##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936157.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	182451
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.895714465801777	31.0	31.0	34.0	30.0	34.0
2	32.0955379800604	33.0	31.0	34.0	30.0	34.0
3	31.862642572526322	33.0	31.0	34.0	30.0	34.0
4	35.68061561734383	37.0	35.0	37.0	35.0	37.0
5	31.951214298633605	37.0	35.0	37.0	0.0	37.0
6	33.876750469989204	37.0	35.0	37.0	19.0	37.0
7	20.94076217724211	32.0	0.0	37.0	0.0	37.0
8	28.1762390998131	35.0	17.0	37.0	17.0	37.0
9	34.78774575091394	37.0	32.0	39.0	31.0	39.0
10	36.21300513562546	37.0	35.0	39.0	32.0	39.0
11	36.636488701075905	38.0	35.0	39.0	33.0	39.0
12	36.97064965387967	39.0	37.0	39.0	33.0	39.0
13	36.62255619316967	39.0	35.0	39.0	33.0	39.0
14	38.01124137439641	40.0	37.0	41.0	33.0	41.0
15	38.237899490822194	40.0	37.0	41.0	33.0	41.0
16	38.33786605718796	40.0	37.0	41.0	34.0	41.0
17	38.315103781289224	40.0	37.0	41.0	34.0	41.0
18	38.202646189935926	40.0	37.0	41.0	34.0	41.0
19	38.12344684326202	40.0	37.0	41.0	34.0	41.0
20	38.09042427829938	40.0	37.0	41.0	34.0	41.0
21	38.00116195581279	40.0	36.0	41.0	34.0	41.0
22	37.93569232287025	40.0	36.0	41.0	33.0	41.0
23	37.88629275805559	40.0	36.0	41.0	34.0	41.0
24	37.7235476922571	40.0	35.0	41.0	33.0	41.0
25	37.63050901337893	39.0	35.0	41.0	33.0	41.0
26	37.45938361532686	39.0	35.0	41.0	33.0	41.0
27	37.40357685077089	39.0	35.0	41.0	33.0	41.0
28	37.348263369343	39.0	35.0	41.0	33.0	41.0
29	37.31725230335816	39.0	35.0	41.0	33.0	41.0
30	37.16447703767039	39.0	35.0	41.0	32.0	41.0
31	37.03399268844786	39.0	35.0	41.0	32.0	41.0
32	36.89462924292002	39.0	35.0	41.0	31.0	41.0
33	36.7053071783657	39.0	35.0	41.0	31.0	41.0
34	36.531172753232376	39.0	35.0	41.0	30.0	41.0
35	36.31647401220054	39.0	35.0	41.0	30.0	41.0
36	36.163073921217205	39.0	35.0	41.0	30.0	41.0
37	36.057571621969736	39.0	35.0	41.0	29.0	41.0
38	35.96424793506202	39.0	35.0	40.0	29.0	41.0
39	35.909526393387814	39.0	35.0	40.0	28.0	41.0
40	35.760258918833	39.0	35.0	40.0	27.0	41.0
41	35.67388504310746	39.0	35.0	40.0	27.0	41.0
42	35.49947657179188	38.0	35.0	40.0	26.0	41.0
43	35.36826325972453	38.0	35.0	40.0	26.0	41.0
44	35.17321362996092	38.0	34.0	40.0	24.0	41.0
45	35.08459805646447	38.0	34.0	40.0	24.0	41.0
46	34.9626475053576	38.0	34.0	40.0	24.0	41.0
47	34.91299581805526	38.0	34.0	40.0	23.0	41.0
48	34.785098464793286	38.0	34.0	40.0	23.0	41.0
49	34.66975790760259	38.0	34.0	40.0	23.0	41.0
50	34.514477859808935	38.0	34.0	40.0	23.0	41.0
51	32.563855500929016	36.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	11.0
10	9.0
11	2.0
12	2.0
13	8.0
14	7.0
15	11.0
16	15.0
17	36.0
18	58.0
19	135.0
20	216.0
21	404.0
22	635.0
23	1006.0
24	1444.0
25	2036.0
26	2483.0
27	2770.0
28	2970.0
29	3412.0
30	3778.0
31	5036.0
32	6663.0
33	9775.0
34	13573.0
35	16990.0
36	19564.0
37	32973.0
38	34369.0
39	22053.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.13022674581121	20.72337230270045	25.0275416413174	14.11885931017095
2	33.43966325205124	24.71403280880894	25.21224876816241	16.634055170977412
3	30.74743355750311	23.299954508333744	30.07766468805323	15.874947246109913
4	26.91078700582622	24.45916985930469	30.391721612926208	18.238321521942876
5	22.311195882730157	35.873741442907956	25.824467939337136	15.990594735024747
6	25.000137023091128	33.530646584562426	27.94558539004993	13.523631002296508
7	47.319554291289165	42.61034469528805	7.489134068873287	2.5809669445494956
8	81.02449424777063	5.321428767175845	10.187940871795716	3.4661361132578064
9	74.28131388701624	7.062170116908101	12.315635430882812	6.340880565192847
10	40.97976991082537	25.464371255844036	20.379170297778582	13.176688535552012
11	30.29580544913429	25.461082701656885	28.433387594477423	15.809724254731409
