##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936168.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	217766
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41233250369663	31.0	31.0	34.0	30.0	34.0
2	31.55896696453992	31.0	31.0	34.0	30.0	34.0
3	30.425589853328802	31.0	30.0	34.0	25.0	34.0
4	34.82211180808758	35.0	35.0	37.0	32.0	37.0
5	31.650018827548838	35.0	35.0	37.0	10.0	37.0
6	33.61558278151777	35.0	35.0	37.0	19.0	37.0
7	21.302172974660873	32.0	0.0	37.0	0.0	37.0
8	28.27873497240157	35.0	17.0	37.0	17.0	37.0
9	34.70282321390851	37.0	32.0	39.0	28.0	39.0
10	36.2372776282799	37.0	35.0	39.0	32.0	39.0
11	36.69456205284572	38.0	35.0	39.0	33.0	39.0
12	37.025031455782816	39.0	37.0	39.0	34.0	39.0
13	36.82563852943068	39.0	37.0	39.0	33.0	39.0
14	38.170182673144566	40.0	38.0	41.0	33.0	41.0
15	38.26907322538872	40.0	38.0	41.0	33.0	41.0
16	38.35906431674366	40.0	38.0	41.0	33.0	41.0
17	38.2947567572532	40.0	38.0	41.0	33.0	41.0
18	38.02376863238522	39.0	37.0	41.0	33.0	41.0
19	37.75688583158069	39.0	37.0	40.0	33.0	41.0
20	37.52421406463819	39.0	35.0	40.0	33.0	41.0
21	37.41336572284011	39.0	35.0	40.0	33.0	41.0
22	37.28890644085853	39.0	35.0	40.0	33.0	41.0
23	37.154840516885095	38.0	35.0	40.0	33.0	41.0
24	36.881110917223076	38.0	35.0	40.0	33.0	41.0
25	36.71282936730252	38.0	35.0	40.0	32.0	41.0
26	36.56872514533949	38.0	35.0	40.0	32.0	41.0
27	36.51410229328729	38.0	35.0	40.0	32.0	41.0
28	36.321661783749526	38.0	35.0	40.0	31.0	41.0
29	36.27660424492345	38.0	35.0	40.0	31.0	41.0
30	36.03116188936749	38.0	34.0	40.0	31.0	41.0
31	35.661407198552574	38.0	34.0	40.0	30.0	41.0
32	35.31847487670252	37.0	34.0	40.0	27.0	41.0
33	34.75645876766805	37.0	33.0	40.0	24.0	41.0
34	34.02045314695591	37.0	33.0	40.0	21.0	41.0
35	33.27251269711525	37.0	33.0	40.0	15.0	41.0
36	32.644466996684514	37.0	31.0	40.0	12.0	41.0
37	32.42400558397546	37.0	31.0	40.0	10.0	41.0
38	31.97139590202327	36.0	30.0	40.0	9.0	41.0
39	31.829904576471993	36.0	30.0	40.0	9.0	41.0
40	31.658381014483435	36.0	29.0	40.0	9.0	41.0
41	31.473811338776486	36.0	28.0	40.0	8.0	41.0
42	31.352020058227637	35.0	28.0	40.0	8.0	41.0
43	31.158266212356384	35.0	27.0	40.0	8.0	41.0
44	30.94813699108217	35.0	26.0	40.0	8.0	41.0
45	30.823567499058623	35.0	26.0	40.0	8.0	41.0
46	30.581119182976224	35.0	25.0	40.0	8.0	41.0
47	30.515117144090446	35.0	25.0	40.0	8.0	41.0
48	30.302016843768083	35.0	24.0	40.0	7.0	41.0
49	30.22203190580715	35.0	24.0	39.0	7.0	41.0
50	30.03840360754204	35.0	24.0	39.0	7.0	41.0
51	28.24624597044534	33.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	9.0
11	7.0
12	4.0
13	10.0
14	2.0
15	11.0
16	24.0
17	54.0
18	126.0
19	204.0
20	462.0
21	835.0
22	1595.0
23	2745.0
24	4721.0
25	7002.0
26	8641.0
27	8868.0
28	8129.0
29	7330.0
30	7164.0
31	7786.0
32	9375.0
33	12257.0
34	15286.0
35	18659.0
36	20760.0
37	30364.0
38	28264.0
39	17064.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.03026184069139	15.547422462643388	19.172414426494495	30.24990127017073
2	44.91702102256551	22.674338510143915	19.53748519052561	12.871155276764966
3	25.31157297282404	22.055784649577987	40.45626957376267	12.17637280383531
4	21.35089958946759	18.793567407216923	45.09703075778588	14.758502245529606
5	19.429111982586814	29.800336140628016	36.839084154551216	13.93146772223396
6	19.216957651791372	27.97544152898065	40.856240184418134	11.951360634809841
7	36.57733530486853	40.38922513156324	20.398501143429186	2.6349384201390484
8	62.4440913641248	5.909554292221926	27.661802117869637	3.984552225783639
9	58.386065777026715	5.072876390253759	28.923247889936903	7.61780994278262
10	31.774014308937115	19.743210602206037	35.455488919298695	13.027286169558149
11	23.74107987472792	20.261657007981043	40.50999696922384	15.487266148067192
12	20.84071893684046	17.715805038435754	44.900948724777976	16.542527299945814
13	18.61906817409513	18.173635921126348	47.79947282863257	15.407823076145954
14	16.278023199213834	20.24374787616065	45.92773894914725	17.550489975478264
