FastQCFastQC Report
Sat 14 Jan 2017
SRR2936153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873099
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185202.1211798432938305No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110301.2633160729768331No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18170.20810927512229424No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTT11650.13343274932166915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG453.8380676E-1045.09
CGTTTTA42100.044.6258931
CGTTATT16950.043.805311
CGTTTTT76000.043.7269741
GTTTTAT45500.041.8351672
CGGTTTA2600.041.5384641
GTTATTT18750.040.322
TTACGGG1950.038.0769273
ATGGGCG1450.037.241385
CGGTTTT7350.037.0408171
CGTTCTA1300.036.3461571
AGGCGAT4850.036.185577
TACGGGT504.8767106E-836.04
TACGATC250.002106831436.011
TATCGCA250.002106831436.01
ACGGGAT1700.035.7352945
CTATCTC4350.035.6896555
TAGACGG950.035.5263182
GCGATAC458.696734E-735.09
TTTTATT56200.034.630783
Produced by FastQC (version 0.11.5)