##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936175.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	65623
Sequences flagged as poor quality	0
Sequence length	51
%GC	32
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262194657360986	31.0	31.0	33.0	28.0	34.0
2	31.411654450421345	31.0	31.0	34.0	28.0	34.0
3	29.862852963137925	31.0	30.0	34.0	25.0	34.0
4	34.496137025128384	35.0	35.0	37.0	30.0	37.0
5	31.249790469804793	35.0	35.0	37.0	0.0	37.0
6	33.43201316611554	35.0	35.0	37.0	17.0	37.0
7	21.79601664050714	32.0	0.0	37.0	0.0	37.0
8	28.65768099599226	35.0	17.0	37.0	17.0	37.0
9	34.956341526598905	37.0	32.0	39.0	31.0	39.0
10	36.350654496137025	37.0	35.0	39.0	32.0	39.0
11	36.77771513036588	39.0	37.0	39.0	33.0	39.0
12	37.222315346753426	39.0	37.0	39.0	34.0	39.0
13	37.141429072124104	39.0	37.0	39.0	34.0	39.0
14	38.64544443259223	40.0	38.0	41.0	35.0	41.0
15	38.75549731039422	40.0	38.0	41.0	35.0	41.0
16	38.75685354220319	40.0	38.0	41.0	35.0	41.0
17	38.71487131036375	40.0	38.0	41.0	35.0	41.0
18	38.39077762369901	40.0	38.0	41.0	34.0	41.0
19	38.055117870258904	39.0	37.0	41.0	34.0	41.0
20	37.69492403578014	39.0	35.0	40.0	34.0	41.0
21	37.53063712417902	39.0	35.0	40.0	33.0	41.0
22	37.30960181643631	39.0	35.0	40.0	33.0	41.0
23	37.10429270225378	38.0	35.0	40.0	33.0	41.0
24	36.79597092482818	38.0	35.0	40.0	33.0	41.0
25	36.64498727580269	38.0	35.0	40.0	33.0	41.0
26	36.53673254803956	38.0	35.0	40.0	33.0	41.0
27	36.44642884354571	38.0	35.0	40.0	33.0	41.0
28	36.361900553159714	38.0	35.0	40.0	32.0	41.0
29	36.340078935738994	38.0	35.0	40.0	32.0	41.0
30	36.1424957712997	38.0	35.0	40.0	31.0	41.0
31	35.854456516770036	37.0	34.0	40.0	30.0	41.0
32	35.473096322935554	37.0	34.0	40.0	29.0	41.0
33	34.712829343370466	37.0	33.0	40.0	24.0	41.0
34	33.77617603584109	37.0	33.0	40.0	18.0	41.0
35	32.81288572604117	37.0	32.0	40.0	14.0	41.0
36	32.08728646968289	37.0	31.0	40.0	9.0	41.0
37	31.641741462596954	37.0	29.0	40.0	8.0	41.0
38	31.3060969477165	37.0	26.0	40.0	7.0	41.0
39	31.123950444204013	37.0	25.0	40.0	7.0	41.0
40	31.036740167319383	37.0	25.0	40.0	7.0	41.0
41	30.928653063712417	37.0	24.0	40.0	7.0	41.0
42	30.72357252792466	36.0	23.0	40.0	7.0	41.0
43	30.55832558706551	36.0	23.0	40.0	7.0	41.0
44	30.398457857763283	36.0	23.0	40.0	7.0	41.0
45	30.322676500617163	36.0	23.0	40.0	7.0	41.0
46	30.122533258156437	35.0	22.0	40.0	7.0	41.0
47	30.036968745714155	35.0	22.0	40.0	7.0	41.0
48	29.872986605306068	35.0	21.0	40.0	7.0	41.0
49	29.70597199152736	35.0	20.0	40.0	7.0	41.0
50	29.416987946299315	35.0	20.0	40.0	7.0	41.0
51	28.017021471130548	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	0.0
11	1.0
12	0.0
13	0.0
14	2.0
15	4.0
16	10.0
17	13.0
18	38.0
19	98.0
20	157.0
21	313.0
22	530.0
23	981.0
24	1666.0
25	2644.0
26	3120.0
27	3058.0
28	2586.0
29	2195.0
30	1894.0
31	1909.0
32	2288.0
33	3044.0
34	3729.0
35	4935.0
36	6041.0
37	9032.0
38	9230.0
39	6101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.084314950550873	15.672858601404997	19.686695213568413	35.55613123447572
2	49.63960806424576	19.54650046477607	19.147250201910914	11.666641269067247
3	22.975176386327963	19.4809746582753	46.37093701903296	11.172911936363775
4	18.543803239717782	19.945750727641222	48.76186702832848	12.748579004312512
5	16.17420721393414	31.10037639242339	41.08620453194764	11.639211861694832
6	17.155570455480547	26.40385230787986	46.675708212059796	9.764869024579797
7	34.47266964326532	39.249958093960956	23.865108269966324	2.4122639928073997
8	58.65626380994469	4.801670146137787	33.39073190802006	3.1513341358974754
9	54.42756350669735	6.793349892568154	34.54276701766149	4.2363195830730085
10	24.241500693354464	26.31242094997181	40.15208082532039	9.293997531353336
11	16.395166328878595	21.673803392103377	49.040732669948035	12.89029760906999
