##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936152.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595804
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97974501681761	33.0	31.0	34.0	30.0	34.0
2	32.164624608092595	33.0	31.0	34.0	30.0	34.0
3	31.94200273915583	33.0	31.0	34.0	30.0	34.0
4	35.750347429691644	37.0	35.0	37.0	35.0	37.0
5	32.10858940188384	37.0	35.0	37.0	0.0	37.0
6	33.97975005203053	37.0	35.0	37.0	19.0	37.0
7	20.71669206651852	32.0	0.0	37.0	0.0	37.0
8	28.080330108559192	35.0	17.0	37.0	17.0	37.0
9	34.812871346953024	37.0	32.0	39.0	32.0	39.0
10	36.20490463306725	37.0	35.0	39.0	32.0	39.0
11	36.68169733670805	38.0	35.0	39.0	33.0	39.0
12	37.07714281877933	39.0	37.0	39.0	34.0	39.0
13	36.92767420158307	39.0	37.0	39.0	33.0	39.0
14	38.322928345563305	40.0	38.0	41.0	34.0	41.0
15	38.485127659431626	40.0	38.0	41.0	34.0	41.0
16	38.537836268302996	40.0	38.0	41.0	34.0	41.0
17	38.4856177534894	40.0	38.0	41.0	34.0	41.0
18	38.374623198232975	40.0	38.0	41.0	34.0	41.0
19	38.31764808561205	40.0	37.0	41.0	34.0	41.0
20	38.267242247450504	40.0	37.0	41.0	34.0	41.0
21	38.188817127780275	40.0	37.0	41.0	34.0	41.0
22	38.15668407731402	40.0	37.0	41.0	34.0	41.0
23	38.09781068942135	40.0	37.0	41.0	34.0	41.0
24	37.98094675430175	40.0	36.0	41.0	33.0	41.0
25	37.82603675034072	40.0	36.0	41.0	33.0	41.0
26	37.680453639116216	40.0	36.0	41.0	33.0	41.0
27	37.656794851998306	40.0	36.0	41.0	33.0	41.0
28	37.52338856402441	40.0	36.0	41.0	33.0	41.0
29	37.47679606044941	40.0	36.0	41.0	33.0	41.0
30	37.327985377741676	40.0	36.0	41.0	33.0	41.0
31	37.202712637041714	40.0	35.0	41.0	32.0	41.0
32	37.07800719699767	39.0	35.0	41.0	31.0	41.0
33	36.90311411135205	39.0	35.0	41.0	31.0	41.0
34	36.698244724775265	39.0	35.0	41.0	30.0	41.0
35	36.52533047780814	39.0	35.0	41.0	30.0	41.0
36	36.36146954367544	39.0	35.0	41.0	30.0	41.0
37	36.28862847513612	39.0	35.0	41.0	30.0	41.0
38	36.15782203543447	39.0	35.0	41.0	29.0	41.0
39	36.09246497170211	39.0	35.0	41.0	29.0	41.0
40	35.98017804512894	39.0	35.0	41.0	28.0	41.0
41	35.82187430765822	39.0	35.0	40.0	27.0	41.0
42	35.71659304066438	39.0	35.0	40.0	27.0	41.0
43	35.62258729380803	39.0	35.0	40.0	26.0	41.0
44	35.49825445952025	38.0	35.0	40.0	26.0	41.0
45	35.41090526414727	38.0	35.0	40.0	26.0	41.0
46	35.2687041376023	38.0	34.0	40.0	25.0	41.0
47	35.22164168082121	38.0	34.0	40.0	25.0	41.0
48	35.11030808789468	38.0	34.0	40.0	24.0	41.0
49	34.978638948379	38.0	34.0	40.0	24.0	41.0
50	34.830471094521016	38.0	34.0	40.0	24.0	41.0
51	32.93767245604259	36.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	10.0
9	29.0
10	27.0
11	21.0
12	23.0
13	10.0
14	27.0
15	35.0
16	69.0
17	119.0
18	198.0
19	424.0
20	747.0
21	1270.0
22	1953.0
23	3138.0
24	4546.0
25	6239.0
26	7516.0
27	8217.0
28	8710.0
29	10098.0
30	11654.0
31	14840.0
32	19946.0
33	28840.0
34	39890.0
35	52083.0
36	68040.0
37	113439.0
38	116549.0
39	77074.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.455586065216075	20.38170270760183	24.96542487126639	14.197286355915702
