FastQCFastQC Report
Sat 14 Jan 2017
SRR2936152.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936152.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595804
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35170.5902947949325617No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21470.3603534048109781No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8030.13477586588878224No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATA207.030547E-445.0000049
TACGAAT502.1827873E-1145.012
CGTTTTA7250.041.275861
CGTTATT3300.040.909091
CATAGTT1050.040.71428736
GTTAGCG501.0804797E-940.51
TCTACGG501.0804797E-940.52
CGTTTTT12250.040.2244871
CGTTAGG451.9255822E-840.0000043
TCACGAC403.4544792E-739.37500425
ATAGGCG700.038.571435
CGTTAAG356.2432227E-638.571432
GTTAACG356.2432227E-638.571431
GCTAACG356.2432227E-638.571431
TGCACGG356.2432227E-638.571432
CGGTTTA659.094947E-1238.0769271
GCTACGA659.094947E-1238.07692710
AGGCGAT3500.037.9285747
TAGGCAT850.037.0588236
ATAGGCC552.744855E-936.818185
Produced by FastQC (version 0.11.5)