##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936150.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	333734
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.828384282092923	31.0	31.0	34.0	30.0	34.0
2	32.017436041877666	33.0	31.0	34.0	30.0	34.0
3	31.74441321531519	33.0	31.0	34.0	30.0	34.0
4	35.60862243583213	37.0	35.0	37.0	33.0	37.0
5	31.96986821840148	37.0	35.0	37.0	0.0	37.0
6	33.875277915945034	37.0	35.0	37.0	19.0	37.0
7	20.849401019973993	32.0	0.0	37.0	0.0	37.0
8	28.07785841418615	35.0	17.0	37.0	17.0	37.0
9	34.681392965655284	35.0	32.0	39.0	30.0	39.0
10	36.16842155728814	37.0	35.0	39.0	32.0	39.0
11	36.616143395638446	38.0	35.0	39.0	32.0	39.0
12	36.96389639653137	39.0	37.0	39.0	33.0	39.0
13	36.71511143605386	39.0	35.0	39.0	33.0	39.0
14	38.094308641013505	40.0	37.0	41.0	33.0	41.0
15	38.264045617168165	40.0	37.0	41.0	33.0	41.0
16	38.33522805587683	40.0	37.0	41.0	34.0	41.0
17	38.283639065842856	40.0	37.0	41.0	34.0	41.0
18	38.145199470236776	40.0	37.0	41.0	33.0	41.0
19	38.062819490971854	40.0	37.0	41.0	34.0	41.0
20	38.018745467947525	40.0	36.0	41.0	34.0	41.0
21	37.934178117902285	40.0	36.0	41.0	33.0	41.0
22	37.92074826059077	40.0	36.0	41.0	33.0	41.0
23	37.86280690609887	40.0	36.0	41.0	33.0	41.0
24	37.73454607561711	40.0	36.0	41.0	33.0	41.0
25	37.59728406455441	39.0	35.0	41.0	33.0	41.0
26	37.41780280103316	39.0	35.0	41.0	33.0	41.0
27	37.378403159402396	39.0	35.0	41.0	33.0	41.0
28	37.288259512066496	39.0	35.0	41.0	33.0	41.0
29	37.27917143593401	39.0	35.0	41.0	33.0	41.0
30	37.118264845655524	39.0	35.0	41.0	32.0	41.0
31	36.99273373405167	39.0	35.0	41.0	32.0	41.0
32	36.866357638118984	39.0	35.0	41.0	31.0	41.0
33	36.652004290842406	39.0	35.0	41.0	31.0	41.0
34	36.44335608598465	39.0	35.0	41.0	30.0	41.0
35	36.260980301677385	39.0	35.0	41.0	30.0	41.0
36	36.10264162476703	39.0	35.0	40.0	29.0	41.0
37	36.02951152714437	39.0	35.0	40.0	29.0	41.0
38	35.920313782833034	39.0	35.0	40.0	28.0	41.0
39	35.89290872371409	39.0	35.0	40.0	28.0	41.0
40	35.77638178908951	39.0	35.0	40.0	27.0	41.0
41	35.679631083437705	39.0	35.0	40.0	27.0	41.0
42	35.58161589769097	38.0	35.0	40.0	27.0	41.0
43	35.48951859864443	38.0	35.0	40.0	26.0	41.0
44	35.37015407480209	38.0	35.0	40.0	26.0	41.0
45	35.26341637351903	38.0	34.0	40.0	25.0	41.0
46	35.12388309252279	38.0	34.0	40.0	24.0	41.0
47	35.08183763116734	38.0	34.0	40.0	24.0	41.0
48	34.9551798737917	38.0	34.0	40.0	24.0	41.0
49	34.83667531626984	38.0	34.0	40.0	24.0	41.0
50	34.663022047498906	38.0	34.0	40.0	23.0	41.0
51	32.752425584447494	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	14.0
10	21.0
11	9.0
12	11.0
13	8.0
14	9.0
15	17.0
16	38.0
17	68.0
18	117.0
19	245.0
20	402.0
21	697.0
22	1131.0
23	1867.0
24	2801.0
25	3851.0
26	4517.0
27	4934.0
28	5373.0
29	5924.0
30	7000.0
31	8973.0
32	12026.0
33	17712.0
34	24568.0
35	31023.0
36	37828.0
37	60595.0
38	62286.0
39	39658.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.80375388782683	20.08605656001486	24.561776744353285	14.54841280780502
2	33.580636075437326	25.53380836234846	24.19711506768864	16.68844049452558
3	29.840831320752454	24.521025727076054	30.038293970647285	15.59984898152421
4	28.124194717949024	23.82196599687176	30.773310480802074	17.280528804377138
5	22.923645777775114	35.98824213295619	24.131493944278976	16.956618144989722
6	25.130792787069943	34.87807655198451	26.884884368988477	13.106246291957069
7	46.17090257510472	42.88804856562412	7.869740571832658	3.071308287438499
8	77.97617264048614	6.910893106486003	11.450736215069487	3.662198037958374
9	71.75924538704477	8.858851660304314	12.950733218671157	6.431169733979757
10	39.73164256563611	26.205301227924032	20.36292376563371	13.70013244080615
11	31.222470590350397	25.28510730102417	26.74555184668029	16.746870261945144
12	29.612206128233865	22.63359441950775	30.05926875895174	17.694930693306645
