##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936158.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	414218
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.943606989556223	31.0	31.0	34.0	30.0	34.0
2	32.13580771477821	33.0	31.0	34.0	30.0	34.0
3	31.830132925174667	33.0	31.0	34.0	30.0	34.0
4	35.69158752154662	37.0	35.0	37.0	35.0	37.0
5	32.088434109575154	37.0	35.0	37.0	10.0	37.0
6	33.93368226392865	37.0	35.0	37.0	19.0	37.0
7	20.701635370746803	32.0	0.0	37.0	0.0	37.0
8	28.010129931581922	35.0	17.0	37.0	17.0	37.0
9	34.7378216301561	37.0	32.0	39.0	31.0	39.0
10	36.24042412449483	37.0	35.0	39.0	32.0	39.0
11	36.66843787570796	38.0	35.0	39.0	33.0	39.0
12	36.995454084564166	39.0	37.0	39.0	33.0	39.0
13	36.786370944768215	39.0	37.0	39.0	33.0	39.0
14	38.13322453394107	40.0	38.0	41.0	33.0	41.0
15	38.31404236416573	40.0	38.0	41.0	34.0	41.0
16	38.40716965462631	40.0	38.0	41.0	34.0	41.0
17	38.38665871594185	40.0	38.0	41.0	34.0	41.0
18	38.261625520860996	40.0	37.0	41.0	34.0	41.0
19	38.18908159471582	40.0	37.0	41.0	34.0	41.0
20	38.108689144363595	40.0	37.0	41.0	34.0	41.0
21	38.02227570989189	40.0	37.0	41.0	33.0	41.0
22	37.96963434713122	40.0	36.0	41.0	34.0	41.0
23	37.901167984008424	40.0	36.0	41.0	33.0	41.0
24	37.76088436523763	40.0	36.0	41.0	33.0	41.0
25	37.59709621503653	40.0	35.0	41.0	33.0	41.0
26	37.45664843150225	39.0	35.0	41.0	33.0	41.0
27	37.40908169128333	39.0	35.0	41.0	33.0	41.0
28	37.29054507529852	39.0	35.0	41.0	33.0	41.0
29	37.24152499408524	39.0	35.0	41.0	33.0	41.0
30	37.07059567667267	39.0	35.0	41.0	32.0	41.0
31	36.92185274420716	39.0	35.0	41.0	31.0	41.0
32	36.78268930852836	39.0	35.0	41.0	31.0	41.0
33	36.55807328508177	39.0	35.0	41.0	30.0	41.0
34	36.31108498423536	39.0	35.0	41.0	30.0	41.0
35	36.10040848055855	39.0	35.0	41.0	29.0	41.0
36	35.91332100488149	39.0	35.0	40.0	28.0	41.0
37	35.8186800187341	39.0	35.0	40.0	27.0	41.0
38	35.67526761270635	39.0	35.0	40.0	27.0	41.0
39	35.565385859619816	39.0	35.0	40.0	26.0	41.0
40	35.4242959021578	39.0	35.0	40.0	25.0	41.0
41	35.29684610519099	38.0	35.0	40.0	25.0	41.0
42	35.17599186901583	38.0	34.0	40.0	24.0	41.0
43	35.11455320628268	38.0	34.0	40.0	24.0	41.0
44	34.978607882805676	38.0	34.0	40.0	24.0	41.0
45	34.90715999787552	38.0	34.0	40.0	23.0	41.0
46	34.78988841624459	38.0	34.0	40.0	23.0	41.0
47	34.71851778532077	38.0	34.0	40.0	23.0	41.0
48	34.60286853782308	38.0	34.0	40.0	23.0	41.0
49	34.498097620093766	38.0	33.0	40.0	23.0	41.0
50	34.33927786817569	37.0	33.0	40.0	23.0	41.0
51	32.62201304627032	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	23.0
10	31.0
11	19.0
12	11.0
13	12.0
14	19.0
15	29.0
16	49.0
17	101.0
18	214.0
19	355.0
20	585.0
21	1016.0
22	1617.0
23	2498.0
24	3780.0
25	5407.0
26	6283.0
27	6715.0
28	6985.0
29	7654.0
30	8865.0
31	11016.0
32	14665.0
33	21484.0
34	29138.0
35	37074.0
36	46698.0
37	76261.0
38	76630.0
39	48970.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.575098136729935	20.400610306650123	24.72031635515598	16.303975201463963
2	34.5013978146773	24.33428774220338	24.850923909632126	16.313390533487198
3	31.169335953531714	23.18127169751194	29.89078214853048	15.758610200425863
4	27.86165738813861	24.9981893592263	29.432569323399754	17.70758392923533
5	22.11033803456151	36.01968045811626	25.277269457145756	16.592712050176477
6	25.09185984191899	33.93913349975134	27.612996055217305	13.35601060311237
7	46.41855254962363	43.24413714517477	7.713088277187374	2.624222028014234
8	80.99672153310576	5.209817052856225	10.853946472630355	2.9395149414076647
9	74.91707265256458	7.541680950610548	12.805575807907912	4.735670588916947
10	42.040664577589574	24.82919622034774	20.04669039008445	13.083448811978235
11	31.04910940615811	24.98853260843324	27.19799718988552	16.76436079552313
