##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936146.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346010
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93934279356088	31.0	31.0	34.0	30.0	34.0
2	32.12583740354325	33.0	31.0	34.0	30.0	34.0
3	31.775110545937977	33.0	31.0	34.0	30.0	34.0
4	35.65864570388139	37.0	35.0	37.0	35.0	37.0
5	31.99702320742175	37.0	35.0	37.0	0.0	37.0
6	33.911097945146096	37.0	35.0	37.0	19.0	37.0
7	20.838140516170053	32.0	0.0	37.0	0.0	37.0
8	28.141027715961968	35.0	17.0	37.0	17.0	37.0
9	34.82873038351493	37.0	32.0	39.0	32.0	39.0
10	36.26658478078668	37.0	35.0	39.0	32.0	39.0
11	36.70225138001792	38.0	35.0	39.0	33.0	39.0
12	37.096245773243545	39.0	37.0	39.0	34.0	39.0
13	36.95447530418196	39.0	37.0	39.0	33.0	39.0
14	38.41691858616803	40.0	38.0	41.0	34.0	41.0
15	38.57989942487211	40.0	38.0	41.0	34.0	41.0
16	38.635418051501404	40.0	38.0	41.0	35.0	41.0
17	38.57767116557325	40.0	38.0	41.0	34.0	41.0
18	38.44482818415653	40.0	38.0	41.0	34.0	41.0
19	38.37612785757637	40.0	37.0	41.0	34.0	41.0
20	38.28678361897055	40.0	37.0	41.0	34.0	41.0
21	38.212696164850726	40.0	37.0	41.0	34.0	41.0
22	38.14186006184792	40.0	37.0	41.0	34.0	41.0
23	38.08289355798966	40.0	37.0	41.0	34.0	41.0
24	37.93905089448282	40.0	36.0	41.0	33.0	41.0
25	37.79575156787376	40.0	36.0	41.0	33.0	41.0
26	37.66479870523973	40.0	36.0	41.0	33.0	41.0
27	37.62449351174821	40.0	36.0	41.0	33.0	41.0
28	37.51106904424728	40.0	36.0	41.0	33.0	41.0
29	37.47358746857027	40.0	36.0	41.0	33.0	41.0
30	37.340391896187974	40.0	36.0	41.0	33.0	41.0
31	37.19186150689286	40.0	35.0	41.0	32.0	41.0
32	37.02371029739025	40.0	35.0	41.0	31.0	41.0
33	36.79867344874425	39.0	35.0	41.0	31.0	41.0
34	36.59819658391376	39.0	35.0	41.0	30.0	41.0
35	36.387578393688045	39.0	35.0	41.0	30.0	41.0
36	36.19700586688246	39.0	35.0	41.0	29.0	41.0
37	36.09164475015173	39.0	35.0	41.0	28.0	41.0
38	35.96766856449236	39.0	35.0	41.0	27.0	41.0
39	35.90155775844629	39.0	35.0	41.0	27.0	41.0
40	35.816973497875786	39.0	35.0	41.0	26.0	41.0
41	35.7064940319644	39.0	35.0	40.0	26.0	41.0
42	35.59916765411404	39.0	35.0	40.0	25.0	41.0
43	35.5159706366868	39.0	35.0	40.0	25.0	41.0
44	35.391610069073145	39.0	35.0	40.0	24.0	41.0
45	35.35823820120806	39.0	35.0	40.0	24.0	41.0
46	35.2296552122771	39.0	35.0	40.0	24.0	41.0
47	35.22467847750065	38.0	34.0	40.0	24.0	41.0
48	35.09237305280194	38.0	34.0	40.0	23.0	41.0
49	34.948250050576576	38.0	34.0	40.0	23.0	41.0
50	34.81025692899049	38.0	34.0	40.0	23.0	41.0
51	33.0468165659952	36.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	17.0
10	24.0
11	20.0
12	9.0
13	11.0
14	10.0
15	14.0
16	26.0
17	51.0
18	117.0
19	261.0
20	437.0
21	807.0
22	1337.0
23	2088.0
24	3276.0
25	4489.0
26	5227.0
27	5270.0
28	5336.0
29	5509.0
30	6403.0
31	8068.0
32	10718.0
33	15663.0
34	21916.0
35	28655.0
36	37668.0
37	65256.0
38	70388.0
39	46924.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.86130458657264	19.85144938007572	24.68454668940204	16.6026993439496
