##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936156.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	647190
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89161606328899	31.0	31.0	34.0	30.0	34.0
2	32.076768800506805	33.0	31.0	34.0	30.0	34.0
3	31.96594663081939	33.0	31.0	34.0	30.0	34.0
4	35.733392048702854	37.0	35.0	37.0	35.0	37.0
5	32.017762944421264	37.0	35.0	37.0	0.0	37.0
6	33.88747817487909	37.0	35.0	37.0	19.0	37.0
7	20.778990713700768	32.0	0.0	37.0	0.0	37.0
8	28.049364174353745	35.0	17.0	37.0	17.0	37.0
9	34.66436749640754	35.0	32.0	39.0	30.0	39.0
10	36.07958095767858	37.0	35.0	39.0	32.0	39.0
11	36.537274988797726	38.0	35.0	39.0	32.0	39.0
12	36.9724315888688	39.0	37.0	39.0	33.0	39.0
13	36.80604613791931	39.0	37.0	39.0	33.0	39.0
14	38.19512353404719	40.0	37.0	41.0	33.0	41.0
15	38.35252707860134	40.0	38.0	41.0	34.0	41.0
16	38.41201810905607	40.0	38.0	41.0	34.0	41.0
17	38.361152057355646	40.0	38.0	41.0	34.0	41.0
18	38.262199663159194	40.0	37.0	41.0	34.0	41.0
19	38.22914136497783	40.0	37.0	41.0	34.0	41.0
20	38.20599514825631	40.0	37.0	41.0	34.0	41.0
21	38.14843554443054	40.0	37.0	41.0	34.0	41.0
22	38.10984718552512	40.0	37.0	41.0	34.0	41.0
23	38.063982756223055	40.0	37.0	41.0	34.0	41.0
24	37.94922356649516	40.0	37.0	41.0	33.0	41.0
25	37.81528453777098	40.0	36.0	41.0	33.0	41.0
26	37.68442343052272	40.0	36.0	41.0	33.0	41.0
27	37.66043820207358	40.0	36.0	41.0	33.0	41.0
28	37.52247562539594	40.0	36.0	41.0	33.0	41.0
29	37.48022064617809	40.0	36.0	41.0	33.0	41.0
30	37.3426675319458	40.0	36.0	41.0	32.0	41.0
31	37.252531714025245	40.0	36.0	41.0	32.0	41.0
32	37.189913317572895	40.0	36.0	41.0	32.0	41.0
33	37.05680402972852	39.0	36.0	41.0	31.0	41.0
34	36.96808819666558	39.0	36.0	41.0	31.0	41.0
35	36.85808958729276	39.0	35.0	41.0	31.0	41.0
36	36.70978692501429	39.0	35.0	41.0	30.0	41.0
37	36.636511688993956	39.0	35.0	41.0	30.0	41.0
38	36.526275127860444	39.0	35.0	41.0	30.0	41.0
39	36.417285495758584	39.0	35.0	41.0	30.0	41.0
40	36.285284074228585	39.0	35.0	40.0	30.0	41.0
41	36.17640878258317	39.0	35.0	40.0	29.0	41.0
42	36.08939105981242	39.0	35.0	40.0	29.0	41.0
43	35.99424589378699	39.0	35.0	40.0	28.0	41.0
44	35.88283966068697	39.0	35.0	40.0	28.0	41.0
45	35.80210448245492	39.0	35.0	40.0	28.0	41.0
46	35.60037546933667	38.0	35.0	40.0	27.0	41.0
47	35.55575024335975	38.0	35.0	40.0	26.0	41.0
48	35.44469321219425	38.0	34.0	40.0	26.0	41.0
49	35.28656190608631	38.0	34.0	40.0	26.0	41.0
50	35.14054605293654	38.0	34.0	40.0	26.0	41.0
51	33.14674515984487	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	28.0
9	43.0
10	34.0
11	20.0
12	20.0
13	12.0
14	21.0
15	36.0
16	56.0
17	121.0
18	243.0
19	460.0
20	856.0
21	1358.0
22	1996.0
23	2851.0
24	4037.0
25	5430.0
26	6804.0
27	7762.0
28	8994.0
29	10632.0
30	13223.0
31	17216.0
32	22275.0
33	31526.0
34	43339.0
35	56020.0
36	75470.0
37	124044.0
38	128847.0
39	83398.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.89017135617052	20.733478576615834	24.517993170475442	12.858356896738208
2	31.991532625658614	25.66649670112332	24.721642794233535	17.620327878984533
