##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936172.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	103385
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4694878367268	31.0	31.0	34.0	30.0	34.0
2	31.63053634473086	31.0	31.0	34.0	30.0	34.0
3	30.55522561299995	31.0	30.0	34.0	26.0	34.0
4	34.903090390288725	37.0	35.0	37.0	32.0	37.0
5	31.548445132272573	35.0	35.0	37.0	0.0	37.0
6	33.60845383759733	36.0	35.0	37.0	17.0	37.0
7	21.48379358707743	32.0	0.0	37.0	0.0	37.0
8	28.44715384243362	35.0	17.0	37.0	17.0	37.0
9	34.78996953136335	37.0	32.0	39.0	30.0	39.0
10	36.296029404652515	37.0	35.0	39.0	32.0	39.0
11	36.729370798471734	39.0	37.0	39.0	33.0	39.0
12	37.03267398558785	39.0	37.0	39.0	34.0	39.0
13	36.78197997775306	39.0	37.0	39.0	33.0	39.0
14	38.09494607534942	40.0	38.0	41.0	33.0	41.0
15	38.23150360303719	40.0	38.0	41.0	33.0	41.0
16	38.326739855878515	40.0	38.0	41.0	33.0	41.0
17	38.2664022827296	40.0	37.0	41.0	33.0	41.0
18	38.04587706146926	39.0	37.0	41.0	33.0	41.0
19	37.80807660685786	39.0	37.0	41.0	33.0	41.0
20	37.54948977124341	39.0	35.0	41.0	33.0	41.0
21	37.398587802872754	39.0	35.0	40.0	33.0	41.0
22	37.29671615805001	39.0	35.0	40.0	33.0	41.0
23	37.15941384146636	38.0	35.0	40.0	33.0	41.0
24	36.93557092421531	38.0	35.0	40.0	33.0	41.0
25	36.746104367171256	38.0	35.0	40.0	32.0	41.0
26	36.62598055810804	38.0	35.0	40.0	32.0	41.0
27	36.55457754993471	38.0	35.0	40.0	32.0	41.0
28	36.4363785849011	38.0	35.0	40.0	32.0	41.0
29	36.374793248537024	38.0	35.0	40.0	32.0	41.0
30	36.128006964259804	38.0	35.0	40.0	31.0	41.0
31	35.9104512259999	38.0	34.0	40.0	30.0	41.0
32	35.618184456159014	38.0	34.0	40.0	29.0	41.0
33	35.148812690428976	38.0	34.0	40.0	25.0	41.0
34	34.486366494172266	37.0	33.0	40.0	22.0	41.0
35	33.864767616191905	37.0	33.0	40.0	18.0	41.0
36	33.42487788363883	37.0	33.0	40.0	15.0	41.0
37	33.08229433670262	37.0	32.0	40.0	15.0	41.0
38	32.86012477632152	37.0	32.0	40.0	11.0	41.0
39	32.70943560477826	37.0	32.0	40.0	10.0	41.0
40	32.555428737244284	37.0	31.0	40.0	10.0	41.0
41	32.391933065725205	36.0	31.0	40.0	10.0	41.0
42	32.19469942448131	36.0	31.0	40.0	10.0	41.0
43	32.098853798907	36.0	30.0	40.0	10.0	41.0
44	31.96282826328771	36.0	30.0	40.0	10.0	41.0
45	31.883783914494366	35.0	30.0	40.0	10.0	41.0
46	31.752575325240606	35.0	30.0	40.0	10.0	41.0
47	31.640847318276347	35.0	29.0	40.0	9.0	41.0
48	31.529825409875706	35.0	29.0	40.0	8.0	41.0
49	31.397359384823716	35.0	28.0	40.0	8.0	41.0
50	31.217971659331624	35.0	28.0	40.0	8.0	41.0
51	29.6037432896455	34.0	24.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	3.0
11	5.0
12	7.0
13	2.0
14	1.0
15	3.0
16	9.0
17	33.0
18	52.0
19	125.0
20	200.0
21	364.0
22	678.0
23	1106.0
24	1861.0
25	2698.0
26	3397.0
27	3622.0
28	3363.0
29	3204.0
30	3171.0
31	3563.0
32	4340.0
33	6050.0
34	7577.0
35	9518.0
36	10136.0
37	15000.0
38	14372.0
39	8915.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.44198868307782	17.12240653866615	20.61904531605165	28.81655946220438
2	44.65057793683803	21.240992407022297	20.65967016491754	13.448759491222132
3	27.01939352904193	20.663539198142864	39.53184697973594	12.785220293079266
4	23.286743724911737	20.694491463945447	41.29515887217681	14.723605938966
5	18.975673453595782	31.77636987957634	35.10664022827296	14.141316438554915
6	20.417855588334866	28.534120036755816	39.99322919185568	11.054795183053635
7	38.11868259418677	40.61324176621366	18.61682062194709	2.6512550176524643
8	64.00928567974078	5.774532088794312	26.445809353387823	3.7703728780770907
9	58.49301155873676	6.978768680176041	28.25071335300092	6.277506408086279
10	33.717657300382065	20.525221260337574	34.31929196691977	11.437829472360594
11	26.16723896116458	20.21666586061808	38.99985491125405	14.616240266963294
12	23.842917251051894	18.502684141800067	42.20728345504667	15.447115152101368
13	20.245683609807998	19.916815785655558	43.92319969047735	15.914300914059098