13	23.821067077372998	25.966787920919053	31.025007940455573	19.187137061252376
14	18.620218497366167	28.943110381321652	31.037892453271166	21.398778668041015
15	19.00106072500854	25.826556479112107	36.29477368203419	18.87760911384516
16	21.46679691011404	24.815871322670148	34.73964294917509	18.977688818040715
17	21.93813036729851	23.84084330634577	31.072950313722902	23.148076012632814
18	22.569471495262693	25.395374759539035	31.589829025511335	20.445324719686937
19	24.140483139266603	27.061971510244685	28.362708024954003	20.43483732553471
20	26.110914680553975	25.507739696884347	29.91843803747895	18.46290758508273
21	23.84683610300419	26.99125650967537	29.547783564155882	19.614123823164554
22	21.8847944770386	25.99075910755272	29.24275021424248	22.8816962011662
23	21.67594551349278	26.709894706562707	30.491349398023576	21.12281038192093
24	22.413958421976783	24.456003883332233	31.908346167906178	21.221691526784806
25	21.034416631209286	27.708893909520754	29.688314645795757	21.568374813474204
26	20.43993120269436	28.80048182085134	29.61400396723139	21.145583009222914
27	21.076965487484046	28.130487154440363	29.886676215189347	20.90587114288625
28	19.14279036598009	27.966284525999747	32.24903665793716	20.641888450083
29	21.35323341343705	27.05897511191548	31.611702733314555	19.976088741332916
30	21.66485883967471	27.087440896042956	30.065261555610157	21.182438708672176
31	22.69292310642608	27.475474479675434	28.9092510802016	20.922351333696895
32	24.49136138361689	27.670240371073966	28.396567326074056	19.441830919235077
33	22.354030455392618	28.074454505684166	28.33304368149484	21.23847135742837
34	20.784816650386237	28.51432578041194	29.485758118741273	21.21509945046055
35	21.38978947305339	27.09163585370385	29.575949708450445	21.94262496479232
36	21.310085277496448	28.99674591141448	29.43422006747889	20.25894874361018
37	22.226383886568346	29.415342758004876	29.258031845721444	19.100241509705334
38	20.72488868380207	28.473574763134714	28.445108979007234	22.356427574055985
39	22.891584315652587	26.147470740170313	29.050081801674388	21.910863142502713
40	21.53121947419202	27.51502693762098	29.51392426303583	21.43982932515117
41	19.772333654946753	27.92763098755296	29.585837822936828	22.714197534563453
42	21.15067688638257	27.789197384743535	29.555873839644747	21.50425188922915
43	22.495460456531248	27.002343183493444	28.813965613332776	21.688230746642535
44	21.23397675993456	27.52221829361108	28.69321076066568	22.550594185788682
45	20.495963851450558	28.171238171717594	27.667843252410602	23.664954724421243
46	21.376006040739032	29.139074832051875	27.0233179717979	22.461601155411195
47	20.59544427598027	28.149364463914374	30.070355432769812	21.184835827335544
48	20.766238980745143	27.260333079638276	29.53579797083905	22.43762996877753
49	20.823470188833024	26.053084192800252	30.435316749267383	22.68812886909934
50	19.268039816141	27.224376299687776	30.07574894976239	23.43183493440884
51	19.194927696908316	27.589337616185343	28.304877537200284	24.910857149706054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	289.0
1	619.5
2	950.0
3	3801.5
4	6653.0
5	4542.0
6	2431.0
7	2147.5
8	1864.0
9	1800.0
10	1736.0
11	1736.0
12	1736.0
13	1681.5
14	1627.0
15	1601.0
16	1575.0
17	1522.5
18	1470.0
19	1493.5
20	1517.0
21	1822.0
22	2127.0
23	2187.5
24	2248.0
25	2831.5
26	3966.0
27	4517.0
28	5362.0
29	6207.0
30	6727.5
31	7248.0
32	8208.0
33	9168.0
34	10259.5
35	11351.0
36	12086.5
37	12822.0
38	13966.5
39	15111.0
40	16402.0
41	17693.0
42	19365.5
43	21038.0
44	21993.5
45	22949.0
46	24717.5
47	26486.0
48	24543.0
49	22600.0
50	21788.5
51	20977.0
52	19494.0
53	18011.0
54	16836.0
55	15661.0
56	15164.5
57	14668.0
58	14552.5
59	14437.0
60	13903.5
61	13370.0
62	12003.0
63	10636.0
64	8839.5
65	7043.0
66	5736.0
67	4429.0
68	3572.0
69	2715.0
70	2298.0
71	1881.0
72	1569.5
73	1258.0
74	1007.0
75	607.0
76	458.0
77	354.0