2	35.064593508858124	24.30363284298142	24.800439293662034	15.831334354498425
3	31.260368197450937	23.994104216641137	29.720239299442213	15.02528828646571
4	27.47348342533453	24.904193520418485	29.974567209040202	17.647755845206785
5	22.50946504436288	36.268315944625876	25.605618334730213	15.616600676281031
6	24.516054449293375	35.598393110025725	27.123782549637294	12.761769891043611
7	46.9642495881622	42.97621456027282	7.662784312592122	2.396751538972862
8	81.77335915146962	4.997254414612295	10.733215803011474	2.496170630906621
9	75.45157654403052	8.14571833184012	12.443860004046126	3.9588451200832346
10	38.1205745498685	30.91760353747002	19.35059680356059	11.611225109100893
11	28.08993959712147	27.124649576601833	29.108118262477962	15.677292563798733
12	27.152683448455246	23.99872836045201	31.399092511777116	17.44949567931563
13	22.51033207132742	25.74781075691454	32.17363660009826	19.56822057165978
14	19.024883673882258	29.223143839773417	31.59793069564463	20.15404179069969
15	19.286725817172915	26.831594462587788	36.05155920349123	17.83012051674807
16	22.53923297014537	25.552440680905175	34.44351319326031	17.46481315568914
17	21.45891737233028	25.377301234068376	32.240975694344094	20.922805699257246
18	22.48403225340308	26.76801248518829	31.324528192826794	19.423427068581834
19	24.081673940059538	26.954134273575907	29.888153521574523	19.076038264790036
20	25.662553105401575	26.059073437183894	31.084650732637787	17.19372272477674
21	24.40045085402156	25.994046414843503	30.916736510505476	18.68876622062946
22	23.1643594115777	25.090893326782464	30.865292910609522	20.879454351030315
23	22.73749313603653	25.99578046877258	31.695037715672957	19.571688679517933
24	21.734053929077195	25.408225195803592	32.95309384121846	19.904627033900756
25	21.863240946793443	27.1194474148146	31.32857431866131	19.68873731973064
26	21.085228750614142	29.22169879483252	30.072252247044883	19.620820207508455
27	21.553134302476806	28.34600156064854	31.464697552093867	18.636166584780785
28	20.60634085720066	28.276639403485447	32.839224299875724	18.277795439438165
29	21.121643883124765	26.87176671194474	32.37594289182393	19.63064651310656
30	21.151700817895435	26.962804543221292	32.04820669922835	19.837287939654924
31	22.495592612930263	27.787058177509323	30.186699806363976	19.53064940319644
32	23.360885523539782	28.0725990578307	30.256928990491605	18.309586428137912
33	22.291552267275513	28.726337389092798	29.94335423831681	19.038756105314874
34	21.444177913933125	28.867951793300772	30.194503049044823	19.49336724372128
35	22.828530967313082	27.20065894049305	29.487587063957687	20.48322302823618
36	21.73058582121904	29.785844339758967	29.354353920406922	19.12921591861507
37	21.987803820698822	30.343920695933647	29.30030923961735	18.367966243750182
38	22.220745065171528	28.482702812057454	28.78905233952776	20.507499783243258
39	23.067830409525737	28.18704661714979	29.17892546458195	19.566197508742523
