##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936165.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86465
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.621951078471056	31.0	31.0	34.0	30.0	34.0
2	31.78730122014688	31.0	31.0	34.0	30.0	34.0
3	30.75642167350951	31.0	31.0	34.0	26.0	34.0
4	35.048667090730355	37.0	35.0	37.0	32.0	37.0
5	31.542635748568784	35.0	35.0	37.0	0.0	37.0
6	33.65528248424218	37.0	35.0	37.0	17.0	37.0
7	21.356988376799862	32.0	0.0	37.0	0.0	37.0
8	28.420170010987103	35.0	17.0	37.0	17.0	37.0
9	34.90700283351645	37.0	32.0	39.0	32.0	39.0
10	36.36396229688313	37.0	35.0	39.0	32.0	39.0
11	36.8339675013011	39.0	37.0	39.0	33.0	39.0
12	37.28568785057538	39.0	37.0	39.0	34.0	39.0
13	37.16448273868039	39.0	37.0	39.0	34.0	39.0
14	38.66662811542243	40.0	38.0	41.0	35.0	41.0
15	38.782200890533744	40.0	38.0	41.0	35.0	41.0
16	38.775435147169375	40.0	38.0	41.0	35.0	41.0
17	38.72742728271555	40.0	38.0	41.0	35.0	41.0
18	38.493401954548084	40.0	38.0	41.0	35.0	41.0
19	38.278216619441395	40.0	37.0	41.0	34.0	41.0
20	38.04674723876713	40.0	36.0	41.0	34.0	41.0
21	37.92051118949864	40.0	36.0	41.0	33.0	41.0
22	37.77921702422946	39.0	35.0	41.0	34.0	41.0
23	37.622193951309775	39.0	35.0	41.0	33.0	41.0
24	37.396773260856996	39.0	35.0	41.0	33.0	41.0
25	37.25638119470306	39.0	35.0	41.0	33.0	41.0
26	37.141479211241545	39.0	35.0	40.0	33.0	41.0
27	37.10335974093564	39.0	35.0	40.0	33.0	41.0
28	37.02214768981669	39.0	35.0	40.0	33.0	41.0
29	37.00959925981611	39.0	35.0	40.0	33.0	41.0
30	36.82972300931012	39.0	35.0	40.0	33.0	41.0
31	36.642086393338346	39.0	35.0	40.0	32.0	41.0
32	36.41316139478402	39.0	35.0	40.0	31.0	41.0
33	35.90704909500954	38.0	35.0	40.0	29.0	41.0
34	35.25412594691494	38.0	34.0	40.0	24.0	41.0
35	34.62492337940207	38.0	34.0	40.0	20.0	41.0
36	34.14812930087319	38.0	33.0	40.0	16.0	41.0
37	33.90606603828139	38.0	33.0	40.0	15.0	41.0
38	33.66163187416874	38.0	33.0	40.0	12.0	41.0
39	33.51257734343376	38.0	33.0	40.0	10.0	41.0
40	33.41432949748453	38.0	33.0	40.0	10.0	41.0
41	33.31325970045683	38.0	33.0	40.0	10.0	41.0
42	33.15875787891054	38.0	33.0	40.0	10.0	41.0
43	33.03617648759614	38.0	32.0	40.0	10.0	41.0
44	32.84451512172556	38.0	32.0	40.0	10.0	41.0
45	32.78978777540045	38.0	32.0	40.0	10.0	41.0
46	32.64971953969815	38.0	31.0	40.0	10.0	41.0
47	32.59680795697681	38.0	31.0	40.0	10.0	41.0
48	32.50080379344244	38.0	31.0	40.0	9.0	41.0
49	32.42796507257272	38.0	31.0	40.0	8.0	41.0
50	32.2400508876424	37.0	30.0	40.0	8.0	41.0
51	30.604603018562425	35.0	26.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	1.0
12	1.0
13	1.0
14	2.0
15	3.0
16	6.0
17	11.0
18	36.0
19	78.0
20	130.0
21	221.0
22	429.0
23	762.0
24	1431.0
25	2260.0
26	2937.0
27	2870.0
28	2485.0
29	2152.0
30	2124.0
31	2281.0
32	2846.0
33	3748.0
34	4997.0
35	6771.0
36	8482.0
37	14221.0
38	15050.0
39	10124.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.41698953333719	17.40935638697739	20.8789683687041	28.29468571098132
2	43.301914069276584	22.09564563696293	21.44798473370728	13.154455560053202
3	27.160122592956686	20.79107153183369	39.284103394437054	12.764702480772566
4	23.318105591857975	21.615682646157406	40.56554675302145	14.500665008963164
5	18.794888105013587	33.322153472503324	34.10859885502804	13.774359567455038
6	19.444862082923724	31.129358700052045	38.2420632625918	11.18371595443243
7	38.58555484878274	41.62146533279362	17.164170473602034	2.628809344821604
8	66.41878216619442	6.345920314578153	24.1045509743827	3.130746544844735
9	60.72977505348985	8.557219684265311	25.653154455560053	5.0598508066847865
10	29.150523333140576	26.813161394784018	33.00294917018447	11.03336610189094
11	21.08136240097149	24.368241484993927	39.91904238709304	14.631353726941537
