##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936169.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102286
Sequences flagged as poor quality	0
Sequence length	51
%GC	30
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29469331091254	31.0	31.0	34.0	28.0	34.0
2	31.381977983301724	31.0	31.0	34.0	30.0	34.0
3	30.066157636431182	31.0	30.0	34.0	25.0	34.0
4	34.627524783450326	35.0	35.0	37.0	32.0	37.0
5	31.27814168116849	35.0	35.0	37.0	0.0	37.0
6	33.37219169778855	35.0	35.0	37.0	17.0	37.0
7	21.410799131846	32.0	0.0	37.0	0.0	37.0
8	28.119644917192968	35.0	17.0	37.0	17.0	37.0
9	34.24558590618462	35.0	32.0	39.0	28.0	39.0
10	36.16788221261952	37.0	35.0	39.0	32.0	39.0
11	36.661566587802824	38.0	35.0	39.0	33.0	39.0
12	36.98801399996089	39.0	37.0	39.0	33.0	39.0
13	36.82080636646266	39.0	37.0	39.0	32.0	39.0
14	38.4431984826858	40.0	38.0	41.0	34.0	41.0
15	38.387051991474884	40.0	38.0	41.0	33.0	41.0
16	38.43152533093483	40.0	38.0	41.0	34.0	41.0
17	38.25886240541227	40.0	38.0	41.0	33.0	41.0
18	37.852286725456075	39.0	37.0	40.0	33.0	41.0
19	37.41644995405041	39.0	36.0	40.0	33.0	41.0
20	37.16533054376943	39.0	35.0	40.0	33.0	41.0
21	37.08204446356295	38.0	35.0	40.0	33.0	41.0
22	36.838697377940285	38.0	35.0	40.0	33.0	41.0
23	36.611413096611464	37.0	35.0	40.0	33.0	41.0
24	36.1730051033377	36.0	34.0	40.0	32.0	41.0
25	36.02508652210469	36.0	34.0	40.0	32.0	41.0
26	35.81484269597012	35.0	34.0	40.0	31.0	41.0
27	35.80884969595057	36.0	34.0	40.0	31.0	41.0
28	35.71108460590892	35.0	34.0	40.0	31.0	41.0
29	35.598576540288995	35.0	34.0	40.0	31.0	41.0
30	35.4826369200086	35.0	34.0	40.0	31.0	41.0
31	35.32100189664275	35.0	34.0	40.0	30.0	41.0
32	34.91931447118863	35.0	33.0	40.0	26.0	41.0
33	34.49635336214145	35.0	33.0	40.0	25.0	41.0
34	33.75684844455742	35.0	33.0	40.0	21.0	41.0
35	32.87858553467728	35.0	33.0	40.0	16.0	41.0
36	32.54896075709286	35.0	33.0	40.0	15.0	41.0
37	32.375134426998805	35.0	32.0	40.0	15.0	41.0
38	32.1262929433158	35.0	31.0	40.0	10.0	41.0
39	31.97443442895411	35.0	31.0	40.0	10.0	41.0
40	31.427360538099055	35.0	30.0	39.0	10.0	41.0
41	31.352951528068356	35.0	30.0	39.0	10.0	41.0
42	31.010881254521635	35.0	29.0	39.0	9.0	41.0
43	30.91300862288094	35.0	27.0	39.0	8.0	41.0
44	30.8335744872221	35.0	27.0	39.0	8.0	41.0
45	30.72124239876425	35.0	26.0	39.0	8.0	41.0
46	30.339186203390494	35.0	25.0	39.0	8.0	41.0
47	30.10499970670473	35.0	24.0	39.0	8.0	41.0
48	29.798946092329352	35.0	23.0	39.0	7.0	41.0
49	29.628766400093856	35.0	23.0	39.0	7.0	40.0
50	29.474424652445105	35.0	23.0	38.0	7.0	40.0
51	27.6107971765442	33.0	19.0	36.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	2.0
13	4.0
14	9.0
15	12.0
16	13.0
17	40.0
18	77.0
19	134.0
20	250.0
21	392.0
22	651.0
23	1108.0
24	2032.0
25	2868.0
26	3697.0
27	4176.0
28	4091.0
29	3764.0
30	3751.0
31	4285.0
32	5664.0
33	7283.0
34	9381.0
35	12225.0
36	8513.0
37	10954.0
38	10346.0
39	6557.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.93467336683417	17.608470367401207	12.856109340476701	32.600746925287915
2	41.38396261462957	37.407856402635744	12.437674755098449	8.770506227636234
3	17.004282110943826	37.531040416088224	37.66302328764445	7.801654185323505
4	14.873980798936316	24.43345130320865	50.93072365719649	9.761844240658546
5	18.454138396261463	34.19040728936512	34.75744481160667	12.598009502766752
6	12.725104119820893	37.019728995170404	41.94122362786696	8.313943257141739
