##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936177.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	672065
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.735808292352676	31.0	31.0	34.0	30.0	34.0
2	31.90134138810978	31.0	31.0	34.0	30.0	34.0
3	31.49534048045948	31.0	31.0	34.0	28.0	34.0
4	35.44981065819526	37.0	35.0	37.0	33.0	37.0
5	31.795870935103004	37.0	35.0	37.0	0.0	37.0
6	33.749653679331615	37.0	35.0	37.0	17.0	37.0
7	21.046153273864878	32.0	0.0	37.0	0.0	37.0
8	28.2213684688237	35.0	17.0	37.0	17.0	37.0
9	34.72263694731908	37.0	32.0	39.0	30.0	39.0
10	36.14219457939336	37.0	35.0	39.0	32.0	39.0
11	36.570284124303456	38.0	35.0	39.0	32.0	39.0
12	36.99718479611347	39.0	37.0	39.0	33.0	39.0
13	36.843195226652185	39.0	37.0	39.0	33.0	39.0
14	38.236542596326245	40.0	37.0	41.0	34.0	41.0
15	38.36635742078519	40.0	38.0	41.0	34.0	41.0
16	38.40242089678826	40.0	38.0	41.0	34.0	41.0
17	38.339889742807614	40.0	38.0	41.0	34.0	41.0
18	38.17863599503024	40.0	37.0	41.0	33.0	41.0
19	38.12222478480504	40.0	37.0	41.0	34.0	41.0
20	38.024045293238004	40.0	37.0	41.0	34.0	41.0
21	37.92133945377307	40.0	36.0	41.0	33.0	41.0
22	37.86624656841228	39.0	36.0	41.0	33.0	41.0
23	37.80724185904637	39.0	36.0	41.0	33.0	41.0
24	37.682899719521174	39.0	35.0	41.0	33.0	41.0
25	37.52308184476204	39.0	35.0	41.0	33.0	41.0
26	37.406869871217815	39.0	35.0	41.0	33.0	41.0
27	37.35075773920677	39.0	35.0	41.0	33.0	41.0
28	37.15669764085319	39.0	35.0	41.0	32.0	41.0
29	37.10549277227649	39.0	35.0	41.0	32.0	41.0
30	36.9495167878107	39.0	35.0	41.0	31.0	41.0
31	36.79701219376102	39.0	35.0	41.0	31.0	41.0
32	36.6980292084843	39.0	35.0	41.0	31.0	41.0
33	36.43137494141192	39.0	35.0	41.0	30.0	41.0
34	36.1616644223401	39.0	35.0	41.0	29.0	41.0
35	35.92084247803412	39.0	35.0	41.0	27.0	41.0
36	35.67309114445775	39.0	35.0	40.0	26.0	41.0
37	35.57289696681124	39.0	35.0	40.0	25.0	41.0
38	35.41660702461815	39.0	35.0	40.0	24.0	41.0
39	35.33346179313013	39.0	35.0	40.0	24.0	41.0
40	35.28701688080766	39.0	35.0	40.0	24.0	41.0
41	35.148734125419416	39.0	35.0	40.0	23.0	41.0
42	35.048996748826376	38.0	34.0	40.0	23.0	41.0
43	34.91018874662421	38.0	34.0	40.0	23.0	41.0
44	34.80931308727578	38.0	34.0	40.0	22.0	41.0
45	34.732494624775875	38.0	34.0	40.0	22.0	41.0
46	34.546771517635946	38.0	34.0	40.0	22.0	41.0
47	34.51058900552774	38.0	34.0	40.0	22.0	41.0
48	34.39347235758446	38.0	33.0	40.0	20.0	41.0
49	34.19904175935363	38.0	33.0	40.0	20.0	41.0
50	34.006764226674505	38.0	33.0	40.0	19.0	41.0
51	32.0714335666937	36.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	23.0
10	23.0
11	14.0
12	12.0
13	6.0
14	11.0
15	21.0
16	60.0
17	115.0
18	253.0
19	586.0
20	1135.0
21	1904.0
22	3059.0
23	4559.0
24	6959.0
25	9624.0
26	12190.0
27	12799.0
28	12967.0
29	13511.0
30	15209.0
31	18491.0
32	24135.0
33	33400.0
34	44989.0
35	57794.0
36	73669.0
37	118491.0
38	124409.0
39	81623.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.80145521638532	18.59224926160416	24.840752010594215	17.765543511416308
