##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936160.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	354560
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74948386732852	31.0	31.0	34.0	30.0	34.0
2	31.911033393501803	33.0	31.0	34.0	30.0	34.0
3	31.335178813176896	31.0	31.0	34.0	28.0	34.0
4	35.37690658844765	37.0	35.0	37.0	33.0	37.0
5	31.785080099277977	37.0	35.0	37.0	0.0	37.0
6	33.749018501805054	37.0	35.0	37.0	17.0	37.0
7	21.081557423285197	32.0	0.0	37.0	0.0	37.0
8	28.195388650722023	35.0	17.0	37.0	17.0	37.0
9	34.76372687274368	37.0	32.0	39.0	30.0	39.0
10	36.222766245487364	37.0	35.0	39.0	32.0	39.0
11	36.62980313628159	38.0	35.0	39.0	32.0	39.0
12	37.03531983303249	39.0	37.0	39.0	34.0	39.0
13	36.878170126353794	39.0	37.0	39.0	33.0	39.0
14	38.34476534296029	40.0	38.0	41.0	34.0	41.0
15	38.45774763086643	40.0	38.0	41.0	34.0	41.0
16	38.486639778880864	40.0	38.0	41.0	34.0	41.0
17	38.4109854467509	40.0	38.0	41.0	34.0	41.0
18	38.22175936371841	40.0	37.0	41.0	34.0	41.0
19	38.09034578068592	40.0	37.0	41.0	34.0	41.0
20	37.98885379061372	40.0	36.0	41.0	34.0	41.0
21	37.918944607400725	40.0	36.0	41.0	33.0	41.0
22	37.86138030234657	39.0	36.0	41.0	33.0	41.0
23	37.80353959837545	39.0	36.0	41.0	33.0	41.0
24	37.62381825361011	39.0	35.0	41.0	33.0	41.0
25	37.477174526173286	39.0	35.0	41.0	33.0	41.0
26	37.3185835965704	39.0	35.0	41.0	33.0	41.0
27	37.29406588447653	39.0	35.0	41.0	33.0	41.0
28	37.22214293772563	39.0	35.0	41.0	33.0	41.0
29	37.15230426444043	39.0	35.0	41.0	33.0	41.0
30	36.97799526173285	39.0	35.0	41.0	32.0	41.0
31	36.791964688628155	39.0	35.0	41.0	31.0	41.0
32	36.58986067238267	39.0	35.0	41.0	31.0	41.0
33	36.23808664259928	39.0	35.0	41.0	30.0	41.0
34	35.8968044900722	39.0	35.0	40.0	27.0	41.0
35	35.57159860108303	39.0	35.0	40.0	25.0	41.0
36	35.27536101083032	39.0	35.0	40.0	24.0	41.0
37	35.14223262635379	39.0	35.0	40.0	23.0	41.0
38	35.00228734205776	39.0	34.0	40.0	22.0	41.0
39	34.89557761732852	39.0	34.0	40.0	21.0	41.0
40	34.78433269404332	38.0	34.0	40.0	21.0	41.0
41	34.6558241200361	38.0	34.0	40.0	20.0	41.0
42	34.538315094765345	38.0	34.0	40.0	20.0	41.0
43	34.44387409747292	38.0	34.0	40.0	18.0	41.0
44	34.32918264891697	38.0	33.0	40.0	18.0	41.0
45	34.263024593862816	38.0	33.0	40.0	19.0	41.0
46	34.05872348826715	38.0	33.0	40.0	18.0	41.0
47	33.988171254512636	38.0	33.0	40.0	18.0	41.0
48	33.872512409747294	38.0	33.0	40.0	17.0	41.0
49	33.74034578068592	38.0	33.0	40.0	15.0	41.0
50	33.61587319494585	38.0	33.0	40.0	15.0	41.0
51	31.768462319494585	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	13.0
10	14.0
11	16.0
12	5.0
13	6.0
14	13.0
15	14.0
16	42.0
17	70.0
18	141.0
19	310.0
20	469.0
21	856.0
22	1572.0
23	2613.0
24	4257.0
25	6287.0
26	7627.0
27	7830.0
28	7548.0
29	7476.0
30	8366.0
31	9989.0
32	12639.0
33	17732.0
34	23286.0
35	29711.0
36	37584.0
37	60693.0
38	64621.0
39	42746.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.221908844765345	19.337206678700362	23.56469990974729	19.876184566787003
2	36.9923285198556	25.003666516245488	23.253328068592058	14.750676895306858
3	28.13740974729242	24.627989620938628	32.620430956678696	14.614169675090253
4	24.800315884476536	23.052515794223826	35.71666290613719	16.430505415162454
5	21.479298285198556	34.789316335740075	28.47952391696751	15.251861462093864
6	22.820115072202167	32.23375451263538	32.11416967509025	12.831960740072201
7	43.72602662454874	41.70831453068592	11.912511281588447	2.653147563176895
8	74.5997856498195	6.116595216606498	16.23335965703971	3.050259476534296
9	69.09155009025271	7.472360108303248	17.03519855595668	6.400891245487364
10	34.949796931407946	28.581340252707584	24.2709273465704	12.197935469314078
11	25.465365523465707	24.111574909747294	34.111010830324915	16.312048736462092
12	25.047382671480143	21.759081678700362	36.32445848375451	16.869077166064983
13	20.7454309566787	22.868061823104693	37.87906137184115	18.50744584837545