3	31.868539377926115	24.893153478885644	26.290579273474556	16.947727869713685
4	28.31363278171789	25.65537168374048	27.097606576121386	18.933388958420245
5	22.469753858990405	37.35919899874843	22.66784097405708	17.503206168204084
6	26.551708153710656	35.83847092816638	24.214372904402108	13.395448013720854
7	48.54015049676293	43.00020086836941	5.488187394737249	2.97146124013041
8	82.33764427756918	5.790108005377092	7.921012376581839	3.9512353404718863
9	75.59325700335296	8.730048362922789	9.890758509865726	5.7859361238585265
10	38.527634852207235	30.36310820624546	17.330304856379115	13.77895208516819
11	28.45145938596085	27.103632627203755	26.316692161498167	18.128215825337225
12	27.8820748157419	23.165530987808836	29.763593380614655	19.188800815834608
13	24.577481110647568	24.68718614317279	29.80762990775506	20.927702838424576
14	19.001684204020457	28.81503113459726	29.654969947001653	22.528314714380627
15	19.471561674315115	27.41667825522644	33.28790617902007	19.82385389143837
16	21.990451026746396	25.63482130440829	32.08594075928244	20.28878690956288
17	21.89465226594972	25.59928305443533	29.12158716914662	23.384477510468333
18	22.12889568751062	27.457160957369553	28.88332638019747	21.530616974922356
19	24.391137069485005	27.309599962916607	27.165592793460963	21.133670174137425
20	25.94663081938843	25.670050526120612	28.344535607781328	20.03878304670962
21	25.30601523509325	26.111806424697537	28.01418439716312	20.56799394304609
22	23.527248566881443	26.040420896490986	27.876975849441433	22.555354687186142
23	23.261638776866146	26.70745839707041	28.60906379888441	21.421839027179036
24	22.161652683137874	25.374928537214732	29.38426119068589	23.07915758896151
25	22.072807058205473	27.86585083205859	27.36955144547969	22.691790664256246
26	21.11667361980253	28.698527480338075	27.648912993093216	22.535885906766172
27	22.35185957755837	28.525162626122157	27.6388695746226	21.484108221696875
28	20.872541293901328	28.542468208717686	28.936170212765955	21.648820284615027
29	21.998331247392574	26.76540119593937	28.939569523632937	22.29669803303512
30	21.52165515536396	27.610129946383598	28.417157249030424	22.451057649222022
31	22.31199493193653	28.121417203603272	26.87819651107094	22.688391353389267
32	22.875816993464053	28.380382886014928	26.885458675195846	21.858341445325173
33	22.114989415782073	29.40759282436379	26.809592237210094	21.667825522644048
34	21.813686861663502	28.19898329702251	27.56485730620065	22.422472535113336
35	21.665816838949922	26.813918632858975	27.222917535808648	24.297346992382455
36	21.559665631421993	29.005083514887435	26.480322625504105	22.954928228186468
37	21.6180719726819	29.219703641898054	27.517112439932635	21.645111945487415
38	21.254036681654537	28.290610176300625	27.071648202228094	23.383704939816745
39	22.584712371946413	26.781934207883314	27.65339390287242	22.979959517297857
40	22.402540212302416	27.1057958250282	28.37451134906287	22.117152613606514
41	19.813192416446483	27.81161637231725	28.880699639982076	23.49449157125419