2	34.0245363171717	24.2729497890829	25.069003742197555	16.633510151547842
3	28.365708674012186	23.734162618199132	32.06698756816677	15.833141139621912
4	25.688140284049904	24.05243540431357	32.634938584809504	17.624485726827018
5	21.59210790622931	34.89632699218082	27.370120449658884	16.14144465193099
6	24.031752881045733	32.71632952169805	30.05497980106091	13.196937796195307
7	45.94049682694382	41.81009277376444	9.392990261358648	2.856420137933087
8	78.06164582294868	5.070491693511788	13.257497414684591	3.610365068854947
9	72.01267734519726	7.822755239448566	15.052264289912435	5.112303125441735
10	34.11039110800294	33.54407683780587	20.65826966141668	11.687262392774509
11	23.846056557029456	25.36823075148981	34.08182244276967	16.703890248711062
12	23.815553555087675	21.705638591505284	36.19798680187184	18.28082105153519
13	21.766793390520263	23.678959624441088	36.71713301540774	17.837113969630913
14	18.08679220015921	27.166717505003234	34.42509281096323	20.32139748387433
15	17.47673216132368	25.74319448267653	38.81469798308199	17.965375372917798
16	20.577771495316675	26.301622610908172	35.07934500383148	18.04126088994368
17	20.97817919397677	24.13397513633354	33.661029811104584	21.22681585858511
18	21.580650681109713	24.63050448989309	34.310074174373014	19.47877065462418
19	22.01795957236279	26.031261857112035	32.32678386763185	19.62399470289332
20	22.67027742852254	25.96698236033717	32.85515537931599	18.507584831824303
21	22.302158273381295	26.53404060619137	33.30466547134578	17.859135649081562
22	20.966870763988602	24.44689129771674	33.50330697179588	21.082930966498775
23	19.629053737361712	25.848243845461376	34.19788264527985	20.32481977189706
24	19.964735553852677	24.312082908647227	34.96745106500116	20.75573047249894
25	19.674287457314396	26.414558115658455	32.80843370804907	21.102720718978073
26	19.619679644082048	26.325727422198746	32.506082000996926	21.54851093272228
27	20.012498791039558	26.07292449391056	32.97716738708309	20.937409327966787
28	18.593588417786968	25.946746222463602	34.76270896416269	20.696956395586735
29	19.51939172550274	24.66785206788034	34.52954699322238	21.28320921339454
30	20.81390936888545	24.99877244016576	33.41760097609606	20.76971721485273
31	20.5383407854895	26.170980485518513	32.647734966111905	20.64294376288008
32	21.185302016918005	26.025607642117947	33.11182698102118	19.677263359942863
33	20.448022140715555	25.26481813515062	32.644015087826325	21.6431446363075
34	19.34842611949737	25.05754651707796	33.92811707200941	21.665910291415265
35	19.134458720510665	25.456317469292404	33.29469619754042	22.114527612656516
36	20.063386725986327	27.223110859812667	32.29092424095884	20.42257817324217
37	19.378482736044877	27.885397989777776	32.927618608319136	19.808500665858215
38	19.47281884936725	27.474872222180892	31.621643739816836	21.43066518863503
39	20.552625118106135	26.288231049080075	31.629232291519422	21.529911541294368
40	21.66918378430658	24.807570696286817	32.12219056192481	21.40105495748179