12	15.141032869573168	19.956417719397162	51.24879996342746	13.653749447602213
13	14.885025067430627	20.297761455587217	52.04120506529723	12.776008411684927
14	13.201164225957363	22.226963107447084	50.610304314036235	13.961568352559317
15	12.971061975222101	23.205278637063227	51.50328390960487	12.320375478109808
16	15.348277280831416	22.61249866662603	49.4003626777197	12.638861374822852
17	15.293418466086587	22.460113070112612	48.548527193209694	13.697941270591105
18	14.93378845831492	23.368331225332582	49.1458787315423	12.552001584810204
19	14.341008487877726	24.800755832558707	47.88564984837633	12.972585831187237
20	14.275482681376955	24.438078112856772	48.54547948127943	12.740959724486842
21	14.633588833183486	24.035780138061348	48.806058851317374	12.52457217743779
22	13.995093183792267	21.788092589488443	49.28607348033464	14.930740746384652
23	12.949727991710224	23.75081907258126	49.00568398274995	14.293768952958565
24	13.586699785136307	23.098608719503833	49.13673559575149	14.177955899608369
25	13.493744571263125	24.649894092010424	47.43763619462689	14.41872514209957
26	13.793944196394555	24.58741599743992	47.69516785273456	13.92347195343096
27	13.615653048473858	24.538652606555626	48.121847522972125	13.723846821998384
28	12.913155448547004	24.17749874281883	48.457095835301644	14.45224997333252
29	14.438535269646312	23.11232342319004	47.330966277067496	15.118175030096156
30	14.600064001950535	24.41217256144949	46.756472578211905	14.231290858388066
31	14.261767977690749	25.678496868475992	45.909208661597305	14.150526492235954
32	14.732639470917208	25.48344330493882	45.59072276488426	14.193194459259711
33	13.684226566904897	26.274324550843453	44.542309860871946	15.4991390213797
34	13.6308916081252	26.43280557121741	44.79069838318882	15.14560443746857
35	14.534538195449768	27.49188546698566	41.94718315224845	16.026393185316124
36	15.346753424866282	29.206223427761607	40.48123371378937	14.965789433582739
37	14.67473294424211	30.131203998598053	39.98140895722536	15.212654099934475
38	15.595141947183153	29.08279109458574	38.465172271916856	16.85689468631425
39	15.954771954954818	29.006598296329035	38.088779848528716	16.949849900187434
40	16.96051689194337	28.529631379242034	38.382884049799614	16.12696767901498
41	16.655745698916537	29.293083217774257	37.5219054294988	16.529265653810402
42	17.303384484098565	29.436325678496868	37.784008655501886	15.476281181902687
43	16.710604513661366	29.052313975283056	37.41066394404401	16.826417567011568
44	16.453072855553692	28.986788168782283	36.773692150617926	17.786446825046095
45	16.48050226292611	27.664081191045824	37.03274766469073	18.822668881337336
46	16.87822866982613	28.979168888956615	36.281486673879584	17.861115767337672
47	16.04620331286287	28.887737531048565	37.60419365161604	17.461865504472517
48	16.661841122777073	28.26143272937842	36.64568824954665	18.431037898297852
49	16.67555582646328	27.661033479115556	36.833122533258155	18.830288161163004
50	15.212654099934475	27.610746232266127	37.078463343644756	20.09813632415464
51	15.7399082638709	26.964631303049234	36.316535361077676	20.978925072002195
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	84.0
1	143.5
2	203.0
3	3130.5
4	6058.0
5	4289.0
6	2520.0
7	2161.5
8	1803.0
9	1702.0
10	1601.0
11	1580.0
12	1559.0
13	1511.0
14	1463.0
15	1392.0
16	1321.0
17	1232.5
18	1144.0
19	1146.5
20	1149.0
21	1083.5
22	1018.0
23	1002.5
24	987.0
25	1049.0
26	1185.0
27	1259.0
28	1351.0
29	1443.0
30	1620.5
31	1798.0
32	1918.5
33	2039.0
34	2192.5
35	2346.0
36	2487.0
37	2628.0
38	2715.5
39	2803.0
40	2885.0
41	2967.0
42	3146.0
43	3325.0
44	3681.5
45	4038.0
46	4415.5
47	4793.0
48	4486.5
49	4180.0
50	3827.0
51	3474.0
52	2835.0
53	2196.0
54	1774.5
55	1353.0
56	1182.0