12	28.64795832146358	22.563963903065535	31.187200942498876	17.60087683297201
13	24.064381557537335	23.505255686619122	32.44088861420798	19.98947414163556
14	20.073487873535193	25.890473132505104	33.18469984404347	20.851339149916228
15	20.186471857814002	24.71331521083101	35.85937839495145	19.240834536403536
16	23.666282006093407	23.934256840600842	33.20594469578821	19.19351645751754
17	23.976505125320482	24.068968514164037	31.156057921191255	20.79846843932422
18	24.243272865978785	25.403290054995196	30.86200985954256	19.49142721948346
19	24.951595536649783	25.91582210333689	29.38162996296636	19.750952397046966
20	26.004422791863224	25.809597844613226	30.00738741435669	18.178591949166865
21	25.594252301927973	25.63432781771917	30.461496120400366	18.30992375995249
22	24.796121848881505	23.693803745853632	31.090874853338097	20.419199551926763
23	23.405308315910943	25.12324428199644	31.486318798313935	19.985128603778687
24	23.1790989285835	24.821229400943466	31.88562544360699	20.114046226866048
25	23.0361790168462	26.49184728814296	29.729514410286374	20.742459284724468
26	22.242394101656615	27.23831412444655	29.61797893862652	20.901312835270318
27	21.814841460293856	26.236426229666503	31.383474402367835	20.565257907671803
28	21.104587439464243	27.038902220569845	31.61113230231424	20.24537803765167
29	22.096094327141746	26.113785494594634	30.98682336354287	20.803296814720753
30	22.731749948094965	26.302333553829143	30.917053339063006	20.048863159012885
31	24.102525723169926	26.054396477217313	30.060740962488353	19.78233683712441
32	24.868547479829463	26.378380466324497	28.903137961170206	19.849934092675838
33	23.998715652144522	26.479534930881805	29.517790149148514	20.003959267825156
34	22.867185877967643	26.58672486468478	30.745646012486176	19.800443244861402
35	23.481355228406297	27.616858755534523	29.257057877735875	19.6447281383233
36	24.005475377699664	28.594121935792266	27.519084153754786	19.881318532753284
37	23.25297307215041	28.74066312907696	28.326871357594307	19.67949244117832
38	23.44176255015475	27.93046173753917	28.773254663003538	19.85452104930254
39	23.274942180204626	27.478767219193756	28.57794687821389	20.66834372238773
40	24.21381977605995	26.580689395439116	29.445847355740213	19.75964347276072
41	21.823291117237783	27.109637920129014	29.286993805194367	21.78007715743884
42	21.69099363137285	27.611306123828516	29.219879387182594	21.477820857616038
43	23.044387255020304	26.676291228290417	28.8466459690308	21.43267554765848
44	22.92947192058288	26.977340434264086	28.929935444620945	21.16325220053209
45	22.020771670955874	27.212240897305286	28.088832450545365	22.678154981193476
46	22.12699592967954	28.27351780946265	28.182744352007884	21.41674190884993
47	21.647055415264425	27.213447991154418	30.943367984974095	20.19612860860706
48	22.213182430507608	26.574895344963277	30.22273295704194	20.98918926748717
49	21.744105760734687	26.807623039076038	29.87146864694436	21.57680255324491
50	20.954666383401978	27.823996060045676	30.112404579231228	21.10893297732112
51	21.195360896919013	27.019347300213898	29.45308991883501	22.33220188403208
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	314.0
1	729.5
2	1145.0
3	5640.0
4	10135.0
5	6699.5
6	3264.0
7	2804.5
8	2345.0
9	2351.0
10	2357.0
11	2239.5
12	2122.0
13	2072.0
14	2022.0
15	1965.5
16	1909.0
17	1831.5
18	1754.0
19	1925.5
20	2097.0
21	2214.0
22	2331.0
23	2816.5
24	3302.0
25	3901.5
26	4947.5
27	5394.0
28	6444.5
29	7495.0
30	8740.0
31	9985.0
32	11205.0
33	12425.0
34	13604.0
35	14783.0
36	15164.0
37	15545.0
38	16910.5
39	18276.0
40	19368.5
41	20461.0
42	21038.5
43	21616.0
44	22610.0
45	23604.0
46	24519.0
47	25434.0
48	24671.5
49	23909.0
50	23720.0
51	23531.0
52	22705.5
53	21880.0
54	21652.5
55	21425.0
56	21594.5
57	21764.0
58	21604.5