12	19.599838084774184	21.771815185335104	43.14925114208061	15.479095587810097
13	17.716995316023826	22.14653327936159	44.748742265656624	15.387729138957958
14	16.04579887815879	25.413751228820907	42.40444110333661	16.136008789683686
15	16.337246284623834	25.07719886659342	44.852830625108425	13.73272422367432
16	17.925172034927428	23.922974614005668	43.23252182964205	14.919331521424853
17	17.4452090441219	23.911409240733246	41.845833574278615	16.797548140866247
18	17.453304805412596	24.58335742786098	42.19395130977852	15.769386456947899
19	18.472214190713004	25.404498930202973	40.2382466894119	15.885040189672123
20	19.06320476493379	25.2437402417163	41.65500491528364	14.03805007806627
21	18.73243508934251	25.30734979471462	41.36587058347308	14.594344532469785
22	18.067426126178223	23.27878332273174	41.44914127103452	17.204649280055513
23	17.075117099404384	25.49123922974614	41.638813392702254	15.794830278147225
24	17.46602671601226	24.07563754120164	42.35702307291968	16.10131266986642
25	17.26478922107211	25.589544902561727	41.15422425258775	15.991441623778407
26	16.14757416295611	26.150465506274216	40.88475105534031	16.817209275429366
27	17.051986352859537	25.889088070317467	41.569421153067715	15.489504423755276
28	15.64679350026022	26.44769559937547	42.101428323599144	15.804082576765166
29	16.725842826577228	25.443821199329207	41.35314867287342	16.477187301220148
30	16.13253917770196	26.13658705834731	41.80766784247962	15.923205921471114
31	17.320303012779735	25.898340368935408	40.4464234083155	16.334933209969353
32	17.20233620540103	26.625802347770772	39.84618053547678	16.325680911351416
33	17.271728445035563	25.64274561961487	39.526976233157924	17.55854970219164
34	16.710807841323078	26.875614410455096	39.278320707800845	17.135257040420978
35	17.490313999884346	26.075290580003468	38.030416931706476	18.403978488405713
36	17.727404151969004	28.8717978372752	36.134852252356445	17.265945758399354
37	16.87156652980975	28.442722488868327	37.013820621060546	17.67189036026138
38	17.48106170126641	28.941190076909734	35.357659168449665	18.220089053374195
39	18.479153414676457	27.03406002428728	35.13676053894639	19.350026022089864
40	18.40166541375123	27.647024807725668	36.25975828369861	17.691551494824495
41	16.547736078181924	27.704851674087784	36.46909153992945	19.278320707800845
42	17.50303591048401	28.69253455155265	35.42936448273868	18.375065055224656
43	18.530041057075117	27.914184930318626	34.771294743537844	18.78447926906841
44	17.985311975944025	28.12351818654947	34.8083039380096	19.082865899496905
45	18.446770369513676	27.606546001272193	34.46249927716417	19.48418435204996
46	18.37159544324293	29.121609899959523	34.02995431677557	18.476840340021976
47	17.655698837679985	28.1489620077488	35.887353264326606	18.30798589024461
48	17.2809807436535	28.32822529347135	34.82102584860927	19.569768114265887
49	17.224310414618632	27.909558781009657	35.753194934366505	19.112935870005206
50	16.957150292025673	26.764586827039842	35.90817093621696	20.370091944717515
51	16.726999363904472	26.311224194760886	35.27207540623374	21.68970103510091
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89.0
1	145.5
2	202.0
3	3033.0
4	5864.0
5	4081.0
6	2298.0
7	1962.5
8	1627.0
9	1525.0
10	1423.0
11	1344.0
12	1265.0
13	1198.0
14	1131.0
15	1090.5
16	1050.0
17	969.5
18	889.0
19	901.5
20	914.0
21	910.0
22	906.0
23	906.5
24	907.0
25	1066.5
26	1407.5
27	1589.0
28	1780.5
29	1972.0
30	2203.5
31	2435.0
32	2789.5
33	3144.0
34	3434.5
35	3725.0
36	3855.0
37	3985.0
38	4193.0
39	4401.0
40	4551.5
41	4702.0
42	4842.0
43	4982.0
44	5187.5
45	5393.0
46	5840.0
47	6287.0
48	5780.5
49	5274.0
50	4923.0
51	4572.0
52	3909.0
53	3246.0
54	2876.0
55	2506.0
56	2304.0
57	2102.0
58	1998.5
59	1895.0
60	1759.0
61	1623.0
62	1390.5
63	1158.0
64	916.5
65	675.0
