##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936174.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90273
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.447453834479855	31.0	31.0	34.0	30.0	34.0
2	31.577226856313626	31.0	31.0	34.0	30.0	34.0
3	30.443809333909364	31.0	30.0	34.0	25.0	34.0
4	34.84236704219423	35.0	35.0	37.0	32.0	37.0
5	31.468567567268174	35.0	35.0	37.0	0.0	37.0
6	33.556334673711966	35.0	35.0	37.0	17.0	37.0
7	21.422861763761038	32.0	0.0	37.0	0.0	37.0
8	28.342527666079558	35.0	17.0	37.0	17.0	37.0
9	34.72112370254672	37.0	32.0	39.0	28.0	39.0
10	36.30643714067329	37.0	35.0	39.0	32.0	39.0
11	36.76888992278976	38.0	37.0	39.0	33.0	39.0
12	37.12138734726884	39.0	37.0	39.0	34.0	39.0
13	36.95809378219401	39.0	37.0	39.0	33.0	39.0
14	38.46358268806841	40.0	38.0	41.0	34.0	41.0
15	38.51774063119648	40.0	38.0	41.0	34.0	41.0
16	38.556467603823954	40.0	38.0	41.0	34.0	41.0
17	38.472987493491964	40.0	38.0	41.0	34.0	41.0
18	38.19205077930278	40.0	38.0	41.0	34.0	41.0
19	37.91013924429231	39.0	37.0	41.0	34.0	41.0
20	37.685398734948436	39.0	35.0	40.0	34.0	41.0
21	37.61110187985334	39.0	35.0	40.0	33.0	41.0
22	37.44640147109324	39.0	35.0	40.0	33.0	41.0
23	37.306104815393304	38.0	35.0	40.0	33.0	41.0
24	37.008828774938245	38.0	35.0	40.0	33.0	41.0
25	36.89125209087989	38.0	35.0	40.0	33.0	41.0
26	36.73708639349529	38.0	35.0	40.0	33.0	41.0
27	36.71886389064283	38.0	35.0	40.0	33.0	41.0
28	36.63830824277469	38.0	35.0	40.0	33.0	41.0
29	36.59811903891529	38.0	35.0	40.0	33.0	41.0
30	36.38586288258948	38.0	35.0	40.0	32.0	41.0
31	36.184407297863146	38.0	35.0	40.0	31.0	41.0
32	35.79977401880961	38.0	34.0	40.0	30.0	41.0
33	35.30522969215602	38.0	34.0	40.0	25.0	41.0
34	34.58921272141172	38.0	33.0	40.0	21.0	41.0
35	33.75604001196371	38.0	33.0	40.0	16.0	41.0
36	33.336435035946515	37.0	33.0	40.0	15.0	41.0
37	32.92029732035049	37.0	33.0	40.0	12.0	41.0
38	32.73153656131955	37.0	32.0	40.0	10.0	41.0
39	32.550640833914905	37.0	31.0	40.0	10.0	41.0
40	32.25780687470229	37.0	30.0	40.0	10.0	41.0
41	32.16293908477618	37.0	30.0	40.0	9.0	41.0
42	31.89533969182369	37.0	30.0	40.0	8.0	41.0
43	31.854087047068337	37.0	30.0	40.0	8.0	41.0
44	31.709680635405935	36.0	30.0	40.0	8.0	41.0
45	31.598916619587254	36.0	29.0	40.0	8.0	41.0
46	31.402966556999324	36.0	28.0	40.0	8.0	41.0
47	31.214239030496383	36.0	27.0	40.0	8.0	41.0
48	31.04296965870194	35.0	26.0	40.0	8.0	41.0
49	30.89126316838922	35.0	27.0	40.0	7.0	41.0
50	30.698669591129132	35.0	26.0	40.0	7.0	41.0
51	28.819503062931332	34.0	21.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	3.0
11	3.0
12	2.0
13	4.0
14	2.0
15	2.0
16	7.0
17	21.0
18	40.0
19	96.0
20	166.0
21	333.0
22	583.0
23	992.0
24	1677.0
25	2747.0
26	3261.0
27	3348.0
28	3003.0
29	2711.0
30	2666.0
31	2939.0
32	3587.0
33	4709.0
34	6237.0
35	8073.0
36	8993.0
37	13399.0
38	12761.0
39	7904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.92517142445693	15.894010390703755	19.763384400651358	29.417433784187963
2	42.671673700885094	25.60787832463749	19.535187708395643	12.185260266081773
3	23.431147740741974	25.101636148128453	40.17369534633833	11.293520764791245
4	20.13226546143365	20.144450721699734	46.015973768457904	13.707310048408717
5	19.393395588935782	31.151063994771416	35.408150831367074	14.047389584925726
6	18.56479789084222	29.852779900967068	40.68990728124688	10.892514926943827
7	35.62527001428999	40.64892049671552	21.01292745339138	2.712882035603115
8	60.60505355975763	8.046702779347092	27.789039912266126	3.5592037486291583
9	55.938098877848304	7.273492627917539	28.190045750113548	8.598362744120612
10	27.628416026940506	26.598207658989953	34.334740177018595	11.438636137050946
11	20.721588957938696	21.10043977712051	42.79020305074607	15.387768214194718
12	18.557043634309263	19.90960752384434	45.51527034661527	16.018078495231133
13	16.076789294694983	21.22450788164789	48.56047766220243	14.138225161454699
14	15.434293753392486	24.59760947348598	43.74286885336701	16.225227919754523
15	13.584349694814618	22.53386948478504	49.35584283229758	14.525937988102754
16	15.629257917649797	23.932958913517886	42.97519745660386	17.462585712228464
