##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936178.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102564
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.586931086931088	31.0	31.0	34.0	30.0	34.0
2	31.744832494832494	31.0	31.0	34.0	30.0	34.0
3	30.67027417027417	31.0	30.0	34.0	26.0	34.0
4	35.00880425880426	37.0	35.0	37.0	32.0	37.0
5	31.55881205881206	35.0	35.0	37.0	0.0	37.0
6	33.667124917124916	37.0	35.0	37.0	17.0	37.0
7	21.47263172263172	32.0	0.0	37.0	0.0	37.0
8	28.448149448149447	35.0	17.0	37.0	17.0	37.0
9	34.876379626379624	37.0	32.0	39.0	31.0	39.0
10	36.37496587496587	37.0	35.0	39.0	32.0	39.0
11	36.87013962013962	39.0	37.0	39.0	33.0	39.0
12	37.248030498030495	39.0	37.0	39.0	34.0	39.0
13	37.13193713193713	39.0	37.0	39.0	34.0	39.0
14	38.63193713193713	40.0	38.0	41.0	35.0	41.0
15	38.73647673647674	40.0	38.0	41.0	35.0	41.0
16	38.738923988923986	40.0	38.0	41.0	35.0	41.0
17	38.67596817596817	40.0	38.0	41.0	35.0	41.0
18	38.43809718809719	40.0	38.0	41.0	34.0	41.0
19	38.217737217737216	40.0	37.0	41.0	34.0	41.0
20	37.96980421980422	40.0	36.0	41.0	34.0	41.0
21	37.845657345657344	40.0	35.0	41.0	33.0	41.0
22	37.66970866970867	39.0	35.0	41.0	33.0	41.0
23	37.53173628173628	39.0	35.0	41.0	33.0	41.0
24	37.26696501696502	39.0	35.0	41.0	33.0	41.0
25	37.11432861432861	39.0	35.0	40.0	33.0	41.0
26	36.961594711594714	39.0	35.0	40.0	33.0	41.0
27	36.901992901992905	39.0	35.0	40.0	33.0	41.0
28	36.81054756054756	38.0	35.0	40.0	33.0	41.0
29	36.781531531531535	38.0	35.0	40.0	33.0	41.0
30	36.61024336024336	38.0	35.0	40.0	32.0	41.0
31	36.392964392964394	38.0	35.0	40.0	31.0	41.0
32	36.12540462540463	38.0	35.0	40.0	30.0	41.0
33	35.64903864903865	38.0	34.0	40.0	27.0	41.0
34	35.02039702039702	38.0	34.0	40.0	23.0	41.0
35	34.453765453765456	38.0	33.0	40.0	20.0	41.0
36	33.95538395538396	38.0	33.0	40.0	16.0	41.0
37	33.68967668967669	38.0	33.0	40.0	15.0	41.0
38	33.49807924807925	38.0	33.0	40.0	12.0	41.0
39	33.36018486018486	38.0	33.0	40.0	10.0	41.0
40	33.266731016731015	38.0	33.0	40.0	10.0	41.0
41	33.0994110994111	38.0	33.0	40.0	10.0	41.0
42	32.964626964626966	38.0	33.0	40.0	10.0	41.0
43	32.8515561015561	38.0	32.0	40.0	10.0	41.0
44	32.70952770952771	38.0	32.0	40.0	10.0	41.0
45	32.589524589524586	37.0	31.0	40.0	10.0	41.0
46	32.44270894270894	37.0	31.0	40.0	10.0	41.0
47	32.40755040755041	37.0	31.0	40.0	10.0	41.0
48	32.32689832689832	37.0	31.0	40.0	10.0	41.0
49	32.23311298311298	37.0	31.0	40.0	10.0	41.0
50	32.0969150969151	37.0	30.0	40.0	10.0	41.0
51	30.57037557037557	35.0	26.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	1.0
11	4.0
12	2.0
13	2.0
14	2.0
15	7.0
16	7.0
17	23.0
18	47.0
19	90.0
20	179.0
21	283.0
22	517.0
23	952.0
24	1675.0
25	2548.0
26	3269.0
27	3491.0
28	3215.0
29	2651.0
30	2604.0
31	2956.0
32	3546.0
33	5094.0
34	6551.0
35	8627.0
36	9863.0
37	16051.0
38	16746.0
39	11556.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.494832494832494	17.369642369642367	21.01127101127101	29.124254124254122
2	43.48991848991849	22.42989742989743	20.664170664170666	13.416013416013417
3	27.312702312702314	20.915720915720915	39.80928980928981	11.962286962286962
4	23.002223002223	21.330096330096328	40.9012909012909	14.766389766389768
5	19.24944424944425	32.898482898482904	34.443859443859445	13.408213408213406
6	19.690144690144688	30.08268008268008	38.96786396786397	11.259311259311259
7	38.097188097188095	41.06996606996607	18.312468312468315	2.5203775203775205
8	63.82161382161382	6.888381888381888	26.05105105105105	3.238953238953239
9	58.45325845325845	8.487383487383488	27.796302796302797	5.2630552630552625
10	29.30950430950431	24.405249405249403	34.20595920595921	12.079287079287079
11	22.39089739089739	23.434148434148433	39.96236496236496	14.212589212589213
12	20.785070785070786	20.932295932295933	43.55816855816856	14.724464724464726