15	14.654721122672962	19.936078175656437	49.651001533756414	15.758199167914183
16	18.065721921695765	20.408144522101708	43.58853080829881	17.937602747903714
17	18.14929787019094	19.781324908387905	44.218564881570124	17.850812339851032
18	18.568096029683236	19.86352323135843	44.88487642699044	16.68350431196789
19	17.520641422444275	22.074612198414812	42.634754736735765	17.76999164240515
20	18.187871384881017	22.28447048666918	44.11799821827099	15.409659910178815
21	18.192004261454954	23.321822506727404	43.34469109043652	15.141482141381116
22	17.333743559600673	19.603611215708604	44.09366016733558	18.96898505735514
23	16.409816041071608	22.643571540093497	44.02523809961151	16.921374319223386
24	18.147461036158077	20.763571907460303	42.07497956522138	19.013987491160236
25	17.064188165278328	24.06160741346216	41.342541994618074	17.53166242664144
26	17.556459686085063	20.605144972126045	42.01757850169448	19.820816840094412
27	18.18098325725779	20.820972970987206	42.22192628785026	18.77611748390474
28	15.593802521973126	20.84944389849655	44.77604401054342	18.780709568986893
29	20.06419734944849	20.805819090216104	41.79256633266901	17.337417227666393
30	20.303904190736848	20.571622751026332	42.68251242159015	16.441960636646677
31	20.515140104515854	19.60269279869217	42.58010892425815	17.302058172533822
32	21.163542518115776	20.524783483188376	41.474794044984066	16.836879953711783
33	20.49585334717082	19.89658624394993	41.10007990228043	18.507480506598824
34	20.40676689657706	19.82770496771764	43.10682108318103	16.658707052524267
35	19.710606798122758	21.58968801373952	39.81337766226133	18.8863275258764
36	21.77566745956669	22.30513486953886	39.60030491444945	16.318892756444992
37	19.31385064702479	23.213449298788607	40.03379774620464	17.438902307981962
38	20.526620317221237	23.9362434907194	37.518253538201556	18.01888265385781
39	20.076595979170303	22.133850095974577	39.24809198864836	18.541461936206755
40	21.635608864561043	22.302838826997785	36.75504899754783	19.306503310893344
41	18.33022602242774	23.372794651139298	37.78229843042532	20.514680896007643
42	19.177465720084864	23.04537898478183	37.9292451530542	19.84791014207911
43	20.20379673594592	22.787303803164864	36.87582083520844	20.133078625680774
44	20.61800281035607	22.778578841508775	36.327525876399434	20.275892471735716
45	19.731730389500658	23.156966652278133	35.09225498930044	22.01904796892077
46	21.293957734448902	24.159878034220217	34.9191333817033	19.627030849627584
47	18.285682797130864	23.731895704563613	38.69658257028186	19.285838928023658
48	19.427734357062167	24.635158840222992	35.63412102899443	20.302985773720415
49	19.241754911234995	22.573771846844778	38.118439058438874	20.066034183481353
50	18.899185364106426	22.6587254208646	37.026900434411246	21.41518878061773
51	18.812854164561962	21.877611748390475	35.91607505303858	23.39345903400898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	124.0
1	308.0
2	492.0
3	9472.0
4	18452.0
5	12197.0
6	5942.0
7	5101.5
8	4261.0
9	3923.5
10	3586.0
11	3434.5
12	3283.0
13	3106.5
14	2930.0
15	2792.0
16	2654.0
17	2464.0
18	2274.0
19	2129.5
20	1985.0
21	1883.0
22	1781.0
23	1816.5
24	1852.0
25	2010.0
26	2445.5
27	2723.0
28	2932.0
29	3141.0
30	3297.0
31	3453.0
32	3930.5
33	4408.0
34	4789.0
35	5170.0
36	5487.0
37	5804.0
38	6177.0
39	6550.0
40	6900.0
41	7250.0
42	8161.0
43	9072.0
44	10800.5
45	12529.0
46	15617.5
47	18706.0
48	17091.5
49	15477.0
50	15021.0
51	14565.0
52	12966.0
53	11367.0
54	10516.5
55	9666.0
56	9155.0
57	8644.0
58	7981.5
59	7319.0
60	6607.5
61	5896.0
62	5146.0
63	4396.0
64	3920.0
65	3444.0
66	2909.5
67	2375.0
68	1955.0
69	1535.0
70	1303.0
71	1071.0
72	850.5
73	630.0
74	510.5
75	297.0
76	203.0
77	154.5
78	106.0
79	73.5
80	41.0
81	31.5
82	22.0
83	19.0
84	16.0
85	10.0
86	4.0
87	2.5
88	1.0
89	2.0
90	3.0
