##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936153.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	873099
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.604776777891168	31.0	31.0	34.0	30.0	34.0
2	31.802193107539924	31.0	31.0	34.0	30.0	34.0
3	30.94553767671249	31.0	31.0	34.0	27.0	34.0
4	35.17101840684733	37.0	35.0	37.0	32.0	37.0
5	31.742163259836513	35.0	35.0	37.0	0.0	37.0
6	33.691369478146235	37.0	35.0	37.0	17.0	37.0
7	21.14023266548238	32.0	0.0	37.0	0.0	37.0
8	28.24675781326058	35.0	17.0	37.0	17.0	37.0
9	34.74029176530955	37.0	32.0	39.0	30.0	39.0
10	36.20308235377661	37.0	35.0	39.0	32.0	39.0
11	36.66459702737032	38.0	35.0	39.0	33.0	39.0
12	37.01540375146461	39.0	37.0	39.0	34.0	39.0
13	36.85399479325941	39.0	37.0	39.0	33.0	39.0
14	38.23601905396753	40.0	38.0	41.0	34.0	41.0
15	38.36194749965353	40.0	38.0	41.0	34.0	41.0
16	38.39016995781692	40.0	38.0	41.0	34.0	41.0
17	38.30556901336504	40.0	38.0	41.0	33.0	41.0
18	38.08442112521031	40.0	37.0	41.0	33.0	41.0
19	37.91919816653094	40.0	37.0	41.0	34.0	41.0
20	37.72250111384849	39.0	35.0	41.0	33.0	41.0
21	37.59093069628988	39.0	35.0	41.0	33.0	41.0
22	37.460911076521676	39.0	35.0	40.0	33.0	41.0
23	37.32944259471148	39.0	35.0	40.0	33.0	41.0
24	37.109789382418256	39.0	35.0	40.0	33.0	41.0
25	36.93231695374752	38.0	35.0	40.0	32.0	41.0
26	36.790197904246824	38.0	35.0	40.0	32.0	41.0
27	36.7218929353945	38.0	35.0	40.0	32.0	41.0
28	36.57380319986623	38.0	35.0	40.0	32.0	41.0
29	36.48996963689112	38.0	35.0	40.0	31.0	41.0
30	36.250728726066576	38.0	35.0	40.0	31.0	41.0
31	36.00112243857799	38.0	35.0	40.0	30.0	41.0
32	35.64471726573962	38.0	34.0	40.0	28.0	41.0
33	35.14413371221362	38.0	34.0	40.0	25.0	41.0
34	34.60305417827761	38.0	33.0	40.0	21.0	41.0
35	34.12371792889466	38.0	33.0	40.0	18.0	41.0
36	33.77666793800016	38.0	33.0	40.0	16.0	41.0
37	33.60946925835444	38.0	33.0	40.0	15.0	41.0
38	33.385210611855015	38.0	33.0	40.0	15.0	41.0
39	33.20082945920222	38.0	33.0	40.0	12.0	41.0
40	33.04287028160609	37.0	32.0	40.0	11.0	41.0
41	32.83886363402088	37.0	32.0	40.0	10.0	41.0
42	32.67306456656118	37.0	31.0	40.0	10.0	41.0
43	32.53984141546377	37.0	31.0	40.0	10.0	41.0
44	32.35325547274708	37.0	31.0	40.0	10.0	41.0
45	32.26505127139076	36.0	31.0	40.0	10.0	41.0
46	32.134117665923334	36.0	30.0	40.0	10.0	41.0
47	32.07753645348351	36.0	30.0	40.0	10.0	41.0
48	31.93430069213228	36.0	30.0	40.0	10.0	41.0
49	31.790730489898625	35.0	29.0	40.0	10.0	41.0
50	31.62054818525734	35.0	29.0	40.0	10.0	41.0
51	29.894729005530873	34.0	24.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	17.0
9	50.0
10	42.0
11	42.0
12	26.0
13	23.0
14	41.0
15	61.0
16	92.0
17	230.0
18	426.0
19	916.0
20	1769.0
21	3050.0
22	5386.0
23	9145.0
24	14884.0
25	22266.0
26	27524.0
27	28289.0
28	26013.0
29	24256.0
30	24630.0
31	27995.0
32	35032.0
33	47733.0
34	61696.0
35	76897.0
36	91310.0
37	137335.0
38	127675.0
39	78238.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.6744172195822	17.301588937795138	21.12704286684557	27.89695097577709
