FastQCFastQC Report
Sat 14 Jan 2017
SRR2936158.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936158.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414218
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36270.8756258781607752No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.4895972652081754No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.20061899772583516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG253.8864706E-545.02
CATGCGG207.028056E-445.02
CGTAAAG406.7975634E-945.02
ACGTAAA253.8864706E-545.01
CGTTTAG302.1621272E-644.9999962
AGGCGAT2250.043.07
CGTTTTA7150.042.7972031
CGTTATT2900.041.896551
TTAGGAC603.6379788E-1241.2499965
CGTTTTT11850.041.202531
CGAATAT850.039.70588314
TATACTG800.039.37518
ACGGGCA700.038.5714265
CACGGGT301.1386616E-437.4999964
CGATTGA301.1386616E-437.49999610
CTAGGCG301.1386616E-437.4999965
ATGGGCG552.739398E-936.818185
ACGGGCG552.739398E-936.818185
GTCTTAC552.739398E-936.8181831
TTACACG552.739398E-936.8181834
Produced by FastQC (version 0.11.5)