12	30.045875330910764	21.89628996278453	30.307863481153845	17.749971225150862
13	22.98644567582529	27.602479569857113	32.02394067448247	17.387134079835135
14	18.420288186965266	29.48353256490784	31.693440978673728	20.40273826945317
15	18.044296824900933	25.799803782933502	37.798093734756186	18.357805657409386
16	20.32984198497131	23.952184422118815	35.87483762763701	19.843135965272868
17	20.837375514521707	23.663339746013996	32.1527423801459	23.34654235931839
18	21.14321105392681	24.74088933467068	33.807981321012214	20.307918290390297
19	22.79899808715765	25.23417246274342	31.385961162175054	20.58086828792388
20	24.408745361768368	25.310905393776963	31.391990178184827	18.888359066269846
21	23.463286032962273	26.228412011992262	31.002296507007358	19.306005448038103
22	22.020706929531762	24.92614455388022	30.977084258239195	22.07606425834882
23	21.272012759590247	25.542748463971147	31.938986357981047	21.24625241845756
24	21.110873604419815	23.97849285561603	34.56982970770234	20.340803832261813
25	20.608272906150145	26.903661805087392	30.489282053811706	21.998783234950753
26	19.772980142613633	27.94777775950803	30.7753862680939	21.503855829784435
27	19.222147316265737	29.15686951565078	31.584370598133198	20.03661256995029
28	18.38137362908397	27.63207655754148	33.80578895155411	20.180760861820435
29	19.399729242371926	26.513968133909927	33.64464979638369	20.44165282733446
30	19.500578237444575	27.132764413458954	33.476933532838956	19.889723816257515
31	22.18568273125387	25.843651172095523	31.581082043946047	20.389584052704564
32	23.422179105622877	27.230872946708978	30.59835243435224	18.748595513315905
33	22.427391464009517	27.378309792766274	30.26291990726277	19.931378835961436
34	21.713775205397614	26.271711308789758	30.57971729395838	21.434796191854254
35	20.128143994826008	27.243479071093063	31.02805684814005	21.600320085940883
36	22.815440858093407	26.60714383587922	30.54244701317066	20.03496829285671
37	21.202405029295537	28.35720275580841	31.42761618187897	19.012776033017083
38	19.555387473897103	28.156052858027635	29.934064488547612	22.354495179527653
39	21.66335070786129	27.28184553660983	29.900630854311565	21.154172901217315
40	21.99220612657645	25.973000970123483	32.134655332116566	19.900137571183496
41	20.768863968956047	25.938471151158392	30.69974952178941	22.592915358096146
42	21.658965968945086	25.53069043195159	30.472839282875952	22.33750431622737
43	22.54961606129865	26.589604880214413	29.38049120037709	21.480287858109847
44	20.609369090879195	27.244027163457584	29.615622824758432	22.530980920904792
45	19.91603224975473	29.13494582106977	28.830754558758244	22.118267370417264
46	22.027284037906067	28.34459663142433	28.53533277427912	21.092786556390482
47	20.75022882856219	27.404070133898966	30.73866407967071	21.10703695786814
48	21.535645186926903	26.634000361740963	30.914053636318794	20.916300815013347
49	20.5704545329979	25.5416522792421	31.152473814887284	22.735419372872716
50	19.409594904933382	27.101523148681018	31.08615463877973	22.402727307605875
51	19.518117193109383	27.527939008281678	29.36021178288965	23.59373201571929
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	185.0
1	341.0
2	497.0
3	1832.0
4	3167.0
5	2203.0
6	1239.0
7	1073.0
8	907.0
9	900.0
10	893.0
11	897.5
12	902.0
13	877.0
14	852.0
15	858.5
16	865.0
17	868.5
18	872.0
19	957.5
20	1043.0
21	1084.0
22	1125.0
23	1251.5
24	1378.0
25	1598.5
26	2115.0
27	2411.0
28	2920.5
29	3430.0
30	4006.5
31	4583.0
32	4982.5
33	5382.0
34	6049.5
35	6717.0
36	7150.5
37	7584.0
38	8175.0
39	8766.0
40	9726.5
41	10687.0
42	11699.5
43	12712.0
44	13274.0
45	13836.0
46	14338.5
47	14841.0
48	13686.0
49	12531.0
50	12067.5
51	11604.0
52	10756.5
53	9909.0
54	9295.0
55	8681.0
56	8038.0
57	7395.0
58	7124.0
59	6853.0
60	6210.5
61	5568.0
62	5032.5
63	4497.0
