##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936167.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	148030
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.461656420995745	31.0	31.0	34.0	30.0	34.0
2	31.63915422549483	31.0	31.0	34.0	30.0	34.0
3	30.56205498885361	31.0	30.0	34.0	26.0	34.0
4	34.907295818415186	37.0	35.0	37.0	32.0	37.0
5	31.546152806863475	35.0	35.0	37.0	0.0	37.0
6	33.596581773964736	36.0	35.0	37.0	17.0	37.0
7	21.340214821320004	32.0	0.0	37.0	0.0	37.0
8	28.397439708167262	35.0	17.0	37.0	17.0	37.0
9	34.82313044653111	37.0	32.0	39.0	30.0	39.0
10	36.33407417415389	37.0	35.0	39.0	32.0	39.0
11	36.741701006552724	39.0	37.0	39.0	33.0	39.0
12	37.05898128757684	39.0	37.0	39.0	34.0	39.0
13	36.795703573599944	39.0	37.0	39.0	33.0	39.0
14	38.072431263932984	40.0	37.0	41.0	33.0	41.0
15	38.2341417280281	40.0	38.0	41.0	33.0	41.0
16	38.331628723907315	40.0	38.0	41.0	33.0	41.0
17	38.294534891576035	40.0	38.0	41.0	33.0	41.0
18	38.05696142673782	39.0	37.0	41.0	33.0	41.0
19	37.85808957643721	39.0	37.0	41.0	33.0	41.0
20	37.558616496656086	39.0	35.0	40.0	33.0	41.0
21	37.40480308045667	39.0	35.0	40.0	33.0	41.0
22	37.258751604404516	39.0	35.0	40.0	33.0	41.0
23	37.130764034317366	38.0	35.0	40.0	33.0	41.0
24	36.87603864081605	38.0	35.0	40.0	33.0	41.0
25	36.69884482875093	38.0	35.0	40.0	32.0	41.0
26	36.59181247044518	38.0	35.0	40.0	32.0	41.0
27	36.50593123015605	38.0	35.0	40.0	32.0	41.0
28	36.37114098493549	38.0	35.0	40.0	32.0	41.0
29	36.26764169425117	38.0	35.0	40.0	31.0	41.0
30	36.05349591299061	38.0	35.0	40.0	31.0	41.0
31	35.79002229277849	38.0	34.0	40.0	30.0	41.0
32	35.52623792474498	38.0	34.0	40.0	29.0	41.0
33	35.04445044923327	37.0	34.0	40.0	25.0	41.0
34	34.39375126663514	37.0	33.0	40.0	22.0	41.0
35	33.770850503276364	37.0	33.0	40.0	18.0	41.0
36	33.32193474295751	37.0	33.0	40.0	15.0	41.0
37	33.058163885698846	37.0	33.0	40.0	14.0	41.0
38	32.78995473890428	37.0	32.0	40.0	10.0	41.0
39	32.61293656691211	37.0	32.0	40.0	10.0	41.0
40	32.43569546713504	36.0	31.0	40.0	10.0	41.0
41	32.200817401878	36.0	31.0	40.0	10.0	41.0
42	32.079882456258865	36.0	31.0	40.0	10.0	41.0
43	31.98301695602243	36.0	30.0	40.0	10.0	41.0
44	31.794399783827604	35.0	30.0	40.0	9.0	41.0
45	31.73321623995136	35.0	30.0	40.0	9.0	41.0
46	31.5759913531041	35.0	29.0	40.0	9.0	41.0
47	31.51458488144295	35.0	29.0	40.0	9.0	41.0
48	31.356448017293793	35.0	29.0	40.0	8.0	41.0
49	31.233000067553874	35.0	28.0	40.0	8.0	41.0
50	31.053374316017024	35.0	27.0	39.0	8.0	41.0
51	29.330872120516112	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	7.0
10	9.0
11	6.0
12	3.0
13	5.0
14	9.0
15	7.0
16	19.0
17	39.0
18	67.0
19	178.0
20	279.0
21	545.0
22	937.0
23	1645.0
24	2623.0
25	3994.0
26	5060.0
27	5193.0
28	5040.0
29	4502.0
30	4511.0
31	5107.0
32	6386.0
33	8828.0
34	11023.0
35	13933.0
36	14753.0
37	21182.0
38	20044.0
39	12091.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.409376477740995	17.833547253935013	21.634803755995407	30.12227251232858
2	44.98007160710667	20.862662973721545	20.220901168682023	13.936364250489767
3	26.392623116935752	19.75477943660069	40.44990880226981	13.402688644193745
4	22.37181652367763	21.21394311963791	41.37201918530028	15.04222117138418
5	18.27534959129906	32.08809025197595	35.867729514287646	13.768830642437344
6	20.3229075187462	27.27217455921097	40.44585556981693	11.959062352225901
7	38.02134702425184	40.92008376680403	18.215226643247988	2.8433425656961426
8	65.04559886509492	4.730122272512329	26.46490576234547	3.759373100047288
9	59.65277308653651	6.661487536310208	28.55772478551645	5.1280145916368305
10	33.643180436398026	21.5713031142336	33.528338850233055	11.25717759913531
