##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936151.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	465897
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.828998684258536	31.0	31.0	34.0	30.0	34.0
2	31.9994290583541	33.0	31.0	34.0	30.0	34.0
3	31.623453252542944	33.0	31.0	34.0	30.0	34.0
4	35.537837762423884	37.0	35.0	37.0	33.0	37.0
5	31.922190956370184	37.0	35.0	37.0	0.0	37.0
6	33.834482729015214	37.0	35.0	37.0	19.0	37.0
7	20.88786577290689	32.0	0.0	37.0	0.0	37.0
8	28.12515856509057	35.0	17.0	37.0	17.0	37.0
9	34.75483851580929	37.0	32.0	39.0	30.0	39.0
10	36.19225708686684	37.0	35.0	39.0	32.0	39.0
11	36.598846955442944	38.0	35.0	39.0	32.0	39.0
12	36.97580581115569	39.0	37.0	39.0	33.0	39.0
13	36.786321869426075	39.0	37.0	39.0	33.0	39.0
14	38.13516506867398	40.0	37.0	41.0	33.0	41.0
15	38.30692191621753	40.0	38.0	41.0	34.0	41.0
16	38.374677235526306	40.0	38.0	41.0	34.0	41.0
17	38.340354198460176	40.0	38.0	41.0	34.0	41.0
18	38.168114411554484	40.0	37.0	41.0	33.0	41.0
19	38.06364711513489	40.0	37.0	41.0	34.0	41.0
20	37.97514042803452	40.0	36.0	41.0	34.0	41.0
21	37.89266297057075	40.0	36.0	41.0	33.0	41.0
22	37.86395490848836	39.0	36.0	41.0	33.0	41.0
23	37.79745523152113	39.0	36.0	41.0	33.0	41.0
24	37.6573448637789	39.0	35.0	41.0	33.0	41.0
25	37.50862529700771	39.0	35.0	41.0	33.0	41.0
26	37.385143068103034	39.0	35.0	41.0	33.0	41.0
27	37.34211424413551	39.0	35.0	41.0	33.0	41.0
28	37.19966215708622	39.0	35.0	41.0	33.0	41.0
29	37.13963386757159	39.0	35.0	41.0	32.0	41.0
30	36.965185437983074	39.0	35.0	41.0	32.0	41.0
31	36.79897917350831	39.0	35.0	41.0	31.0	41.0
32	36.638666915648734	39.0	35.0	41.0	31.0	41.0
33	36.39888644915078	39.0	35.0	41.0	30.0	41.0
34	36.08786276795085	39.0	35.0	41.0	29.0	41.0
35	35.83224189037491	39.0	35.0	40.0	27.0	41.0
36	35.610325887481565	39.0	35.0	40.0	25.0	41.0
37	35.438283569115065	39.0	35.0	40.0	25.0	41.0
38	35.29182201216149	39.0	35.0	40.0	24.0	41.0
39	35.18068156695578	39.0	35.0	40.0	24.0	41.0
40	35.06761794989021	38.0	34.0	40.0	23.0	41.0
41	34.948222461187775	38.0	34.0	40.0	23.0	41.0
42	34.84724306016137	38.0	34.0	40.0	23.0	41.0
43	34.780211076697206	38.0	34.0	40.0	22.0	41.0
44	34.63978733496889	38.0	34.0	40.0	22.0	41.0
45	34.57235612163203	38.0	34.0	40.0	22.0	41.0
46	34.437519451724306	38.0	34.0	40.0	21.0	41.0
47	34.36662824615741	38.0	33.0	40.0	21.0	41.0
48	34.243472269621826	38.0	33.0	40.0	20.0	41.0
49	34.10378688851828	38.0	33.0	40.0	20.0	41.0
50	33.96166963942674	37.0	33.0	40.0	20.0	41.0
51	32.11399086064087	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	25.0
10	27.0
11	19.0
12	18.0
13	13.0
14	18.0
15	28.0
16	43.0
17	113.0
18	230.0
19	434.0
20	807.0
21	1300.0
22	2175.0
23	3356.0
24	5050.0
25	7124.0
26	8284.0
27	8681.0
28	8743.0
29	8986.0
30	10421.0
31	12761.0
32	16912.0
33	23678.0
34	31872.0
35	40726.0
36	51906.0
37	83899.0
38	84264.0
39	53964.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.433394076373105	18.877992345947707	24.668542617788912	17.020070959890276
2	35.038860520673026	24.112411112327404	25.067987130202596	15.780741236796974
3	29.487418893017125	23.907000903633207	31.182858013681138	15.42272218966853
4	25.870739669927044	23.914298653994337	32.72311261931285	17.491849056765766
5	21.46203989293771	35.518365647342655	26.74453795581427	16.27505650390537
6	23.823506053913203	33.18415873036315	29.830627799706804	13.161707416016846
7	45.77041706643314	42.46797038830471	9.177779637988655	2.583832907273496
8	79.25442748075218	4.9800707023226165	12.591195049549578	3.174306767375622
9	73.74462595809803	6.845933757890693	14.042374172832192	5.36706611117908
10	39.79892551357918	26.006391970757488	21.37747184463519	12.817210671028146
11	28.094192493190555	24.84175686900753	30.24617029085828	16.817880346943635
12	25.16521892177885	22.682266681262167	34.14939353548102	18.003120861477967
13	22.063675018298035	23.990281113636705	36.13502555285825	17.811018315207008
