##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936170.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	116836
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.309895922489645	31.0	31.0	34.0	28.0	34.0
2	31.467501455030984	31.0	31.0	34.0	30.0	34.0
3	30.571056866034443	31.0	30.0	34.0	26.0	34.0
4	34.844945051182854	35.0	35.0	37.0	32.0	37.0
5	31.438794549625115	35.0	35.0	37.0	0.0	37.0
6	33.49365777671266	35.0	35.0	37.0	17.0	37.0
7	21.48818001300969	32.0	0.0	37.0	0.0	37.0
8	28.40485809168407	35.0	17.0	37.0	17.0	37.0
9	34.70860864801945	37.0	32.0	39.0	28.0	39.0
10	35.95256598993461	37.0	35.0	39.0	32.0	39.0
11	36.4380156800986	37.0	35.0	39.0	32.0	39.0
12	36.94297134444863	39.0	37.0	39.0	33.0	39.0
13	36.80103735150125	39.0	37.0	39.0	33.0	39.0
14	38.223492759081104	40.0	37.0	41.0	34.0	41.0
15	38.312164059022905	40.0	38.0	41.0	34.0	41.0
16	38.330951076722926	40.0	38.0	41.0	34.0	41.0
17	38.26982265740012	40.0	37.0	41.0	34.0	41.0
18	38.05143962477319	39.0	37.0	40.0	33.0	41.0
19	37.83835461672772	39.0	37.0	40.0	33.0	41.0
20	37.6431921667979	39.0	35.0	40.0	33.0	41.0
21	37.53706905405868	39.0	35.0	40.0	33.0	41.0
22	37.42960217741107	39.0	35.0	40.0	33.0	41.0
23	37.29733130199596	39.0	35.0	40.0	33.0	41.0
24	37.04565373686193	38.0	35.0	40.0	33.0	41.0
25	36.92352528330309	38.0	35.0	40.0	32.0	41.0
26	36.77637029682632	38.0	35.0	40.0	32.0	41.0
27	36.71970111951796	38.0	35.0	40.0	32.0	41.0
28	36.653574240816184	38.0	35.0	40.0	32.0	41.0
29	36.61410010613167	38.0	35.0	40.0	32.0	41.0
30	36.361617994453766	38.0	35.0	40.0	31.0	41.0
31	36.18496867403882	38.0	35.0	40.0	30.0	41.0
32	35.916001917217294	38.0	35.0	40.0	30.0	41.0
33	35.39372282515663	38.0	34.0	40.0	25.0	41.0
34	34.90941148276216	38.0	33.0	40.0	24.0	41.0
35	34.23716149132117	38.0	33.0	40.0	20.0	41.0
36	33.62936081344791	38.0	33.0	40.0	15.0	41.0
37	33.34068266630148	38.0	33.0	40.0	15.0	41.0
38	33.12923242836112	38.0	32.0	40.0	12.0	41.0
39	33.00540929165668	38.0	32.0	40.0	10.0	41.0
40	32.9263069601835	38.0	32.0	40.0	10.0	41.0
41	32.67571638878428	38.0	31.0	40.0	10.0	41.0
42	32.4582149337533	37.0	31.0	40.0	10.0	41.0
43	32.24119278304632	37.0	30.0	40.0	10.0	41.0
44	32.0873360950392	37.0	30.0	40.0	9.0	41.0
45	32.03151425930364	37.0	30.0	40.0	9.0	41.0
46	31.77472696771543	37.0	29.0	40.0	8.0	41.0
47	31.614630764490396	37.0	28.0	40.0	8.0	41.0
48	31.39057311102742	36.0	27.0	40.0	8.0	41.0
49	31.19885994042932	36.0	27.0	40.0	8.0	41.0
50	30.976514088123523	36.0	26.0	40.0	8.0	41.0
51	28.950657331644358	34.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	4.0
11	3.0
12	1.0
13	1.0
14	1.0
15	5.0
16	10.0
17	21.0
18	37.0
19	123.0
20	210.0
21	417.0
22	749.0
23	1218.0
24	2177.0
25	3292.0
26	4002.0
27	3921.0
28	3657.0
29	3427.0
30	3472.0
31	3973.0
32	4835.0
33	6334.0
34	7952.0
35	10198.0
36	12081.0
37	17926.0
38	16561.0
39	10224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.991235578075255	18.751925776301825	22.107911944948473	25.14892670067445
2	39.671847718169055	24.452223629703173	21.9761032558458	13.899825396281967
3	24.82197267965353	23.57749323838543	38.435071382108255	13.165462699852784
4	21.81262624533534	22.40918894861173	41.676366873224005	14.101817932828922
5	18.526823924132973	32.80838097846554	34.41918586736964	14.245609230031839
6	19.03094936492177	30.866342565647575	38.77144030949365	11.331267759937006
7	39.1497483652299	40.47896196377829	17.328563114108665	3.0427265568831525
8	65.54486630832962	6.513403403060701	23.465370262590298	4.476360026019377
9	61.367215584237734	7.1373549248519295	24.633674552363996	6.861754938546339
10	28.189941456400426	25.49556643500291	33.21493375329522	13.099558355301449
11	19.382724502721764	23.797459687082746	39.853298640829884	16.966517169365606
12	18.296586668492587	20.366154267520283	44.685713307542194	16.651545756444932
13	16.981067479201617	23.204320586120716	45.28398781197576	14.530624122701907
14	15.761409154712588	26.16145708514499	41.03272963812524	17.044404122017188
