##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936179.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	81070
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.291316146540026	31.0	31.0	34.0	28.0	34.0
2	31.468015295423708	31.0	31.0	34.0	30.0	34.0
3	30.74093992845689	31.0	30.0	34.0	26.0	34.0
4	34.92703836190946	35.0	35.0	37.0	32.0	37.0
5	31.56903910201061	35.0	35.0	37.0	0.0	37.0
6	33.534538053533986	35.0	35.0	37.0	17.0	37.0
7	21.252991242136424	32.0	0.0	37.0	0.0	37.0
8	28.273590724065624	35.0	17.0	37.0	17.0	37.0
9	34.52428765264586	35.0	32.0	39.0	28.0	39.0
10	35.87303564820525	37.0	35.0	39.0	32.0	39.0
11	36.36142839521401	37.0	35.0	39.0	32.0	39.0
12	36.844023683236706	39.0	35.0	39.0	33.0	39.0
13	36.70045639570741	39.0	35.0	39.0	33.0	39.0
14	38.03807820402122	40.0	37.0	41.0	33.0	41.0
15	38.1531022573085	40.0	37.0	41.0	33.0	41.0
16	38.17381275440977	40.0	37.0	41.0	33.0	41.0
17	38.12875292956704	40.0	37.0	41.0	33.0	41.0
18	37.92727272727273	39.0	37.0	40.0	33.0	41.0
19	37.760848649315406	39.0	37.0	40.0	33.0	41.0
20	37.58586406808931	39.0	36.0	40.0	33.0	41.0
21	37.446897742691505	39.0	35.0	40.0	33.0	41.0
22	37.405735783890464	39.0	35.0	40.0	33.0	41.0
23	37.26728752929567	39.0	35.0	40.0	33.0	41.0
24	37.065363266313064	39.0	35.0	40.0	32.0	41.0
25	36.898088072036515	38.0	35.0	40.0	32.0	41.0
26	36.75717281361786	38.0	35.0	40.0	32.0	41.0
27	36.707252991242136	38.0	35.0	40.0	32.0	41.0
28	36.650770938694954	38.0	35.0	40.0	32.0	41.0
29	36.640606882940666	38.0	35.0	40.0	31.0	41.0
30	36.41779943258912	38.0	35.0	40.0	31.0	41.0
31	36.22568150980634	38.0	35.0	40.0	30.0	41.0
32	36.00909090909091	38.0	35.0	40.0	30.0	41.0
33	35.64646601702233	38.0	34.0	40.0	27.0	41.0
34	35.185691377821634	38.0	34.0	40.0	25.0	41.0
35	34.73173800419391	38.0	33.0	40.0	23.0	41.0
36	34.312248673985444	38.0	33.0	40.0	21.0	41.0
37	34.157974589860615	38.0	33.0	40.0	18.0	41.0
38	33.911459232761814	38.0	33.0	40.0	18.0	41.0
39	33.7616134205008	38.0	33.0	40.0	16.0	41.0
40	33.564758850376215	38.0	33.0	40.0	15.0	41.0
41	33.38882447267793	38.0	33.0	40.0	15.0	41.0
42	33.32332552115456	38.0	33.0	40.0	15.0	41.0
43	33.176551128654246	37.0	32.0	40.0	14.0	41.0
44	32.9391513506846	37.0	32.0	40.0	12.0	41.0
45	32.7483656099667	37.0	31.0	40.0	12.0	41.0
46	32.50254101393857	37.0	31.0	40.0	10.0	41.0
47	32.46522758110275	37.0	31.0	40.0	10.0	41.0
48	32.293265079560875	37.0	31.0	40.0	10.0	41.0
49	32.08567904280252	36.0	30.0	40.0	10.0	41.0
50	31.794942642161097	36.0	29.0	40.0	10.0	41.0
51	29.717700752436166	34.0	24.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	2.0
12	3.0
13	2.0
14	0.0
15	4.0
16	7.0
17	12.0
18	35.0
19	68.0
20	139.0
21	240.0
22	462.0
23	720.0
24	1164.0
25	1770.0
26	2156.0
27	2363.0
28	2267.0
29	2375.0
30	2456.0
31	2836.0
32	3578.0
33	4698.0
34	5976.0
35	7739.0
36	9130.0
37	12761.0
38	11362.0
39	6741.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.9981497471321	18.467990625385468	23.353891698532134	23.17996792895029
2	39.11557912914765	23.347724188972492	21.79844578759097	15.738250894288885
3	26.300727766128034	23.559886517824104	36.01948933020846	14.119896385839398
4	22.96903910201061	23.381028740594548	37.05563093622795	16.594301221166894
5	18.319970395954112	33.68940421857654	31.921795978783766	16.06882940668558
6	20.85358332305415	30.772172196866904	35.790057974589864	12.584186505489084
7	40.270136918712225	41.56408042432466	14.853830023436537	3.3119526335265816
8	66.69051437029727	6.607869742198099	21.443197236955715	5.258418650548909
9	61.11261872455902	9.024299987664982	23.57222153694338	6.290859750832614
10	27.912914765017888	29.964228444554088	30.717898112742077	11.40495867768595
11	20.31947699518934	24.813124460342912	38.199087208585176	16.66831133588257
12	19.232761810780808	22.574318490193658	41.06697915381769	17.125940545207847
13	18.00049340076477	24.72554582459603	42.37572468237325	14.898236092265943
