##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936183.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147530 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.341157730631057 31.0 31.0 34.0 28.0 34.0 2 31.50467701484444 31.0 31.0 34.0 30.0 34.0 3 30.56766081474954 31.0 30.0 34.0 26.0 34.0 4 34.8698637565241 35.0 35.0 37.0 32.0 37.0 5 31.501050633769403 35.0 35.0 37.0 0.0 37.0 6 33.515935741883006 35.0 35.0 37.0 17.0 37.0 7 21.319067308344067 32.0 0.0 37.0 0.0 37.0 8 28.320199281502067 35.0 17.0 37.0 17.0 37.0 9 34.61629499084932 37.0 32.0 39.0 28.0 39.0 10 36.0340608689758 37.0 35.0 39.0 32.0 39.0 11 36.54911543414899 37.0 35.0 39.0 32.0 39.0 12 37.03505049820375 39.0 37.0 39.0 34.0 39.0 13 36.949630583610116 39.0 37.0 39.0 33.0 39.0 14 38.36714566528842 40.0 38.0 41.0 34.0 41.0 15 38.45879482139226 40.0 38.0 41.0 34.0 41.0 16 38.447671660001355 40.0 38.0 41.0 34.0 41.0 17 38.39989154748187 40.0 38.0 41.0 34.0 41.0 18 38.17954992204975 40.0 38.0 41.0 34.0 41.0 19 37.96427167355792 39.0 37.0 41.0 34.0 41.0 20 37.75134548905307 39.0 36.0 40.0 34.0 41.0 21 37.62970921168576 39.0 35.0 40.0 33.0 41.0 22 37.50611401070969 39.0 35.0 40.0 33.0 41.0 23 37.35468040398563 39.0 35.0 40.0 33.0 41.0 24 37.09265234189656 39.0 35.0 40.0 33.0 41.0 25 36.957540839151356 38.0 35.0 40.0 32.0 41.0 26 36.77308344065614 38.0 35.0 40.0 32.0 41.0 27 36.71595607673016 38.0 35.0 40.0 32.0 41.0 28 36.570148444384195 38.0 35.0 40.0 31.0 41.0 29 36.54195756795228 38.0 35.0 40.0 31.0 41.0 30 36.37490002033485 38.0 35.0 40.0 31.0 41.0 31 36.11847081949434 38.0 35.0 40.0 30.0 41.0 32 35.88453195960144 38.0 34.0 40.0 30.0 41.0 33 35.415468040398565 38.0 34.0 40.0 25.0 41.0 34 34.849434013420996 38.0 33.0 40.0 24.0 41.0 35 34.25005761540026 38.0 33.0 40.0 20.0 41.0 36 33.70664949501796 38.0 33.0 40.0 16.0 41.0 37 33.435457195146746 38.0 33.0 40.0 15.0 41.0 38 33.13128177319867 38.0 32.0 40.0 12.0 41.0 39 32.88114281840981 38.0 31.0 40.0 10.0 41.0 40 32.67146343116654 37.0 31.0 40.0 10.0 41.0 41 32.3679590591744 37.0 30.0 40.0 10.0 41.0 42 32.17452721480377 37.0 30.0 40.0 10.0 41.0 43 31.90122686911137 37.0 30.0 40.0 9.0 41.0 44 31.604961702704536 37.0 29.0 40.0 9.0 41.0 45 31.511001152308005 36.0 28.0 40.0 8.0 41.0 46 31.207401884362504 36.0 26.0 40.0 8.0 41.0 47 31.076031993492848 36.0 25.0 40.0 8.0 41.0 48 30.83720599200163 36.0 25.0 40.0 8.0 41.0 49 30.589358096658305 35.0 24.0 40.0 8.0 41.0 50 30.328950044058836 35.0 24.0 40.0 8.0 41.0 51 28.301070968616553 34.0 19.0 38.0 7.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 6.0 10 2.0 11 3.0 12 2.0 13 1.0 14 0.0 15 3.0 16 6.0 17 26.0 18 54.0 19 130.0 20 258.0 21 462.0 22 914.0 23 1560.0 24 2701.0 25 3960.0 26 5173.0 27 5337.0 28 4971.0 29 4733.0 30 4901.0 31 5385.0 32 6346.0 33 7998.0 34 9864.0 35 12766.0 36 14960.0 37 21559.0 38 20462.0 39 12982.