##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936180.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79886
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57379265453271	31.0	31.0	34.0	30.0	34.0
2	31.732719124752773	31.0	31.0	34.0	30.0	34.0
3	30.530355756953657	31.0	30.0	34.0	25.0	34.0
4	34.90954610319706	37.0	35.0	37.0	32.0	37.0
5	31.44321908719926	35.0	35.0	37.0	0.0	37.0
6	33.59722604711714	36.0	35.0	37.0	17.0	37.0
7	21.574030493453172	32.0	0.0	37.0	0.0	37.0
8	28.549207620859725	35.0	17.0	37.0	17.0	37.0
9	35.055566682522596	37.0	32.0	39.0	32.0	39.0
10	36.533172270485444	37.0	35.0	39.0	33.0	39.0
11	36.86878802293268	39.0	37.0	39.0	33.0	39.0
12	37.31802819017099	39.0	37.0	39.0	34.0	39.0
13	37.245011641589265	39.0	37.0	39.0	34.0	39.0
14	38.763525523871515	40.0	38.0	41.0	35.0	41.0
15	38.866422151565985	40.0	38.0	41.0	35.0	41.0
16	38.845579951430786	40.0	38.0	41.0	35.0	41.0
17	38.799889843026314	40.0	38.0	41.0	35.0	41.0
18	38.5290038304584	40.0	38.0	41.0	35.0	41.0
19	38.25652805247478	40.0	37.0	41.0	34.0	41.0
20	37.99594422051423	40.0	36.0	41.0	34.0	41.0
21	37.87905265002629	39.0	35.0	41.0	33.0	41.0
22	37.696116966677515	39.0	35.0	41.0	33.0	41.0
23	37.529254187216786	39.0	35.0	41.0	33.0	41.0
24	37.29318028190171	39.0	35.0	41.0	33.0	41.0
25	37.13032321057507	39.0	35.0	41.0	33.0	41.0
26	37.01632326064642	39.0	35.0	40.0	33.0	41.0
27	36.956851012693086	39.0	35.0	40.0	33.0	41.0
28	36.91535437999149	39.0	35.0	40.0	33.0	41.0
29	36.9136519540345	39.0	35.0	40.0	33.0	41.0
30	36.754037002728886	38.0	35.0	40.0	33.0	41.0
31	36.5567558771249	38.0	35.0	40.0	32.0	41.0
32	36.24606313997447	38.0	35.0	40.0	31.0	41.0
33	35.67300903787898	38.0	34.0	40.0	26.0	41.0
34	34.874032997020755	38.0	33.0	40.0	21.0	41.0
35	34.08165385674586	38.0	33.0	40.0	16.0	41.0
36	33.47468893172771	38.0	33.0	40.0	14.0	41.0
37	33.08657336704805	38.0	33.0	40.0	10.0	41.0
38	32.82220914803595	38.0	32.0	40.0	10.0	41.0
39	32.65593470695742	38.0	31.0	40.0	9.0	41.0
40	32.524334676914606	38.0	31.0	40.0	8.0	41.0
41	32.33337505945973	38.0	30.0	40.0	8.0	41.0
42	32.13127456625691	38.0	30.0	40.0	8.0	41.0
43	31.982149563127457	38.0	30.0	40.0	8.0	41.0
44	31.78992564404276	38.0	29.0	40.0	8.0	41.0
45	31.737363242620734	38.0	29.0	40.0	8.0	41.0
46	31.559171819843275	37.0	27.0	40.0	7.0	41.0
47	31.47123400846206	37.0	27.0	40.0	7.0	41.0
48	31.306962421450567	37.0	26.0	40.0	7.0	41.0
49	31.14484390256115	37.0	26.0	40.0	7.0	41.0
50	31.002941691910973	37.0	25.0	40.0	7.0	41.0
51	29.50128933730566	35.0	22.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	2.0
12	0.0
13	2.0
14	1.0
15	3.0
16	6.0
17	13.0
18	29.0
19	69.0
20	139.0
21	237.0
22	486.0
23	866.0
24	1561.0
25	2534.0
26	3265.0
27	3183.0
28	2695.0
29	2385.0
30	2104.0
31	2134.0
32	2531.0
33	3264.0
34	4322.0
35	5632.0
36	7159.0
37	12490.0
38	13370.0
39	9398.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.384723230603612	17.123150489447465	21.057506947400046	30.434619332548884
2	45.53113186290464	21.573241869664272	20.301429537090353	12.594196730340734
3	25.84307638384698	21.231504894474625	40.882006859775174	12.043411861903213
4	20.937335703377315	21.46684024735248	43.599629471997595	13.996194577272615
5	18.453796660240844	32.254712965976516	36.61717948076008	12.67431089302256
6	19.835765966502265	28.89492526850762	40.764339183336254	10.504969581653857
7	38.54743008787522	40.12217409808978	19.23991688155622	2.0904789324787822
8	66.80519740630399	4.506421650852465	26.222366872793728	2.466014070049821
9	61.796810454898235	6.906091179931402	27.348972285506846	3.9481260796635205
10	28.043712290013268	30.218060736549585	32.861828105049696	8.87639886838745
11	18.468818065743683	22.388153118193426	45.58495856595649	13.558070250106402
