##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936166.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	72765
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45832474403903	31.0	31.0	34.0	30.0	34.0
2	31.618415446986877	31.0	31.0	34.0	30.0	34.0
3	30.683694083694085	31.0	30.0	34.0	26.0	34.0
4	34.970576513433656	37.0	35.0	37.0	32.0	37.0
5	31.566714766714767	35.0	35.0	37.0	0.0	37.0
6	33.59198790627362	35.0	35.0	37.0	17.0	37.0
7	21.329114271971413	32.0	0.0	37.0	0.0	37.0
8	28.34476740191026	35.0	17.0	37.0	17.0	37.0
9	34.71156462585034	37.0	32.0	39.0	28.0	39.0
10	36.22179619322476	37.0	35.0	39.0	32.0	39.0
11	36.67084449941593	38.0	35.0	39.0	33.0	39.0
12	37.05512265512265	39.0	37.0	39.0	34.0	39.0
13	36.85170068027211	39.0	37.0	39.0	33.0	39.0
14	38.164447193018624	40.0	38.0	41.0	33.0	41.0
15	38.30988799560228	40.0	38.0	41.0	33.0	41.0
16	38.38591355734213	40.0	38.0	41.0	34.0	41.0
17	38.355692984264415	40.0	38.0	41.0	34.0	41.0
18	38.125788497217066	40.0	37.0	41.0	33.0	41.0
19	37.89683226826084	39.0	37.0	41.0	33.0	41.0
20	37.664770150484436	39.0	35.0	41.0	33.0	41.0
21	37.53766233766234	39.0	35.0	40.0	33.0	41.0
22	37.41719233147805	39.0	35.0	40.0	33.0	41.0
23	37.31171579743008	39.0	35.0	40.0	33.0	41.0
24	37.0664467807325	38.0	35.0	40.0	33.0	41.0
25	36.929031814746104	38.0	35.0	40.0	33.0	41.0
26	36.779880437023294	38.0	35.0	40.0	32.0	41.0
27	36.70046038617467	38.0	35.0	40.0	32.0	41.0
28	36.58111729540301	38.0	35.0	40.0	32.0	41.0
29	36.541922627636914	38.0	35.0	40.0	32.0	41.0
30	36.252965024393596	38.0	35.0	40.0	31.0	41.0
31	36.05809111523397	38.0	35.0	40.0	30.0	41.0
32	35.76852882567168	38.0	34.0	40.0	30.0	41.0
33	35.28090428090428	38.0	34.0	40.0	25.0	41.0
34	34.73459767745482	38.0	33.0	40.0	23.0	41.0
35	34.128372156943584	38.0	33.0	40.0	20.0	41.0
36	33.65060125060125	37.0	33.0	40.0	16.0	41.0
37	33.40937263794407	37.0	33.0	40.0	15.0	41.0
38	33.127451384594245	37.0	33.0	40.0	15.0	41.0
39	32.973380059094346	37.0	32.0	40.0	12.0	41.0
40	32.78576238576239	37.0	32.0	40.0	10.0	41.0
41	32.65567236995808	37.0	31.0	40.0	10.0	41.0
42	32.514931629217344	37.0	31.0	40.0	10.0	41.0
43	32.37090634233491	37.0	31.0	40.0	10.0	41.0
44	32.15915618772762	36.0	30.0	40.0	10.0	41.0
45	32.017288531574245	36.0	30.0	40.0	10.0	41.0
46	31.863711949426236	36.0	30.0	40.0	10.0	41.0
47	31.762509448223735	35.0	29.0	40.0	10.0	41.0
48	31.636844636844636	35.0	29.0	40.0	9.0	41.0
49	31.507015735587164	35.0	28.0	40.0	8.0	41.0
50	31.278086992372707	35.0	28.0	40.0	8.0	41.0
51	29.498866213151928	34.0	23.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	2.0
12	1.0
13	0.0
14	4.0
15	4.0
16	4.0
17	14.0
18	38.0
19	67.0
20	113.0
21	220.0
22	422.0
23	787.0
24	1190.0
25	1807.0
26	2393.0
27	2440.0
28	2357.0
29	2146.0
30	2155.0
31	2476.0
32	3065.0
33	4117.0
34	5302.0
35	6761.0
36	7449.0
37	11066.0
38	10051.0
39	6308.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.72369958084244	17.516663230948947	18.422318422318423	27.337318765890195
2	42.49845392702535	21.859410430839002	21.280835566549854	14.36130007558579
3	27.275475846904417	21.712361712361712	37.91383219954649	13.098330241187384
4	22.866762866762866	21.669758812615957	40.27898027898028	15.184498041640898
5	19.018759018759017	32.26688655260084	34.32694289837147	14.387411530268674
6	20.630797773654916	29.154126296983442	38.56112141826428	11.653954511097368
7	38.214801071943924	41.209372637944064	17.45894317322889	3.116883116883117
8	64.04040404040404	6.40830069401498	25.278636707208136	4.272658558372844
9	58.651824366110084	7.535216106644678	27.329073043358758	6.483886483886484
10	32.56510685082114	21.71098742527314	33.37593623307909	12.347969490826634
11	23.05916305916306	22.38851095993953	38.98027898027898	15.572047000618431
12	21.11317254174397	20.4397718683433	42.587782587782584	15.859273002130145
13	19.443413729128014	20.030234315948604	44.785267642410496	15.741084312512884