66	544.5
67	414.0
68	329.0
69	244.0
70	198.5
71	153.0
72	125.5
73	98.0
74	70.5
75	34.5
76	26.0
77	20.5
78	15.0
79	11.0
80	7.0
81	4.5
82	2.0
83	2.0
84	2.0
85	2.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	86465.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.04915283640779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.71859727909127	57.943676632163296
2	9.899616959450535	13.869195628288903
3	3.7197860256240918	7.817035794830278
4	1.6576409985470875	4.644653906204823
5	0.8189142781666887	2.8682125715607474
6	0.4424778761061947	1.8597120222055168
7	0.22123893805309736	1.084832012953218
8	0.14033813234711398	0.786445382524721
9	0.0759476951525558	0.47880645347828604
>10	0.28067626469422796	3.0046839761753312
>50	0.01320829480914014	0.6997050829815532
>100	0.008255184255712587	1.250216850748858
>500	0.0	0.0
>1k	0.003302073702285035	3.692823685884462
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2032	2.350083848956225	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1161	1.3427398369282368	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.5215983345862487	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	254	0.2937604811195281	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCG	143	0.16538483779563984	No Hit
GAATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTC	125	0.1445671659052796	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGC	108	0.12490603134216156	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93	0.10755797143352801	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCG	92	0.10640143410628577	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03816573179899381	0.0
2	0.0	0.0	0.0	0.22783785346672064	0.0
3	0.0	0.0	0.0	0.2995431677557393	0.0
4	0.0	0.0	0.0	0.41404036315272075	0.0
5	0.0	0.0	0.0	0.8211415023419881	0.0
6	0.0	0.0	0.0	1.104493147516336	0.0
7	0.0	0.0	0.0	1.4132886138900134	0.0
8	0.0	0.0	0.0	2.072514890418088	0.0
9	0.0	0.0	0.0	2.3870930434279765	0.0
10	0.0	0.0	0.0	3.1596599780257906	0.0
11	0.0	0.0	0.0	3.732145955010698	0.0
12	0.0	0.0	0.0	4.414502978083617	0.0
13	0.0	0.0	0.0	4.626149308968947	0.0
14	0.0	0.0	0.0	4.741803041693171	0.0
15	0.0	0.0	0.0	4.912970566125022	0.0
16	0.0	0.0	0.0	5.192852599317643	0.0
17	0.0	0.0	0.0	5.544439946799283	0.0
18	0.0	0.0	0.0	5.9411322500433705	0.0
19	0.0	0.0	0.0	6.189787775400451	0.0
20	0.0	0.0	0.0	6.41646909153993	0.0
21	0.0	0.0	0.0	6.713699184641184	0.0
22	0.0	0.0	0.0	7.00630312843347	0.0
23	0.0	0.0	0.0	7.338229341351992	0.0
24	0.0	0.0	0.0	7.63198982247152	0.0
25	0.0	0.0	0.0	7.880645347828601	0.0
26	0.0	0.0	0.0	8.111952813277048	0.0
27	0.0	0.0	0.0	8.343260278725495	0.0
28	0.0	0.0	0.0	8.57225466951946	0.0
29	0.0	0.0	0.0	8.845197478748627	0.0
30	0.0	0.0	0.0	9.141271034522639	0.0
31	0.0	0.0	0.0	9.42577921702423	0.0
32	0.0	0.0	0.0	9.719539698143757	0.0
33	0.0	0.0	0.0	10.04221361244434	0.0
34	0.0	0.0	0.0	10.330191406927659	0.0
35	0.0	0.0	0.0	10.643613022610305	0.0
36	0.0	0.0	0.0	10.937373503729832	0.0
37	0.0	0.0	0.0	11.236916671485572	0.0
38	0.0	0.0	0.0	11.580408257676517	0.0
39	0.0	0.0	0.0	11.905395246631585	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGATT	20	6.997106E-4	45.0	6
CTGTCAC	20	6.997106E-4	45.0	2
ATCTATC	20	6.997106E-4	45.0	3
CTATCTC	35	1.1945849E-7	45.0	5
CGGTTTT	70	0.0	45.0	1
CGGTTTA	20	6.997106E-4	45.0	1
AATGGGT	20	6.997106E-4	45.0	4
TACGGGA	25	3.8608472E-5	45.0	4
TGCGGGA	20	6.997106E-4	45.0	4
CTGCGGG	25	3.8608472E-5	45.0	3
GTCACTT	20	6.997106E-4	45.0	4
GACGGGA	25	3.8608472E-5	45.0	4
CGCAGGA	20	6.997106E-4	45.0	4
AATTATC	20	6.997106E-4	45.0	45
CTACAGG	20	6.997106E-4	45.0	3
ATGCGGG	25	3.8608472E-5	45.0	3
TTACGGG	20	6.997106E-4	45.0	3
GGCATAT	20	6.997106E-4	45.0	8
TAGGATG	25	3.8608472E-5	45.0	6
GGCGATC	20	6.997106E-4	45.0	8
>>END_MODULE