FastQCFastQC Report
Sat 14 Jan 2017
SRR2936159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671210
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35720.5321732393736684No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21440.3194231313597831No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA207.031186E-445.00000423
CTAACCG253.8890652E-545.027
CGTTTTA5600.041.7857131
TTTACGG603.6379788E-1241.2500042
ATTTACG403.4553523E-739.3750041
CGTTTTT12650.039.30831
CGCATAG356.244405E-638.5714262
AATAGGC659.094947E-1238.076924
CGAAGGA1300.038.076924
CGAATAT950.037.89473714
CGGGTAT301.1394156E-437.5000046
TCGATAG301.1394156E-437.5000042
CGAGGTA301.1394156E-437.5000045
TAGGTTA552.744855E-936.818186
AGGCGAT3600.036.257
AACGTCC250.002106485436.038
CACGGTA250.002106485436.028
TTTCGCG250.002106485436.01
ACCGACA250.002106485436.028
TATGCGA250.002106485436.01
Produced by FastQC (version 0.11.5)