14	16.065128900949794	26.602935734550385	39.802639694091525	17.529295670408292
15	14.345627235722217	26.25138768965092	43.25521154557789	16.14777352904897
16	16.515357098803506	26.515357098803506	39.617614407302334	17.351671395090662
17	16.910077710620453	25.522388059701495	38.50129517700752	19.066239052670532
18	17.185148636980387	24.784753916368572	41.16935981250771	16.860737634143334
19	16.362402861724433	26.25015418773899	38.94658936721352	18.440853583323054
20	18.13001110151721	26.243986678179347	39.54853830023436	16.077463920069075
21	16.435179474528187	27.55396570864685	39.89638583939805	16.114468977426917
22	16.330331812014308	25.162205501418526	37.85123966942149	20.656223017145678
23	14.78352041445664	26.83976810164056	38.97989391883557	19.396817565067227
24	16.62513876896509	24.316023189835946	39.52140125817195	19.53743678302701
25	15.265819662020478	27.866041692364625	37.3356358702356	19.5325027753793
26	16.16627605772789	26.380905390403353	38.17565067225854	19.277167879610214
27	16.805230048106576	26.393240409522633	37.1592450968299	19.64228444554089
28	13.40446527692118	25.933144196373505	39.00209695325027	21.66029357345504
29	17.239422721105218	23.833723942272112	37.46268656716418	21.464166769458494
30	16.38460589613914	24.987048229924756	37.79819908720859	20.830146786727518
31	16.379671888491426	26.47465153570988	35.818428518564204	21.32724805723449
32	16.865671641791042	25.2140125817195	36.86443813987912	21.055877636610337
33	15.140002467003825	24.383865794991983	38.227457752559516	22.24867398544468
34	15.149870482299246	24.843962008141112	38.538300234365366	21.467867275194276
35	15.574195140002466	24.423337856173678	37.986924879733564	22.01554212409029
36	16.515357098803506	26.36486986554829	37.0223263846059	20.097446651042308
37	14.980880720365116	26.36857037128408	36.50918958924386	22.141359319106947
38	14.292586653509312	27.19131614654003	36.28222523744912	22.233871962501542
39	16.611570247933884	25.34229678056001	37.08030097446651	20.965831997039597
40	18.38164549155051	25.369433822622423	37.08153447637844	19.167386209448626
41	16.461083014678675	26.034291353151595	35.283088688787466	22.22153694338226
42	17.979523868261996	24.20254101393857	36.482052547181446	21.335882570617983
43	18.46182311582583	24.145799925989884	34.859997532996175	22.53237942518811
44	17.269026766991487	24.448007894412235	33.694338226224254	24.588627112372023
45	18.123843591957566	24.280251634390034	33.08498828173184	24.51091649192056
46	18.604909337609474	25.126433945972614	34.33945972616257	21.929196990255335
47	15.43357592204268	24.829159985197975	37.41457999259899	22.322684100160355
48	16.216849636116937	23.855926976686813	36.03552485506353	23.891698532132725
49	17.99555939311706	22.3732576785494	37.23942272110522	22.39176020722832
50	16.494387566300727	23.171333415566796	36.331565313926234	24.00271370420624
51	15.539657086468484	22.61625755519921	34.77241889724929	27.071666461083016
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	227.0
1	277.0
2	327.0
3	2364.5
4	4402.0
5	2947.5
6	1493.0
7	1312.0
8	1131.0
9	1061.5
10	992.0
11	969.5
12	947.0
13	913.5
14	880.0
15	856.0
16	832.0
17	770.0
18	708.0
19	725.5
20	743.0
21	769.0
22	795.0
23	780.0
24	765.0
25	855.0
26	1025.0
27	1105.0
28	1283.5
29	1462.0
30	1622.0
31	1782.0
32	2032.5
33	2283.0
34	2431.0
35	2579.0
36	2757.0
37	2935.0
38	3141.5
39	3348.0
40	3671.0
41	3994.0
42	4468.5
43	4943.0
44	5540.0
45	6137.0
46	7055.0
47	7973.0
48	8020.5
49	8068.0
50	7350.5
51	6633.0
52	5465.0
53	4297.0
54	3552.5
55	2808.0
56	2357.5
57	1907.0
58	1619.5
59	1332.0
60	1120.5
61	909.0
62	764.0
63	619.0
64	484.5
65	350.0
66	274.5
67	199.0
68	159.0
69	119.0
70	77.5
71	36.0
72	30.0
73	24.0
74	21.5
75	13.0
76	7.0
77	8.0
78	9.0
79	6.5
80	4.0
81	2.5
82	1.0
83	1.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	81070.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.7758727026027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.74986928788037	59.27470087578636