2	34.37472725930004	23.974494968143887	25.5985189760391	16.052258796516973
3	31.16561822344261	23.468120388584165	29.46287705352767	15.903384334445555
4	26.927983027975642	25.246389752334657	29.743170572872955	18.082456646816738
5	22.232982658726698	36.71929023638646	24.590637189411282	16.45708991547556
6	25.84155191975885	33.63505448100382	27.249733133715115	13.27366046552222
7	47.548858349390066	43.20934401246047	6.694147739860759	2.5476498982886993
8	82.64613866305027	4.74854146665682	9.757235600969446	2.848084269323469
9	76.71096535102147	7.098139656665616	11.428254929473452	4.762640062839457
10	39.59506817678297	25.65709528636934	19.966465481937014	14.781371054910675
11	28.312163060335276	27.05990560654175	27.094984256567596	17.532947076555377
12	25.942424018637	23.664829373418105	31.318520855851922	19.07422575209297
13	23.33351236312613	24.144349484058516	32.786117582292164	19.73602057052319
14	19.889762405086238	26.086095427355303	33.29920577908172	20.72493638847675
15	20.177440903384333	25.90852025162637	34.61138226665145	19.302656578337842
16	23.365905566260047	24.912890816442992	32.2637981618116	19.45740545548536
17	23.456371558431968	24.649213499741528	30.620472504380636	21.27394243744587
18	24.221556082201527	24.930346221240544	31.148498499506548	19.69959919705138
19	24.075702747883533	26.546313888459967	29.453981510698153	19.924001852958355
20	24.499331995085633	25.618995508590075	30.513558149995635	19.368114346328657
21	24.618163020053572	25.64433941363267	30.92610992876852	18.81138763754523
22	24.221556082201527	23.936395190364617	30.99458882451276	20.847459902921095
23	22.815892474706448	25.026350947627073	30.711442017844792	21.446314559821687
24	22.126571825633935	24.412894173251605	32.38531463367148	21.075219367442983
25	21.69018670569516	26.88686212244295	30.472269404032197	20.95068176782969
26	20.917281522111296	27.349766030439543	30.752562923377486	20.980389524071676
27	21.380185430107886	26.58659559183893	31.38548918771945	20.64772979033373
28	20.517485616075085	26.544299803291015	31.746178273391923	21.192036307241978
29	20.896804989560323	25.82241811065384	32.313814610173814	20.96696228961202
30	21.211337956777733	25.650381669139517	32.13607159401414	21.002208780068614
31	23.113977079710775	26.206604856630705	30.161261085860453	20.518156977798068
32	22.90367301998644	26.279615444005078	30.391705997274272	20.425005538734215
33	22.905351424293897	26.049674053883493	29.855287980611074	21.18968654121154
34	21.63580640613356	25.91003081550308	31.277567790749977	21.176594987613377
35	21.51798242375009	25.975992104786137	31.177702734456297	21.328322737007472
36	23.138985303891886	26.702069808191958	29.355962699142673	20.802982188773488
37	22.048693865767937	27.430329437197464	30.127860840142063	20.393115856892535
38	21.446146719390942	27.709112392666047	30.000973474498327	20.843767413444688