7	24.631914435993195	44.953366052050136	26.71626615568113	3.6984533562755413
8	37.92014547445398	21.990301703067868	35.101577928553276	4.987974893924877
9	35.180767651487	16.940734802416753	33.666386406741886	14.212111139354358
10	20.858181960385586	27.388889975167668	40.296814813366446	11.456113251080305
11	16.768668243943452	26.132608568132493	43.164264904287975	13.934458283636078
12	15.707917016991574	24.49993156443697	46.25266409870363	13.53948731986782
13	11.845218309446064	27.279393074321025	50.45265236689283	10.422736249340085
14	11.269381929100756	32.08454724986802	42.14164206245234	14.504428758578886
15	9.072600355864928	28.959974972136948	51.52611305555013	10.441311616447999
16	10.12161977201181	31.55172750914104	41.30183993899458	17.024812779852567
17	10.323015857497603	29.18287937743191	44.84875740570557	15.645347359364917
18	10.175390571534717	31.738458831120585	43.76258725534286	14.323563342001838
19	10.416870343937587	31.9173689458968	40.77781905637135	16.887941653794265
20	11.184326300764523	31.511643822223963	46.08548579473242	11.2185440822791
21	12.174686662886417	33.6497663414348	42.83479655084763	11.34075044483116
22	10.645640654635043	27.85229650196508	42.47599867039477	19.026064173005103
23	11.380834131748236	32.84222669769079	43.8183133566666	11.958625813894376
24	15.317834307725398	27.80439160784467	40.30659132237061	16.571182762059326
25	11.015192694992471	37.00799718436541	39.05226521713627	12.924544903505856
26	11.712257786989422	29.23958312965606	41.27837631738459	17.76978276596993
27	16.070625501046088	30.621003851944543	38.20464188647518	15.103728760534189
28	10.262401501671784	29.188745282834404	44.00602232954657	16.542830885947247
29	14.951215220069217	32.73468509864498	37.728525897972354	14.585573783313455
30	16.484171831922257	30.94265099818157	40.300725416968106	12.272451752928063
31	15.759732514713646	28.880785249203218	37.1976614590462	18.161820777036937
32	16.4274680796981	34.48076960678881	38.09416733472811	10.997594978784976
33	17.15288504780713	27.73595604481552	37.97489392487731	17.136264982500048
34	18.183329096846098	31.744324736523083	38.427546291770135	11.644799874860686
35	16.385429090980193	28.683299767319088	40.728936511350526	14.202334630350194
36	14.634456328334277	31.529241538431457	39.86371546448194	13.97258666875232
37	15.284594177111238	32.53133371135834	40.480613182644746	11.703458928885674
38	15.646325010265336	30.833154097334926	35.36163306806406	18.158887824335686
39	19.719218661400387	31.929100756701796	36.192636333418065	12.159044248479752
40	17.312242144575016	29.759693408677627	40.98312574545881	11.944938701288542
41	15.248421093795827	33.536358836986494	37.347242046809924	13.86797802240776
42	13.721330387345287	30.454803198873748	42.388010089357294	13.435856324423675
43	17.850927790704496	30.407875955653758	35.651017734587334	16.090178519054415
44	16.283753397336877	30.104804176524645	35.981463738928106	17.62997868721037
45	14.61979156482803	29.68148133664431	35.09180141954911	20.60692567897855
46	19.668380814578732	32.43845687581878	33.75633028958019	14.136832020022291
47	13.161136421406644	28.6842774182195	43.44778366540876	14.7068024949651
48	15.405822888762879	31.055080851729468	34.74962360440334	18.789472655104316
49	12.393680464579708	28.30201591615666	42.184658702070664	17.119644917192968
50	14.516160569383885	27.70467121600219	39.74737500733238	18.031793207281545
