FastQCFastQC Report
Sat 14 Jan 2017
SRR2936156.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936156.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647190
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.22080069222330384No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.1324186096818554No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC7350.11356788578315487RNA PCR Primer, Index 29 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGTC1000.045.00000416
GCGTAAA253.888909E-545.0000041
ACGCGAA207.030999E-445.01
GGCGTAA207.030999E-445.01
GTCGTAA207.030999E-445.01
CGTAAAG302.1640262E-644.9999962
CGTTTTA3800.042.0394741
CGTTAAG603.6379788E-1241.2499962
GTAACGG556.002665E-1140.9090922
TCGTTGA403.4550976E-739.37524
GTAAGGT800.039.3754
CTTTCGT1150.039.13043215
ACTTTCG1150.039.13043214
TTACACG750.039.034
ATTCCGC356.2440595E-638.57142642
TAGTGCG356.2440595E-638.5714261
TCGGGTA950.037.89473727
GATGCGC301.139371E-437.49999611
CGTTATT1650.036.818181
AGGCGAT2600.036.3461537
Produced by FastQC (version 0.11.5)