39	21.974172714516854	26.93906049640486	29.82944055427624	21.257326234802047
40	23.19118367785379	26.187806728387187	29.618129451967423	21.002880141791596
41	20.964444683150834	26.370920638330723	30.685594591509957	21.97904008700848
42	21.26403985203188	26.293210518895478	29.96035609025787	22.482393538814776
43	21.195225275426147	26.12520224771905	30.321884378084068	22.357688098770737
44	21.526374445287377	26.325435881598647	29.939879557706895	22.20831011540708
45	21.329833300884182	26.561923048519308	29.290504931151855	22.817738719444648
46	21.531241817779	27.410692106800223	28.945928526830972	22.112137548589807
47	20.412921027720525	26.993776476827954	30.775053541097407	21.818248954354118
48	20.861054977811495	26.399117830696	30.990392813744116	21.749434377748386
49	21.29341192741237	26.038260904592786	30.63087861108687	22.037448556907975
50	20.48123208303402	26.43016831038395	30.99677075011245	22.09182885646958
51	21.038965834401917	26.370081436176996	29.620647058428613	22.970305670992474
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	502.0
1	1037.5
2	1573.0
3	5872.5
4	10172.0
5	6829.5
6	3487.0
7	3041.5
8	2596.0
9	2528.5
10	2461.0
11	2449.0
12	2437.0
13	2336.5
14	2236.0
15	2264.5
16	2293.0
17	2267.0
18	2241.0
19	2501.5
20	2762.0
21	3095.0
22	3428.0
23	3781.0
24	4134.0
25	4905.0
26	6903.0
27	8130.0
28	9799.0
29	11468.0
30	13399.0
31	15330.0
32	17003.5
33	18677.0
34	20600.5
35	22524.0
36	24005.0
37	25486.0
38	27008.5
39	28531.0
40	30055.5
41	31580.0
42	33756.5
43	35933.0
44	37122.0
45	38311.0
46	39601.0
47	40891.0
48	41044.5
49	41198.0
50	39633.5
51	38069.0
52	35963.5
53	33858.0
54	32076.0
55	30294.0
56	29280.5
57	28267.0
58	26857.0
59	25447.0
60	23270.5
61	21094.0
62	19274.5
63	17455.0
64	15145.0
65	12835.0
66	10789.5
67	8744.0
68	7323.0
69	5902.0
70	5037.0
71	4172.0
72	3346.0
73	2520.0
74	1970.0
75	1111.5
76	803.0
77	617.5
78	432.0
79	318.0
80	204.0
81	151.0
82	98.0
83	73.0
84	48.0
85	36.5
86	25.0
87	25.0
88	25.0
89	22.0
90	19.0
91	14.0
92	9.0
93	7.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	595804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.69228008982821
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.10748932434556	27.555879642773583
2	7.426898906097044	5.59873507534897
3	3.5636045780261485	4.02961145653067
4	2.398254856421851	3.615827751001725
5	1.7755966285549498	3.346314272502391
6	1.4815259732577988	3.3505255166632963
7	1.2421486461972953	3.277359027996856
8	1.1093320210055915	3.345060259868635
9	1.0037105633354995	3.4048925714124807
>10	6.853657387256021	40.04248371642241
>50	0.025671783610690665	0.6320619505054027
>100	0.010656212064559141	0.6205599565934831
>500	4.843732756617792E-4	0.14660477938939892
>1k	9.687465513235584E-4	1.034084022990729
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3517	0.5902947949325617	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2147	0.3603534048109781	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	803	0.13477586588878224	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3568086149136293E-4	0.0	0.0	0.023497660304395405	0.0