14	17.608867328519857	26.382558664259925	36.081904332129966	19.926669675090253
15	17.752143501805055	25.087432310469314	40.172326263537904	16.988097924187727
16	20.482287906137184	25.2961416967509	36.14226083032491	18.079309566787003
17	20.048510830324908	24.765060920577618	36.15354241877256	19.03288583032491
18	20.04907490974729	25.923680054151628	35.476365072202164	18.550879963898918
19	21.105595667870038	27.37138989169675	33.17999774368231	18.3430166967509
20	22.577278880866423	25.27555279783393	35.55533619133574	16.591832129963898
21	21.366764440433215	26.628215252707584	35.04681859205776	16.958201714801444
22	20.583822202166065	23.599390794223826	35.476365072202164	20.340421931407942
23	19.78875225631769	26.173285198555956	36.34250902527076	17.695453519855594
24	20.2961416967509	23.859149368231048	36.233923736462096	19.61078519855596
25	19.57553023465704	27.24870261732852	34.44297157039711	18.732795577617328
26	18.550597924187727	26.721288357400724	34.910029332129966	19.81808438628159
27	19.163188176895307	27.32851985559567	34.485559566787	19.022732400722024
28	17.6821976534296	26.891922382671478	36.97822653429603	18.44765342960289
29	19.922721119133573	24.490354241877256	36.38650722021661	19.200417418772563
30	18.651850180505416	25.879399819494587	35.8757333032491	19.593016696750905
31	20.775609205776174	24.7492666967509	35.160198555956676	19.314925541516246
32	22.659352436823106	25.458878610108304	33.79992102888087	18.081847924187723
33	20.130584386281587	26.679828519855597	33.36529783393502	19.824289259927795
34	19.675936371841154	26.058495036101082	35.20278655234657	19.06278203971119
35	19.840929602888085	25.716944945848375	34.08816561371842	20.353959837545126
36	20.29134702166065	28.258122743682314	32.664429151624546	18.78610108303249
37	20.71158619133574	28.187612815884478	33.20763763537906	17.89316335740072
38	20.04258799638989	28.68879738267148	31.870487364620935	19.39812725631769
39	21.654162906137184	26.960740072202167	31.57321750902527	19.81187951263538
40	20.879399819494584	26.993738718411553	33.18394629963899	18.942915162454874
41	19.33946299638989	27.564587093862812	32.44528429602888	20.65066561371841
42	19.210852888086645	27.071581678700362	33.125	20.592565433212997
43	21.166516245487365	26.50750225631769	31.28130640794224	21.044675090252706
44	20.412906137184113	26.899255415162454	31.664880415162454	21.022958032490973
45	19.74870261732852	27.029839801444044	31.337150270758123	21.884307310469314
46	20.748533393501805	29.023578519855597	29.8601083032491	20.3677797833935
47	19.39389666064982	26.532885830324908	34.04698781588448	20.02622969314079
48	20.00817915162455	26.506938176895307	32.670069945848375	20.814812725631768
49	19.027809115523468	26.21164259927798	33.79681859205776	20.963729693140795
50	18.53029106498195	26.48691335740072	33.03136281588447	21.951432761732853
51	18.34019629963899	26.094596119133573	32.36377481949459	23.20143276173285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	418.0
1	642.0
2	866.0
3	7522.0
4	14178.0
5	9674.0
6	5170.0
7	4477.0
8	3784.0
9	3551.5
10	3319.0
11	3215.0
12	3111.0
13	2964.0
14	2817.0
15	2699.5
16	2582.0
17	2478.0
18	2374.0
19	2397.0
20	2420.0
21	2703.5
22	2987.0
23	3078.5
24	3170.0
25	3600.5
26	4650.5
27	5270.0
28	6233.5
29	7197.0
30	8314.0
31	9431.0
32	10480.5
33	11530.0
34	12586.0
35	13642.0
36	14395.5
37	15149.0
38	15981.0
39	16813.0
40	17686.0
41	18559.0
42	19451.5
43	20344.0
44	22372.5
45	24401.0
46	26850.0
47	29299.0
48	26063.0
49	22827.0
50	21355.5
51	19884.0
52	18041.0
53	16198.0
54	15013.5
55	13829.0
56	13439.5
57	13050.0
58	12761.0
59	12472.0
60	11676.5
61	10881.0
62	9597.0
63	8313.0
64	6830.5
65	5348.0
66	4364.0
67	3380.0
68	2788.5
69	2197.0
70	1791.0
71	1385.0
72	1073.5
73	762.0
74	638.0
75	424.5
76	335.0
77	265.5
78	196.0
79	126.0
80	56.0
81	44.5
82	33.0
83	24.5
84	16.0
85	12.0
86	8.0
87	6.5
88	5.0