17	16.13660784509211	22.283517773863725	43.92010900269184	17.659765378352333
18	16.619587252002262	23.231752572751542	44.074086382417775	16.07457379282842
19	16.35705028081486	24.541114175888694	41.82645973879233	17.275375804504115
20	16.914249000254785	24.341719007898263	43.500271398978654	15.2437605928683
21	17.221096008773387	24.98643005106732	43.03058500326786	14.76188893689143
22	17.157954205576416	21.043944479523226	42.36593444329977	19.43216687160059
23	14.993408881946982	25.461655201444504	43.09261905553156	16.452316861076955
24	16.915356751188064	22.180496937068668	42.84559059741008	18.05855571433319
25	15.559469608853146	27.220763683493406	41.3423725809489	15.877394126704553
26	15.304686894198708	23.592879377000877	42.38033520543241	18.722098523368004
27	16.530967177339846	24.861254195606662	40.87822493990451	17.72955368714898
28	14.225737485183831	23.47213452527334	44.96361038184175	17.338517607701085
29	16.309416990683815	22.723294894375947	43.330785506186785	17.63650260875345
30	15.978199461633046	26.44976903393041	42.49221804969371	15.079813454742835
31	20.274057580893512	23.6194653993996	40.204712372470176	15.901764647236716
32	18.74646904390017	26.55278987072547	40.02747222314535	14.673268862229015
33	19.563989232660926	23.766796273525863	38.89202751653318	17.777186977280028
34	19.815448694515524	23.65380567833128	40.87047068337155	15.66027494378164
35	17.24546652930555	25.94131135555482	39.5334153069024	17.279806808237236
36	23.14977900368881	25.000830813199958	37.12184152515148	14.727548657959744
37	18.335493447653228	28.627607368759207	37.645807716593	15.391091466994562
38	21.08714676592115	26.835266358711905	34.449946274079736	17.627640601287204
39	21.55018665603226	26.833050856845347	35.28740597963954	16.32935650748286
40	20.597520853411318	25.72529992356519	37.58931241899571	16.08786680402778
41	20.780299757402545	27.304952754422697	34.452161775946294	17.462585712228464
42	18.434083280715164	26.31573117100351	38.18528242110044	17.064903127180887
43	21.892481694415828	25.790657228628717	33.58590054612121	18.73096053083425
44	19.865297486513136	25.88149280515769	34.95064969592237	19.30256001240681
45	18.31223067805435	25.603447320904365	34.624970921538	21.459351079503282
46	21.532462641099777	27.226302438159806	33.25579076800372	17.985444152736697
47	18.33217019485339	25.54805977424036	38.28165675229581	17.838113278610436
48	18.868321646560986	26.820865596579264	34.43111450821397	19.879698248645774
49	18.297829915921703	24.193280382838722	38.15315764403531	19.355732057204257
50	18.769731813499053	25.013016073466044	36.435035946517786	19.78221616651712
51	18.94254095909076	24.286331461234255	34.44440751941333	22.32672006026165
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	388.0
1	416.5
2	445.0
3	3673.0
4	6901.0
5	4765.0
6	2629.0
7	2214.0
8	1799.0
9	1719.0
10	1639.0
11	1591.5
12	1544.0
13	1489.0
14	1434.0
15	1331.5
16	1229.0
17	1173.5
18	1118.0
19	1071.5
20	1025.0
21	998.0
22	971.0
23	966.0
24	961.0
25	1015.5
26	1123.5
27	1177.0
28	1329.5
29	1482.0
30	1625.5
31	1769.0
32	1899.5
33	2030.0
34	2302.0
35	2574.0
36	2766.0
37	2958.0
38	3170.0
39	3382.0
40	3658.5
41	3935.0
42	4323.5
43	4712.0
44	5572.5
45	6433.0
46	7975.5
47	9518.0
48	7885.5
49	6253.0
50	5720.0
51	5187.0
52	4384.5
53	3582.0
54	3211.0
55	2840.0
56	2551.0
57	2262.0
58	2085.0
59	1908.0
60	1677.5
61	1447.0
62	1348.5
63	1250.0
64	1080.5
65	911.0
66	745.5
67	580.0
68	469.0
69	358.0
70	300.0
71	242.0
72	189.0
73	136.0
74	112.0
75	79.0
76	70.0
77	43.5
78	17.0
79	12.5
80	8.0
81	6.5
82	5.0
83	4.0
84	3.0
85	2.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	90273.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.74250329555903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.3834374197964	53.337099686506484
2	9.509795534262983	12.313759374342274
3	3.909658653434853	7.593632647635505
4	1.8513132004448627	4.7943460392365385
5	0.941055693386945	3.0463150665204433
6	0.5269911882966892	2.047123724701738
7	0.2994268115322098	1.3569948932681974
8	0.1574129523483617	0.8153046868941987
9	0.087261527932244	0.5084576783755941
>10	0.27889468731285827	3.1227498809167744