12	17.14693438149363	21.13887289387377	46.85051197957088	14.863680745061714
13	16.805197406303986	21.291590516485993	48.42525598978544	13.47795608742458
14	15.539643992689584	24.185714643366797	45.5173622411937	14.75727912274992
15	15.294294369476505	24.015472047667927	47.35372906391608	13.336504518939488
16	18.30107903762862	23.98417745287034	44.30563553063115	13.409107978869889
17	18.15712390155972	23.331998097288636	44.810104398768246	13.700773602383395
18	17.69396389855544	23.41336404376236	45.16185564429312	13.73081641338908
19	16.859024109355833	24.645119294995368	44.13163758355657	14.36421901209223
20	17.727762060936836	24.600055078486843	44.46586385599479	13.206319004581529
21	17.38602508574719	24.09934156172546	45.3371053751596	13.177527977367747
22	17.586310492451744	21.74598803294695	45.46228375434995	15.205417720251358
23	15.696116966677515	24.277094860175747	45.6625691610545	14.36421901209223
24	16.14550734797086	23.379565881380966	45.170618130836445	15.304308639811731
25	15.637283128458051	25.40370027288887	44.26057131412263	14.698445284530456
26	16.266930375785492	25.22845054202238	43.76110958115314	14.743509501038982
27	16.191823348271285	25.321082542623234	44.12537866459705	14.361715444508425
28	14.912500312945948	24.66014070049821	45.93170267631375	14.495656310242094
29	15.981523671231503	23.885286533309966	44.93277921037478	15.200410585083745
30	16.056630698745714	24.65262999774679	44.977843426883304	14.312895876624191
31	17.327191247527725	23.964148912199885	43.88628796034349	14.8223718799289
32	16.22562151065268	24.739003079388127	43.822446986956415	15.212928423002777
33	15.991537941566733	25.557669679292992	43.078887414565756	15.371904964574517
34	16.931627569286235	24.99687054052024	43.33550309190596	14.735998798287559
35	16.15426983451418	26.93713541797061	40.9608692386651	15.94772550885011
36	17.7878476829482	28.05247477655659	39.258443281676385	14.901234258818816
37	17.54500162731893	28.551936509526076	38.39846781663871	15.504594046516285
38	17.93555817039281	28.91870916055379	36.62719375109531	16.51853891795809
39	18.655333850737303	28.08376937135418	36.781163157499435	16.479733620409082
40	18.93322985253987	27.92604461357434	37.03527526725584	16.105450266629948
41	18.702901634829633	27.941066019077187	36.1552712615477	17.200761084545476
42	18.60025536389355	27.953583856996218	36.72608467065569	16.72007610845455
43	19.495280775104522	27.750794882707858	35.77598077260096	16.977943569586664
44	18.388703903061863	28.059985479308015	35.47680444633603	18.074506171294093
45	18.393711038229476	27.651903963147483	35.3240868237238	18.63029817489923
46	18.511378714668403	28.630798888415992	34.802092982500064	18.05572941441554
47	17.608842600706005	28.429261697919532	36.43692261472599	17.524973086648473
48	18.455048444032744	28.39921888691385	35.01489622712365	18.13083644192975
49	18.56395363392835	27.539243421876176	36.285456775905665	17.611346168289813
50	17.92053676488997	27.106126229877574	35.75344866434669	19.219888340885763
51	17.50118919460231	26.870790876999724	35.4655383922089	20.16248153618907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	126.0
2	166.0
3	2994.0
4	5822.0
5	4125.0
6	2428.0
7	2092.0
8	1756.0
9	1648.0
10	1540.0
11	1496.5
12	1453.0
13	1379.5
14	1306.0
15	1240.0
16	1174.0
17	1117.5
18	1061.0
19	975.0
20	889.0
21	887.5
22	886.0
23	943.5
24	1001.0
25	1080.5
26	1326.0
27	1492.0
28	1672.0
29	1852.0
30	2092.0
31	2332.0
32	2608.0
33	2884.0
34	3164.5
35	3445.0
36	3632.0
37	3819.0
38	3927.0
39	4035.0
40	4141.5
41	4248.0
42	4471.5
43	4695.0
44	4876.0
45	5057.0
46	5318.0
47	5579.0
48	5197.5
49	4816.0
50	4512.5
51	4209.0
52	3647.5
53	3086.0