42	21.46046755975834	28.18955793507316	27.14952332390797	23.200451181260526
43	22.112517189696998	27.457006443239234	27.073811400052534	23.356664967011234
44	21.63321435745299	27.883465442914755	26.679027797092044	23.804292402540213
45	21.23225018927981	27.232960954279267	26.817472457856272	24.71731639858465
46	20.75464701246929	29.629938657890264	26.685362876435047	22.930051453205397
47	21.816777144269846	27.567947588806994	27.982818028708728	22.632457238214435
48	21.11837327523602	26.154915867056044	28.230040637216273	24.496670220491666
49	19.771473601260837	27.59715075943695	28.650319071679103	23.98105656762311
50	19.49813810472968	26.847911741528762	29.135338926744854	24.518611226996708
51	19.686027287195415	27.20962932060137	27.351782320493207	25.752561071710005
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	277.0
1	850.0
2	1423.0
3	3211.5
4	5000.0
5	3631.0
6	2262.0
7	1959.0
8	1656.0
9	1671.5
10	1687.0
11	1667.5
12	1648.0
13	1645.5
14	1643.0
15	1650.5
16	1658.0
17	1691.0
18	1724.0
19	1883.0
20	2042.0
21	2305.0
22	2568.0
23	2839.5
24	3111.0
25	4199.0
26	6706.0
27	8125.0
28	9697.5
29	11270.0
30	12907.5
31	14545.0
32	17186.5
33	19828.0
34	21784.0
35	23740.0
36	24953.0
37	26166.0
38	28075.5
39	29985.0
40	32768.5
41	35552.0
42	38387.0
43	41222.0
44	43652.5
45	46083.0
46	49588.0
47	53093.0
48	51353.5
49	49614.0
50	46556.5
51	43499.0
52	39645.0
53	35791.0
54	34132.5
55	32474.0
56	31079.5
57	29685.0
58	29171.5
59	28658.0
60	28098.0
61	27538.0
62	24654.5
63	21771.0
64	17596.0
65	13421.0
66	10871.0
67	8321.0
68	6969.5
69	5618.0
70	4600.5
71	3583.0
72	3007.0
73	2431.0
74	1966.5
75	1144.5
76	787.0
77	604.0
78	421.0
79	332.5
80	244.0
81	177.0
82	110.0
83	83.0
84	56.0
85	43.5
86	31.0
87	25.5
88	20.0
89	14.5
90	9.0
91	5.5
92	2.0
93	3.5
94	5.0
95	3.0
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	647190.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.43286803191959
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.02412867712893	29.21825304689769
2	6.963420679256272	5.352484560331923
3	2.9499427689710167	3.4012428342473555
4	1.89377535858895	2.9113287375500128
5	1.4530095092346949	2.7921661358770633
6	1.2144024990337452	2.800378258979837
7	1.028747503935499	2.7676401939843798
8	0.9264455211427288	2.848476676227317
9	0.848229747421304	2.933991174905231
>10	6.625985131942187	41.09693454047167
>50	0.04449592332161746	1.1587263338820941
>100	0.024719957397886082	1.9123821170535078
>500	0.00224726885435328	0.5591528238553161
>1k	4.49453770870656E-4	0.2468425657365606
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1429	0.22080069222330384	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	857	0.1324186096818554	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	735	0.11356788578315487	RNA PCR Primer, Index 29 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.545141303172175E-4	0.0	0.0	0.05531605865356387	0.0
2	1.545141303172175E-4	0.0	0.0	0.2646827052333936	0.0
3	1.545141303172175E-4	0.0	0.0	0.3493564486472288	0.0