89	4.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	354560.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.007678973931284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94671812491778	33.56109189693072
2	9.31136406241216	8.567884971857186
3	4.688975021823031	6.471865725624493
4	3.0146475152856977	5.54787740411296
5	2.175375748288773	5.004199453747271
6	1.6692965693378838	4.6080276404629315
7	1.328455665583088	4.2783413255272835
8	1.0619014154108768	3.9084495537749486
9	0.8281021519892678	3.42891521697095
>10	2.929632207789018	18.39289679572721
>50	0.025572495940575923	0.8138152285965758
>100	0.013721827090065131	1.2296185664796642
>500	0.0031185970659238934	0.9541380941761688
>1k	0.002494877652739115	1.7742664169296998
>5k	6.237194131847787E-4	1.4586117090819446
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5083	1.4336078519855597	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2853	0.8046592960288809	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG	1132	0.31926895306859204	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	1128	0.31814079422382674	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1070	0.30178249097472926	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC	818	0.2307084837545126	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG	751	0.21181182310469315	No Hit
GAATCTATCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	670	0.18896660649819494	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	546	0.1539936823104693	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC	540	0.1523014440433213	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03694720216606498	0.0
2	0.0	0.0	0.0	0.21209386281588447	0.0
3	0.0	0.0	0.0	0.2842960288808664	0.0
4	0.0	0.0	0.0	0.4614169675090253	0.0
5	0.0	0.0	0.0	1.1027752707581226	0.0
6	0.0	0.0	0.0	1.3413808664259927	0.0
7	0.0	0.0	0.0	1.6299074909747293	0.0
8	0.0	0.0	0.0	2.082299187725632	0.0
9	0.0	0.0	0.0	2.284239620938628	0.0
10	0.0	0.0	0.0	3.2970442238267146	0.0
11	0.0	0.0	0.0	3.6772337545126352	0.0
12	0.0	0.0	0.0	4.4328181407942235	0.0
13	0.0	0.0	0.0	4.6107851985559565	0.0
14	0.0	0.0	0.0	4.712601534296029	0.0
15	0.0	0.0	0.0	4.880979241877256	0.0
16	0.0	0.0	0.0	5.097021660649819	0.0
17	0.0	0.0	0.0	5.3483190433212995	0.0
18	0.0	0.0	0.0	5.652075812274369	0.0
19	0.0	0.0	0.0	5.8359657039711195	0.0
20	0.00197427797833935	0.0	0.0	6.024650270758123	0.0
21	0.00197427797833935	0.0	0.0	6.264948104693141	0.0
22	0.00197427797833935	0.0	0.0	6.526962996389892	0.0
23	0.00197427797833935	0.0	0.0	6.790952166064982	0.0
24	0.00197427797833935	0.0	0.0	6.999097472924188	0.0
25	0.00197427797833935	0.0	0.0	7.192576714801444	0.0
26	0.00197427797833935	0.0	0.0	7.371953971119134	0.0
27	0.00197427797833935	0.0	0.0	7.560638537906137	0.0
28	0.00197427797833935	0.0	0.0	7.766809566787003	0.0
29	0.00197427797833935	0.0	0.0	7.998928249097473	0.0
30	0.00197427797833935	0.0	0.0	8.262353339350181	0.0
31	0.00197427797833935	0.0	0.0	8.484600631768952	0.0
32	0.00197427797833935	0.0	0.0	8.750846119133573	0.0
33	0.00197427797833935	0.0	0.0	8.999041064981949	0.0
34	0.00197427797833935	0.0	0.0	9.220160198555957	0.0
35	0.00197427797833935	0.0	0.0	9.477662454873647	0.0
36	0.00197427797833935	0.0	0.0	9.725857400722022	0.0
37	0.00197427797833935	0.0	0.0	9.993513086642599	0.0
38	0.00197427797833935	0.0	0.0	10.268783844765343	0.0
39	0.00197427797833935	0.0	0.0	10.544336642599278	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTACC	35	1.2087548E-7	45.0	7
TGTTACG	20	7.0266804E-4	45.0	1
CGAATAT	95	0.0	45.0	14
CGGGACG	25	3.8853308E-5	45.0	6
GTTCCCT	20	7.0266804E-4	45.0	27
GCTAGCG	20	7.0266804E-4	45.0	1
TATAGCG	20	7.0266804E-4	45.0	1
CGTTATT	420	0.0	44.464287	1
CGTTTTA	1030	0.0	44.126213	1
CGGTTTA	120	0.0	43.125004	1
TACGAAT	100	0.0	42.75	12
GCTACGA	100	0.0	42.75	10
CGTTTTT	2005	0.0	42.531174	1
AAACGGC	70	0.0	41.785713	6
TACGGCT	120	0.0	41.250004	7
CTATGCT	100	0.0	40.5	36
ACGGCTG	185	0.0	40.135136	8
TACGGGA	135	0.0	40.0	4
ACGGGAT	175	0.0	39.857143	5
TGACGGG	85	0.0	39.705883	3
>>END_MODULE