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.391581373280008 18.76092998034298 21.529180505659866 28.318308140717143 2 42.29173727377483 22.928217989561446 20.82288348132583 13.957161255337896 3 25.02541855893717 22.817731986714566 38.52097878397614 13.635870670372126 4 22.58727038568427 21.246526130278585 41.16722022639463 14.998983257642514 5 17.952280892021964 32.34325221988748 34.36521385480919 15.339253033281366 6 19.205585304683794 29.219141869450283 39.1696604080526 12.405612417813327 7 37.611333288144785 41.0553785670711 17.717752321561715 3.6155358232223955 8 62.82315461262116 5.978445062021284 25.75747305632753 5.440927269030028 9 57.22429336406155 7.673693486070629 27.634379448247813 7.467633701620009 10 26.722022639463162 25.409069341828783 35.078966989764794 12.789941028943266 11 18.564359791228902 23.304412661831492 41.198400325357554 16.932827221582052 12 17.42764183555887 20.40127431708805 44.786145190808654 17.38493865654443 13 16.31803700942181 21.075035585982512 46.60001355656477 16.00691384803091 14 14.445875415169795 24.882396800650717 42.943808039042906 17.72791974513658 15 13.817528638243068 24.07171422761472 46.67660814749542 15.434148986646784 16 15.320273842608284 24.670236562055177 42.91127228360334 17.098217311733208 17 14.80309089676676 24.778011251948755 41.958923608757544 18.459974242526943 18 15.373144445197587 25.335863892089744 42.54727851962313 16.743713143089543 19 15.134548905307396 26.429200840507015 40.44397749610249 17.992272758083104 20 15.798142750626992 26.38853114620755 40.97268352199553 16.84064258116993 21 15.774418762285636 26.576967396461736 41.516979597370025 16.1316342438826 22 15.303328136650174 24.31098759574324 39.63668406425811 20.749000203348473 23 14.224903409476038 25.73035992679455 41.593574188300686 18.451162475428724 24 16.35328407781468 23.55588693824985 41.55019318104792 18.540635802887547 25 15.893038703992406 26.349217108384735 39.34589575001694 18.41184843760591 26 14.648546058428794 25.077611333288147 41.37938046499017 18.89446214329289 27 16.44682437470345 25.176574256083505 39.563478614519084 18.81312275469396 28 13.982918728394225 25.577848573171558 40.89066630515828 19.548566393275944 29 15.363654849861044 23.018369145258593 40.76526808106826 20.852707923812105 30 14.830881854538061 23.889378431505456 40.42838744662103 20.851352267335457 31 14.911543414898665 25.19216430556497 39.553311190944214 20.34298108859215 32 14.693282722158205 24.847827560496167 38.598251203145125 21.8606385142005 33 13.706364807157867 23.924625499898326 38.35491086558666 24.01409882735715 34 13.756524096793873 23.337626245509387 41.062156849454354 21.84369280824239 35 13.681962990578187 22.803497593709753 38.2335796109266 25.280959804785468 36 14.799701755575137 25.014573307123978 38.54266928760252 21.64305564969837 37 14.018165796787093 25.419236765403646 39.204229648207146 21.358367789602113 38 13.809394699383176 26.48817189724124 36.08825323662984 23.614180166745747 39 16.483427099572967 24.019521453263742 37.914322510675795 21.582728936487495 40 16.813529451636956 23.23459635328408 38.488443028536565 21.4634311665424 41 15.37992272758083 24.840371449874603 37.77062292415102 22.009082898393547 42 16.040127431708807 25.356876567477798 37.95024740730699 20.652748593506406 43 17.56320748322375 25.249101877584216 