57	1011.0
58	868.0
59	725.0
60	623.5
61	522.0
62	422.5
63	323.0
64	265.0
65	207.0
66	141.5
67	76.0
68	61.0
69	46.0
70	31.5
71	17.0
72	17.0
73	17.0
74	10.5
75	4.0
76	4.0
77	3.5
78	3.0
79	2.0
80	1.0
81	1.5
82	2.0
83	1.0
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	65623.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.64268625329534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.09202441628558	66.63517364338722
2	7.735852857632104	11.703213812230468
3	2.324784947319648	5.275589351294515
4	0.8662543564535949	2.621032260030782
5	0.3485162875964463	1.3181354098410618
6	0.1772799613207357	0.8045959495908447
7	0.09871270573540965	0.5226825960410222
8	0.05439271540522573	0.3291528884689819
9	0.04834908036020065	0.3291528884689819
>10	0.22361449666592803	2.9699952760465083
>50	0.020145450150083604	0.9585054020693965
>100	0.006043635045025081	1.2663243070264998
>500	0.0	0.0
>1k	0.004029090030016721	5.26644621550371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2207	3.363150115051125	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1249	1.9032961004525852	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	470	0.7162123036130624	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	259	0.3946786949697515	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102	0.15543330844368589	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC	85	0.12952775703640493	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCC	68	0.10362220562912393	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGC	68	0.10362220562912393	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09600292580345306	0.0
2	0.0	0.0	0.0	0.3718208554927388	0.0
3	0.0	0.0	0.0	0.5089678923548147	0.0
4	0.0	0.0	0.0	0.6918306081709157	0.0
5	0.0	0.0	0.0	1.223656340002743	0.0
6	0.0	0.0	0.0	1.7295765204272893	0.0
7	0.0	0.0	0.0	2.2248297090958964	0.0
8	0.0	0.0	0.0	3.2869573167944166	0.0
9	0.0	0.0	0.0	3.8614510156500006	0.0
10	0.0	0.0	0.0	4.9814851500236195	0.0
11	0.0	0.0	0.0	5.962848391570029	0.0
12	0.0	0.0	0.0	6.805540740289228	0.0
13	0.0	0.0	0.0	7.119455069106868	0.0
14	0.0	0.0	0.0	7.30993706474864	0.0
15	0.0	0.0	0.0	7.536991603553632	0.0
16	0.0	0.0	0.0	7.93776572238392	0.0
17	0.0	0.0	0.0	8.448257470703869	0.0
18	0.0	0.0	0.0	9.013608033768648	0.0
19	0.0	0.0	0.0	9.396095881017326	0.0
20	0.0	0.0	0.0	9.728296481416576	0.0
21	0.0	0.0	0.0	10.142785303933072	0.0
22	0.0	0.0	0.0	10.607561373298996	0.0
23	0.0	0.0	0.0	11.079956722490591	0.0
24	0.0	0.0	0.0	11.447206010087926	0.0
25	0.0	0.0	0.0	11.765691906800969	0.0
26	0.0	0.0	0.0	12.113131066851562	0.0
27	0.0	0.0	0.0	12.45599865900675	0.0
28	0.0	0.0	0.0	12.768389131859257	0.0
29	0.0	0.0	0.0	13.217926641573838	0.0
30	0.0	0.0	0.0	13.62327232829953	0.0
31	0.0	0.0	0.0	13.985950048001463	0.0
32	0.0	0.0	0.0	14.32424607226125	0.0
33	0.0	0.0	0.0	14.642731968974292	0.0
34	0.0	0.0	0.0	14.974932569373543	0.0
35	0.0	0.0	0.0	15.340658001005744	0.0
36	0.0	0.0	0.0	15.70333572070768	0.0
37	0.0	0.0	0.0	16.073632720235285	0.0
38	0.0	0.0	0.0	16.424119592216144	0.0
39	0.0	0.0	0.0	16.834036846837236	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGATT	40	6.6465873E-9	45.000004	6
TGACTCT	20	6.984699E-4	45.000004	5
GGATTCC	20	6.984699E-4	45.000004	8
AAGGATC	20	6.984699E-4	45.000004	6
TGGGATC	20	6.984699E-4	45.000004	6
AGGATTT	50	2.1827873E-11	45.0	7
AGGATTA	25	3.850591E-5	45.0	7
CTAAGGA	25	3.850591E-5	45.0	4
AAGGGCA	25	3.850591E-5	45.0	5
GCTAAGG	25	3.850591E-5	45.0	3
ACGGGAT	25	3.850591E-5	45.0	5
TTAGGAC	25	3.850591E-5	45.0	5
CGGTTTA	50	2.1827873E-11	45.0	1
CAGGATT	35	1.1886732E-7	45.0	6
CGTTTTA	615	0.0	44.999996	1
CGTTTTT	970	0.0	44.304127	1
CGTTATT	240	0.0	44.062504	1
CGGTTTT	125	0.0	43.2	1
GTTTTAT	675	0.0	40.666668	2
CTTAGGG	45	1.881017E-8	40.0	3
>>END_MODULE