2	43.4917460677426	20.378101452412615	21.867852328315575	14.262300151529208
3	27.391853615683903	20.22645770983588	38.125344319487255	14.256344354992962
4	23.79237635136451	21.532266100407856	38.871651439298404	15.803706108929227
5	19.50214122338933	32.68277709629721	32.948611784001585	14.866469896311873
6	21.70246443988597	28.860186530966132	36.49460141404354	12.942747615104357
7	40.62597712286923	41.33906922353594	15.091186681006391	2.9437669725884463
8	70.43015740483038	3.8276300854771335	22.082031934522888	3.660180575169597
9	64.97659486495803	5.646896858202792	24.031295420107	5.345212856732169
10	35.02833012063924	20.444073352506418	30.70946135547057	13.818135171383773
11	25.711631785169835	22.30354175185174	35.88287238904179	16.101954073936632
12	23.404104231020767	19.110662135679917	39.45566310349685	18.029570529802463
13	21.16919158079439	18.64450652216988	40.957096503374764	19.22920539366097
14	18.168042799270186	20.75503465242773	41.519690206952475	19.557232341349607
15	18.327474891163543	21.745987568420073	42.12993028282016	17.796607257596218
16	22.005980994136976	21.33045622546813	39.47272875126417	17.190834029130716
17	21.500998168592563	22.012623997965868	38.51430364712364	17.972074186317933
18	21.532724238602956	22.022359434611655	38.71496817657562	17.729948150209772
19	21.096233073225374	23.913095765772265	36.78987148078282	18.20079968021954
20	21.90667954034995	23.484278415162542	36.49838105415308	18.11066099033443
21	22.033583820391502	23.308124279148185	37.84839978055181	16.80989211990851
22	21.180301432025463	20.795007209949844	38.83202248542261	19.192668872602077
23	20.874837790445298	21.107915597200318	38.63204516326327	19.38520144909111
24	21.141932358186185	20.935312032197952	39.02604401104571	18.89671159857015
25	21.137350976235226	22.192214170443446	36.93051990667725	19.73991494664408
26	19.745298070436455	22.244900062879466	37.73707219914351	20.272729667540563
27	19.574412523665703	21.969902611273177	37.52380886932639	20.931875995734732
28	19.136432409154057	22.89053131431831	37.40767083686959	20.565365439658045
29	20.254747743383053	21.60831704079377	36.986527301027714	21.15040791479546
30	20.369282292157017	21.171253202672318	37.51224087990022	20.947223625270446
31	20.721361495088185	21.364587521002772	36.1894813761097	21.724569607799342
32	21.42838326466987	21.92202716988566	35.53995595001254	21.109633615431928
33	20.754003841488768	22.774393281861506	35.92490656844184	20.546696308207892
34	19.89785808940338	22.633057648674434	37.08789037669268	20.38119388522951
35	20.34293934593901	24.217757665511012	35.54682802293898	19.892474965611
36	20.59732057876598	25.07447609034027	34.62081619610147	19.707387134792278
37	21.11604755016327	25.259563921159	34.26358293847548	19.360805590202258
38	20.933708548515117	25.309730053521996	34.09040670072924	19.666154697233647
39	21.4775185860939	24.761796772187346	33.44901322759504	20.311671414123715