78	250.0
79	212.0
80	174.0
81	125.0
82	76.0
83	61.0
84	46.0
85	36.0
86	26.0
87	20.5
88	15.0
89	14.0
90	13.0
91	7.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	333734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.74159416171736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5358871676762	29.621749783013442
2	7.642385422640801	6.07441159788031
3	3.571657305913859	4.258300654090842
4	2.337051848955476	3.715126644643188
5	1.7581065431847773	3.493497836615461
6	1.4460975517444896	3.4482133211809547
7	1.143092703519088	3.1799828418733127
8	1.017973757944102	3.2364719964394246
9	0.9021156309450711	3.2266361962764547
>10	5.573737418510468	34.88805483454519
>50	0.045407146715168226	1.2370206680035025
>100	0.021946787578997977	1.7444006502359142
>500	0.0030271431143445485	0.7714461496301931
>1k	0.0015135715571722743	1.1046868255718125
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2380	0.7131428023515735	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1293	0.38743430396663214	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCG	755	0.22622807385522603	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTC	700	0.2097478830445804	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGC	593	0.1776864209220517	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCG	517	0.15491379362006868	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	478	0.14322784013615636	No Hit
GAATCTATCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTC	476	0.14262856047031466	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGC	392	0.11745881450496504	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	375	0.11236493734531093	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04704345376857018	0.0
2	0.0	0.0	0.0	0.2540945783168631	0.0
3	0.0	0.0	0.0	0.34878076551984516	0.0
4	0.0	0.0	0.0	0.47253201651614757	0.0
5	0.0	0.0	0.0	0.9447643931993743	0.0
6	0.0	0.0	0.0	1.1793823823763836	0.0
7	0.0	0.0	0.0	1.458946346491517	0.0
8	0.0	0.0	0.0	2.043244020687134	0.0
9	0.0	0.0	0.0	2.278161649697064	0.0
10	0.0	0.0	0.0	3.2409044328716883	0.0
11	0.0	0.0	0.0	3.9387656037442995	0.0
12	0.0	0.0	0.0	4.797833004728316	0.0
13	0.0	0.0	0.0	5.052526862711021	0.0
14	0.0	0.0	0.0	5.18586658836079	0.0
15	0.0	0.0	0.0	5.40250618756255	0.0
16	0.0	0.0	0.0	5.79263725002547	0.0
17	0.0	0.0	0.0	6.2444941180700795	0.0
18	0.0	0.0	0.0	6.7490875967087565	0.0
19	0.0	0.0	0.0	7.053222027123398	0.0
20	0.0	0.0	0.0	7.351064021046702	0.0
21	0.0	0.0	0.0	7.7169242570430345	0.0
22	0.0	0.0	0.0	8.117542713658183	0.0
23	0.0	0.0	0.0	8.560410386715168	0.0
24	0.0	0.0	0.0	8.895108080087734	0.0
25	0.0	0.0	0.0	9.183661239190492	0.0
26	0.0	0.0	0.0	9.473712597457856	0.0
27	0.0	0.0	0.0	9.749381243745018	0.0
28	0.0	0.0	0.0	10.048721436832928	0.0
29	0.0	0.0	0.0	10.370534617389897	0.0
30	0.0	0.0	0.0	10.72530817956816	0.0
31	0.0	0.0	0.0	11.070493267092955	0.0
32	0.0	0.0	0.0	11.40519096046552	0.0
33	0.0	0.0	0.0	11.709625030713083	0.0
34	0.0	0.0	0.0	12.031438211270054	0.0
35	0.0	0.0	0.0	12.370630502136432	0.0
36	0.0	0.0	0.0	12.731097221140189	0.0
37	0.0	0.0	0.0	13.06699347384444	0.0
38	0.0	0.0	0.0	13.433752629339534	0.0
39	0.0	0.0	0.0	13.772944920205912	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	20	7.026085E-4	45.0	40
GGTACGT	20	7.026085E-4	45.0	8
ATAGCGG	40	6.7902874E-9	45.0	2
CCCGTGA	20	7.026085E-4	45.0	21
CTCGGTT	20	7.026085E-4	45.0	36
TCTCGAT	20	7.026085E-4	45.0	23
TTGCGTG	20	7.026085E-4	45.0	1
CTCGATA	20	7.026085E-4	45.0	24
AATAGCG	25	3.884836E-5	45.0	1
ATACGAG	20	7.026085E-4	45.0	1
ATTGCGA	20	7.026085E-4	45.0	1
TCAAGCG	25	3.884836E-5	45.0	17
CGTTTAG	25	3.884836E-5	45.0	2
CGTCAGC	20	7.026085E-4	45.0	29
CTTACGG	20	7.026085E-4	45.0	2
AACACGT	25	3.884836E-5	45.0	41
CCCGACC	20	7.026085E-4	45.0	10
GGTACCT	30	2.1608557E-6	44.999996	8
CTACGGG	30	2.1608557E-6	44.999996	3
CGTTTTA	440	0.0	42.954544	1
>>END_MODULE