##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936162.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151544
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.416789843213852	31.0	31.0	34.0	28.0	34.0
2	31.574097291875628	31.0	31.0	34.0	30.0	34.0
3	30.128774481338752	31.0	30.0	34.0	25.0	34.0
4	34.68360344190466	35.0	35.0	37.0	32.0	37.0
5	31.37812780446603	35.0	35.0	37.0	0.0	37.0
6	33.542634482394554	35.0	35.0	37.0	17.0	37.0
7	21.556056326875364	32.0	0.0	37.0	0.0	37.0
8	28.5667198965317	35.0	17.0	37.0	17.0	37.0
9	35.02716702739799	37.0	32.0	39.0	32.0	39.0
10	36.460374544686694	37.0	35.0	39.0	32.0	39.0
11	36.899844269651055	39.0	37.0	39.0	33.0	39.0
12	37.307230903236025	39.0	37.0	39.0	34.0	39.0
13	37.184335902444175	39.0	37.0	39.0	34.0	39.0
14	38.69786332682257	40.0	38.0	41.0	35.0	41.0
15	38.79978752045611	40.0	38.0	41.0	35.0	41.0
16	38.82289632054057	40.0	38.0	41.0	35.0	41.0
17	38.78219527002059	40.0	38.0	41.0	35.0	41.0
18	38.48672332787837	40.0	38.0	41.0	35.0	41.0
19	38.18024468141266	40.0	37.0	41.0	35.0	41.0
20	37.83001636488412	39.0	35.0	41.0	34.0	41.0
21	37.6857942247796	39.0	35.0	41.0	33.0	41.0
22	37.48024336166394	39.0	35.0	40.0	33.0	41.0
23	37.281739956712244	38.0	35.0	40.0	33.0	41.0
24	36.979675869714406	38.0	35.0	40.0	33.0	41.0
25	36.83660850973975	38.0	35.0	40.0	33.0	41.0
26	36.718015889774584	38.0	35.0	40.0	33.0	41.0
27	36.636310246529064	38.0	35.0	40.0	33.0	41.0
28	36.54695665945204	38.0	35.0	40.0	33.0	41.0
29	36.49703716412395	38.0	35.0	40.0	33.0	41.0
30	36.32077152510162	38.0	35.0	40.0	32.0	41.0
31	36.073351633848915	38.0	35.0	40.0	31.0	41.0
32	35.684137940136196	38.0	34.0	40.0	30.0	41.0
33	35.00783270865227	38.0	33.0	40.0	24.0	41.0
34	34.15126959826849	38.0	33.0	40.0	20.0	41.0
35	33.290159953544844	38.0	33.0	40.0	15.0	41.0
36	32.63993955550863	38.0	31.0	40.0	10.0	41.0
37	32.237713139418254	38.0	31.0	40.0	8.0	41.0
38	31.95625032993718	37.0	30.0	40.0	8.0	41.0
39	31.79883070263422	37.0	30.0	40.0	8.0	41.0
40	31.638698991711976	37.0	29.0	40.0	7.0	41.0
41	31.462110014253287	37.0	27.0	40.0	7.0	41.0
42	31.278242622604655	37.0	26.0	40.0	7.0	41.0
43	31.12442590930687	37.0	25.0	40.0	7.0	41.0
44	30.955702634218444	37.0	24.0	40.0	7.0	41.0
45	30.867345457424907	36.0	24.0	40.0	7.0	41.0
46	30.719705168135988	36.0	23.0	40.0	7.0	41.0
47	30.685206936599272	36.0	23.0	40.0	7.0	41.0
48	30.54603283534815	36.0	23.0	40.0	7.0	41.0
49	30.432217705748826	36.0	23.0	40.0	7.0	41.0
50	30.25607744285488	35.0	23.0	40.0	7.0	41.0
51	28.869556036530646	34.0	20.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	5.0
11	7.0
12	2.0
13	3.0
14	1.0
15	3.0
16	11.0
17	29.0
18	74.0
19	142.0
20	286.0
21	585.0
22	1048.0
23	2042.0
24	3533.0
25	5455.0
26	6992.0
27	6633.0
28	5634.0
29	4672.0
30	4191.0
31	4205.0
32	5152.0
33	6861.0
34	8534.0
35	11472.0
36	13671.0
37	21607.0
38	23141.0
39	15548.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.89288919389748	16.844612785725598	20.679802565591512	34.58269545478541