14	17.28877496735503	21.68496396962809	44.396189002273054	16.63007206074382
15	16.629104802437492	20.448807854137446	47.29409488803985	15.627992455385211
16	20.74285437926198	20.53489384340088	43.00623881607583	15.716012961261306
17	20.032886782415243	20.939207815447112	41.598877980364655	17.429027421772986
18	21.312569521690765	20.570682400735116	42.50713353000919	15.609614547564926
19	19.754316390192	22.557430961938387	40.791217294578516	16.897035353291095
20	20.159597620544567	23.63592397349712	41.6694878367268	14.534990569231512
21	19.42641582434589	23.22193741838758	42.82826328771098	14.523383469555545
22	18.897325530782997	19.90520868597959	43.17937805290903	18.018087730328382
23	18.283116506263	22.18020022246941	43.10103013009624	16.43565314117135
24	18.653576437587656	21.12588866856894	42.91725105189341	17.303283841949995
25	18.47946994244813	22.683174541761378	41.207138366300725	17.63021714948977
26	17.64569328239106	21.227450790733666	41.56308942303042	19.56376650384485
27	17.183343811964985	21.459592784253033	43.17647627799004	18.18058712579194
28	15.780819267785462	22.59225226096629	42.676403733617065	18.950524737631184
29	18.570392223243218	22.487788363882576	40.99143976398897	17.950379648885235
30	18.486240750592444	21.84843062339798	42.88726604439715	16.778062581612417
31	20.052231948541856	21.85133239831697	40.97112733955603	17.125308313585144
32	20.457513178894423	22.4162112492141	40.0599700149925	17.06630555689897
33	19.6691976592349	22.601924844029597	39.82492624655414	17.90395125018136
34	18.336315713111187	23.76650384485177	40.63548870725928	17.261691734777774
35	18.322774096822556	25.607196401799097	39.133336557527684	16.936692943850655
36	20.481694636552692	26.747593944963004	36.02843739420612	16.742274024278185
37	18.959230062388162	27.335686995212072	36.2073801808773	17.497702761522465
38	19.782366881075593	27.607486579291002	34.51661266141123	18.09353387822218
39	20.341442182134738	26.442907578468834	34.31832470861343	18.897325530782997
40	21.926778546210766	24.81211007399526	35.48193645112928	17.779174928664702
41	19.840402379455433	25.08874594960584	34.5659428350341	20.50490883590463
42	20.649997581854233	24.39715626057939	35.62412342215989	19.32872273540649
43	20.65580113169222	24.173719591816994	35.55738259902307	19.613096677467716
44	19.68467379213619	24.71731875997485	35.47710015959762	20.12090728829134
45	19.632441843594332	25.046186584127287	34.53982686076317	20.78154471151521
46	20.17604101175219	25.558833486482563	34.488562170527636	19.776563331237607
47	18.695168544759877	25.332495042801177	36.92218406925569	19.050152343183246
48	19.47961503119408	25.697151424287856	35.539004691202784	19.284228853315277
49	19.66726314262224	24.86530928084345	36.00812496977318	19.459302606761135
50	18.331479421579534	24.874014605600426	35.94235140494269	20.852154567877353
51	18.59940997243314	23.96092276442424	35.26527059051119	22.174396672631428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	152.0
1	242.0
2	332.0
3	4546.0
4	8760.0
5	5713.0
6	2666.0
7	2232.0
8	1798.0
9	1690.5
10	1583.0
11	1534.0
12	1485.0
13	1401.5
14	1318.0
15	1222.0
16	1126.0
17	1062.5
18	999.0
19	958.5
20	918.0
21	903.0
22	888.0
23	947.5
24	1007.0
25	1110.0
26	1301.5
27	1390.0
28	1530.0
29	1670.0
30	1826.5
31	1983.0
32	2183.0
33	2383.0
34	2564.5
35	2746.0
36	2883.5
37	3021.0
38	3253.0
39	3485.0
40	3681.5
41	3878.0
42	4103.5
43	4329.0
44	4932.0
45	5535.0
46	6376.5
47	7218.0
48	6955.0
49	6692.0
50	6554.5
51	6417.0
52	5867.5
53	5318.0
54	5030.5
55	4743.0
56	4484.0
57	4225.0
58	4041.5
59	3858.0
60	3466.5
61	3075.0
62	2712.5
63	2350.0
64	2069.0
65	1788.0
66	1434.5
67	1081.0
68	926.5
69	772.0
70	634.5
71	497.0
72	391.0
73	285.0
74	224.5
75	128.5
76	93.0
77	83.5
78	74.0
79	51.0
80	28.0
81	26.0
82	24.0
83	15.0
84	6.0
85	5.0
86	4.0
87	4.0
88	4.0