13	17.77036777036777	21.723021723021724	44.51074451074451	15.995865995865996
14	15.206115206115205	23.76077376077376	44.20849420849421	16.824616824616825
15	15.248040248040247	23.363948363948364	46.431496431496434	14.956514956514958
16	17.466167466167466	23.668148668148667	43.373893373893374	15.491790491790491
17	17.28969228969229	23.04219804219804	42.780117780117784	16.887991887991888
18	16.575991575991576	23.8007488007488	43.67321867321867	15.95004095004095
19	17.593892593892594	24.255099255099253	42.387192387192385	15.763815763815764
20	18.77851877851878	23.61062361062361	43.25689325689326	14.353964353964354
21	18.545493545493546	23.825123825123825	43.051168051168055	14.57821457821458
22	17.66994266994267	22.315822315822313	42.97706797706798	17.037167037167038
23	16.93089193089193	23.749073749073748	43.39241839241839	15.927615927615927
24	16.803166803166803	22.883272883272884	43.79021879021879	16.52334152334152
25	16.72906672906673	24.686049686049685	41.95721695721696	16.627666627666628
26	15.83109083109083	24.64217464217464	42.994617994618	16.532116532116532
27	16.784641784641785	24.064974064974066	43.24811824811825	15.902265902265903
28	14.842439842439841	25.429000429000432	43.40606840606841	16.322491322491324
29	16.328341328341327	23.638898638898638	43.678093678093674	16.354666354666357
30	16.887016887016888	24.391599391599392	42.704067704067704	16.017316017316016
31	17.362817362817363	24.54954954954955	41.891891891891895	16.195741195741196
32	17.20974220974221	25.473850473850472	41.47556647556648	15.840840840840842
33	17.54026754026754	24.66947466947467	40.79209079209079	16.998166998166997
34	16.53991653991654	25.06922506922507	41.467766467766474	16.923091923091924
35	17.48079248079248	25.095550095550095	39.3032643032643	18.12039312039312
36	17.723567723567722	28.84150384150384	37.196287196287194	16.23864123864124
37	17.547092547092547	28.738153738153738	37.13193713193713	16.582816582816584
38	17.203892203892206	29.35825435825436	35.88588588588589	17.55196755196755
39	18.08919308919309	27.342927342927343	36.77703677703678	17.790842790842788
40	18.747318747318747	26.788151788151787	36.62298662298662	17.841542841542843
41	16.85874185874186	27.909402909402907	36.16473616473617	19.06711906711907
42	17.085917085917085	27.839202839202837	36.38508638508638	18.689793689793692
43	18.23739323739324	28.236028236028236	35.65578565578566	17.87079287079287
44	17.97219297219297	28.345228345228346	35.17706017706018	18.505518505518506
45	17.90881790881791	27.30880230880231	35.58948558948559	19.19289419289419
46	18.116493116493114	27.92402792402792	35.33306033306033	18.626418626418626
47	17.118092118092118	27.57887757887758	37.22456222456223	18.07846807846808
48	16.58964158964159	27.21715221715222	37.26453726453726	18.92866892866893
49	17.05861705861706	27.28345228345228	37.00616200616201	18.651768651768652
50	16.176241176241177	26.919776919776922	37.440037440037436	19.463944463944465
51	16.81096681096681	25.33832533832534	36.577161577161576	21.273546273546273
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	129.0
1	220.0
2	311.0
3	4895.0
4	9479.0
5	6224.5
6	2970.0
7	2460.5
8	1951.0
9	1801.0
10	1651.0
11	1559.0
12	1467.0
13	1372.0
14	1277.0
15	1185.5
16	1094.0
17	1074.0
18	1054.0
19	1038.5
20	1023.0
21	1093.5
22	1164.0
23	1194.5
24	1225.0
25	1423.5
26	1812.0
27	2002.0
28	2212.0
29	2422.0
30	2583.0
31	2744.0
32	3009.0
33	3274.0
34	3505.0
35	3736.0
36	3758.0
37	3780.0
38	3947.5
39	4115.0
40	4339.5
41	4564.0
42	4847.0
43	5130.0
44	5423.5
45	5717.0
46	6410.0
47	7103.0
48	6577.0
49	6051.0
50	5658.0
51	5265.0
52	4663.5
53	4062.0
54	3738.0
55	3414.0
56	3249.0
57	3084.0
58	2910.5
59	2737.0
60	2528.5
61	2320.0
62	2030.0
63	1740.0
64	1423.0
65	1106.0
66	882.5
67	659.0
68	556.0
69	453.0
70	364.5
71	276.0
72	209.0
73	142.0
74	126.5
75	86.0
76	61.0
77	45.0
78	29.0
79	20.5
80	12.0
81	17.0
82	22.0
83	15.0