2	48.84785936757641	19.98759436203347	19.73024336166394	11.434302908726178
3	24.126986221823365	18.712717098664413	45.415852821622764	11.744443857889458
4	20.064139787784406	19.983635115873938	47.52481127593306	12.427413820408596
5	16.811619067729506	31.280024283376445	40.0312780446603	11.877078604233754
6	17.965079448872935	27.1148973235496	44.90511006704324	10.014913160534235
7	35.36464657129282	40.136857942247794	22.311011983318377	2.187483503141002
8	61.60784986538563	4.712822678562001	31.25098981153988	2.428337644512485
9	57.16623554875152	6.734017843002691	32.40379031832339	3.6959562899223988
10	26.325687589083042	27.3352953597635	37.812120572243046	8.526896478910416
11	18.152483767090747	21.991632793116192	47.739270442907674	12.116612996885392
12	17.866098294884655	19.524362561368317	49.51763184289712	13.091907300849916
13	15.51562582484295	20.72797339386581	50.22039803621391	13.536002745077338
14	13.869239296837883	22.793380140421263	48.760755952066724	14.576624610674127
15	13.446259832127962	22.88312305337064	50.915245737211634	12.755371377289764
16	15.442379770891623	22.19289447289236	48.533099297893685	13.831626458322335
17	15.394208942617327	22.102491685583065	47.88114343029088	14.622155941508735
18	15.038536662619437	22.579580847806575	48.60634535184501	13.775537137728977
19	15.52288444280209	23.25793168980626	47.60927519400306	13.609908673388588
20	15.968959510109276	22.791400517341497	48.044132397191575	13.19550757535765
21	15.50110858892467	22.92205564060603	48.23747558464868	13.33936018582062
22	15.497149342765137	21.23277727920604	48.10022171778493	15.169851660243888
23	14.610938077390065	22.65414665047775	48.87557409069313	13.859341181439053
24	14.209074592197645	22.359842685952593	49.040542680673596	14.39054004117616
25	13.778836509528587	23.876233965053054	47.815155994298685	14.529773531119675
26	14.0869978356121	23.757456580267117	47.46476270918017	14.690782874940611
27	14.075120097133507	23.744259093068678	48.30412289500079	13.876497914797023
28	13.551839729715462	23.453914374703054	48.82410389061923	14.170142004962255
29	14.033548012458427	22.405374016787206	48.517922187615476	15.04315578313889
30	13.598030934909994	23.011798553555405	48.98841260623977	14.401757905294833
31	15.60338911471256	22.377659293670487	47.42055112706541	14.59840046455155
32	14.639972549226627	23.58522937232751	47.034524626511114	14.740273451934751
33	14.928337644512485	23.42092065670696	46.61022541308135	15.040516285699205
34	14.070500976614053	23.825423639339068	46.73692129018635	15.36715409386053
35	15.180409650002641	23.87491421633321	45.03642506466769	15.908251068996462
36	16.48498125956818	25.5219606186982	43.07131922082036	14.921738900913267
37	16.144486089848492	26.370559045557723	42.17454996568653	15.310404898907247
38	16.164282320646148	27.21255872881803	40.22396135775748	16.399197592778336
39	16.95877104999208	26.594916327931163	40.1672121628042	16.279100459272556
