SRR2936174.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936174.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90273
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2523	2.794855604665847	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1380	1.5286962879266226	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	632	0.700098589833062	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	608	0.6735125674343381	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	559	0.6192327717036101	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	555	0.6148017679704896	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	401	0.44420812424534467	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	357	0.3954670831810176	No Hit
GAATCTATCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	356	0.39435933224773745	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	321	0.3555880495829318	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	308	0.34118728745028964	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	150	0.16616263999202419	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	146	0.16173163625890355	No Hit
GAATCTATCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTT	137	0.1517618778593821	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	122	0.13514561386017968	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT	119	0.1318223610603392	No Hit
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	118	0.13071461012705904	TruSeq Adapter, Index 21 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	118	0.13071461012705904	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT	118	0.13071461012705904	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTT	107	0.11852934986097727	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTC	96	0.1063440895948955	No Hit
GAATGAATGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT	92	0.10191308586177483	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGCT	55	1.8189894E-12	45.000004	7
ATCTATC	40	6.6957E-9	45.0	3
GTTTATT	35	1.1953671E-7	45.0	3
CGGTTTA	35	1.1953671E-7	45.0	1
GACCGAT	25	3.8622078E-5	45.0	9
ACCGATT	25	3.8622078E-5	45.0	10
CGATTGG	20	6.9987506E-4	45.0	10
AGGCGAT	50	2.1827873E-11	45.0	7
CGTTATT	310	0.0	45.0	1
TTGAGGA	20	6.9987506E-4	45.0	4
AATCTAT	40	6.6957E-9	45.0	2
CGAATGG	20	6.9987506E-4	45.0	2
AAGGGCG	20	6.9987506E-4	45.0	5
TAAGGCG	20	6.9987506E-4	45.0	5
CAGTCTC	20	6.9987506E-4	45.0	6
CGTTTTA	490	0.0	45.0	1
CGAAGGA	20	6.9987506E-4	45.0	4
AAGGCGA	30	2.1432643E-6	44.999996	6
CGTTTTT	910	0.0	43.763737	1
ACGGCTG	115	0.0	43.043476	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content