40	22.828530967313082	27.90526285367475	30.091904858241094	19.174301320771075
41	21.048235600127164	28.15005346666281	30.416172942978527	20.385537990231494
42	21.919308690500277	28.709863876766565	29.175168347735614	20.195659084997544
43	22.47073783994682	28.41189560995347	28.94511719314471	20.172249356955003
44	21.40342764659981	28.970260975116325	28.6910782925349	20.935233085748965
45	21.13031415277015	28.450044796393172	28.73240657784457	21.68723447299211
46	20.448830958642812	30.16126701540418	28.279818502355425	21.110083523597584
47	22.276523799890178	28.192537787925204	29.543943816652696	19.98699459553192
48	21.986358775757925	27.04025895205341	30.312707725210252	20.66067454697841
49	20.561255455044652	28.215080489003206	30.126585936822636	21.097078119129506
50	19.862142712638363	28.42461200543337	30.057223779659548	21.65602150226872
51	20.37628970260975	28.385017774052773	29.508395711106616	21.730296812230858
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	194.0
1	473.5
2	753.0
3	4231.5
4	7710.0
5	5365.0
6	3020.0
7	2632.5
8	2245.0
9	2107.5
10	1970.0
11	1934.5
12	1899.0
13	1856.5
14	1814.0
15	1766.0
16	1718.0
17	1709.5
18	1701.0
19	1843.5
20	1986.0
21	2426.5
22	2867.0
23	2718.5
24	2570.0
25	3095.5
26	4434.5
27	5248.0
28	6405.5
29	7563.0
30	8358.0
31	9153.0
32	9892.5
33	10632.0
34	11561.5
35	12491.0
36	13640.5
37	14790.0
38	16096.0
39	17402.0
40	18703.0
41	20004.0
42	21114.5
43	22225.0
44	22792.0
45	23359.0
46	23819.0
47	24279.0
48	23769.0
49	23259.0
50	22002.0
51	20745.0
52	19222.0
53	17699.0
54	16541.5
55	15384.0
56	14954.0
57	14524.0
58	14435.5
59	14347.0
60	13576.0
61	12805.0
62	11057.0
63	9309.0
64	7729.5
65	6150.0
66	5009.0
67	3868.0
68	3167.0
69	2466.0
70	2046.5
71	1627.0
72	1358.0
73	1089.0
74	897.5
75	554.0
76	402.0
77	301.0
78	200.0
79	157.0
80	114.0
81	80.5
82	47.0
83	31.0
84	15.0
85	14.0
86	13.0
87	14.0
88	15.0
89	10.5
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	346010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.52461184084664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.25016823085495	27.370860192378714
2	8.645407599953433	6.8341275918813
3	4.843328234697024	5.742920054826385
4	3.3188416284812727	5.247037085078626
5	2.5265282790236365	4.993002476666576
6	1.945060012991087	4.612664521237491
7	1.6567315853023747	4.583717098348281
8	1.3242077919424327	4.18710391785194
9	1.200804592540906	4.2715201875196795
>10	5.248107049739847	29.358003747953553
>50	0.030425723152641023	0.8142244282186604
>100	0.007420908086010006	0.4335099801538833
>500	0.0014841816172020013	0.3425843415559781
>1k	0.0014841816172020013	1.2087243763289264
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2677	0.7736770613566082	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1444	0.4173289789312448	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	586	0.16935926707320598	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	582	0.16820323112048785	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.890089881795324E-4	0.0	0.0	0.023987746018901187	0.0
