##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936176.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	170568
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.711534402701563	31.0	31.0	34.0	30.0	34.0
2	31.887868767881432	31.0	31.0	34.0	30.0	34.0
3	31.618509919797383	31.0	31.0	34.0	30.0	34.0
4	35.50816096805966	37.0	35.0	37.0	33.0	37.0
5	31.91277965386239	37.0	35.0	37.0	10.0	37.0
6	33.78637259040383	37.0	35.0	37.0	19.0	37.0
7	20.858484592655127	32.0	0.0	37.0	0.0	37.0
8	28.047230430092398	35.0	17.0	37.0	17.0	37.0
9	34.550449087753854	35.0	32.0	39.0	28.0	39.0
10	36.04847919891187	37.0	35.0	39.0	32.0	39.0
11	36.49427207917077	37.0	35.0	39.0	32.0	39.0
12	36.8563798602317	39.0	35.0	39.0	33.0	39.0
13	36.63161906101965	39.0	35.0	39.0	32.0	39.0
14	37.96687538107969	40.0	37.0	41.0	33.0	41.0
15	38.091851930022045	40.0	37.0	41.0	33.0	41.0
16	38.18296515172834	40.0	37.0	41.0	33.0	41.0
17	38.10307326110408	40.0	37.0	41.0	33.0	41.0
18	37.99030298766474	40.0	37.0	41.0	33.0	41.0
19	37.89009075559308	40.0	37.0	41.0	33.0	41.0
20	37.85146100089114	40.0	36.0	41.0	33.0	41.0
21	37.80467614089395	39.0	36.0	41.0	33.0	41.0
22	37.75761573096947	39.0	36.0	41.0	33.0	41.0
23	37.717883776558324	39.0	36.0	41.0	33.0	41.0
24	37.57163711833404	39.0	35.0	41.0	33.0	41.0
25	37.423057079874305	39.0	35.0	41.0	33.0	41.0
26	37.26754139111674	39.0	35.0	41.0	32.0	41.0
27	37.2573577693354	39.0	35.0	41.0	33.0	41.0
28	37.11128113127902	39.0	35.0	41.0	32.0	41.0
29	37.10779864921908	39.0	35.0	41.0	32.0	41.0
30	36.949920266404014	39.0	35.0	40.0	31.0	41.0
31	36.71980090052061	39.0	35.0	40.0	31.0	41.0
32	36.61529712490033	39.0	35.0	40.0	31.0	41.0
33	36.4121816518925	39.0	35.0	40.0	30.0	41.0
34	36.15209183434173	39.0	35.0	40.0	30.0	41.0
35	35.967625814924254	39.0	35.0	40.0	29.0	41.0
36	35.683146897425075	38.0	35.0	40.0	27.0	41.0
37	35.608883260635054	38.0	35.0	40.0	27.0	41.0
38	35.425718774916746	38.0	35.0	40.0	26.0	41.0
39	35.416742882604005	38.0	35.0	40.0	26.0	41.0
40	35.310808592467524	38.0	34.0	40.0	25.0	41.0
41	35.174616575207544	38.0	34.0	40.0	24.0	41.0
42	35.067626987477134	38.0	34.0	40.0	24.0	41.0
43	34.981362271938465	38.0	34.0	40.0	24.0	41.0
44	34.831633131654236	38.0	34.0	40.0	23.0	41.0
45	34.71901529008958	38.0	34.0	40.0	23.0	41.0
46	34.53376366024108	38.0	33.0	40.0	23.0	41.0
47	34.450770367243564	38.0	33.0	40.0	23.0	41.0
48	34.28301908916092	37.0	33.0	40.0	22.0	41.0
49	34.1883237183997	37.0	33.0	40.0	22.0	41.0
50	33.99239599455935	37.0	33.0	40.0	20.0	41.0
51	31.948706674171007	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	10.0
10	5.0
11	5.0
12	3.0
13	3.0
14	7.0
15	10.0
16	23.0
17	38.0
18	72.0
19	109.0
20	224.0
21	368.0
22	614.0
23	988.0
24	1525.0
25	2105.0
26	2646.0
27	2874.0
28	3222.0
29	3577.0
30	4378.0
31	5369.0
32	6947.0
33	9900.0
34	12803.0
35	16357.0
36	19622.0
37	29904.0
38	28855.0
39	17995.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56348201303879	19.04519018807748	23.64628769757516	15.745040101308568
2	32.97453215140003	26.86142770038929	23.532550068008067	16.631490080202617
3	29.297992589465782	25.823718399699825	29.368345762393883	15.509943248440505
4	26.13092725481919	24.43482951081094	31.72634960836734	17.707893626002534
5	22.884128324187422	34.95321514000281	25.226302706252053	16.936353829557714
6	23.761784156465456	34.567445241780405	28.383987617841566	13.286782983912573
7	44.822592748932976	42.46752028516486	9.705220205431266	3.004666760470897
8	75.67128652502227	6.736316307865485	13.238122039303974	4.354275127808265
9	69.88649688100934	7.132052905586042	14.120468083110548	8.860982130294076
10	37.43785469724685	26.693752638243986	21.539210168378595	14.329182496130574
11	28.154167252943108	24.43951972233948	29.44866563482013	17.957647389897286
12	26.490900989634632	22.04458046057877	33.64464612354017	17.819872426246423
13	22.331269640260775	24.43893344589841	35.840837671779	17.388959242061816