41	18.887756392610832	26.707982114825203	31.446363075000182	22.957898417563776
42	20.801856963240162	26.409945466584333	31.19594086881477	21.592256701360732
43	20.67686905284459	26.08765521192147	31.276736625177627	21.958739110056317
44	19.74883381815747	26.043760648151594	31.412140194772824	22.79526533891811
45	20.00669578091405	25.586215619024944	30.75178740151622	23.655301198544784
46	20.632676898811873	26.664533936449597	29.966446697864047	22.736342466874486
47	19.297686979682023	25.932908275241235	31.9854478361468	22.78395690892994
48	19.3479797341031	25.181344066422145	32.71677590709232	22.753900292382433
49	19.892718710243802	25.15887600157723	32.41769769293148	22.530707595247485
50	18.96572504147664	24.80638033523543	32.838787914859424	23.3891067084285
51	19.118835231711216	24.533192473942254	31.591884713532174	24.756087580814356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	323.0
1	730.0
2	1137.0
3	10187.5
4	19238.0
5	13054.5
6	6871.0
7	5883.5
8	4896.0
9	4658.5
10	4421.0
11	4286.5
12	4152.0
13	3961.5
14	3771.0
15	3645.0
16	3519.0
17	3463.0
18	3407.0
19	3514.0
20	3621.0
21	3957.0
22	4293.0
23	4725.5
24	5158.0
25	5971.5
26	7955.5
27	9126.0
28	10556.0
29	11986.0
30	13627.0
31	15268.0
32	17666.5
33	20065.0
34	22579.5
35	25094.0
36	26513.5
37	27933.0
38	29890.0
39	31847.0
40	34186.5
41	36526.0
42	38967.5
43	41409.0
44	45472.0
45	49535.0
46	54613.5
47	59692.0
48	56473.0
49	53254.0
50	50425.5
51	47597.0
52	42024.0
53	36451.0
54	32959.5
55	29468.0
56	27690.5
57	25913.0
58	24034.5
59	22156.0
60	20433.0
61	18710.0
62	16605.0
63	14500.0
64	12040.5
65	9581.0
66	7716.0
67	5851.0
68	4606.5
69	3362.0
70	2727.0
71	2092.0
72	1695.0
73	1298.0
74	1036.0
75	626.5
76	479.0
77	343.5
78	208.0
79	177.0
80	146.0
81	105.5
82	65.0
83	55.0
84	45.0
85	32.5
86	20.0
87	16.5
88	13.0
89	8.0
90	3.0
91	2.0
92	1.0
93	2.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	672065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.261989677682735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81105869368149	38.03578501161704
2	7.834586354536692	7.5622545154312775
3	3.082563393165461	4.4631192798526245
4	1.8371775141942066	3.546633689044465
5	1.3927249692680157	3.360783904533199
6	1.101138708750419	3.1885886997245767
7	0.8911054205579801	3.010456442608746
8	0.7474323154571527	2.885805655468769
9	0.637126072751845	2.7674074751878877
>10	3.6240449737100713	26.43705868567284
>50	0.02388485666751779	0.8064244170150359
>100	0.014801882341273153	1.542061373790917
>500	0.0013456256673884572	0.48447158763866893
>1k	6.728128336942286E-4	0.8030149036396973
>5k	3.364064168471143E-4	1.106134358774213
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6813	1.0137412303869417	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3683	0.5480124690320133	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	1263	0.18792825098762767	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG	880	0.13093971565250384	No Hit
GAATCTATCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	821	0.12216080289852917	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	718	0.10683490436192927	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4879513142329983E-4	0.0	0.0	0.06680901400906163	0.0
2	1.4879513142329983E-4	0.0	0.0	0.3035420681035316	0.0
3	1.4879513142329983E-4	0.0	0.0	0.4157335971966997	0.0
4	1.4879513142329983E-4	0.0	0.0	0.5859552275449548	0.0
5	1.4879513142329983E-4	0.0	0.0	1.1094164998921234	0.0
6	1.4879513142329983E-4	0.0	0.0	1.56963984138439	0.0
7	1.4879513142329983E-4	0.0	0.0	1.9410324894169462	0.0
8	1.4879513142329983E-4	0.0	0.0	2.782468957615707	0.0
9	1.4879513142329983E-4	0.0	0.0	3.1203827010780207	0.0
10	1.4879513142329983E-4	0.0	0.0	3.9804185607046936	0.0
11	1.4879513142329983E-4	0.0	0.0	4.696420733113612	0.0
12	1.4879513142329983E-4	0.0	0.0	5.445306629567081	0.0
13	1.4879513142329983E-4	0.0	0.0	5.827858912456384	0.0
14	1.4879513142329983E-4	0.0	0.0	6.006264275032921	0.0
15	1.4879513142329983E-4	0.0	0.0	6.235111187161956	0.0
16	1.4879513142329983E-4	0.0	0.0	6.620044192154032	0.0
17	1.4879513142329983E-4	0.0	0.0	7.115531979793621	0.0
18	1.4879513142329983E-4	0.0	0.0	7.707439012595508	0.0
19	1.4879513142329983E-4	0.0	0.0	8.039549745932312	0.0
20	1.4879513142329983E-4	0.0	0.0	8.372999635451928	0.0
21	1.4879513142329983E-4	0.0	0.0	8.771175407140678	0.0
22	1.4879513142329983E-4	0.0	0.0	9.195687917091353	0.0
23	1.4879513142329983E-4	0.0	0.0	9.629276930058849	0.0
24	1.4879513142329983E-4	0.0	0.0	9.964214770892696	0.0
25	1.4879513142329983E-4	0.0	0.0	10.274899005304546	0.0
26	1.4879513142329983E-4	0.0	0.0	10.569810955785528	0.0
27	1.4879513142329983E-4	0.0	0.0	10.865169291660777	0.0
28	1.4879513142329983E-4	0.0	0.0	11.184334848563754	0.0
29	1.4879513142329983E-4	0.0	0.0	11.53444979280278	0.0
30	1.4879513142329983E-4	0.0	0.0	11.931583998571567	0.0
31	1.4879513142329983E-4	0.0	0.0	12.306101344364013	0.0
32	1.4879513142329983E-4	0.0	0.0	12.656067493471614	0.0
33	1.4879513142329983E-4	0.0	0.0	13.003206535082173	0.0
34	1.4879513142329983E-4	0.0	0.0	13.376831110086078	0.0
35	1.4879513142329983E-4	0.0	0.0	13.748967733775752	0.0
36	1.4879513142329983E-4	0.0	0.0	14.114854961945644	0.0
37	1.4879513142329983E-4	0.0	0.0	14.459464486322007	0.0
38	1.4879513142329983E-4	0.0	0.0	14.817316777395044	0.0
39	2.9759026284659966E-4	0.0	0.0	15.198381108970114	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	30	2.1641517E-6	44.999996	2
ACGGTTA	25	3.8890714E-5	44.999996	34
CGTTTTA	1505	0.0	44.102993	1
CGTTATT	580	0.0	43.83621	1
CGTTTTT	2705	0.0	42.338264	1
TGGGCGA	230	0.0	42.065216	6
ATGGGCG	105	0.0	40.714287	5
GACCGAT	290	0.0	38.793106	9
CGAAGGA	180	0.0	38.75	4
ACCGATC	35	6.244416E-6	38.57143	10
GCCGATC	35	6.244416E-6	38.57143	9
ACATACG	135	0.0	38.333336	17
AGGCGAT	370	0.0	38.310814	7
AACGAGC	200	0.0	38.249996	15
TAGGCGA	130	0.0	38.07692	6
CCTTATC	95	0.0	37.894733	43
GCGATGT	125	0.0	37.8	9
CGGTTTA	155	0.0	37.74194	1
CGTATTG	30	1.1394175E-4	37.499996	13
CCGTAGA	30	1.1394175E-4	37.499996	1
>>END_MODULE