36.240086762014506 20.947603877177524 44 16.836575611739985 25.8428794143564 35.19148647732664 22.129058496576967 45 16.934860706297027 24.05409069341829 34.848505388734495 24.162543211550194 46 18.171897241239073 24.872907205314174 34.34216769470616 22.613027858740594 47 15.482952619806142 24.65600216905036 37.693350504982035 22.16769470616146 48 16.05300616823697 24.66006913848031 35.58666034026977 23.700264353012944 49 16.200094895953367 23.766013692130414 37.783501660679185 22.250389751237037 50 15.564969836643394 22.799430624279807 37.60794414695316 24.027655392123638 51 15.10743577577442 21.911475632074833 36.303802616417 26.67728597573375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 268.0 1 326.0 2 384.0 3 4980.5 4 9577.0 5 6501.5 6 3426.0 7 2962.0 8 2498.0 9 2404.0 10 2310.0 11 2187.0 12 2064.0 13 1995.0 14 1926.0 15 1851.0 16 1776.0 17 1681.0 18 1586.0 19 1534.0 20 1482.0 21 1472.5 22 1463.0 23 1518.0 24 1573.0 25 1742.5 26 2034.0 27 2156.0 28 2433.0 29 2710.0 30 3083.0 31 3456.0 32 3764.5 33 4073.0 34 4351.5 35 4630.0 36 4957.5 37 5285.0 38 5681.0 39 6077.0 40 6427.0 41 6777.0 42 7455.5 43 8134.0 44 9670.5 45 11207.0 46 13086.5 47 14966.0 48 14206.0 49 13446.0 50 12002.5 51 10559.0 52 8667.5 53 6776.0 54 5489.5 55 4203.0 56 3619.5 57 3036.0 58 2737.0 59 2438.0 60 2177.0 61 1916.0 62 1660.5 63 1405.0 64 1114.5 65 824.0 66 640.5 67 457.0 68 364.0 69 271.0 70 236.5 71 202.0 72 173.0 73 144.0 74 105.0 75 46.5 76 27.0 77 23.0 78 19.0 79 14.0 80 9.0 81 8.0 82 7.0 83 6.0 84 5.0 85 3.5 86 2.0 87 2.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 147530.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.75498439085383 #Duplication Level Percentage of deduplicated Percentage of total 1 83.9239997600716 57.701932935217435 2 9.013999974230082 12.395148550546923 3 3.2509999907057905 6.705673608469278 4 1.5920002781641716 4.378318171016503 5 0.8610000006747519 2.9599020803458846 6 0.47599999867472725 1.9636423487356387 7 0.26499999924280326 1.275404956806065 8 0.15799999954830238 0.8690630002158753 9 0.10699999969410023 0.6621104977910303 >10 0.30799999911946585 3.4576881551309335 >50 0.025999999925669193 1.3606611372050343 >100 0.014999999957116842 1.9553917504856582 >500 9.99999997141123E-4 0.5142872817733025 >1k 0.001999999994282246 3.8007755262604497 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3600 2.4401816579678712 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1928 1.3068528434894597 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 748 0.5070155222666576 No Hit CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 391 0.2650308411848437 No Hit GAATCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTC 307 0.20809326916559345 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCG 303 0.20538195621229582 No Hit CCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC 229 0.15522266657628955 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 205 0.13895478885650375 No Hit GAATCTATCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTC 179 0.12133125466006914 No Hit GAATGAAACCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGCCG 178 0.12065342642174474 No Hit CCTGTCACTTATACACATCTGACGCTCACCTCTTCGTATGCCGTCTTCTGC 166 0.11251948756185182 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCTCTTCGTATGC 162 0.1098081746085542 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.18504710906256355 0.0 2 0.0 0.0 0.0 0.7693350504982037 0.0 3 6.778282383244086E-4 0.0 0.0 1.0614790212160239 0.0 4 6.778282383244086E-4 0.0 0.0 1.445129804107639 0.0 5 6.778282383244086E-4 0.0 0.0 2.6706432589981697 0.0 6 6.778282383244086E-4 0.0 0.0 3.6575611739985088 0.0 7 6.778282383244086E-4 0.0 0.0 4.692604893919881 0.0 8 6.778282383244086E-4 0.0 0.0 6.661695926252287 0.0 9 6.778282383244086E-4 0.0 0.0 7.54151697959737 0.0 10 6.778282383244086E-4 0.0 0.0 9.45095912695723 0.0 11 6.778282383244086E-4 0.0 0.0 11.025554124584831 0.0 12 6.778282383244086E-4 0.0 0.0 12.557445943197994 0.0 13 6.778282383244086E-4 0.0 0.0 13.202060597844506 0.0 14 6.778282383244086E-4 0.0 0.0 13.489459770894056 0.0 15 6.778282383244086E-4 0.0 0.0 13.932759438758218 0.0 16 6.778282383244086E-4 0.0 0.0 14.664136107910256 0.0 17 6.778282383244086E-4 0.0 0.0 15.515488375245713 0.0 18 6.778282383244086E-4 0.0 0.0 16.442757405273504 0.0 19 6.778282383244086E-4 0.0 0.0 16.98298651121806 0.0 20 6.778282383244086E-4 0.0 0.0 17.51237036534942 0.0 21 6.778282383244086E-4 0.0 0.0 18.163763302379177 0.0 22 6.778282383244086E-4 0.0 0.0 18.836846743035316 0.0 23 6.778282383244086E-4 0.0 0.0 19.522131091981294 0.0 24 6.778282383244086E-4 0.0 0.0 20.049481461397683 0.0 25 6.778282383244086E-4 0.0 0.0 20.490069816308548 0.0 26 6.778282383244086E-4 0.0 0.0 20.926591201789467 0.0 27 6.778282383244086E-4 0.0 0.0 21.352267335457196 0.0 28 6.778282383244086E-4 0.0 0.0 21.807767911611197 0.0 29 6.778282383244086E-4 0.0 0.0 22.25716803362028 0.0 30 6.778282383244086E-4 0.0 0.0 22.777740120653426 0.0 31 6.778282383244086E-4 0.0 0.0 23.227818070900835 0.0 32 6.778282383244086E-4 0.0 0.0 23.66095031519013 0.0 33 6.778282383244086E-4 0.0 0.0 24.121195689012403 0.0 34 6.778282383244086E-4 0.0 0.0 24.56313970039992 0.0 35 6.778282383244086E-4 0.0 0.0 25.001016742357486 0.0 36 6.778282383244086E-4 0.0 0.0 25.442960753745 0.0 37 0.0013556564766488172 0.0 0.0 25.875415169795975 0.0 38 0.002033484714973226 0.0 0.0 26.281434284552294 0.0 39 0.002033484714973226 0.0 0.0 26.70982173117332 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTATT 30 2.1526084E-6 45.000004 3 TACGGCG 30 2.1526084E-6 45.000004 15 CATTCAA 30 2.1526084E-6 45.000004 1 TGCGAAG 30 2.1526084E-6 45.000004 2 ATGGGTC 20 7.0132833E-4 45.0 5 GAGTGTC 20 7.0132833E-4 45.0 11 CGGGTAA 20 7.0132833E-4 45.0 6 TAGAGGC 20 7.0132833E-4 45.0 4 ATCTATC 70 0.0 45.0 3 GGATGAC 25 3.874235E-5 45.0 8 GGCACCG 20 7.0132833E-4 45.0 8 GGATCAC 20 7.0132833E-4 45.0 8 TAACGGG 35 1.2023156E-7 45.0 3 TGAAACG 20 7.0132833E-4 45.0 4 ACGGGAC 25 3.874235E-5 45.0 5 GTTAATC 20 7.0132833E-4 45.0 40 CTACGGG 20 7.0132833E-4 45.0 3 TTGAACG 20 7.0132833E-4 45.0 14 CGTAAGG 35 1.2023156E-7 45.0 3 TTACGGG 35 1.2023156E-7 45.0 3 >>END_MODULE