2	9.174247969605075	12.9863081287776
3	3.3741155146571855	7.164179104477612
4	1.6434870507860153	4.652769211792278
5	0.8139007982153439	2.880226964351795
6	0.4269929241172575	1.8132478105341063
7	0.2561957544703545	1.2692734673738744
8	0.16382585660009064	0.9275934377698285
9	0.07494161525323295	0.47736523991612184
>10	0.29105231970441636	3.162698902183298
>50	0.01917111087873401	1.0509436289626248
>100	0.00871414130851546	1.1755273220673492
>500	0.0017428282617030916	1.1286542494140865
>1k	0.0017428282617030916	2.0365116565930674
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1651	2.0365116565930674	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	915	1.1286542494140865	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	375	0.4625632169729863	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	175	0.21586283458739358	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	165	0.20352781546811397	No Hit
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	137	0.16898976193413098	RNA PCR Primer, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	101	0.12458369310472432	Illumina PCR Primer Index 6 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	98	0.12088318736894044	RNA PCR Primer, Index 27 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	94	0.11594917972122856	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	92	0.11348217589737264	RNA PCR Primer, Index 27 (96% over 25bp)
GCTGTCACTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	85	0.1048476625138769	Illumina PCR Primer Index 6 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	83	0.10238065869002098	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19119279634883435	0.0
2	0.0	0.0	0.0	0.8609843345257185	0.0
3	0.0	0.0	0.0	1.1286542494140865	0.0
4	0.0	0.0	0.0	1.4431972369557173	0.0
5	0.0	0.0	0.0	2.4818058467990625	0.0
6	0.0	0.0	0.0	3.150363883064019	0.0
7	0.0	0.0	0.0	3.917602072283212	0.0
8	0.0	0.0	0.0	5.603799185888738	0.0
9	0.0	0.0	0.0	6.205748118909584	0.0
10	0.0	0.0	0.0	7.809300604415937	0.0
11	0.0	0.0	0.0	9.69039102010608	0.0
12	0.0	0.0	0.0	11.359319106944616	0.0
13	0.0	0.0	0.0	12.035278154681139	0.0
14	0.0	0.0	0.0	12.306648575305292	0.0
15	0.0	0.0	0.0	12.832120389786605	0.0
16	0.0	0.0	0.0	13.913901566547429	0.0
17	0.0	0.0	0.0	15.15480448994696	0.0
18	0.0	0.0	0.0	16.33526581966202	0.0
19	0.0	0.0	0.0	17.066732453435304	0.0
20	0.0	0.0	0.0	17.7944985814728	0.0
21	0.0	0.0	0.0	18.7208585173307	0.0
22	0.0	0.0	0.0	19.63611693598125	0.0
23	0.0	0.0	0.0	20.54520784507216	0.0
24	0.0	0.0	0.0	21.287775996052794	0.0
25	0.0	0.0	0.0	21.947699518934254	0.0
26	0.0	0.0	0.0	22.552115455778956	0.0
27	0.0	0.0	0.0	23.205871469100778	0.0
28	0.0	0.0	0.0	23.8509929690391	0.0
29	0.0	0.0	0.0	24.467743925003084	0.0
30	0.0	0.0	0.0	25.21031207598372	0.0
31	0.0	0.0	0.0	25.897372640927593	0.0
32	0.0	0.0	0.0	26.527692117922783	0.0
33	0.0	0.0	0.0	27.143209571974836	0.0
34	0.0	0.0	0.0	27.70445294190206	0.0
35	0.0	0.0	0.0	28.416183545084493	0.0
36	0.0	0.0	0.0	29.062538546934746	0.0
37	0.0	0.0	0.0	29.674355495251017	0.0
38	0.0	0.0	0.0	30.239299370914026	0.0
39	0.0	0.0	0.0	30.88442087085235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTTT	45	3.7471182E-10	45.000004	1
AGGGGCA	20	6.994505E-4	45.0	5
CGTTAGG	20	6.994505E-4	45.0	3
ATGATGG	20	6.994505E-4	45.0	2
TATAAGG	25	3.8586964E-5	45.0	3
TGTCACT	70	0.0	45.0	3
GGGGGTC	20	6.994505E-4	45.0	6
AGACGGG	20	6.994505E-4	45.0	3
AAAGGTA	20	6.994505E-4	45.0	5
ACGGGCG	20	6.994505E-4	45.0	5
TTAGGCT	20	6.994505E-4	45.0	5
AGCATAG	20	6.994505E-4	45.0	2
AAGGATA	20	6.994505E-4	45.0	6
TCACTTA	70	0.0	45.0	5
CGTTGTT	20	6.994505E-4	45.0	1
CGGTTTA	25	3.8586964E-5	45.0	1
CGTTTTA	335	0.0	45.0	1
AGGTATG	30	2.1405376E-6	44.999996	7
CGTTTTT	620	0.0	43.54839	1
CGTTATT	110	0.0	42.954544	1
>>END_MODULE