84	8.0
85	6.0
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	102564.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.03611403611403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.22930052681264	51.37572637572637
2	10.243917293462042	13.119613119613119
3	4.244952647766375	8.154908154908155
4	2.2990955875635675	5.889005889005889
5	1.1982703492798197	3.8366288366288366
6	0.6562319193641707	2.5213525213525214
7	0.37150948567252357	1.6653016653016655
8	0.22381923931910228	1.1466011466011465
9	0.12028380888577607	0.6932256932256933
>10	0.3852127044063461	3.8541788541788544
>50	0.010658059015195348	0.4855504855504855
>100	0.01218063887450897	1.365001365001365
>500	0.0015225798593136212	0.7566007566007565
>1k	0.0030451597186272423	5.136305136305136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3295	3.212628212628213	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1973	1.9236769236769238	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	776	0.7566007566007565	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	356	0.3471003471003471	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG	185	0.18037518037518038	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	165	0.16087516087516088	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC	163	0.15892515892515893	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	145	0.14137514137514137	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG	143	0.13942513942513943	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC	136	0.1326001326001326	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC	107	0.10432510432510432	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.059475059475059475	0.0
2	0.0	0.0	0.0	0.24667524667524668	0.0
3	0.0	0.0	0.0	0.32565032565032564	0.0
4	0.0	0.0	0.0	0.42412542412542414	0.0
5	0.0	0.0	0.0	0.8102258102258102	0.0
6	0.0	0.0	0.0	1.014976014976015	0.0
7	0.0	0.0	0.0	1.2714012714012715	0.0
8	0.0	0.0	0.0	1.8525018525018524	0.0
9	0.0	0.0	0.0	2.0767520767520766	0.0
10	0.0	0.0	0.0	2.8782028782028783	0.0
11	0.0	0.0	0.0	3.495378495378495	0.0
12	0.0	0.0	0.0	4.27050427050427	0.0
13	0.0	0.0	0.0	4.515229515229516	0.0
14	0.0	0.0	0.0	4.633204633204633	0.0
15	0.0	0.0	0.0	4.8418548418548415	0.0
16	0.0	0.0	0.0	5.201630201630202	0.0
17	0.0	0.0	0.0	5.571155571155571	0.0
18	0.0	0.0	0.0	5.982605982605983	0.0
19	0.0	0.0	0.0	6.229281229281229	0.0
20	0.0	0.0	0.0	6.4603564603564605	0.0
21	0.0	0.0	0.0	6.775281775281775	0.0
22	0.0	0.0	0.0	7.051207051207051	0.0
23	0.0	0.0	0.0	7.39050739050739	0.0
24	0.0	0.0	0.0	7.663507663507663	0.0
25	0.0	0.0	0.0	7.923832923832924	0.0
26	0.0	0.0	0.0	8.151008151008151	0.0
27	0.0	0.0	0.0	8.403533403533404	0.0
28	0.0	0.0	0.0	8.662883662883663	0.0
29	0.0	0.0	0.0	8.938808938808938	0.0
30	0.0	0.0	0.0	9.222534222534222	0.0
31	0.0	0.0	0.0	9.492609492609493	0.0
32	0.0	0.0	0.0	9.75098475098475	0.0
33	0.0	0.0	0.0	10.013260013260012	0.0
34	0.0	0.0	0.0	10.278460278460278	0.0
35	0.0	0.0	0.0	10.561210561210562	0.0
36	0.0	0.0	0.0	10.844935844935845	0.0
37	0.0	0.0	0.0	11.1003861003861	0.0
38	0.0	0.0	0.0	11.425061425061426	0.0
39	0.0	0.0	0.0	11.721461721461722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	280	0.0	45.000004	1
TACGAAT	35	1.1975135E-7	45.000004	12
CGAATAT	35	1.1975135E-7	45.000004	14
GCTACGA	35	1.1975135E-7	45.000004	10
TGACTAT	35	1.1975135E-7	45.000004	23
CGTTGTT	20	7.0032396E-4	45.0	1
AGGCGAT	40	6.710252E-9	45.0	7
TATGCCA	20	7.0032396E-4	45.0	10
ACGGGCA	25	3.8659222E-5	45.0	5
AACGGGC	25	3.8659222E-5	45.0	4
CGGGGAT	20	7.0032396E-4	45.0	6
ATGCGGG	20	7.0032396E-4	45.0	3
TGAGGAC	20	7.0032396E-4	45.0	5
AGGATGC	40	6.710252E-9	45.0	7
AGGATAC	25	3.8659222E-5	45.0	7
TGGGAGA	25	3.8659222E-5	45.0	6
GCATAGG	20	7.0032396E-4	45.0	3
TCGCATT	30	2.1461492E-6	44.999996	22
CGCATTT	30	2.1461492E-6	44.999996	23
AGGACTA	30	2.1461492E-6	44.999996	7
>>END_MODULE