91	2.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	217766.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.070060255875966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.17920932406516	40.85053527209504
2	12.631920757705231	13.912812745485548
3	5.796092569101371	9.575735010871423
4	3.1396277909501378	6.915979665145873
5	1.6727491871968856	4.605919926595002
6	0.9391523419106809	3.103150563508093
7	0.5675506778000567	2.1878535017298657
8	0.3010032654489186	1.32610143723899
9	0.17664641142546283	0.8755135669066043
>10	0.543745553918584	4.8535499899129295
>50	0.027631308931525288	1.046683219599237
>100	0.014802486927602831	1.4059031615281548
>500	0.006907827232881322	2.766700036853435
>1k	0.0019736649236803774	2.8840850708271812
>5k	9.868324618401887E-4	3.6894768317026068
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6789	3.117566562273266	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3811	1.7500436248082805	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1496	0.6869759282899994	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG	882	0.40502190424584184	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC	878	0.4031850702129809	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC	834	0.3829798958515103	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	704	0.3232827897835291	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG	658	0.30215919840562805	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC	605	0.27782114747022035	No Hit
GAATCTATCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC	530	0.2433805093540773	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	295	0.13546650992349588	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	285	0.13087442484134348	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT	251	0.1152613355620253	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06888127623228603	0.0
2	0.0	0.0	0.0	0.4628821762809621	0.0
3	0.0	0.0	0.0	0.5597751715143778	0.0
4	0.0	0.0	0.0	0.8362186934599525	0.0
5	0.0	0.0	0.0	1.6660084678048914	0.0
6	0.0	0.0	0.0	2.0962868400025716	0.0
7	0.0	0.0	0.0	2.6404489222376313	0.0
8	0.0	0.0	0.0	3.58504082363638	0.0
9	0.0	0.0	0.0	4.227473526629502	0.0
10	0.0	0.0	0.0	6.32789324320601	0.0
11	0.0	0.0	0.0	7.334478293213817	0.0
12	0.0	0.0	0.0	9.063398326644196	0.0
13	0.0	0.0	0.0	9.514800290219776	0.0
14	0.0	0.0	0.0	9.79629510575572	0.0
15	0.0	0.0	0.0	10.09432142758741	0.0
16	0.0	0.0	0.0	10.413471340797003	0.0
17	0.0	0.0	0.0	10.769817143172029	0.0
18	0.0	0.0	0.0	11.17392063040144	0.0
19	0.0	0.0	0.0	11.425107684395176	0.0
20	0.0	0.0	0.0	11.657467189552088	0.0
21	0.0	0.0	0.0	11.893500362774722	0.0
22	0.0	0.0	0.0	12.200710854770717	0.0
23	0.0	0.0	0.0	12.493226674503825	0.0
24	0.0	0.0	0.0	12.71364675844714	0.0
25	0.0	0.0	0.0	12.916157710570062	0.0
26	0.0	0.0	0.0	13.117291037168336	0.0
27	0.0	0.0	0.0	13.368018882653859	0.0
28	0.0	0.0	0.0	13.597163928253263	0.0
29	0.0	0.0	0.0	13.82217609727873	0.0
30	0.0	0.0	0.0	14.144999678554043	0.0
31	0.0	0.0	0.0	14.392972272990274	0.0
32	0.0	0.0	0.0	14.658853999246897	0.0
33	0.0	0.0	0.0	14.910959470257065	0.0
34	0.0	0.0	0.0	15.153421562594712	0.0
35	0.0	0.0	0.0	15.41792566332669	0.0
36	0.0	0.0	0.0	15.647989125942525	0.0
37	0.0	0.0	0.0	15.894124886345894	0.0
38	0.0	0.0	0.0	16.17056840829147	0.0
39	0.0	0.0	0.0	16.537476006355444	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.1573742E-6	45.000004	4
ATCTATC	140	0.0	45.000004	3
CAATTGG	30	2.1573742E-6	45.000004	2
AATCTAT	140	0.0	45.000004	2
CATACGA	30	2.1573742E-6	45.000004	18
TAGGCGA	30	2.1573742E-6	45.000004	6
GTGCATA	20	7.020683E-4	45.0	1
GTCGACA	20	7.020683E-4	45.0	9
TTCAGAC	20	7.020683E-4	45.0	26
GCGTTAG	25	3.8803617E-5	45.0	2
TACGAAA	25	3.8803617E-5	45.0	20
ATACGAA	25	3.8803617E-5	45.0	19
TCGACAC	20	7.020683E-4	45.0	10
CGGTTTA	115	0.0	44.999996	1
CGTTATT	545	0.0	44.999996	1
CGTTTTA	1345	0.0	44.665424	1
CGTTTTT	2370	0.0	43.860756	1
GAATCTA	145	0.0	43.448273	1
GTTATTT	590	0.0	42.711864	2
AAACGGC	180	0.0	42.5	6
>>END_MODULE