>50	0.02053212421935153	0.9449115460879777
>100	0.023954144922576782	3.1881071859803045
>500	0.006844041406450508	2.6076456969415
>1k	0.003422020703225254	4.323551892592469
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2523	2.794855604665847	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1380	1.5286962879266226	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	632	0.700098589833062	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	608	0.6735125674343381	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	559	0.6192327717036101	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	555	0.6148017679704896	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	401	0.44420812424534467	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	357	0.3954670831810176	No Hit
GAATCTATCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	356	0.39435933224773745	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	321	0.3555880495829318	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	308	0.34118728745028964	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	150	0.16616263999202419	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	146	0.16173163625890355	No Hit
GAATCTATCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTT	137	0.1517618778593821	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	122	0.13514561386017968	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT	119	0.1318223610603392	No Hit
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	118	0.13071461012705904	TruSeq Adapter, Index 21 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	118	0.13071461012705904	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT	118	0.13071461012705904	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTT	107	0.11852934986097727	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTC	96	0.1063440895948955	No Hit
GAATGAATGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT	92	0.10191308586177483	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08197356906273194	0.0
2	0.0	0.0	0.0	0.4696863957107884	0.0
3	0.0	0.0	0.0	0.6292025301031317	0.0
4	0.0	0.0	0.0	0.933834036755176	0.0
5	0.0	0.0	0.0	2.1523600633633535	0.0
6	0.0	0.0	0.0	2.616507704407741	0.0
7	0.0	0.0	0.0	3.1415816467825373	0.0
8	0.0	0.0	0.0	4.007842876607624	0.0
9	0.0	0.0	0.0	4.426572729387525	0.0
10	0.0	0.0	0.0	6.438248424224297	0.0
11	0.0	0.0	0.0	7.115084244458476	0.0
12	0.0	0.0	0.0	8.906317503572497	0.0
13	0.0	0.0	0.0	9.290707077420713	0.0
14	0.0	0.0	0.0	9.48456349074474	0.0
15	0.0	0.0	0.0	9.772578733397584	0.0
16	0.0	0.0	0.0	10.186877582444364	0.0
17	0.0	0.0	0.0	10.641055465089229	0.0
18	0.0	0.0	0.0	11.155051898131225	0.0
19	0.0	0.0	0.0	11.449713646383747	0.0
20	0.0	0.0	0.0	11.725543628770508	0.0
21	0.0	0.0	0.0	12.060084410621116	0.0
22	0.0	0.0	0.0	12.44004298073621	0.0
23	0.0	0.0	0.0	12.806708539651945	0.0
24	0.0	0.0	0.0	13.094723782304786	0.0
25	0.0	0.0	0.0	13.348398746025943	0.0
26	0.0	0.0	0.0	13.59764270601398	0.0
27	0.0	0.0	0.0	13.853533171601697	0.0
28	0.0	0.0	0.0	14.129363153988457	0.0
29	0.0	0.0	0.0	14.386361370509455	0.0
30	0.0	0.0	0.0	14.69542388089462	0.0
31	0.0	0.0	0.0	14.997839885680104	0.0
32	0.0	0.0	0.0	15.30801014699855	0.0
33	0.0	0.0	0.0	15.576085872852348	0.0
34	0.0	0.0	0.0	15.844161598706147	0.0
35	0.0	0.0	0.0	16.187564388023	0.0
36	0.0	0.0	0.0	16.4777951325424	0.0
37	0.0	0.0	0.0	16.787965393860844	0.0
38	0.0	0.0	0.0	17.066010878114163	0.0
39	0.0	0.0	0.0	17.357349373566848	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGCT	55	1.8189894E-12	45.000004	7
ATCTATC	40	6.6957E-9	45.0	3
GTTTATT	35	1.1953671E-7	45.0	3
CGGTTTA	35	1.1953671E-7	45.0	1
GACCGAT	25	3.8622078E-5	45.0	9
ACCGATT	25	3.8622078E-5	45.0	10
CGATTGG	20	6.9987506E-4	45.0	10
AGGCGAT	50	2.1827873E-11	45.0	7
CGTTATT	310	0.0	45.0	1
TTGAGGA	20	6.9987506E-4	45.0	4
AATCTAT	40	6.6957E-9	45.0	2
CGAATGG	20	6.9987506E-4	45.0	2
AAGGGCG	20	6.9987506E-4	45.0	5
TAAGGCG	20	6.9987506E-4	45.0	5
CAGTCTC	20	6.9987506E-4	45.0	6
CGTTTTA	490	0.0	45.0	1
CGAAGGA	20	6.9987506E-4	45.0	4
AAGGCGA	30	2.1432643E-6	44.999996	6
CGTTTTT	910	0.0	43.763737	1
ACGGCTG	115	0.0	43.043476	8
>>END_MODULE