59	21445.0
60	20004.5
61	18564.0
62	16931.0
63	15298.0
64	13151.0
65	11004.0
66	9118.5
67	7233.0
68	6125.5
69	5018.0
70	4218.5
71	3419.0
72	2774.0
73	2129.0
74	1699.5
75	1015.0
76	760.0
77	631.5
78	503.0
79	363.0
80	223.0
81	165.5
82	108.0
83	80.5
84	53.0
85	42.5
86	32.0
87	23.5
88	15.0
89	13.0
90	11.0
91	7.5
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	414218.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.88101434467972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.87532349516475	28.974551164896457
2	8.524759091083366	6.970015973750359
3	4.231432979764584	5.189558170329205
4	2.925113096374991	4.783263618108661
5	2.2795230738735883	4.65946077410273
6	1.9254352904646308	4.722824863754229
7	1.6300538947146639	4.664677965670186
8	1.386649159107462	4.535009933160818
9	1.1681238416530393	4.297867877428225
>10	5.020540445364104	28.2690214586812
>50	0.019827379460901087	0.5440599684579058
>100	0.011415763932033954	0.7965627438866764
>500	6.00829680633366E-4	0.2041145976471872
>1k	0.001201659361266732	1.3890108901261655
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3627	0.8756258781607752	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2028	0.4895972652081754	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	831	0.20061899772583516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011105263412019757	0.0
2	0.0	0.0	0.0	0.06349313646437384	0.0
3	0.0	0.0	0.0	0.0980160205495657	0.0
4	0.0	0.0	0.0	0.1421956554278182	0.0
5	0.0	0.0	0.0	0.30008353089435996	0.0
6	0.0	0.0	0.0	0.4106533274749045	0.0
7	0.0	0.0	0.0	0.5023924600089807	0.0
8	0.0	0.0	0.0	0.6711441801177158	0.0
9	0.0	0.0	0.0	0.7645732440405777	0.0
10	0.0	0.0	0.0	1.026512609302348	0.0
11	0.0	0.0	0.0	1.2061281740532763	0.0
12	0.0	0.0	0.0	1.429440536142804	0.0
13	0.0	0.0	0.0	1.5117643366536462	0.0
14	0.0	0.0	0.0	1.5641522097060003	0.0
15	0.0	0.0	0.0	1.6158158264488747	0.0
16	0.0	0.0	0.0	1.7092448903717365	0.0
17	0.0	0.0	0.0	1.8072609109213023	0.0
18	0.0	0.0	0.0	1.932798671231091	0.0
19	0.0	0.0	0.0	2.004741464639393	0.0
20	0.0	0.0	0.0	2.0788570269761335	0.0
21	0.0	0.0	0.0	2.1734931847481276	0.0
22	0.0	0.0	0.0	2.2775446745433565	0.0
23	0.0	0.0	0.0	2.3791819766403197	0.0
24	0.0	0.0	0.0	2.463437127309774	0.0
25	2.4141876982651647E-4	0.0	0.0	2.5440709964318304	0.0
26	2.4141876982651647E-4	0.0	0.0	2.622290677855622	0.0
27	2.4141876982651647E-4	0.0	0.0	2.7258593301111973	0.0
28	2.4141876982651647E-4	0.0	0.0	2.817115625105621	0.0
29	2.4141876982651647E-4	0.0	0.0	2.914407389345707	0.0
30	2.4141876982651647E-4	0.0	0.0	3.0327025865607	0.0
31	2.4141876982651647E-4	0.0	0.0	3.135546982506796	0.0
32	2.4141876982651647E-4	0.0	0.0	3.241529822460637	0.0
33	2.4141876982651647E-4	0.0	0.0	3.3460641497955184	0.0
34	2.4141876982651647E-4	0.0	0.0	3.45542685252693	0.0
35	2.4141876982651647E-4	0.0	0.0	3.570825024504005	0.0
36	2.4141876982651647E-4	0.0	0.0	3.698052716202579	0.0
37	2.4141876982651647E-4	0.0	0.0	3.822141963893409	0.0
38	2.4141876982651647E-4	0.0	0.0	3.9761671390427265	0.0
39	2.4141876982651647E-4	0.0	0.0	4.217344490099416	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	25	3.8864706E-5	45.0	2
CATGCGG	20	7.028056E-4	45.0	2
CGTAAAG	40	6.7975634E-9	45.0	2
ACGTAAA	25	3.8864706E-5	45.0	1
CGTTTAG	30	2.1621272E-6	44.999996	2
AGGCGAT	225	0.0	43.0	7
CGTTTTA	715	0.0	42.797203	1
CGTTATT	290	0.0	41.89655	1
TTAGGAC	60	3.6379788E-12	41.249996	5
CGTTTTT	1185	0.0	41.20253	1
CGAATAT	85	0.0	39.705883	14
TATACTG	80	0.0	39.375	18
ACGGGCA	70	0.0	38.571426	5
CACGGGT	30	1.1386616E-4	37.499996	4
CGATTGA	30	1.1386616E-4	37.499996	10
CTAGGCG	30	1.1386616E-4	37.499996	5
ATGGGCG	55	2.739398E-9	36.81818	5
ACGGGCG	55	2.739398E-9	36.81818	5
GTCTTAC	55	2.739398E-9	36.81818	31
TTACACG	55	2.739398E-9	36.81818	34
>>END_MODULE