14	18.7725716429811	27.715046198583554	32.92118099526289	20.59120116317246
15	17.176727170395385	24.80828760377093	39.68505229585854	18.329932929975143
16	21.14288729421697	24.421931429107453	33.99992964682707	20.435251629848505
17	21.188030580179166	23.52727358003846	32.87721026218283	22.40748557759955
18	22.0873786407767	25.077974766661974	33.12637774963651	19.708268842924817
19	22.052788330753717	26.16669011772431	31.144763378828383	20.63575817269359
20	22.985554148492096	26.36074761971765	32.015970170254676	18.637728061535576
21	22.263847849538017	27.503400403358192	31.92451104544815	18.308240701655645
22	21.546245485671403	23.8133764832794	31.50825477229023	23.13212325875897
23	20.71021528070916	26.344918155808827	32.03297218704563	20.911894376436376
24	21.560902396698093	24.7619717649266	32.721847005299935	20.955278833075372
25	20.517916608039023	27.471155199099478	31.396862248487405	20.61406594437409
26	20.58651095164392	25.606209840063787	31.497115519909947	22.310163688382346
27	21.463580507480888	26.06878195206604	31.666549411378455	20.80108812907462
28	17.467520285164863	25.860653815487076	35.23111017306881	21.440715726279254
29	22.530603630223723	24.678720510295012	31.738075137188687	21.052600722292574
30	21.24489939496271	25.79030064255898	32.734158810562356	20.23064115191595
31	22.449111204915344	26.081680033769523	30.66694807935838	20.802260681956756
32	22.847779184841237	26.526663852539752	29.456873504995073	21.16868345762394
33	21.030908493973076	24.682824445382487	30.33746071947845	23.948806341165987
34	21.28769757516064	26.249941372355895	31.168214436471086	21.294146616012384
35	21.7121617184935	26.135617466347732	29.520777637071433	22.63144317808733
36	23.145021340462453	26.344918155808827	31.143004549505182	19.367055954223535
37	20.90661788846677	27.005651704891893	31.449040851742417	20.638689554898924
38	19.58808217250598	27.694526523146195	30.279419351812763	22.43797195253506
39	21.092467520285165	26.350780920219503	30.171544486656348	22.385207072838988
40	21.40905679846161	26.731860606913372	30.607147882369496	21.251934712255522
41	19.444444444444446	27.324586088832607	31.33354439285212	21.89742507387083
42	20.36196707471507	26.204211809952625	31.51294498381877	21.92087613151353
43	21.557971014492754	25.136602410768727	30.38377655832278	22.92165001641574
44	20.657450401013087	25.249167487453683	30.518620139768306	23.574761971764925
45	20.788776323812204	25.97321889217204	28.870597063927583	24.367407720088178
46	23.102809436705595	27.389662773791095	28.572182355424232	20.93534543407908
47	19.86890858777731	25.85537732751747	33.12696402607757	21.148750058627645
48	21.0701890155246	26.251700201679096	30.605389053046295	22.07272172975001
49	20.29395900755124	23.957600487782	32.781060925847754	22.967379578819006
50	19.950987289526758	24.556775010552975	32.34956146522208	23.142676234698182
51	20.3127198536654	24.72210496693401	29.569438581680036	25.395736597720557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	194.0
1	304.0
2	414.0
3	2052.0
4	3690.0
5	2500.5
6	1311.0
7	1156.0
8	1001.0
9	984.0
10	967.0
11	930.5
12	894.0
13	886.5
14	879.0
15	882.0
16	885.0
17	849.5
18	814.0
19	780.5
20	747.0
21	831.5
22	916.0
23	1065.5
24	1215.0
25	1425.0
26	1914.0
27	2193.0
28	2557.5
29	2922.0
30	3351.5
31	3781.0
32	4287.5
33	4794.0
34	5228.0
35	5662.0
36	6024.0
37	6386.0
38	6962.0
39	7538.0
40	8058.5
41	8579.0
42	9302.5
43	10026.0
44	11377.5
45	12729.0
46	14882.5
47	17036.0
48	15218.0
49	13400.0
50	12668.5
51	11937.0
52	10610.5
53	9284.0
54	8545.5
55	7807.0
56	7421.0
57	7035.0
58	6533.0
59	6031.0
60	5558.0
61	5085.0
62	4567.5
63	4050.0
64	3582.5
65	3115.0
66	2612.5
67	2110.0
68	1733.0
69	1356.0
70	1101.5
71	847.0
72	696.5
73	546.0
74	439.5
75	262.5
76	192.0
77	150.0
78	108.0
79	85.5
80	63.0
81	44.5
82	26.0
83	20.5
84	15.0
85	12.0
86	9.0
87	8.0
88	7.0