64	3718.5
65	2940.0
66	2474.0
67	2008.0
68	1682.0
69	1356.0
70	1246.0
71	1136.0
72	880.0
73	624.0
74	469.5
75	238.5
76	162.0
77	130.0
78	98.0
79	64.5
80	31.0
81	25.5
82	20.0
83	15.5
84	11.0
85	10.0
86	9.0
87	7.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	182451.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.89100087146686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.85327152790158	26.507391025535625
2	7.630593688714566	5.6300047684035714
3	3.790039816960837	4.19455086571189
4	2.617816604266952	3.8629549851741016
5	1.9804480893801628	3.6530356095609227
6	1.6342782432994591	3.617409605866781
7	1.338622452011648	3.4568185430608764
8	1.187080287632971	3.5034063940455247
9	1.0801093480715518	3.5861683410888405
>10	6.831283056991739	38.892634186713146
>50	0.03268556486598918	0.8369370406300871
>100	0.02079990491472039	1.3219987832349507
>500	0.001485707493908599	0.35790431403500117
>1k	0.001485707493908599	0.578785536938685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1056	0.578785536938685	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	653	0.35790431403500117	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	312	0.17100481773188417	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	252	0.13811927586036798	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	235	0.1288017056634384	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	214	0.11729176600840774	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	192	0.10523373398885179	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028500802955314028	0.0
2	0.0	0.0	0.0	0.2395163632975429	0.0
3	0.0	0.0	0.0	0.2828156600950392	0.0
4	0.0	0.0	0.0	0.3759913620643351	0.0
5	0.0	0.0	0.0	0.7623964790546502	0.0
6	0.0	0.0	0.0	0.9032562167376447	0.0
7	0.0	0.0	0.0	1.1164641465379745	0.0
8	0.0	0.0	0.0	1.5149272955478457	0.0
9	0.0	0.0	0.0	1.659623679782517	0.0
10	0.0	0.0	0.0	2.3551528903650842	0.0
11	0.0	0.0	0.0	2.7963672438079263	0.0
12	0.0	0.0	0.0	3.453529988873725	0.0
13	0.0	0.0	0.0	3.6037072967536488	0.0
14	0.0	0.0	0.0	3.679344043058136	0.0
15	0.0	0.0	0.0	3.8267808891154336	0.0
16	0.0	0.0	0.0	4.107952272116897	0.0
17	0.0	0.0	0.0	4.429682490093231	0.0
18	0.0	0.0	0.0	4.800192928512313	0.0
19	0.0	0.0	0.0	5.016689412499795	0.0
20	0.0	0.0	0.0	5.226608788112973	0.0
21	0.0	0.0	0.0	5.5160015565823155	0.0
22	0.0	0.0	0.0	5.7922401083030515	0.0
23	0.0	0.0	0.0	6.100268017166253	0.0
24	0.0	0.0	0.0	6.329370625537815	0.0
25	0.0	0.0	0.0	6.549703756076974	0.0
26	0.0	0.0	0.0	6.760719316419203	0.0
27	0.0	0.0	0.0	6.97776389277121	0.0
28	0.0	0.0	0.0	7.211251240058974	0.0
29	0.0	0.0	0.0	7.444190494982214	0.0
30	0.0	0.0	0.0	7.705082460496243	0.0
31	0.0	0.0	0.0	7.948983562709988	0.0
32	0.0	0.0	0.0	8.209875528224016	0.0
33	0.0	0.0	0.0	8.466382754821842	0.0
34	0.0	0.0	0.0	8.72837090506492	0.0
35	0.0	0.0	0.0	9.02982170555382	0.0
36	0.0	0.0	0.0	9.279203731412817	0.0
37	0.0	0.0	0.0	9.562019391507857	0.0
38	0.0	0.0	0.0	9.856344991257927	0.0
39	0.0	0.0	0.0	10.151766775737046	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAC	20	7.017675E-4	45.000004	39
ATAGGCC	20	7.017675E-4	45.000004	5
AAATAGG	20	7.017675E-4	45.000004	3
ATAGGAC	20	7.017675E-4	45.000004	5
CGCATAG	20	7.017675E-4	45.000004	2
CACGCCA	20	7.017675E-4	45.000004	27
GCGCATA	20	7.017675E-4	45.000004	1
TATGCCA	20	7.017675E-4	45.000004	10
ACGGGAT	40	6.7611836E-9	45.000004	5
AAACGGC	35	1.2044256E-7	45.000004	6
AACGGCT	35	1.2044256E-7	45.000004	7
TCAATCA	20	7.017675E-4	45.000004	13
ACAGGCG	20	7.017675E-4	45.000004	33
TCCAAGG	20	7.017675E-4	45.000004	3
TCATACT	20	7.017675E-4	45.000004	40
AATAGGC	20	7.017675E-4	45.000004	4
AGTAGCG	20	7.017675E-4	45.000004	1
CGTCATA	20	7.017675E-4	45.000004	38
AATCGGA	20	7.017675E-4	45.000004	32
TAGTACG	20	7.017675E-4	45.000004	1
>>END_MODULE