14	15.87713873428159	23.096268810554523	43.2543118257404	17.772280629423488
15	15.411255411255413	21.92262763691335	46.18429189857761	16.481825053253623
16	18.47041847041847	22.605648319934033	41.99821342678485	16.92571978286264
17	18.169449598021025	22.422868137153852	41.31794131794132	18.089740946883804
18	19.611076753933897	21.59417302274445	42.822785679928536	15.971964543393113
19	18.64632721775579	23.328523328523328	40.094825809111526	17.93032364460936
20	18.967910396481823	24.125609839895553	41.56806156806157	15.33841819556105
21	19.15343915343915	24.734419020133306	41.25884697313269	14.853294853294855
22	18.051260908403766	21.752216037930324	41.26571840857555	18.93080464509036
23	16.747062461348175	23.388991960420533	42.24970796399368	17.614237614237616
24	17.546897546897547	21.99546485260771	42.39675668247097	18.060880918023773
25	17.487803202088916	24.13935271078128	40.41778327492614	17.95506081220367
26	17.805263519549232	22.839277124991412	40.185528756957325	19.169930598502027
27	18.021026592455165	22.71971414828558	41.60654160654161	17.652717652717655
28	16.42960214388786	23.013811585240155	41.84841613413042	18.708170136741565
29	19.26475640761355	22.66611695183124	39.94365422936851	18.125472411186696
30	18.38246409674981	22.534185391328247	41.929499072356215	17.153851439565727
31	20.248745963031677	22.39400810829382	39.5808424379853	17.776403490689205
32	20.732495018209303	23.672095100666528	38.93630179344465	16.659108087679517
33	19.33347076204219	23.765546622689477	38.42369270940699	18.477289905861337
34	19.437916580773724	22.983577269291555	39.49563663849378	18.08286951144094
35	18.633958633958635	25.38857967429396	37.98117226688655	17.996289424860855
36	20.204768776197348	27.324950182093037	35.9527245241531	16.517556517556518
37	19.909297052154194	26.95114409400124	35.387892530749674	17.751666323094895
38	19.91479420050849	28.548065690922837	33.77035662749949	17.766783481069197
39	19.835085549371264	26.17879475022332	34.35442864014293	19.631691060262487
40	20.780595066309353	26.09496323782038	34.761217618360476	18.36322407750979
41	18.283515426372567	27.077578506149933	33.7744794887652	20.86442657871229
42	19.494262351405208	26.383563526420666	35.33292104720676	18.78925307496736
43	20.698137840994985	25.416065416065415	34.611420325706035	19.27437641723356
44	20.674774960489245	25.509516938088368	33.98062255205112	19.835085549371264
45	19.770494056208342	24.8155019583591	33.665910808767954	21.748093176664604
46	20.259740259740262	26.342334913763487	33.40479626193912	19.993128564557136
47	18.315124029409745	25.686799972514258	36.160241874527586	19.837834123548408
48	18.781007352435925	26.27636913351199	34.62516319659177	20.317460317460316
49	19.79110836253693	24.595616024187454	35.969215969215966	19.644059644059645
50	17.98529512815227	24.958427815570673	35.7452071737786	21.311069882498455
51	18.02789802789803	24.106369820655534	34.51109736824022	23.354634783206212
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	132.0
1	188.5
2	245.0
3	2894.0
4	5543.0
5	3629.5
6	1716.0
7	1412.5
8	1109.0
9	1061.5
10	1014.0
11	978.5
12	943.0
13	908.0
14	873.0
15	807.5
16	742.0
17	724.0
18	706.0
19	678.5
20	651.0
21	658.5
22	666.0
23	726.5
24	787.0
25	856.5
26	1006.5
27	1087.0
28	1165.5
29	1244.0
30	1413.0
31	1582.0
32	1740.0
33	1898.0
34	2061.5
35	2225.0
36	2260.5
37	2296.0
38	2408.0
39	2520.0
40	2687.5
41	2855.0
42	3103.5
43	3352.0
44	3757.0
45	4162.0
46	4881.0
47	5600.0
48	5457.0
49	5314.0
50	5081.0
51	4848.0
52	4325.5
53	3803.0
54	3374.5
55	2946.0
56	2750.5
57	2555.0
58	2353.5
59	2152.0
60	2036.5
61	1921.0
62	1704.0
63	1487.0
64	1261.5
65	1036.0
66	855.0
67	674.0
68	565.5
69	457.0
70	375.5
71	294.0
72	237.5
73	181.0
74	156.5
75	88.5
76	45.0
77	36.0
78	27.0
79	20.0
80	13.0
81	8.0
82	3.0
83	2.0