40	16.847252283165286	26.55862323813546	40.57633426595576	16.017790212743492
41	16.624874623871616	26.441165602069365	39.66306815182389	17.270891622235126
42	16.94095444227419	26.685319115240457	39.207094969117875	17.16663147336747
43	18.024468141265903	26.959166974608035	37.99358602122156	17.022778862904502
44	16.995724014147704	27.368289077759595	37.97115029298422	17.664836615108484
45	16.714617536820988	27.5886871139735	37.44391067940664	18.25278466979887
46	17.03663622446286	27.657314047405375	37.8952647415932	17.410784986538562
47	16.51731510320435	26.792878635907723	39.217652958876634	17.472153302011296
48	16.77400622921396	26.70313572295835	38.551179855355535	17.971678192472154
49	16.468484400570134	26.55532386633585	39.12395079976773	17.852240933326293
50	15.803990920128808	26.648366151084836	38.83690545320171	18.71073747558465
51	16.145145964208414	25.170247584859844	38.46341656548593	20.22118988544581
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	201.0
1	308.5
2	416.0
3	7378.5
4	14341.0
5	9963.0
6	5585.0
7	4727.0
8	3869.0
9	3671.0
10	3473.0
11	3361.0
12	3249.0
13	3093.0
14	2937.0
15	2784.5
16	2632.0
17	2440.5
18	2249.0
19	2202.0
20	2155.0
21	2132.5
22	2110.0
23	2064.0
24	2018.0
25	2298.5
26	2719.5
27	2860.0
28	3262.0
29	3664.0
30	4079.0
31	4494.0
32	4889.0
33	5284.0
34	5673.0
35	6062.0
36	6254.5
37	6447.0
38	6731.5
39	7016.0
40	7136.0
41	7256.0
42	7586.5
43	7917.0
44	8406.0
45	8895.0
46	9300.0
47	9705.0
48	9052.0
49	8399.0
50	7623.0
51	6847.0
52	5830.5
53	4814.0
54	4188.0
55	3562.0
56	3203.5
57	2845.0
58	2585.0
59	2325.0
60	2099.0
61	1873.0
62	1590.5
63	1308.0
64	1055.0
65	802.0
66	653.0
67	504.0
68	417.5
69	331.0
70	281.5
71	232.0
72	173.5
73	115.0
74	95.0
75	59.0
76	43.0
77	33.0
78	23.0
79	16.5
80	10.0
81	10.5
82	11.0
83	9.0
84	7.0
85	4.5
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	151544.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.53790318323391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.88829792937796	49.77696246634641
2	9.093151184361496	11.191469144274931
3	4.050098115959123	7.477036372274719
4	2.0909958501774666	5.147020007390593
5	1.3114297050087393	4.035131710922241
6	0.8256752844290508	3.0486195428390435
7	0.5125620596845277	2.20793960829858
8	0.34206547497774964	1.6839993665206145
9	0.20909958501774667	1.1580795016628833
>10	0.6358771995667886	5.886079290503088
>50	0.030024555797420034	1.2755371377289764
>100	0.006433833385161436	0.5899276777701525
>500	0.0010723055641935727	0.38206725439476324
>1k	0.0021446111283871454	2.7107638705590453
>5k	0.0010723055641935727	3.4293670485139627
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5197	3.4293670485139627	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2970	1.9598268489679567	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1138	0.750937021591089	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	579	0.38206725439476324	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	233	0.15375072586179592	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCG	154	0.10162065142796811	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.598743599218709E-4	0.0	0.0	0.04289183339492161	0.0
