FastQCFastQC Report
Sat 14 Jan 2017
SRR2936162.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936162.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151544
Sequences flagged as poor quality0
Sequence length51
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51973.4293670485139627No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29701.9598268489679567No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.750937021591089No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.38206725439476324No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.15375072586179592No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCG1540.10162065142796811No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG207.013888E-445.0000042
GGAAGTA207.013888E-445.0000048
CCTCACC302.1529977E-645.00000430
GGGTTGC207.013888E-445.0000048
GCTGTAT207.013888E-445.0000042
ACAACGA253.8747366E-544.99999613
CGTATGG253.8747366E-544.9999962
CAACGAG253.8747366E-544.99999614
CGGTTTA1000.044.9999961
CGTTATT5650.044.9999961
ACTACGG253.8747366E-544.9999962
GGTATGC253.8747366E-544.9999968
CGAGCGA253.8747366E-544.99999617
CGTTTTA11100.044.79731
CGTTTTT21400.044.5794371
ATAGGAT603.6379788E-1241.2500045
GTTTTAT12000.041.252
GTTATTT6300.040.7142872
GATGCCA1550.040.645169
AGGCGAT451.9093932E-840.07
Produced by FastQC (version 0.11.5)