15	14.517785614023074	25.26276147762676	45.79838405970762	14.421068848642541
16	15.654421582388988	24.855352802218494	41.80132835769797	17.688897257694546
17	16.07210106474032	24.94265466123455	40.65870108528193	18.326543188743198
18	16.690917183060016	24.66791057550755	42.16166250128385	16.479509740148586
19	15.537163203122326	26.888972576945463	39.888390564552026	17.68547365538019
20	16.32630353658119	26.396829744256905	41.69177308363861	15.585093635523299
21	16.123455099455647	26.512376322366393	41.85781779588483	15.50635078229313
22	15.46355575336369	23.467082063747473	40.44044643774179	20.628915745147044
23	14.353452702934028	25.41682358177274	41.485501044198706	18.744222671094526
24	16.465815330891164	23.714437330959637	41.212468759628884	18.60727857852032
25	14.32349618268342	28.09408059159848	39.438186860214316	18.144236365503783
26	15.431887431955904	25.571741586497314	40.84357561025711	18.15279537128967
27	16.026738334075112	26.840186243965903	39.77712348933548	17.355951932623505
28	14.029066383648875	25.65818754493478	42.010168098873635	18.30257797254271
29	17.39532335923859	23.685336711287615	39.80194460611455	19.117395323359236
30	15.804204183642028	27.393098017734257	40.048443972748125	16.754253825875587
31	17.392755657502825	27.330617275497275	37.16833852579685	18.108288541203056
32	17.546817761648807	26.70324215139169	37.39771988085864	18.35222020610086
33	15.797356979013319	25.323530418706564	39.50580300592283	19.373309596357288
34	18.296586668492587	25.118970180423844	37.6989968845219	18.885446266561676
35	18.080043822109623	25.532370159882227	36.91328015337738	19.474305864630765
36	18.758772980930534	27.33404087781163	37.259063987127256	16.648122154130576
37	17.09832585846828	27.764558868841792	37.39857578143722	17.7385394912527
38	16.514601663870724	28.162552637885586	35.83484542435551	19.488000273888183
39	19.340785374370913	25.329521722756688	36.39203670101681	18.937656201855592
40	20.92762504707453	24.34266835564381	36.233695093977886	18.496011503303777
41	19.347632578999622	26.290698072511898	34.709336163511246	19.652333184977234
42	19.250059913040502	25.886712999417988	36.090759697353555	18.772467390187956
43	18.591872368105722	27.055873189770274	34.91389640179397	19.438358040330037
44	17.689753158273135	26.49525831079462	34.20949022561539	21.605498305316853
45	18.490876099832242	25.25420247184087	33.68653497209764	22.568386456229245
46	20.48255674620836	26.383991235578076	33.535040569687425	19.59841144852614
47	17.72826868430963	25.59485090211921	37.10928138587421	19.567599027696943
48	18.21014071005512	25.20028073538978	35.382929918860626	21.206648635694478
49	18.021842582765586	23.6639391968229	37.43965900920949	20.874559211202026
50	17.60673080215002	23.487623677633607	36.26536341538567	22.640282104830703
51	18.03896059433736	23.432846040603923	34.23602314355165	24.29217022150707
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	577.0
1	506.5
2	436.0
3	3516.0
4	6596.0
5	4583.0
6	2570.0
7	2214.0
8	1858.0
9	1816.5
10	1775.0
11	1728.0
12	1681.0
13	1599.5
14	1518.0
15	1420.0
16	1322.0
17	1284.0
18	1246.0
19	1182.0
20	1118.0
21	1074.0
22	1030.0
23	1022.5
24	1015.0
25	1170.0
26	1442.5
27	1560.0
28	1762.0
29	1964.0
30	2261.0
31	2558.0
32	2825.5
33	3093.0
34	3509.0
35	3925.0
36	4393.0
37	4861.0
38	5045.5
39	5230.0
40	5741.5
41	6253.0
42	6944.0
43	7635.0
44	8713.0
45	9791.0
46	11172.5
47	12554.0
48	11482.0
49	10410.0
50	9539.5
51	8669.0
52	7003.0
53	5337.0
54	4281.5
55	3226.0
56	2657.0
57	2088.0
58	1804.0
59	1520.0
60	1232.0
61	944.0
62	740.0
63	536.0
64	409.0
65	282.0
66	204.0
67	126.0
68	114.0
69	102.0
70	74.0
71	46.0
72	37.5
73	29.0
74	18.5
75	9.5
76	11.0
77	6.5
78	2.0
79	1.5
80	1.0
81	2.0
82	3.0
83	3.0
84	3.0
85	1.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	116836.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.845218939368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.48207270376449	58.16186791742272
2	8.411656472226365	11.582046629463521
3	3.1826545327962608	6.573316443561916