89	4.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	170568.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.37655363256882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.40300164961347	42.632850241545896
2	8.431357750487877	9.169363538295578
3	3.738045693215021	6.097861263542986
4	2.3310224368995893	5.070118662351672
5	1.6205026469288077	4.405867454622204
6	1.2582345901303518	4.10510764035458
7	0.9865335475315098	3.755100605037287
8	0.7590378332920031	3.301908916092116
9	0.5746692686713604	2.8123680878007598
>10	1.8609365060539735	13.608648750058627
>50	0.015094502366602336	0.5868627175085597
>100	0.01401632362613074	1.361333896158717
>500	0.006469072442829572	2.327517471037944
>1k	0.0010781787404715954	0.7650907555930773
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1305	0.7650907555930773	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCG	857	0.5024389099948408	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTC	762	0.446742648093429	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	734	0.43032690774353927	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCG	601	0.3523521410815628	No Hit
GAATCTATCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTC	512	0.300173537826556	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGC	504	0.29548332629801605	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGC	351	0.20578303081468974	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	263	0.15419070400075044	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCT	224	0.13132592279911823	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	193	0.11315135312602598	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCG	177	0.10377093006894611	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06449040851742413	0.0
2	0.0	0.0	0.0	0.4385347779184841	0.0
3	0.0	0.0	0.0	0.5481684723981052	0.0
4	0.0	0.0	0.0	0.7832653252661695	0.0
5	0.0	0.0	0.0	1.7342057126776418	0.0
6	0.0	0.0	0.0	2.0197223394775103	0.0
7	0.0	0.0	0.0	2.4893297687725715	0.0
8	0.0	0.0	0.0	3.345879649172178	0.0
9	0.0	0.0	0.0	3.6079452183293466	0.0
10	0.0	0.0	0.0	5.314009661835748	0.0
11	0.0	0.0	0.0	6.220979316167159	0.0
12	0.0	0.0	0.0	7.581140659443741	0.0
13	0.0	0.0	0.0	7.958116411050138	0.0
14	0.0	0.0	0.0	8.110548285727686	0.0
15	0.0	0.0	0.0	8.455278833075372	0.0
16	0.0	0.0	0.0	9.004619858355612	0.0
17	0.0	0.0	0.0	9.605553210449791	0.0
18	0.0	0.0	0.0	10.292082922939825	0.0
19	0.0	0.0	0.0	10.684301862013976	0.0
20	0.0	0.0	0.0	11.084142394822006	0.0
21	0.0	0.0	0.0	11.547887059706392	0.0
22	0.0	0.0	0.0	12.052084799024437	0.0
23	0.0	0.0	0.0	12.53283148069978	0.0
24	0.0	0.0	0.0	12.920946484686459	0.0
25	0.0	0.0	0.0	13.301439894939262	0.0
26	0.0	0.0	0.0	13.61040757938183	0.0
27	0.0	0.0	0.0	13.958069508934853	0.0
28	0.0	0.0	0.0	14.322733455278833	0.0
29	0.0	0.0	0.0	14.673326767037194	0.0
30	0.0	0.0	0.0	15.063200600347075	0.0
31	0.0	0.0	0.0	15.420242952957178	0.0
32	0.0	0.0	0.0	15.779044134890484	0.0
33	0.0	0.0	0.0	16.13960414614699	0.0
34	0.0	0.0	0.0	16.52127010928193	0.0
35	0.0	0.0	0.0	16.901763519534732	0.0
36	0.0	0.0	0.0	17.27522161249472	0.0
37	0.0	0.0	0.0	17.621124712724544	0.0
38	0.0	0.0	0.0	17.989306317714927	0.0
39	0.0	0.0	0.0	18.380352703906947	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTTGG	25	3.8767994E-5	45.000004	2
GGCCGGT	25	3.8767994E-5	45.000004	9
ACTTAGG	25	3.8767994E-5	45.000004	3
CATACGA	30	2.1546039E-6	45.000004	18
TAAGGCA	35	1.2038072E-7	45.000004	5
ACATACG	30	2.1546039E-6	45.000004	17
CTAGGAT	25	3.8767994E-5	45.000004	5
TAATACG	45	3.8016879E-10	45.0	4
GTACACT	20	7.0163823E-4	45.0	18
CCATCTA	20	7.0163823E-4	45.0	13
CATAGCC	20	7.0163823E-4	45.0	36
CTACGAA	20	7.0163823E-4	45.0	11
TACGAAT	20	7.0163823E-4	45.0	12
GCCTAAG	20	7.0163823E-4	45.0	2
TCATCGA	20	7.0163823E-4	45.0	16
TAGGCTC	20	7.0163823E-4	45.0	6
CGATGCG	20	7.0163823E-4	45.0	10
GCTACGA	20	7.0163823E-4	45.0	10
ATACGTC	20	7.0163823E-4	45.0	6
TGTACAC	20	7.0163823E-4	45.0	17
>>END_MODULE