14	19.510106311051583	26.27512089582032	34.69457841540083	19.520194377727265
15	18.966638548863806	25.356248269467287	37.57654588889819	18.10056729277072
16	22.952927363773536	24.628190351086186	34.191033640482765	18.22784864465751
17	23.702019974372018	23.243120260486762	32.424334133939475	20.630525631201746
18	24.057034065469406	24.203632991841545	32.991626904659185	18.747706038029865
19	23.321034477577662	25.964107946606223	31.32366166770767	19.391195908108443
20	24.532246397808958	25.480095385890017	32.04399255629463	17.943665660006396
21	23.617237286352992	25.182819378532166	33.336338289364384	17.863605045750454
22	22.903989508410657	22.80911875371595	33.66602489391432	20.620866843959075
23	21.04070212944063	24.83188344204835	33.97102793106631	20.156386497444714
24	21.967516425304307	24.688933390856775	33.62803366409314	19.71551651974578
25	20.890025048454916	25.858075926653317	32.7591720916855	20.492726933206267
26	20.415885914697885	25.94114149694031	32.40458728002112	21.238385308340685
27	20.965578228664274	25.424932978748522	32.721610141297326	20.887878651289878
28	19.290315241351628	25.961961549441188	34.69500769483383	20.052715514373347
29	20.661648390094804	25.360111784364353	33.66301993788326	20.315219887657573
30	20.751153151876917	25.20664438706409	33.790945208919574	20.25125725213942
31	21.867279677697002	25.476017231276444	32.452881216234495	20.20382187479207
32	22.57237114641219	26.21630961349826	32.27859376643335	18.932725473656195
33	21.743861840707282	25.709330603116136	31.207326941362574	21.339480614814004
34	21.10874291957235	26.671560452202954	32.54410309574863	19.675593532476064
35	21.083200793308393	27.341021727978504	31.11267082638437	20.463106652328733
36	22.91021406018927	26.87310714600008	30.671800848685436	19.54487794512521
37	21.471698680180385	28.203014829458013	30.430760447051604	19.89452604331
38	21.77627243789936	27.517884854377684	29.779114267745875	20.926728439977076
39	22.08664146796395	27.32470910952421	29.769455480503204	20.81919394200864
40	22.98018660776953	26.405836483171175	30.286522557561007	20.327454351498293
41	21.26607383176968	27.058126581626414	30.41595030661283	21.259849279991073
42	22.07333380554071	25.767068686855676	31.50481758843693	20.654779919166682
43	22.700940336598002	25.354960431168262	30.036467287834007	21.90763194439973
44	21.042204607456156	26.330927222111328	30.52842151806086	22.098446652371663
45	20.556904208440923	26.653745355733133	29.858316323135803	22.931034112690142
46	21.764467253491652	26.973773173040392	30.096995687888096	21.16476388557986
47	20.769397527779745	26.715561594086246	31.86777334904496	20.647267529089046
48	21.48543562203663	26.14891274251605	30.79307228851012	21.5725793469372
49	20.92866019742561	26.02485098637682	31.616215601302432	21.430273214895138
50	20.49680508781984	26.124014535401603	31.5977565856831	21.781423791095456
51	20.405368568589193	26.11564358645795	30.59259879329551	22.88638905165734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	325.0
1	691.0
2	1057.0
3	6524.5
4	11992.0
5	8330.0
6	4668.0
7	4115.0
8	3562.0
9	3401.0
10	3240.0
11	3120.0
12	3000.0
13	2984.0
14	2968.0
15	2834.5
16	2701.0
17	2537.5
18	2374.0
19	2350.0
20	2326.0
21	2526.0
22	2726.0
23	3157.5
24	3589.0
25	3921.0
26	5156.5
27	6060.0
28	7297.0
29	8534.0
30	9528.0
31	10522.0
32	11946.5
33	13371.0
34	14887.0
35	16403.0
36	17657.5
37	18912.0
38	20327.5
39	21743.0
40	23149.0
41	24555.0
42	26062.0
43	27569.0
44	29491.5
45	31414.0
46	34107.0
47	36800.0
48	34547.5
49	32295.0
50	30725.5
51	29156.0
52	26940.0
53	24724.0
54	23357.5
55	21991.0
56	21064.5
57	20138.0
58	19150.5
59	18163.0
60	16670.0
61	15177.0
62	13748.5
63	12320.0
64	10592.5
65	8865.0
66	7608.0
67	6351.0
68	5423.0
69	4495.0
70	3704.5
71	2914.0
72	2372.5
73	1831.0
74	1554.5
75	969.0
76	660.0
77	514.0
78	368.0
79	300.0
80	232.0
81	180.5
82	129.0
83	98.0
84	67.0
85	47.5
86	28.0