4	1.545141303172175E-4	0.0	0.0	0.5168497659110926	0.0
5	1.545141303172175E-4	0.0	0.0	0.919977131908713	0.0
6	1.545141303172175E-4	0.0	0.0	1.1953213121339947	0.0
7	1.545141303172175E-4	0.0	0.0	1.4173581173998362	0.0
8	1.545141303172175E-4	0.0	0.0	1.9334353126593427	0.0
9	1.545141303172175E-4	0.0	0.0	2.097683833186545	0.0
10	1.545141303172175E-4	0.0	0.0	2.691790664256246	0.0
11	1.545141303172175E-4	0.0	0.0	3.229345323629846	0.0
12	1.545141303172175E-4	0.0	0.0	3.7923948145057866	0.0
13	1.545141303172175E-4	0.0	0.0	4.075310187116612	0.0
14	1.545141303172175E-4	0.0	0.0	4.197376350067214	0.0
15	3.09028260634435E-4	0.0	0.0	4.377539826017089	0.0
16	3.09028260634435E-4	0.0	0.0	4.727205302924952	0.0
17	3.09028260634435E-4	0.0	0.0	5.109318747199431	0.0
18	0.0038628532579304377	0.0	0.0	5.552774301209846	1.545141303172175E-4
19	0.0038628532579304377	0.0	0.0	5.807259073842303	1.545141303172175E-4
20	0.0038628532579304377	0.0	0.0	6.0690060105996695	1.545141303172175E-4
21	0.0038628532579304377	0.0	0.0	6.396266938611536	1.545141303172175E-4
22	0.0038628532579304377	0.0	0.0	6.788269287226317	1.545141303172175E-4
23	0.0038628532579304377	0.0	0.0	7.163275081506204	1.545141303172175E-4
24	0.0038628532579304377	0.0	0.0	7.464423121494461	1.545141303172175E-4
25	0.0038628532579304377	0.0	0.0	7.739458273459108	1.545141303172175E-4
26	0.0038628532579304377	0.0	0.0	7.99255241891871	1.545141303172175E-4
27	0.0038628532579304377	0.0	0.0	8.24240176764165	1.545141303172175E-4
28	0.0038628532579304377	0.0	0.0	8.515737264172808	1.545141303172175E-4
29	0.0038628532579304377	0.0	0.0	8.786909562879526	1.545141303172175E-4
30	0.0038628532579304377	0.0	0.0	9.090838857213493	1.545141303172175E-4
31	0.0038628532579304377	0.0	0.0	9.39971260371761	1.545141303172175E-4
32	0.0038628532579304377	0.0	0.0	9.681855405676849	1.545141303172175E-4
33	0.0038628532579304377	0.0	0.0	9.97064231523973	1.545141303172175E-4
34	0.0038628532579304377	0.0	0.0	10.246450037855961	1.545141303172175E-4
35	0.0038628532579304377	0.0	0.0	10.55424218544786	1.545141303172175E-4
36	0.0038628532579304377	0.0	0.0	10.86079821999722	1.545141303172175E-4
37	0.0038628532579304377	0.0	0.0	11.154220553469615	1.545141303172175E-4
38	0.0038628532579304377	0.0	0.0	11.450887683678673	1.545141303172175E-4
39	0.0038628532579304377	0.0	0.0	11.744464531281386	1.545141303172175E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGTC	100	0.0	45.000004	16
GCGTAAA	25	3.888909E-5	45.000004	1
ACGCGAA	20	7.030999E-4	45.0	1
GGCGTAA	20	7.030999E-4	45.0	1
GTCGTAA	20	7.030999E-4	45.0	1
CGTAAAG	30	2.1640262E-6	44.999996	2
CGTTTTA	380	0.0	42.039474	1
CGTTAAG	60	3.6379788E-12	41.249996	2
GTAACGG	55	6.002665E-11	40.909092	2
TCGTTGA	40	3.4550976E-7	39.375	24
GTAAGGT	80	0.0	39.375	4
CTTTCGT	115	0.0	39.130432	15
ACTTTCG	115	0.0	39.130432	14
TTACACG	75	0.0	39.0	34
ATTCCGC	35	6.2440595E-6	38.571426	42
TAGTGCG	35	6.2440595E-6	38.571426	1
TCGGGTA	95	0.0	37.894737	27
GATGCGC	30	1.139371E-4	37.499996	11
CGTTATT	165	0.0	36.81818	1
AGGCGAT	260	0.0	36.346153	7
>>END_MODULE