##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936161.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	281545
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00710721199098	33.0	31.0	34.0	30.0	34.0
2	32.201917988243444	33.0	31.0	34.0	30.0	34.0
3	32.068315899767356	33.0	31.0	34.0	30.0	34.0
4	35.8275408904438	37.0	35.0	37.0	35.0	37.0
5	32.08177733577226	37.0	35.0	37.0	0.0	37.0
6	33.96697153208191	37.0	35.0	37.0	19.0	37.0
7	20.732476158340585	32.0	0.0	37.0	0.0	37.0
8	28.04951251132146	35.0	17.0	37.0	17.0	37.0
9	34.75545294713101	37.0	32.0	39.0	31.0	39.0
10	36.27574988012574	37.0	35.0	39.0	32.0	39.0
11	36.69860590669342	38.0	35.0	39.0	33.0	39.0
12	37.01088991102666	39.0	37.0	39.0	34.0	39.0
13	36.82786055515104	39.0	37.0	39.0	33.0	39.0
14	38.23996519206521	40.0	38.0	41.0	34.0	41.0
15	38.43347599850823	40.0	38.0	41.0	34.0	41.0
16	38.48474666571951	40.0	38.0	41.0	34.0	41.0
17	38.45828553162017	40.0	38.0	41.0	34.0	41.0
18	38.37419950629562	40.0	38.0	41.0	34.0	41.0
19	38.327961782308336	40.0	37.0	41.0	34.0	41.0
20	38.27646734980198	40.0	37.0	41.0	34.0	41.0
21	38.20063932941448	40.0	37.0	41.0	34.0	41.0
22	38.163398391020976	40.0	37.0	41.0	34.0	41.0
23	38.136013781100715	40.0	37.0	41.0	34.0	41.0
24	38.02594967056776	40.0	36.0	41.0	34.0	41.0
25	37.88332593368733	40.0	36.0	41.0	33.0	41.0
26	37.73061499937843	40.0	36.0	41.0	33.0	41.0
27	37.70163916958213	40.0	36.0	41.0	33.0	41.0
28	37.584144630520875	40.0	36.0	41.0	33.0	41.0
29	37.56604095260083	40.0	36.0	41.0	33.0	41.0
30	37.42745209469179	40.0	36.0	41.0	33.0	41.0
31	37.33648617450141	40.0	36.0	41.0	33.0	41.0
32	37.24467491875189	40.0	35.0	41.0	32.0	41.0
33	37.120573975741	40.0	35.0	41.0	32.0	41.0
34	36.982951215613845	40.0	35.0	41.0	31.0	41.0
35	36.882786055515105	40.0	35.0	41.0	31.0	41.0
36	36.7360528512316	39.0	35.0	41.0	31.0	41.0
37	36.61936457759861	39.0	35.0	41.0	30.0	41.0
38	36.537565930845865	39.0	35.0	41.0	30.0	41.0
39	36.45730522651797	39.0	35.0	41.0	30.0	41.0
40	36.325734784847896	39.0	35.0	41.0	30.0	41.0
41	36.27274148004759	39.0	35.0	41.0	30.0	41.0
42	36.15598572164308	39.0	35.0	40.0	29.0	41.0
43	36.066365945053185	39.0	35.0	40.0	29.0	41.0
44	35.91031984229875	39.0	35.0	40.0	28.0	41.0
45	35.80513949812641	39.0	35.0	40.0	28.0	41.0
46	35.68501305297555	39.0	35.0	40.0	27.0	41.0
47	35.621953861727256	38.0	35.0	40.0	27.0	41.0
48	35.57464703688576	38.0	35.0	40.0	27.0	41.0
49	35.456463442788895	38.0	34.0	40.0	27.0	41.0
50	35.31953328952743	38.0	34.0	40.0	26.0	41.0
51	33.54255980393898	36.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	17.0
10	17.0
11	12.0
12	9.0
13	7.0
14	15.0
15	12.0
16	33.0
17	51.0
18	105.0
19	164.0
20	287.0
21	479.0
22	764.0
23	1187.0
24	1712.0
25	2420.0
26	3074.0
27	3419.0
28	3888.0
29	4493.0
30	5434.0
31	6944.0
32	9224.0
33	13639.0
34	19181.0
35	24511.0
36	30380.0
37	53268.0
38	58053.0
39	38733.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.438651014935445	20.668454421140492	25.351897565220483	13.540996998703584
2	32.447033333925305	26.172725496812234	25.16968868209345	16.210552487169014
3	31.98067804436236	24.50443090802536	27.631106927844574	15.88378411976771
4	29.056456339128733	26.02887637855405	26.803175336091922	18.111491946225293
5	23.12951748388357	38.03015503738301	22.68838018789181	16.151947290841605
6	25.594487559715144	36.06918964996715	24.793549876573906	13.542772913743805
7	47.37395443002006	44.02990640927738	5.767106501624962	2.8290326590775896
8	81.52728693459305	7.1139604681312045	8.21502779307038	3.143724804205367
9	74.82818021985828	9.757942780017403	10.21115629828269	5.202720701841624
10	41.17707648866078	29.42975368058392	16.909907830009413	12.483262000745885
11	32.624980020955796	25.517768029977443	25.672627821485023	16.18462412758174
12	29.800564740982793	24.835461471523203	28.642668134756434	16.721305652737573