89	3.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	103385.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.81322242104754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0540703399421	53.344295594138416
2	10.148292941020856	13.357837210427045
3	4.197469172998633	8.287469168641486
4	1.991446333828133	5.242540020312425
5	1.061124909980747	3.4918024858538477
6	0.5878808365544305	2.3214199351936937
7	0.3233344601049367	1.4895777917492867
8	0.1719551446921709	0.9053537747255404
9	0.12198527358504431	0.722541954829037
>10	0.3218647580135507	3.4618174783575957
>50	0.0044091062741582285	0.19441891957247184
>100	0.011757616731088608	1.4876432751366253
>500	0.001469702091386076	0.7380180877303284
>1k	0.002939404182772152	4.955264303332204
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3291	3.183247086134352	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1832	1.7720172171978528	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	763	0.7380180877303284	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	356	0.34434395705373116	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	241	0.23310925182570005	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	205	0.19828795279779465	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC	182	0.17604101175218842	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	175	0.16927020360787348	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCG	154	0.14895777917492867	No Hit
GAATCTATCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	117	0.11316922184069254	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGC	108	0.1044638970837162	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0512646902355274	0.0
2	0.0	0.0	0.0	0.24278183488900712	0.0
3	0.0	0.0	0.0	0.31435894955747934	0.0
4	0.0	0.0	0.0	0.4284954297045026	0.0
5	0.0	0.0	0.0	0.910190066257194	0.0
6	0.0	0.0	0.0	1.2332543405716496	0.0
7	0.0	0.0	0.0	1.5621221647240895	0.0
8	0.0	0.0	0.0	2.2846641195531268	0.0
9	0.0	0.0	0.0	2.67350195869807	0.0
10	0.0	0.0	0.0	3.7249117376795473	0.0
11	0.0	0.0	0.0	4.494849349518789	0.0
12	0.0	0.0	0.0	5.405039415775983	0.0
13	0.0	0.0	0.0	5.709725782270155	0.0
14	0.0	0.0	0.0	5.883832277409682	0.0
15	0.0	0.0	0.0	6.1266141122986895	0.0
16	0.0	0.0	0.0	6.459350969676452	0.0
17	0.0	0.0	0.0	6.835614450839096	0.0
18	0.0	0.0	0.0	7.259273589011945	0.0
19	0.0	0.0	0.0	7.548483822604827	0.0
20	0.0	0.0	0.0	7.871548096919282	0.0
21	0.0	0.0	0.0	8.229433670261644	0.0
22	0.0	0.0	0.0	8.61053344295594	0.0
23	0.0	0.0	0.0	8.975189824442618	0.0
24	0.0	0.0	0.0	9.243120375296224	0.0
25	0.0	0.0	0.0	9.495574793248537	0.0
26	0.0	0.0	0.0	9.76157082748948	0.0
27	0.0	0.0	0.0	10.07496251874063	0.0
28	0.0	0.0	0.0	10.343860327900567	0.0
29	0.0	0.0	0.0	10.619528945204816	0.0
30	0.0	0.0	0.0	10.98031629346617	0.0
31	0.0	0.0	0.0	11.231803453112153	0.0
32	0.0	0.0	0.0	11.52778449484935	0.0
33	0.0	0.0	0.0	11.838274411181507	0.0
34	0.0	0.0	0.0	12.151666102432655	0.0
35	0.0	0.0	0.0	12.465057793683803	0.0
36	0.0	0.0	0.0	12.751366252357693	0.0
37	0.0	0.0	0.0	13.07636504328481	0.0
38	0.0	0.0	0.0	13.465202882429752	0.0
39	0.0	0.0	0.0	14.054263190985152	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	30	2.146313E-6	45.000004	13
ACGCCGG	30	2.146313E-6	45.000004	27
CAACGAG	35	1.1976408E-7	45.000004	14
CACGCCG	30	2.146313E-6	45.000004	26
GTAGGAT	35	1.1976408E-7	45.000004	33
TGAAACG	30	2.146313E-6	45.000004	4
AACGAGC	30	2.146313E-6	45.000004	15
CGGTAGT	30	2.146313E-6	45.000004	12
ATGGGAT	20	7.003494E-4	45.0	5
CGTATGG	20	7.003494E-4	45.0	2
CGGTTTA	25	3.8661325E-5	45.0	1
TATGGGT	20	7.003494E-4	45.0	4
TATGGGA	25	3.8661325E-5	45.0	4
AGGGTTC	20	7.003494E-4	45.0	7
CAATTAA	20	7.003494E-4	45.0	32
CTACGGG	20	7.003494E-4	45.0	3
CTACGAA	25	3.8661325E-5	45.0	11
GGAACAC	40	6.712071E-9	45.0	8
CGAATAT	25	3.8661325E-5	45.0	14
GCGATGA	20	7.003494E-4	45.0	9
>>END_MODULE