21.437359933207365
48	17.02113635364943	24.686909522344774	34.354264621874584	23.937689502131214
49	17.124401283121678	22.221733971964667	38.210221030891596	22.44364371402206
50	16.79483235927407	20.778222085512148	37.966340027244364	24.460605527969417
51	16.5839082480116	21.063848486180074	34.3806301357824	27.971613130025926
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	228.0
1	232.5
2	237.0
3	1572.5
4	2908.0
5	1872.0
6	836.0
7	682.5
8	529.0
9	529.0
10	529.0
11	520.0
12	511.0
13	471.5
14	432.0
15	405.5
16	379.0
17	370.0
18	361.0
19	357.5
20	354.0
21	364.5
22	375.0
23	395.0
24	415.0
25	462.5
26	565.5
27	621.0
28	684.0
29	747.0
30	796.5
31	846.0
32	921.0
33	996.0
34	1100.5
35	1205.0
36	1210.5
37	1216.0
38	1278.5
39	1341.0
40	1551.0
41	1761.0
42	2034.5
43	2308.0
44	2860.0
45	3412.0
46	4443.5
47	5475.0
48	4850.0
49	4225.0
50	3913.0
51	3601.0
52	3010.5
53	2420.0
54	2073.0
55	1726.0
56	1543.5
57	1361.0
58	1210.5
59	1060.0
60	932.5
61	805.0
62	715.5
63	626.0
64	505.0
65	384.0
66	339.0
67	294.0
68	245.5
69	197.0
70	164.5
71	132.0
72	103.0
73	74.0
74	55.0
75	29.5
76	23.0
77	15.5
78	8.0
79	5.5
80	3.0
81	3.0
82	3.0
83	2.5
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	45514.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.69380849848397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.23231269192928	65.02175154897394
2	7.537044214543395	11.108669859823351
3	2.1853850511314508	4.831480423605924
4	0.9808890611490415	2.8914180252230084
5	0.369696789004502	1.3622182185701102
6	0.24745833457559407	1.0941688271740564
7	0.10136847440446022	0.5229160258382036
8	0.04472138576667362	0.26365513907808585
9	0.04173996004889538	0.27683789603199016
>10	0.20273694880892043	2.8892208990640245
>50	0.023851405742225933	1.118337214922881
>100	0.026832831460004175	4.6666959616821195
>500	0.0029814257177782416	1.4303291294986158
>1k	0.0029814257177782416	2.5223008305136885
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1148	2.5223008305136885	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	651	1.4303291294986158	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC	318	0.6986861185569275	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG	303	0.6657292261721668	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	288	0.632772333787406	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC	287	0.6305752076284221	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG	231	0.5075361427253152	No Hit
GAATCTATCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC	202	0.44381948411477784	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	198	0.4350309794788417	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC	180	0.3954827086171288	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	117	0.25706376060113373	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	90	0.1977413543085644	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT	71	0.15599595728786747	No Hit
GAATATGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC	66	0.14501032649294723	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	60	0.13182756953904293	No Hit
GAATGAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTC	59	0.12963044338005889	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG	57	0.1252361910620908	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT	54	0.11864481258513865	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTC	52	0.11425056026717054	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTT	48	0.10546205563123434	No Hit
CCTGTCACTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	48	0.10546205563123434	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07030803708748956	0.0
2	0.0	0.0	0.0	0.6173924506745178	0.0
3	0.0	0.0	0.0	0.8283165619369864	0.0
4	0.0	0.0	0.0	1.2347849013490355	0.0
5	0.0	0.0	0.0	3.108933514962429	0.0
6	0.0	0.0	0.0	3.8317880212681814	0.0
7	0.0	0.0	0.0	4.756778134200466	0.0
8	0.0	0.0	0.0	6.608955486224019	0.0
9	0.0	0.0	0.0	7.19558817067276	0.0
10	0.0	0.0	0.0	10.049655051193039	0.0
11	0.0	0.0	0.0	12.178670299248584	0.0
12	0.0	0.0	0.0	14.839390077778265	0.0
13	0.0	0.0	0.0	15.55785033176605	0.0
14	0.0	0.0	0.0	15.900602012567562	0.0
15	0.0	0.0	0.0	16.603682383442457	0.0
16	0.0	0.0	0.0	17.515489739420836	0.0
17	0.0	0.0	0.0	18.44047985235312	0.0
18	0.0	0.0	0.0	19.56760557191194	0.0
19	0.0	0.0	0.0	20.23553192424309	0.0
20	0.0	0.0	0.0	20.93421804280002	0.0
21	0.0	0.0	0.0	21.670255306059673	0.0
22	0.0	0.0	0.0	22.43485520938612	0.0
23	0.0	0.0	0.0	23.24779188821022	0.0
24	0.0	0.0	0.0	23.76851078788944	0.0
25	0.0	0.0	0.0	24.309003822999518	0.0
26	0.0	0.0	0.0	24.840708353473655	0.0
27	0.0	0.0	0.0	25.405369776332556	0.0
28	0.0	0.0	0.0	25.937074306806696	0.0
29	0.0	0.0	0.0	26.532495495891375	0.0
30	0.0	0.0	0.0	27.1762534604737	0.0
31	0.0	0.0	0.0	27.758491892604475	0.0
32	0.0	0.0	0.0	28.301182053873532	0.0
33	0.0	0.0	0.0	28.79333831348596	0.0
34	0.0	0.0	0.0	29.292085951575338	0.0
35	0.0	0.0	0.0	29.889704266819002	0.0
36	0.0	0.0	0.0	30.381860526431428	0.0
37	0.0	0.0	0.0	30.81030012743332	0.0
38	0.0	0.0	0.0	31.42988970426682	0.0
39	0.0	0.0	0.0	32.165926967526474	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAC	20	6.962015E-4	45.000004	5
CGTTATT	40	6.5701897E-9	45.000004	1
AGCTCGC	20	6.962015E-4	45.000004	19
AATTCGA	20	6.962015E-4	45.000004	13
GTATCTT	20	6.962015E-4	45.000004	5
TTCGAGC	20	6.962015E-4	45.000004	15
GAGCTCG	20	6.962015E-4	45.000004	18
GGCGACT	20	6.962015E-4	45.000004	10
CTAGGGA	20	6.962015E-4	45.000004	5
GCTCGCA	20	6.962015E-4	45.000004	20
GTAAGGG	20	6.962015E-4	45.000004	3
CGAGCTC	20	6.962015E-4	45.000004	17
GAATTCG	20	6.962015E-4	45.000004	12
CGCATTT	20	6.962015E-4	45.000004	23
AGTCTCT	20	6.962015E-4	45.000004	7
GCATTTT	20	6.962015E-4	45.000004	24
ACGGCGC	20	6.962015E-4	45.000004	8
CTCGCAT	20	6.962015E-4	45.000004	21
CGACTGT	20	6.962015E-4	45.000004	12
TCGAGCT	20	6.962015E-4	45.000004	16
>>END_MODULE