11	24.64297777477538	20.083766804026208	40.33371613862055	14.939539282577854
12	22.537323515503612	18.355738701614538	43.51955684658515	15.587380936296697
13	20.428967101263257	18.71647638992096	44.71053164899007	16.144024859825713
14	16.022427886239278	20.38505708302371	45.524555833277034	18.067959197459974
15	15.294872660947107	21.126123083158816	47.1688171316625	16.410187124231573
16	19.94595690062825	20.872796054853744	43.01628048368574	16.164966560832266
17	19.943930284401812	20.460041883402013	41.677362696750656	17.91866513544552
18	19.706140647166116	20.095926501384852	43.7566709450787	16.44126190637033
19	19.210970749172464	21.831385529960144	41.852327230966694	17.105316489900694
20	18.931973248665813	23.424981422684592	41.880699858136865	15.762345470512734
21	19.577788286158214	22.896034587583596	42.348848206444636	15.17732891981355
22	18.442207660609338	20.64784165371884	43.25947443085861	17.650476254813213
23	17.249206241977976	21.05451597649125	44.36870904546375	17.327568736067015
24	18.116597986894547	20.8505032763629	43.04059987840302	17.992298858339524
25	18.143619536580424	22.4866581098426	40.84577450516787	18.523947848409104
26	16.591907045869082	21.58684050530298	41.36256164291022	20.45869080591772
27	16.040667432277242	21.890832939269067	43.10545159764912	18.963048030804565
28	15.3928257785584	22.627170168209148	42.404242383300684	19.575761669931772
29	17.255961629399447	22.96629061676687	41.57738296291292	18.20036479092076
30	18.569208944132946	22.031344997635614	41.68276700668783	17.716679051543608
31	18.504357224886846	22.737958521921232	40.980882253597244	17.776801999594678
32	19.394041748294264	22.224549077889616	40.45936634465986	17.92204282915625
33	19.84259947307978	21.970546510842397	40.01486185232723	18.17199216375059
34	18.26454097142471	22.86833749915558	41.54360602580558	17.323515503614132
35	17.942984530162807	24.17212727149902	39.72843342565696	18.15645477268121
36	19.76558805647504	25.979868945484025	36.71553063568196	17.53901236235898
37	19.200837668040265	25.883266905357022	37.19583868134838	17.72005674525434
38	20.0533675606296	25.80693102749443	36.591231507126935	17.548469904749037
39	21.15990002026616	24.41329460244545	35.35499560899817	19.071809768290212
40	22.804161318651627	23.693170303316897	35.56508815780585	17.93758022022563
41	19.257582922380596	23.94447071539553	35.60697155981896	21.19097480240492
42	19.32851449030602	24.920624197797743	35.88124028913058	19.869621022765653
43	19.196108896845235	24.446396000810648	36.60136458825914	19.756130514084983
44	19.655475241505098	24.426129838546238	35.48064581503749	20.437749104911166
45	20.054718638113897	24.295075322569748	34.40721475376613	21.242991285550225
46	19.908802269810174	25.24420725528609	34.35317165439438	20.493818820509357
47	18.343579004255893	25.169222454907793	36.726339255556304	19.76085928528001
48	17.987570087144498	24.909140039181246	37.03100722826454	20.072282645409715
49	20.049989866918867	23.80193204080254	36.135242856177804	20.01283523610079
50	18.103087212051612	23.42092819023171	37.18435452273188	21.2916300749848
51	17.660609335945416	23.26150104708505	36.3730324934135	22.704857123556035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	172.0
1	285.0
2	398.0
3	6706.0
4	13014.0
5	8503.0
6	3992.0
7	3278.5
8	2565.0
9	2487.0
10	2409.0
11	2289.5
12	2170.0
13	2012.0
14	1854.0
15	1745.5
16	1637.0
17	1565.0
18	1493.0
19	1406.0
20	1319.0
21	1306.5
22	1294.0
23	1338.5
24	1383.0
25	1489.5
26	1777.5
27	1959.0
28	2137.5
29	2316.0
30	2494.0
31	2672.0
32	3067.0
33	3462.0
34	3671.5
35	3881.0
36	3985.5
37	4090.0
38	4346.0
39	4602.0
40	4837.0
41	5072.0
42	5585.0
43	6098.0
44	6969.0
45	7840.0