2	2.890089881795324E-4	0.0	0.0	0.08901476835929598	0.0
3	2.890089881795324E-4	0.0	0.0	0.13236611658622582	0.0
4	2.890089881795324E-4	0.0	0.0	0.18583277939943932	0.0
5	2.890089881795324E-4	0.0	0.0	0.37166555879887864	0.0
6	2.890089881795324E-4	0.0	0.0	0.5098118551486951	0.0
7	2.890089881795324E-4	0.0	0.0	0.6164561717869426	0.0
8	2.890089881795324E-4	0.0	0.0	0.8684720094794948	0.0
9	2.890089881795324E-4	0.0	0.0	0.9999710991011821	0.0
10	2.890089881795324E-4	0.0	0.0	1.272506574954481	0.0
11	2.890089881795324E-4	0.0	0.0	1.521054304788879	0.0
12	2.890089881795324E-4	0.0	0.0	1.7823184301031763	0.0
13	2.890089881795324E-4	0.0	0.0	1.9343371578856103	0.0
14	2.890089881795324E-4	0.0	0.0	2.0219068813040084	0.0
15	2.890089881795324E-4	0.0	0.0	2.1010953440652003	0.0
16	2.890089881795324E-4	0.0	0.0	2.251669026906737	0.0
17	2.890089881795324E-4	0.0	0.0	2.4340336984480215	0.0
18	2.890089881795324E-4	0.0	0.0	2.6348949452327965	0.0
19	2.890089881795324E-4	0.0	0.0	2.765527007889945	0.0
20	2.890089881795324E-4	0.0	0.0	2.896737088523453	0.0
21	2.890089881795324E-4	0.0	0.0	3.0597381578567093	0.0
22	2.890089881795324E-4	0.0	0.0	3.2449929192797895	0.0
23	2.890089881795324E-4	0.0	0.0	3.4325597526083063	0.0
24	2.890089881795324E-4	0.0	0.0	3.574174156816277	0.0
25	2.890089881795324E-4	0.0	0.0	3.712031444177914	0.0
26	2.890089881795324E-4	0.0	0.0	3.8377503540360105	0.0
27	2.890089881795324E-4	0.0	0.0	3.9779197133030837	0.0
28	5.780179763590648E-4	0.0	0.0	4.13456258489639	0.0
29	5.780179763590648E-4	0.0	0.0	4.292361492442415	0.0
30	5.780179763590648E-4	0.0	0.0	4.467500939279212	0.0
31	5.780179763590648E-4	0.0	0.0	4.637149215340597	0.0
32	5.780179763590648E-4	0.0	0.0	4.816334788011907	0.0
33	5.780179763590648E-4	0.0	0.0	5.00592468425768	0.0
34	5.780179763590648E-4	0.0	0.0	5.191179445680761	0.0
35	5.780179763590648E-4	0.0	0.0	5.378457270021098	0.0
36	5.780179763590648E-4	0.0	0.0	5.579029507817693	0.0
37	5.780179763590648E-4	0.0	0.0	5.757059044536285	0.0
38	5.780179763590648E-4	0.0	0.0	5.96398948007283	0.0
39	5.780179763590648E-4	0.0	0.0	6.177856131325684	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	20	7.0264435E-4	45.000004	3
TCTTCGT	20	7.0264435E-4	45.000004	41
TTCGTCG	20	7.0264435E-4	45.000004	34
TGTGCGT	20	7.0264435E-4	45.000004	9
CGTTAAG	20	7.0264435E-4	45.000004	2
CGTAGTG	20	7.0264435E-4	45.000004	2
CGCTAGG	20	7.0264435E-4	45.000004	3
GAAACGT	20	7.0264435E-4	45.000004	41
GACGGGT	35	1.2086457E-7	45.000004	4
CTATTAG	20	7.0264435E-4	45.000004	2
CCGCGAA	20	7.0264435E-4	45.000004	26
TATGCGG	25	3.885136E-5	45.0	2
TTGCGCG	25	3.885136E-5	45.0	14
TAAGGTC	25	3.885136E-5	45.0	5
CGGTCTA	25	3.885136E-5	45.0	31
CGGACAG	30	2.1610886E-6	44.999996	2
TTGAGCG	30	2.1610886E-6	44.999996	1
ATCGCGG	30	2.1610886E-6	44.999996	2
CGTTTTA	545	0.0	43.34862	1
CGGTTTT	115	0.0	43.04348	1
>>END_MODULE