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	72765.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.30770287913145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.08840392084335	63.970315398886825
2	8.78305899759571	13.05297876726448
3	2.922137969299057	6.514120799835085
4	1.0800813759940817	3.2103346389060676
5	0.44201960421675607	1.6422730708444995
6	0.25152579988903273	1.1214182642754071
7	0.12206399112261881	0.6349206349206349
8	0.06473090438320696	0.3848003848003848
9	0.03144072498612909	0.21026592455163884
>10	0.18864434991677456	2.398130969559541
>50	0.012946180876641391	0.7627293341579056
>100	0.009247272054743851	1.4993472136329278
>500	0.0	0.0
>1k	0.00369890882189754	4.598364598364598
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2097	2.8818800247371676	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1249	1.7164845736274308	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	478	0.6569092283377997	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	221	0.30371744657458943	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG	161	0.22126022126022124	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	118	0.16216587645159075	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG	113	0.15529444100872672	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	97	0.1333058475915619	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC	94	0.12918298632584346	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86	0.11818868961726106	No Hit
GAATCTATCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	78	0.10719439290867862	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	76	0.10444581873153302	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07833436404864977	0.0
2	0.0	0.0	0.0	0.4329004329004329	0.0
3	0.0	0.0	0.0	0.5689548546691404	0.0
4	0.0	0.0	0.0	0.732495018209304	0.0
5	0.0	0.0	0.0	1.3358070500927643	0.0
6	0.0	0.0	0.0	1.7728303442589157	0.0
7	0.0	0.0	0.0	2.329416615130901	0.0
8	0.0	0.0	0.0	3.4178519892805608	0.0
9	0.0	0.0	0.0	3.8796124510410226	0.0
10	0.0	0.0	0.0	5.230536659108088	0.0
11	0.0	0.0	0.0	6.303854875283447	0.0
12	0.0	0.0	0.0	7.355184498041641	0.0
13	0.0	0.0	0.0	7.720744863602007	0.0
14	0.0	0.0	0.0	7.925513639799354	0.0
15	0.0	0.0	0.0	8.30619116333402	0.0
16	0.0	0.0	0.0	8.843537414965986	0.0
17	0.0	0.0	0.0	9.428983714698001	0.0
18	0.0	0.0	0.0	10.048787191644335	0.0
19	0.0	0.0	0.0	10.443207586064728	0.0
20	0.0	0.0	0.0	10.898096612382327	0.0
21	0.0	0.0	0.0	11.376348519205662	0.0
22	0.0	0.0	0.0	11.879337593623308	0.0
23	0.0	0.0	0.0	12.367209510066653	0.0
24	0.0	0.0	0.0	12.816601388029959	0.0
25	0.0	0.0	0.0	13.239881811310383	0.0
26	0.0	0.0	0.0	13.580705009276437	0.0
27	0.0	0.0	0.0	13.953136810279668	0.0
28	0.0	0.0	0.0	14.302205730777159	0.0
29	0.0	0.0	0.0	14.681508967223253	0.0
30	0.0	0.0	0.0	15.161135161135162	0.0
31	0.0	0.0	0.0	15.529444100872672	0.0
32	0.0	0.0	0.0	15.879887308458738	0.0
33	0.0	0.0	0.0	16.29904487047344	0.0
34	0.0	0.0	0.0	16.682470968185253	0.0
35	0.0	0.0	0.0	17.10300281728853	0.0
36	0.0	0.0	0.0	17.476808905380334	0.0
37	0.0013742870885728028	0.0	0.0	17.85198928056071	0.0
38	0.0013742870885728028	0.0	0.0	18.271146842575416	0.0
39	0.0013742870885728028	0.0	0.0	18.827733113447398	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACT	40	6.664777E-9	45.000004	3
CTGTCAC	40	6.664777E-9	45.000004	2
ATAGGAT	20	6.989753E-4	45.000004	5
GTCACTT	40	6.664777E-9	45.000004	4
TCACTTA	40	6.664777E-9	45.000004	5
AAGGGCG	20	6.989753E-4	45.000004	5
GTAAGGC	20	6.989753E-4	45.000004	4
ACACGCG	25	3.8547674E-5	45.0	36
CGGTTTA	25	3.8547674E-5	45.0	1
CACGCGG	25	3.8547674E-5	45.0	37
CGTTATT	180	0.0	45.0	1
CGTTTTT	665	0.0	43.984962	1
CGTTTTA	415	0.0	42.831326	1
GTTTTAT	450	0.0	41.0	2
CTTAATC	50	1.0513759E-9	40.5	11
ATGGGAT	50	1.0513759E-9	40.5	5
TGAAGGG	45	1.8859282E-8	40.0	3
GACCCGC	45	1.8859282E-8	40.0	31
ACGGGGG	45	1.8859282E-8	40.0	5
ATCAACG	45	1.8859282E-8	40.0	15
>>END_MODULE