13	23.765295068283933	26.688806407501463	30.13656786659326	19.40933065762134
14	19.166030297110588	30.976930863627484	29.012768829139212	20.844270010122713
15	19.538972455557726	27.374309613028114	34.35543163615053	18.731286295263637
16	22.04123674723401	26.200429771439733	32.617521177786855	19.1408123035394
17	21.717309843897066	26.709051838960026	29.41199453018168	22.161643786961232
18	22.344563036104354	28.054485073434087	29.709993073931347	19.890958816530215
19	25.109662753733865	27.05286899074748	27.793780745529133	20.04368750998952
20	25.556127794846294	26.7491875188691	30.175282814470155	17.519401871814452
21	24.09170825267719	28.037436289047935	28.990392299632383	18.880463158642492
22	23.335878811557656	26.642277433447585	29.141700261059512	20.88014349393525
23	23.447761459091797	27.214832442415954	29.44928874602639	19.88811735246586
24	22.432293239091443	26.307695039869294	31.38006357775844	19.879948143280828
25	21.859383047115024	28.23172139444849	29.60734518460637	20.301550373830114
26	21.468326555257597	29.800919923990836	28.278605551510417	20.452147969241153
27	21.928998916691825	29.905343728356037	29.391038732707024	18.77461862224511
28	20.49121810012609	28.96233284199684	31.11012449164432	19.436324566232752
29	21.698485144470688	27.527748672503506	30.283258448915806	20.49050773411
30	21.00126089967856	29.62936653110515	29.943348310216837	19.42602425899945
31	23.894581683212277	28.122325027970664	28.121614661954574	19.861478626862493
32	24.099167095846134	28.798948658296187	27.86623807917029	19.23564616668739
33	21.987959296027277	30.076897121241718	27.56433252233213	20.37081106039887
34	21.528352483617184	28.996075227761104	29.590651583228254	19.884920705393455
35	23.66761974107159	27.838178621534748	27.644603882150275	20.84959775524339
36	23.347244667815094	29.606634818590276	27.759327993748776	19.28679251984585
37	22.191479159637005	30.738958248237402	28.595428794686462	18.47413379743913
38	21.075849331367987	30.301017599318048	28.5371787813671	20.085954287946866
39	23.59445204141434	28.04098811912838	28.22852474737609	20.136035092081194
40	22.541334422561224	28.734305350832017	29.1740219147916	19.550338311815164
41	20.973201442043013	28.172405832104992	29.64215311939477	21.212239606457228
42	21.09112220071392	28.061943916603028	29.203857287467365	21.643076595215685
43	22.396419755278906	27.973148164591805	28.731463886767656	20.89896819336163
44	21.771297661119892	29.43081922960806	27.460619083983023	21.33726402528903
45	21.483954607611572	29.93020653891918	27.090518389600245	21.495320463869007
46	21.545046084995295	31.425527002788183	26.28780479141878	20.74162212079774
47	21.597257987177894	28.47289065691097	30.055230957750982	19.874620398160154
48	21.66438757569838	27.37857180912465	29.89859525120318	21.05844536397379
49	20.479852243868653	28.04525031522492	30.2523575272159	21.22253991369053
50	19.71194658047559	29.583192740059317	29.892557140066415	20.812303539398673
51	20.597417819531515	28.674634605480477	27.834626791454298	22.893320783533717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	201.0
1	564.5
2	928.0
3	2049.5
4	3171.0
5	2240.0
6	1309.0
7	1171.0
8	1033.0
9	972.0
10	911.0
11	876.5
12	842.0
13	900.0
14	958.0
15	977.5
16	997.0
17	957.5
18	918.0
19	1056.5
20	1195.0
21	1719.5
22	2244.0
23	2323.5
24	2403.0
25	3144.5
26	4231.5
27	4577.0
28	5243.5
29	5910.0
30	7079.0
31	8248.0
32	9096.0
33	9944.0
34	10473.5
35	11003.0
36	11635.0
37	12267.0
38	13584.5
39	14902.0
40	16335.0
41	17768.0
42	18210.0
43	18652.0
44	19176.5
45	19701.0
46	19920.0
47	20139.0
48	19383.0
49	18627.0
50	17675.5
51	16724.0
52	15291.5
53	13859.0
54	13031.0
55	12203.0
56	12085.5
57	11968.0
58	11665.5
59	11363.0
60	10720.5
61	10078.0
62	9061.5
63	8045.0
64	6602.0
65	5159.0
66	4334.0
67	3509.0
68	2892.0
69	2275.0
70	1898.0