4	1.5503008603113033	4.2692320860008905
5	0.9150131781789248	3.1497141292067514
6	0.491073648615048	2.028484371255435
7	0.32448157541399375	1.5637303570817214
8	0.1578895022129395	0.8695949878462117
9	0.11561987169923915	0.7163887842788182
>10	0.3282112486946143	3.5964942312301003
>50	0.01864836640310309	0.88500119826081
>100	0.01864836640310309	2.9117737683590676
>500	0.0012432244268735391	0.4536273066520593
>1k	0.0024864488537470783	3.238727789379986
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2434	2.083262008285118	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1350	1.155465781094868	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	530	0.4536273066520593	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	473	0.40484097367249827	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCG	428	0.3663254476360026	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTC	379	0.3243863192851518	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	296	0.25334657126228216	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCG	287	0.24564346605498305	No Hit
GAATCTATCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTC	272	0.23280495737615117	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGC	238	0.20370433770413227	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGC	181	0.1549180047245712	No Hit
GCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	156	0.1335204902598514	No Hit
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	131	0.11212297579513164	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	130	0.11126707521654286	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1549180047245712	0.0
2	0.0	0.0	0.0	0.7275154918004725	0.0
3	0.0	0.0	0.0	0.9483378410763805	0.0
4	0.0	0.0	0.0	1.27443596151871	0.0
5	0.0	0.0	0.0	2.357150193433531	0.0
6	0.0	0.0	0.0	2.9562805984456846	0.0
7	0.0	0.0	0.0	3.703481803553699	0.0
8	0.0	0.0	0.0	5.093464343181896	0.0
9	0.0	0.0	0.0	5.60700469033517	0.0
10	0.0	0.0	0.0	7.546475401417371	0.0
11	0.0	0.0	0.0	9.066554828991064	0.0
12	0.0	0.0	0.0	10.602040466979355	0.0
13	0.0	0.0	0.0	11.0950392002465	0.0
14	0.0	0.0	0.0	11.318429251258173	0.0
15	0.0	0.0	0.0	11.737820534766682	0.0
16	0.0	0.0	0.0	12.583450306412407	0.0
17	0.0	0.0	0.0	13.638775719812386	0.0
18	0.0	0.0	0.0	14.742887466191927	0.0
19	0.0	0.0	0.0	15.451573145263445	0.0
20	0.0	0.0	0.0	16.07038926358314	0.0
21	0.0	0.0	0.0	16.84412338662741	0.0
22	0.0	0.0	0.0	17.70087986579479	0.0
23	0.0	0.0	0.0	18.644938203978224	0.0
24	0.0	0.0	0.0	19.232941901468724	0.0
25	0.0	0.0	0.0	19.826936903009347	0.0
26	0.0	0.0	0.0	20.394398986613716	0.0
27	0.004279502892943956	0.0	0.0	20.949022561539252	0.0
28	0.005135403471532747	0.0	0.0	21.493375329521722	0.0
29	0.005135403471532747	0.0	0.0	22.065116916019036	0.0
30	0.005135403471532747	0.0	0.0	22.689068437810263	0.0
31	0.005135403471532747	0.0	0.0	23.268513129514876	0.0
32	0.005135403471532747	0.0	0.0	23.893320551884692	0.0
33	0.005135403471532747	0.0	0.0	24.464206237803417	0.0
34	0.005135403471532747	0.0	0.0	25.017118011571775	0.0
35	0.005135403471532747	0.0	0.0	25.590571399226267	0.0
36	0.005135403471532747	0.0	0.0	26.136635968365916	0.0
37	0.005135403471532747	0.0	0.0	26.67842103461262	0.0
38	0.005135403471532747	0.0	0.0	27.25786572631723	0.0
39	0.005135403471532747	0.0	0.0	27.834742716286076	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTGGGA	35	1.1994416E-7	45.000004	4
ACGTCTC	30	2.148734E-6	45.000004	34
ATAACGT	30	2.148734E-6	45.000004	31
TGATGGG	30	2.148734E-6	45.000004	3
AATAACG	30	2.148734E-6	45.000004	30
TTTAGGT	20	7.007261E-4	45.0	4
ATGGGCG	20	7.007261E-4	45.0	5
GGTGTGA	20	7.007261E-4	45.0	8
GGCACTA	25	3.869248E-5	45.0	8
TACGGCT	25	3.869248E-5	45.0	7
TGAAACG	20	7.007261E-4	45.0	4
CGTTATT	195	0.0	45.0	1
AAACGGC	20	7.007261E-4	45.0	6
AACGGCT	20	7.007261E-4	45.0	7
AGGCACC	25	3.869248E-5	45.0	7
GAAACGG	20	7.007261E-4	45.0	5
TAGGCAC	20	7.007261E-4	45.0	6
TCGAAAG	20	7.007261E-4	45.0	1
CCACAGG	25	3.869248E-5	45.0	3
CGTTTTA	460	0.0	44.02174	1
>>END_MODULE