40	21.99280952102797	24.00082923013312	34.00072614903923	20.00563509979968
41	20.32816438914716	25.063595308206743	33.77623843344226	20.832001869203836
42	20.25875645259014	25.444880821075273	33.106096788565786	21.1902659377688
43	20.471218040565848	24.825019843110574	33.510518280286654	21.19324383603692
44	20.827649556350426	24.507530073909145	32.857900421372605	21.806919948367824
45	20.781148529548197	24.913784118410398	32.2855712811491	22.019496070892306
46	20.755149186976503	24.934973009933582	32.47329340658963	21.83658439650028
47	20.198396745386262	24.56777524656425	33.73431878859098	21.499509219458503
48	20.04228615540735	23.822957075887157	34.364831479591665	21.769925289113836
49	20.442928007018676	23.581747316169185	34.403887760723585	21.571436916088555
50	19.49343659768251	23.680017959017245	35.02684117150518	21.799704271795065
51	19.159568388006402	23.452666879700928	34.95537161307023	22.43239311922245
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	413.0
1	985.0
2	1557.0
3	27579.0
4	53601.0
5	36101.5
6	18602.0
7	16146.5
8	13691.0
9	13230.0
10	12769.0
11	12124.0
12	11479.0
13	10919.0
14	10359.0
15	9606.5
16	8854.0
17	8143.5
18	7433.0
19	7102.5
20	6772.0
21	6579.5
22	6387.0
23	6441.5
24	6496.0
25	6768.0
26	7700.5
27	8361.0
28	9292.5
29	10224.0
30	11529.0
31	12834.0
32	14586.5
33	16339.0
34	18287.0
35	20235.0
36	21549.5
37	22864.0
38	24548.5
39	26233.0
40	28297.0
41	30361.0
42	32970.5
43	35580.0
44	40559.5
45	45539.0
46	52779.5
47	60020.0
48	60328.0
49	60636.0
50	59179.0
51	57722.0
52	53343.0
53	48964.0
54	46440.5
55	43917.0
56	42752.0
57	41587.0
58	40433.0
59	39279.0
60	37189.0
61	35099.0
62	31805.5
63	28512.0
64	24904.0
65	21296.0
66	17901.5
67	14507.0
68	12305.5
69	10104.0
70	8485.0
71	6866.0
72	5667.5
73	4469.0
74	3650.0
75	2159.5
76	1488.0
77	1158.5
78	829.0
79	645.5
80	462.0
81	344.0
82	226.0
83	185.5
84	145.0
85	104.0
86	63.0
87	45.5
88	28.0
89	21.0
90	14.0
91	10.0
92	6.0
93	4.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	873099.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.058950547658085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.80105206490806	34.506832104572396
2	9.646304037786525	9.271824974393141
3	5.262475553249757	7.587271571156691
4	3.7200145832820546	7.151199875780767
5	2.630120758817462	6.320042174118868
6	1.9457842901852824	5.610741058705469
7	1.4519114040863534	4.884413685799681
8	1.0217649187184126	3.9283959760016063
9	0.7359926070876188	3.183392907671924
>10	1.7485428992957508	11.147952023310854
>50	0.018018696151895663	0.5993129619617544
>100	0.015515660541967527	1.3738301377058117
>500	0.001501515533286772	0.5222069903721015
>1k	5.005051777622574E-4	0.35864146598102414
>5k	0.0	0.0
>10k+	5.005051777622574E-4	3.553942092467895