46	8840.0
47	9840.0
48	10149.0
49	10458.0
50	10058.0
51	9658.0
52	8691.0
53	7724.0
54	7132.0
55	6540.0
56	6278.5
57	6017.0
58	5601.0
59	5185.0
60	4909.0
61	4633.0
62	4071.5
63	3510.0
64	3060.0
65	2610.0
66	2179.5
67	1749.0
68	1429.5
69	1110.0
70	901.0
71	692.0
72	564.0
73	436.0
74	364.5
75	217.5
76	142.0
77	104.5
78	67.0
79	54.0
80	41.0
81	29.0
82	17.0
83	12.5
84	8.0
85	7.0
86	6.0
87	5.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	148030.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.13321623995137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.47032084742128	53.87894345740728
2	9.857298997926392	13.037897723434439
3	4.051196665883531	8.037559954063365
4	1.9806531354382668	5.239478484091062
5	1.0817491853682952	3.5769776396676347
6	0.5750942316924931	2.2819698709721004
7	0.3309600907075804	1.5321218671890833
8	0.19101708938986894	1.0106059582517057
9	0.10929854847441699	0.6505438086874282
>10	0.3299386089461373	3.566169019793285
>50	0.015322226421647241	0.7072890630277646
>100	0.004085927045772597	0.6478416537188407
>500	0.0	0.0
>1k	0.0030644452843294487	5.832601499696008
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4823	3.2581233533743164	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2729	1.8435452273187867	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1082	0.7309329190029048	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	496	0.33506721610484363	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	213	0.14388975207728164	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07566033912044856	0.0
2	0.0	0.0	0.0	0.2958859690603256	0.0
3	0.0	0.0	0.0	0.39991893535094236	0.0
4	0.0	0.0	0.0	0.5201648314530838	0.0
5	0.0	0.0	0.0	0.9511585489427818	0.0
6	0.0	0.0	0.0	1.4024184286968857	0.0
7	0.0	0.0	0.0	1.8394919948659056	0.0
8	0.0	0.0	0.0	2.759575761669932	0.0
9	0.0	0.0	0.0	3.287847058028778	0.0
10	0.0	0.0	0.0	4.169425116530433	0.0
11	0.0	0.0	0.0	5.169897993649935	0.0
12	0.0	0.0	0.0	5.96770924812538	0.0
13	0.0	0.0	0.0	6.3649260285077345	0.0
14	0.0	0.0	0.0	6.625008444234277	0.0
15	0.0	0.0	0.0	6.892521786124434	0.0
16	0.0	0.0	0.0	7.320813348645545	0.0
17	0.0	0.0	0.0	7.766668918462474	0.0
18	0.0	0.0	0.0	8.346281159224482	0.0
19	0.0	0.0	0.0	8.691481456461528	0.0
20	0.0	0.0	0.0	9.071809768290212	0.0
21	0.0	0.0	0.0	9.508207795717084	0.0
22	0.0	0.0	0.0	9.948659055596838	0.0
23	0.0	0.0	0.0	10.369519691954334	0.0
24	0.0	0.0	0.0	10.705262446801324	0.0
25	0.0	0.0	0.0	11.028845504289672	0.0
26	0.0	0.0	0.0	11.332162399513612	0.0
27	0.0	0.0	0.0	11.680064851719246	0.0
28	0.0	0.0	0.0	11.980004053232452	0.0
29	0.0	0.0	0.0	12.305613726947241	0.0
30	0.0	0.0	0.0	12.712963588461799	0.0
31	0.0	0.0	0.0	13.048706343308789	0.0
32	0.0	0.0	0.0	13.355400932243464	0.0
33	0.0	0.0	0.0	13.677632912247518	0.0
34	0.0	0.0	0.0	14.016077822063096	0.0
35	0.0	0.0	0.0	14.390326285212456	0.0
36	0.0	0.0	0.0	14.714584881442951	0.0
37	0.0	0.0	0.0	15.040194555157738	0.0
38	0.0	0.0	0.0	15.469161656420995	0.0
39	0.0	0.0	0.0	16.01972573127069	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCTC	25	3.874299E-5	45.0	5
CGGTTTA	90	0.0	45.0	1
TATGGGT	20	7.013362E-4	45.0	4
TCGGAAG	20	7.013362E-4	45.0	1
TACGAAT	50	2.1827873E-11	45.0	12
CGAATAT	50	2.1827873E-11	45.0	14
GGCGATT	25	3.874299E-5	45.0	8
TAGGAAC	20	7.013362E-4	45.0	6
GATTAGC	20	7.013362E-4	45.0	9
CTAGGAT	20	7.013362E-4	45.0	5
ACGAAAG	20	7.013362E-4	45.0	1
CGTTTTA	875	0.0	44.742855	1
CGTTATT	345	0.0	44.347824	1
CGTTTTT	1480	0.0	43.783783	1
GTTTTAT	925	0.0	43.2973	2
GCTACGA	55	6.002665E-11	40.909092	10
AGCTACG	55	6.002665E-11	40.909092	9
TACTGAC	50	1.0677468E-9	40.5	20
GTTATTT	385	0.0	40.324673	2
CGGTTTT	185	0.0	40.135136	1
>>END_MODULE