71	1521.0
72	1218.0
73	915.0
74	732.5
75	434.5
76	319.0
77	231.5
78	144.0
79	111.0
80	78.0
81	58.0
82	38.0
83	29.0
84	20.0
85	17.0
86	14.0
87	13.0
88	12.0
89	11.0
90	10.0
91	6.5
92	3.0
93	3.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	281545.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.88355325081248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.54120671404809	22.209948676055337
2	7.98427340087706	5.25102559093573
3	4.410144520533149	4.35063666554192
4	3.0977943876779506	4.0746594682910375
5	2.4345985180704672	4.002912500665968
6	1.9701453846320018	3.8871228400433324
7	1.619105225637813	3.7269353034150847
8	1.492730768399905	3.9269033369443602
9	1.3371929748763258	3.9574490756362213
>10	8.020997602125684	40.6819513754462
>50	0.05508630187293427	1.249533822301941
>100	0.03456395411635091	2.123639205100428
>500	0.001080123566135966	0.18505034719139035
>1k	0.001080123566135966	0.3722317924310501
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1048	0.3722317924310501	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	521	0.18505034719139035	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	383	0.13603509208119482	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	377	0.13390399403292547	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	346	0.12289332078353372	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	329	0.11685520964677049	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	291	0.10335825534106449	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021666163490738605	0.0
2	0.0	0.0	0.0	0.11188264753414197	0.0
3	0.0	0.0	0.0	0.1381661901294642	0.0
4	0.0	0.0	0.0	0.18576071320748017	0.0
5	0.0	0.0	0.0	0.39922570104246213	0.0
6	0.0	0.0	0.0	0.484469622973237	0.0
7	0.0	0.0	0.0	0.5942211724591095	0.0
8	0.0	0.0	0.0	0.8155001864710792	0.0
9	0.0	0.0	0.0	0.8893782521444175	0.0
10	0.0	0.0	0.0	1.2704896197765898	0.0
11	0.0	0.0	0.0	1.537942424834396	0.0
12	0.0	0.0	0.0	1.9076879362091317	0.0
13	0.0	0.0	0.0	2.0142428386226	0.0
14	0.0	0.0	0.0	2.062902910724751	0.0
15	0.0	0.0	0.0	2.1595126889129626	0.0
16	0.0	0.0	0.0	2.3285798007423324	0.0
17	0.0	0.0	0.0	2.5178923440302614	0.0
18	0.0	0.0	0.0	2.72673995276066	0.0
19	0.0	0.0	0.0	2.8641957768740345	0.0
20	0.0	0.0	0.0	2.990285744729972	0.0
21	0.0	0.0	0.0	3.170363529808734	0.0
22	0.0	0.0	0.0	3.331616615461116	0.0
23	0.0	0.0	0.0	3.492514518105454	0.0
24	0.0	0.0	0.0	3.6555435187980607	0.0
25	0.0	0.0	0.0	3.793709708927525	0.0
26	0.0	0.0	0.0	3.9102097355662506	0.0
27	0.0	0.0	0.0	4.053348487808344	0.0
28	0.0	0.0	0.0	4.200749436146975	0.0
29	0.0	0.0	0.0	4.3424674563568875	0.0
30	0.0	0.0	0.0	4.519348594363246	0.0
31	3.551830080448951E-4	0.0	0.0	4.70688522261095	0.0
32	3.551830080448951E-4	0.0	0.0	4.857482818021986	0.0
33	3.551830080448951E-4	0.0	0.0	5.016604805626099	0.0
34	3.551830080448951E-4	0.0	0.0	5.189223747535918	0.0
35	3.551830080448951E-4	0.0	0.0	5.368235983590545	0.0
36	3.551830080448951E-4	0.0	0.0	5.535882363387736	0.0
37	3.551830080448951E-4	0.0	0.0	5.731943383828518	0.0
38	3.551830080448951E-4	0.0	0.0	5.915572998987728	0.0
39	3.551830080448951E-4	0.0	0.0	6.108082189348061	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	20	7.024203E-4	45.0	4
CATATGC	20	7.024203E-4	45.0	33
GTCGACG	20	7.024203E-4	45.0	10
GGTCGAA	20	7.024203E-4	45.0	6
TTTACGG	25	3.883278E-5	45.0	2
CACGGGC	20	7.024203E-4	45.0	4
ATTACGG	20	7.024203E-4	45.0	2
CGACAAG	20	7.024203E-4	45.0	2
GCACCGA	55	1.8189894E-12	45.0	9
CTGGTAA	20	7.024203E-4	45.0	14
GCCGATA	25	3.883278E-5	45.0	9
CGAATAT	35	1.2075543E-7	45.0	14
CTACCGC	20	7.024203E-4	45.0	22
TAGGACG	20	7.024203E-4	45.0	6
CGTCACG	20	7.024203E-4	45.0	36
TCGGTCA	20	7.024203E-4	45.0	32
ACCTTCG	20	7.024203E-4	45.0	28
CTGGACG	20	7.024203E-4	45.0	1
TACCGCC	20	7.024203E-4	45.0	23
TCACGTC	20	7.024203E-4	45.0	38
>>END_MODULE