SRR2936169.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936169.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102286
Sequences flagged as poor quality	0
Sequence length	51
%GC	30

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5307	5.188393328510255	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4794	4.686858416596602	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3037	2.9691257845648478	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2164	2.115636548501261	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	1594	1.558375535263868	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG	1276	1.2474825489314274	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1179	1.152650411591029	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC	1164	1.1379856480847819	No Hit
GAATCTATCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	1042	1.0187122382339713	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG	912	0.891617621179829	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC	746	0.7293275717106935	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	556	0.5435739006315624	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	430	0.4203898871790861	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	425	0.41550163267700374	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCT	372	0.3636861349549303	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCT	364	0.35586492775159845	No Hit
GAATATGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	323	0.3157812408345228	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG	318	0.3108929863324404	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	275	0.2688539976145318	TruSeq Adapter, Index 14 (95% over 21bp)
GAATCTATCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTT	260	0.2541892341082846	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTT	257	0.25125628140703515	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	249	0.24343507420370333	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTC	232	0.2268150088966232	No Hit
GAATGAATGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCT	226	0.2209491034941243	No Hit
GAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	190	0.18575367107913107	No Hit
GAACTGACTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCT	181	0.17695481297538276	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC	163	0.15935709676788612	No Hit
GAATGAACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTC	161	0.15740179496705317	No Hit
GCTGTCACTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	151	0.14762528596288838	TruSeq Adapter, Index 14 (95% over 21bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	151	0.14762528596288838	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTT	149	0.14566998416205543	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	147	0.14371468236122245	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	145	0.1417593805603895	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTA	128	0.12513931525330935	No Hit
GAATGCAGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTT	114	0.11145220264747863	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTCA	25	3.8658407E-5	45.0	1
TGCACGG	25	3.8658407E-5	45.0	2
AATCCGG	20	7.0031435E-4	45.0	19
GCAAGTA	20	7.0031435E-4	45.0	43
AAATCCG	20	7.0031435E-4	45.0	18
AGGACCA	20	7.0031435E-4	45.0	7
ATGAACT	40	6.710252E-9	45.0	3
GGCGACT	35	1.1974771E-7	45.0	10
AGGCGAT	20	7.0031435E-4	45.0	7
ATAACGG	20	7.0031435E-4	45.0	2
CGAGGGC	20	7.0031435E-4	45.0	4
AGAACCC	20	7.0031435E-4	45.0	10
ATAATAC	75	0.0	45.0	3
TATCTTA	25	3.8658407E-5	45.0	6
GCTGTAT	20	7.0031435E-4	45.0	2
GAACCAA	20	7.0031435E-4	45.0	13
ACGGGAG	20	7.0031435E-4	45.0	5
GGGACTA	25	3.8658407E-5	45.0	7
TTAGGCA	20	7.0031435E-4	45.0	39
GAATATG	50	2.1827873E-11	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content