2	3.3568086149136293E-4	0.0	0.0	0.12034158884465361	0.0
3	3.3568086149136293E-4	0.0	0.0	0.17237212237581487	0.0
4	3.3568086149136293E-4	0.0	0.0	0.22675242193741565	0.0
5	3.3568086149136293E-4	0.0	0.0	0.4135588213573591	0.0
6	3.3568086149136293E-4	0.0	0.0	0.5634403260132527	0.0
7	3.3568086149136293E-4	0.0	0.0	0.6943558619948842	0.0
8	3.3568086149136293E-4	0.0	0.0	0.9852233284771502	0.0
9	3.3568086149136293E-4	0.0	0.0	1.1032151512913644	0.0
10	3.3568086149136293E-4	0.0	0.0	1.4504770025041793	0.0
11	3.3568086149136293E-4	0.0	0.0	1.8771273774597015	0.0
12	3.3568086149136293E-4	0.0	0.0	2.254096984914502	0.0
13	3.3568086149136293E-4	0.0	0.0	2.4367073735658034	0.0
14	3.3568086149136293E-4	0.0	0.0	2.52079542936939	0.0
15	3.3568086149136293E-4	0.0	0.0	2.6468435928593967	0.0
16	3.3568086149136293E-4	0.0	0.0	2.8949117495015138	0.0
17	3.3568086149136293E-4	0.0	0.0	3.1827580882303574	0.0
18	3.3568086149136293E-4	0.0	0.0	3.5259917691052762	0.0
19	3.3568086149136293E-4	0.0	0.0	3.719511785755047	0.0
20	3.3568086149136293E-4	0.0	0.0	3.9115212385281066	0.0
21	3.3568086149136293E-4	0.0	0.0	4.154386341817108	0.0
22	3.3568086149136293E-4	0.0	0.0	4.42712704177884	0.0
23	5.035212922370444E-4	0.0	0.0	4.682580177373767	0.0
24	5.035212922370444E-4	0.0	0.0	4.9002692160509165	0.0
25	5.035212922370444E-4	0.0	0.0	5.098992286053803	0.0
26	5.035212922370444E-4	0.0	0.0	5.282106195997342	0.0
27	5.035212922370444E-4	0.0	0.0	5.46135977603373	0.0
28	5.035212922370444E-4	0.0	0.0	5.65874012259065	0.0
29	5.035212922370444E-4	0.0	0.0	5.877604044283019	0.0
30	5.035212922370444E-4	0.0	0.0	6.120804828433512	0.0
31	5.035212922370444E-4	0.0	0.0	6.355781431477466	0.0
32	5.035212922370444E-4	0.0	0.0	6.569945821108956	0.0
33	5.035212922370444E-4	0.0	0.0	6.793509274862203	0.0
34	5.035212922370444E-4	0.0	0.0	7.031674846090325	0.0
35	5.035212922370444E-4	0.0	0.0	7.2755469919638	0.0
36	5.035212922370444E-4	0.0	0.0	7.506663265100603	0.0
37	5.035212922370444E-4	0.0	0.0	7.75590630475794	0.0
38	5.035212922370444E-4	0.0	0.0	8.010688078629885	0.0
39	6.713617229827259E-4	0.0	0.0	8.286617746775786	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATA	20	7.030547E-4	45.000004	9
TACGAAT	50	2.1827873E-11	45.0	12
CGTTTTA	725	0.0	41.27586	1
CGTTATT	330	0.0	40.90909	1
CATAGTT	105	0.0	40.714287	36
GTTAGCG	50	1.0804797E-9	40.5	1
TCTACGG	50	1.0804797E-9	40.5	2
CGTTTTT	1225	0.0	40.224487	1
CGTTAGG	45	1.9255822E-8	40.000004	3
TCACGAC	40	3.4544792E-7	39.375004	25
ATAGGCG	70	0.0	38.57143	5
CGTTAAG	35	6.2432227E-6	38.57143	2
GTTAACG	35	6.2432227E-6	38.57143	1
GCTAACG	35	6.2432227E-6	38.57143	1
TGCACGG	35	6.2432227E-6	38.57143	2
CGGTTTA	65	9.094947E-12	38.076927	1
GCTACGA	65	9.094947E-12	38.076927	10
AGGCGAT	350	0.0	37.928574	7
TAGGCAT	85	0.0	37.058823	6
ATAGGCC	55	2.744855E-9	36.81818	5
>>END_MODULE