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18520	2.1211798432938305	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11030	1.2633160729768331	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1817	0.20810927512229424	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTT	1165	0.13343274932166915	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1453454877396492E-4	0.0	0.0	0.04512661221694218	0.0
2	1.1453454877396492E-4	0.0	0.0	0.18417155442853558	0.0
3	1.1453454877396492E-4	0.0	0.0	0.31829151104284853	0.0
4	1.1453454877396492E-4	0.0	0.0	0.4826485885334882	0.0
5	1.1453454877396492E-4	0.0	0.0	0.9348309870931018	0.0
6	1.1453454877396492E-4	0.0	0.0	1.6451742585892322	0.0
7	1.1453454877396492E-4	0.0	0.0	2.2094859803985574	0.0
8	1.1453454877396492E-4	0.0	0.0	3.1889854415135055	0.0
9	1.1453454877396492E-4	0.0	0.0	3.8153748887583196	0.0
10	1.1453454877396492E-4	0.0	0.0	4.625935890431669	0.0
11	2.2906909754792984E-4	0.0	0.0	5.193798183252987	0.0
12	2.2906909754792984E-4	0.0	0.0	5.632121901410951	0.0
13	3.4360364632189476E-4	0.0	0.0	5.897956589115323	0.0
14	3.4360364632189476E-4	0.0	0.0	6.081211867153668	0.0
15	4.581381950958597E-4	0.0	0.0	6.224036449474802	0.0
16	4.581381950958597E-4	0.0	0.0	6.388278992416668	0.0
17	4.581381950958597E-4	0.0	0.0	6.536028560335082	0.0
18	4.581381950958597E-4	0.0	0.0	6.743794231811055	0.0
19	4.581381950958597E-4	0.0	0.0	6.879861275754525	0.0
20	4.581381950958597E-4	0.0	0.0	7.020051563453858	0.0
21	4.581381950958597E-4	0.0	0.0	7.172497047872005	0.0
22	4.581381950958597E-4	0.0	0.0	7.35117094395939	0.0
23	4.581381950958597E-4	0.0	0.0	7.538320396656049	0.0
24	4.581381950958597E-4	0.0	0.0	7.71481813631673	0.0
25	4.581381950958597E-4	0.0	0.0	7.870126984454226	0.0
26	4.581381950958597E-4	0.0	0.0	8.028299196311071	0.0
27	4.581381950958597E-4	0.0	0.0	8.190938255570101	0.0
28	4.581381950958597E-4	0.0	0.0	8.357127885841125	0.0
29	4.581381950958597E-4	0.0	0.0	8.52102682513667	0.0
30	4.581381950958597E-4	0.0	0.0	8.745629075282414	0.0
31	4.581381950958597E-4	0.0	0.0	8.926020989601408	0.0
32	4.581381950958597E-4	0.0	0.0	9.119355307931862	0.0
33	4.581381950958597E-4	0.0	0.0	9.30192337867756	0.0
34	4.581381950958597E-4	0.0	0.0	9.495830369751884	0.0
35	5.726727438698247E-4	0.0	0.0	9.702336161191342	0.0
36	5.726727438698247E-4	0.0	0.0	9.900022792375205	0.0
37	5.726727438698247E-4	0.0	0.0	10.106643118363438	0.0
38	5.726727438698247E-4	0.0	0.0	10.40534922156594	0.0
39	5.726727438698247E-4	0.0	0.0	10.731314547376643	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	45	3.8380676E-10	45.0	9
CGTTTTA	4210	0.0	44.625893	1
CGTTATT	1695	0.0	43.80531	1
CGTTTTT	7600	0.0	43.726974	1
GTTTTAT	4550	0.0	41.835167	2
CGGTTTA	260	0.0	41.538464	1
GTTATTT	1875	0.0	40.32	2
TTACGGG	195	0.0	38.076927	3
ATGGGCG	145	0.0	37.24138	5
CGGTTTT	735	0.0	37.040817	1
CGTTCTA	130	0.0	36.346157	1
AGGCGAT	485	0.0	36.18557	7
TACGGGT	50	4.8767106E-8	36.0	4
TACGATC	25	0.0021068314	36.0	11
TATCGCA	25	0.0021068314	36.0	1
ACGGGAT	170	0.0	35.735294	5
CTATCTC	435	0.0	35.689655	5
TAGACGG	95	0.0	35.526318	2
GCGATAC	45	8.696734E-7	35.0	9
TTTTATT	5620	0.0	34.63078	3
>>END_MODULE