51	14.653031695442193	27.121991279353967	36.43704905852218	21.787927966681657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5570.0
1	4877.5
2	4185.0
3	9061.0
4	13937.0
5	8909.0
6	3881.0
7	3132.5
8	2384.0
9	2185.5
10	1987.0
11	1858.5
12	1730.0
13	1621.5
14	1513.0
15	1378.5
16	1244.0
17	1134.5
18	1025.0
19	953.0
20	881.0
21	852.5
22	824.0
23	798.5
24	773.0
25	812.5
26	976.0
27	1100.0
28	1202.5
29	1305.0
30	1438.0
31	1571.0
32	1694.5
33	1818.0
34	1890.5
35	1963.0
36	2016.5
37	2070.0
38	2224.0
39	2378.0
40	2525.0
41	2672.0
42	3253.0
43	3834.0
44	5532.5
45	7231.0
46	10147.5
47	13064.0
48	9234.5
49	5405.0
50	4904.5
51	4404.0
52	3541.0
53	2678.0
54	2434.5
55	2191.0
56	2036.5
57	1882.0
58	1761.0
59	1640.0
60	1489.5
61	1339.0
62	1192.0
63	1045.0
64	877.0
65	709.0
66	586.5
67	464.0
68	357.5
69	251.0
70	213.5
71	176.0
72	143.0
73	110.0
74	105.5
75	77.0
76	53.0
77	39.5
78	26.0
79	19.5
80	13.0
81	8.5
82	4.0
83	2.0
84	0.0
85	1.0
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	102286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.51117454979176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.90188180005134	41.04569540308546
2	9.395178010781352	9.303325968363216
3	3.6510475287798902	5.423029544610211
4	1.6527456904211837	3.2731752145943727
5	0.8273601484904133	2.048178636372524
6	0.39689591848824124	1.179046985902274
7	0.22905436091858697	0.7938525311381811
8	0.14809549197322433	0.5865905402498875
9	0.10465414766107853	0.46633947949866067
>10	0.5706612957368244	5.619537375593922
>50	0.053314377110360765	1.731419744637585
>100	0.0454159508717888	5.2900690221535696
>500	0.005923819678928973	2.164519093522085
>1k	0.01579685247714393	15.886827131767788
>5k	0.001974606559642991	5.188393328510255
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5307	5.188393328510255	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4794	4.686858416596602	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3037	2.9691257845648478	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2164	2.115636548501261	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	1594	1.558375535263868	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG	1276	1.2474825489314274	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1179	1.152650411591029	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC	1164	1.1379856480847819	No Hit
GAATCTATCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	1042	1.0187122382339713	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG	912	0.891617621179829	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC	746	0.7293275717106935	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	556	0.5435739006315624	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	430	0.4203898871790861	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	425	0.41550163267700374	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCT	372	0.3636861349549303	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCT	364	0.35586492775159845	No Hit
GAATATGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	323	0.3157812408345228	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG	318	0.3108929863324404	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	275	0.2688539976145318	TruSeq Adapter, Index 14 (95% over 21bp)
GAATCTATCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTT	260	0.2541892341082846	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTT	257	0.25125628140703515	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	249	0.24343507420370333	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTC	232	0.2268150088966232	No Hit
GAATGAATGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCT	226	0.2209491034941243	No Hit
GAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	190	0.18575367107913107	No Hit
GAACTGACTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCT	181	0.17695481297538276	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC	163	0.15935709676788612	No Hit
GAATGAACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTC	161	0.15740179496705317	No Hit
GCTGTCACTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	151	0.14762528596288838	TruSeq Adapter, Index 14 (95% over 21bp)
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	151	0.14762528596288838	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTT	149	0.14566998416205543	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	147	0.14371468236122245	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	145	0.1417593805603895	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTA	128	0.12513931525330935	No Hit
GAATGCAGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTT	114	0.11145220264747863	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11536280624914455	0.0
2	0.0	0.0	0.0	0.7723442113290186	0.0
3	0.0	0.0	0.0	1.015779285532722	0.0
4	0.0	0.0	0.0	1.7323973955380012	0.0
5	0.0	0.0	0.0	4.269401482118765	0.0
6	0.0	0.0	0.0	4.975265432219463	0.0
7	0.0	0.0	0.0	5.884480769606789	0.0
8	0.0	0.0	0.0	6.951097901961168	0.0
9	0.0	0.0	0.0	7.350957120231508	0.0
10	0.0	0.0	0.0	10.717009170365445	0.0
11	0.0	0.0	0.0	11.192147507967855	0.0
12	0.0	0.0	0.0	13.983340828656903	0.0
13	0.0	0.0	0.0	14.277613749682263	0.0
14	0.0	0.0	0.0	14.479987486068474	0.0
15	0.0	0.0	0.0	14.892556166044228	0.0
16	0.0	0.0	0.0	15.133058287546683	0.0
17	0.0	0.0	0.0	15.344230882036642	0.0
18	0.0	0.0	0.0	15.609174276049508	0.0
19	0.0	0.0	0.0	15.763643118315311	0.0
20	0.0	0.0	0.0	15.884871829966956	0.0
21	0.0	0.0	0.0	16.046184228535676	0.0
22	0.0	0.0	0.0	16.221183739710224	0.0
23	0.0	0.0	0.0	16.388362043681443	0.0
24	0.0	0.0	0.0	16.518389613436835	0.0
25	0.0	0.0	0.0	16.628864165183895	0.0
26	0.0	0.0	0.0	16.754981131337622	0.0
27	0.0	0.0	0.0	16.858612126781768	0.0
28	0.0	0.0	0.0	16.971041980329662	0.0
29	0.0	0.0	0.0	17.070762372172144	0.0
30	0.0	0.0	0.0	17.215454705433785	0.0
31	0.0	0.0	0.0	17.326906908081263	0.0
32	0.0	0.0	0.0	17.45009092153374	0.0
33	0.0	0.0	0.0	17.582073793089965	0.0
34	0.0	0.0	0.0	17.715034315546603	0.0
35	0.0	0.0	0.0	17.881234968617406	0.0
36	0.0	0.0	0.0	17.997575425766968	0.0
37	0.0	0.0	0.0	18.139334806327355	0.0
38	0.0	0.0	0.0	18.272295328783997	0.0
39	0.0	0.0	0.0	18.442406585456464	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTCA	25	3.8658407E-5	45.0	1
TGCACGG	25	3.8658407E-5	45.0	2
AATCCGG	20	7.0031435E-4	45.0	19
GCAAGTA	20	7.0031435E-4	45.0	43
AAATCCG	20	7.0031435E-4	45.0	18
AGGACCA	20	7.0031435E-4	45.0	7
ATGAACT	40	6.710252E-9	45.0	3
GGCGACT	35	1.1974771E-7	45.0	10
AGGCGAT	20	7.0031435E-4	45.0	7
ATAACGG	20	7.0031435E-4	45.0	2
CGAGGGC	20	7.0031435E-4	45.0	4
AGAACCC	20	7.0031435E-4	45.0	10
ATAATAC	75	0.0	45.0	3
TATCTTA	25	3.8658407E-5	45.0	6
GCTGTAT	20	7.0031435E-4	45.0	2
GAACCAA	20	7.0031435E-4	45.0	13
ACGGGAG	20	7.0031435E-4	45.0	5
GGGACTA	25	3.8658407E-5	45.0	7
TTAGGCA	20	7.0031435E-4	45.0	39
GAATATG	50	2.1827873E-11	45.0	1
>>END_MODULE