54	2716.5
55	2347.0
56	2030.0
57	1713.0
58	1503.0
59	1293.0
60	1110.0
61	927.0
62	756.5
63	586.0
64	474.5
65	363.0
66	270.5
67	178.0
68	141.5
69	105.0
70	72.5
71	40.0
72	29.0
73	18.0
74	15.0
75	11.5
76	11.0
77	10.5
78	10.0
79	6.5
80	3.0
81	2.0
82	1.0
83	1.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	79886.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.35059960443631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.2848887504758	60.25711639085697
2	10.187203709470916	14.741005933455172
3	3.4949306204366932	7.585809778934982
4	1.5260043600124573	4.416293217835415
5	0.6401605591888992	2.3158000150214053
6	0.3443025710232188	1.4946298475327342
7	0.16955604000138413	0.8587236812457752
8	0.09342883836810963	0.5407705981022958
9	0.057095401224955884	0.37177978619532837
>10	0.18339734938925223	2.1881180682472525
>50	0.01038098204090107	0.5833312470270134
>100	0.005190491020450535	0.9075432491300103
>500	0.0	0.0
>1k	0.003460327346967023	3.7390781864156426
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1939	2.427208772500814	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1048	1.3118694139148286	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	397	0.49695816538567456	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	226	0.28290313697018254	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGC	102	0.12768194677415318	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97	0.12142302781463585	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTC	88	0.1101569736875047	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05758205442755927	0.0
2	0.0	0.0	0.0	0.25160854217259593	0.0
3	0.0	0.0	0.0	0.3442405427734522	0.0
4	0.0	0.0	0.0	0.4881956788423503	0.0
5	0.0	0.0	0.0	0.9062914653381068	0.0
6	0.0	0.0	0.0	1.1816838995568686	0.0
7	0.0	0.0	0.0	1.483363793405603	0.0
8	0.0	0.0	0.0	2.049170067345968	0.0
9	0.0	0.0	0.0	2.344591042235185	0.0
10	0.0	0.0	0.0	3.043086398117317	0.0
11	0.0	0.0	0.0	3.5963748341386474	0.0
12	0.0	0.0	0.0	4.2973737576045865	0.0
13	0.0	0.0	0.0	4.49765916430914	0.0
14	0.0	0.0	0.0	4.61657862453997	0.0
15	0.0	0.0	0.0	4.7880730040307435	0.0
16	0.0	0.0	0.0	4.977092356608167	0.0
17	0.0	0.0	0.0	5.331347169716847	0.0
18	0.0	0.0	0.0	5.670580577322685	0.0
19	0.0	0.0	0.0	5.9059159302005355	0.0
20	0.0	0.0	0.0	6.1237263099917385	0.0
21	0.0	0.0	0.0	6.384097338707658	0.0
22	0.0	0.0	0.0	6.625691610545027	0.0
23	0.0	0.0	0.0	6.898580477179982	0.0
24	0.0	0.0	0.0	7.112635505595474	0.0
25	0.0	0.0	0.0	7.341711939513807	0.0
26	0.0	0.0	0.0	7.548256265177878	0.0
27	0.0	0.0	0.0	7.751045239466239	0.0
28	0.0	0.0	0.0	7.987632376135994	0.0
29	0.0	0.0	0.0	8.259269458979045	0.0
30	0.0	0.0	0.0	8.51087800115164	0.0
31	0.0	0.0	0.0	8.756227624364719	0.0
32	0.0	0.0	0.0	8.99531832861828	0.0
33	0.0	0.0	0.0	9.249430438374684	0.0
34	0.0	0.0	0.0	9.50479433192299	0.0
35	0.0	0.0	0.0	9.742633252384648	0.0
36	0.0	0.0	0.0	9.990486443181533	0.0
37	0.0	0.0	0.0	10.222066444683675	0.0
38	0.0	0.0	0.0	10.499962446486244	0.0
39	0.0	0.0	0.0	10.82792979996495	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAGGC	30	2.1401356E-6	45.000004	4
CAAGGAT	30	2.1401356E-6	45.000004	5
ATCTATC	25	3.8581777E-5	45.0	3
AGGAGGC	35	1.1930388E-7	45.0	7
CTATCTC	45	3.7471182E-10	45.0	5
GTAGGGC	20	6.993879E-4	45.0	5
ACGGGAA	20	6.993879E-4	45.0	5
CGTTATT	235	0.0	45.0	1
CAGGGTC	20	6.993879E-4	45.0	6
ATAAGGA	25	3.8581777E-5	45.0	4
GCTAGGA	20	6.993879E-4	45.0	4
TAGGCGA	20	6.993879E-4	45.0	6
GGCGATA	20	6.993879E-4	45.0	8
CGAAGGA	25	3.8581777E-5	45.0	4
CGTTTTA	425	0.0	44.470585	1
CGTTTTT	975	0.0	43.615387	1
CGGTTTT	110	0.0	40.909092	1
CGGTTTA	55	5.820766E-11	40.909092	1
GTTTTAT	465	0.0	40.64516	2
AGGCGAT	45	1.88993E-8	40.0	7
>>END_MODULE