2	6.598743599218709E-4	0.0	0.0	0.2355751464921079	0.0
3	6.598743599218709E-4	0.0	0.0	0.3233384363617167	0.0
4	6.598743599218709E-4	0.0	0.0	0.434197328828591	0.0
5	6.598743599218709E-4	0.0	0.0	0.7826109908673389	0.0
6	6.598743599218709E-4	0.0	0.0	1.0538193527952278	0.0
7	6.598743599218709E-4	0.0	0.0	1.3665997993981946	0.0
8	6.598743599218709E-4	0.0	0.0	1.9756638336060814	0.0
9	6.598743599218709E-4	0.0	0.0	2.2976825212479546	0.0
10	6.598743599218709E-4	0.0	0.0	3.0288233120413874	0.0
11	6.598743599218709E-4	0.0	0.0	3.6708810642453678	0.0
12	6.598743599218709E-4	0.0	0.0	4.369028137042707	0.0
13	6.598743599218709E-4	0.0	0.0	4.607902655334424	0.0
14	6.598743599218709E-4	0.0	0.0	4.721401045240986	0.0
15	6.598743599218709E-4	0.0	0.0	4.888349258301219	0.0
16	6.598743599218709E-4	0.0	0.0	5.1912315895053585	0.0
17	6.598743599218709E-4	0.0	0.0	5.608272184975981	0.0
18	6.598743599218709E-4	0.0	0.0	6.033231272765666	0.0
19	6.598743599218709E-4	0.0	0.0	6.297181016734414	0.0
20	6.598743599218709E-4	0.0	0.0	6.567069629942459	0.0
21	6.598743599218709E-4	0.0	0.0	6.876550704745816	0.0
22	6.598743599218709E-4	0.0	0.0	7.210447130866283	0.0
23	6.598743599218709E-4	0.0	0.0	7.555561421105422	0.0
24	6.598743599218709E-4	0.0	0.0	7.820830913794014	0.0
25	6.598743599218709E-4	0.0	0.0	8.059705432085732	0.0
26	6.598743599218709E-4	0.0	0.0	8.29923982473737	0.0
27	6.598743599218709E-4	0.0	0.0	8.530195850710024	0.0
28	6.598743599218709E-4	0.0	0.0	8.784247479279944	0.0
29	6.598743599218709E-4	0.0	0.0	9.074592197645568	0.0
30	6.598743599218709E-4	0.0	0.0	9.383413398089004	0.0
31	6.598743599218709E-4	0.0	0.0	9.669138995935175	0.0
32	6.598743599218709E-4	0.0	0.0	9.917911629625719	0.0
33	6.598743599218709E-4	0.0	0.0	10.183181122314311	0.0
34	6.598743599218709E-4	0.0	0.0	10.426674761125481	0.0
35	6.598743599218709E-4	0.0	0.0	10.734176212849073	0.0
36	6.598743599218709E-4	0.0	0.0	11.015942564535713	0.0
37	6.598743599218709E-4	0.0	0.0	11.271313941825477	0.0
38	6.598743599218709E-4	0.0	0.0	11.551760544792272	0.0
39	6.598743599218709E-4	0.0	0.0	11.838805891358286	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	20	7.013888E-4	45.000004	2
GGAAGTA	20	7.013888E-4	45.000004	8
CCTCACC	30	2.1529977E-6	45.000004	30
GGGTTGC	20	7.013888E-4	45.000004	8
GCTGTAT	20	7.013888E-4	45.000004	2
ACAACGA	25	3.8747366E-5	44.999996	13
CGTATGG	25	3.8747366E-5	44.999996	2
CAACGAG	25	3.8747366E-5	44.999996	14
CGGTTTA	100	0.0	44.999996	1
CGTTATT	565	0.0	44.999996	1
ACTACGG	25	3.8747366E-5	44.999996	2
GGTATGC	25	3.8747366E-5	44.999996	8
CGAGCGA	25	3.8747366E-5	44.999996	17
CGTTTTA	1110	0.0	44.7973	1
CGTTTTT	2140	0.0	44.579437	1
ATAGGAT	60	3.6379788E-12	41.250004	5
GTTTTAT	1200	0.0	41.25	2
GTTATTT	630	0.0	40.714287	2
GATGCCA	155	0.0	40.64516	9
AGGCGAT	45	1.9093932E-8	40.0	7
>>END_MODULE