87	27.0
88	26.0
89	18.5
90	11.0
91	11.0
92	11.0
93	6.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	465897.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.223361281587565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74439731868723	30.399919331611812
2	7.995068716488356	6.591672123418362
3	3.860211455688335	4.773926743834898
4	2.5564659726799417	4.2154448158348155
5	1.9085213877826044	3.933783334109959
6	1.5775646839384574	3.901951134664112
7	1.2462825042005505	3.5963167750706675
8	1.0827148310330734	3.570651571568753
9	0.8998337866223881	3.338475595138231
>10	5.089910639518107	31.64879043622048
>50	0.022454870426841696	0.5739124464048857
>100	0.011762074985487009	0.8787207848755896
>500	0.0032078386324055483	0.9159677908058566
>1k	0.0016039193162027741	1.6604671164416083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4122	0.884744911428921	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2401	0.5153499593257737	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC	1011	0.21700075338540492	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	931	0.19982957606509594	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCG	877	0.18823903137388737	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGC	655	0.1405890143100299	No Hit
GAATCTATCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC	615	0.13200342564987538	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCG	577	0.12384711642272864	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	501	0.10753449796843507	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02640068512997508	0.0
2	0.0	0.0	0.0	0.16656042000699725	0.0
3	0.0	0.0	0.0	0.2255863420455594	0.0
4	0.0	0.0	0.0	0.34836025988576874	0.0
5	0.0	0.0	0.0	0.8012500617089185	0.0
6	0.0	0.0	0.0	1.0347780732651208	0.0
7	0.0	0.0	0.0	1.2461981940214253	0.0
8	0.0	0.0	0.0	1.6514379787807176	0.0
9	0.0	0.0	0.0	1.828730384612908	0.0
10	0.0	0.0	0.0	2.5357536107766308	0.0
11	0.0	0.0	0.0	2.9210319019010638	0.0
12	0.0	0.0	0.0	3.512364320869205	0.0
13	0.0	0.0	0.0	3.701461911109108	0.0
14	0.0	0.0	0.0	3.8004108204173885	0.0
15	0.0	0.0	0.0	3.9358484815313255	0.0
16	0.0	0.0	0.0	4.150058918602181	0.0
17	0.0	0.0	0.0	4.423724557144605	0.0
18	0.0	0.0	0.0	4.7282983148635855	0.0
19	0.0	0.0	0.0	4.919542302268527	0.0
20	0.0	0.0	0.0	5.114435164854034	0.0
21	0.0	0.0	0.0	5.345816779245198	0.0
22	0.0	0.0	0.0	5.604242997915849	0.0
23	0.0	0.0	0.0	5.870396246380638	0.0
24	0.0	0.0	0.0	6.083748124585477	0.0
25	0.0	0.0	0.0	6.289372972996177	0.0
26	0.0	0.0	0.0	6.487270791612739	0.0
27	0.0	0.0	0.0	6.70019339038457	0.0
28	2.1463971650386243E-4	0.0	0.0	6.908179275676813	0.0
29	2.1463971650386243E-4	0.0	0.0	7.147717199295124	0.0
30	2.1463971650386243E-4	0.0	0.0	7.402923822218216	0.0
31	2.1463971650386243E-4	0.0	0.0	7.644822782718069	0.0
32	2.1463971650386243E-4	0.0	0.0	7.898526927625634	0.0
33	6.439191495115873E-4	0.0	0.0	8.123683990238186	0.0
34	6.439191495115873E-4	0.0	0.0	8.372451421666161	0.0
35	6.439191495115873E-4	0.0	0.0	8.623365250259177	0.0
36	6.439191495115873E-4	0.0	0.0	8.85689326181538	0.0
37	6.439191495115873E-4	0.0	0.0	9.115963399635541	0.0
38	6.439191495115873E-4	0.0	0.0	9.396926788539098	0.0
39	6.439191495115873E-4	0.0	0.0	9.777483005900446	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTT	20	7.028964E-4	45.0	27
TGCGATG	25	3.8872222E-5	45.0	1
TGTTACG	25	3.8872222E-5	45.0	1
ACGTATA	20	7.028964E-4	45.0	38
TAGTGCG	20	7.028964E-4	45.0	1
TCGACGG	20	7.028964E-4	45.0	2
TCAACGG	25	3.8872222E-5	45.0	2
TATAGCG	30	2.162711E-6	44.999996	1
CGTTATT	320	0.0	44.296875	1
AGGCGAT	285	0.0	43.421055	7
CGTTTTA	940	0.0	43.085106	1
CGTTTTT	1855	0.0	42.331535	1
AGGTACC	70	0.0	41.785713	7
CGGTTTA	70	0.0	41.785713	1
GTTACGG	45	1.924127E-8	40.0	2
CTTACGG	45	1.924127E-8	40.0	2
GCGATAT	40	3.4523146E-7	39.375	9
TAAGGAT	175	0.0	38.57143	5
ACAACGG	35	6.240294E-6	38.571426	2
CGGGTAT	35	6.240294E-6	38.571426	6
>>END_MODULE