##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936143.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505145
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.871401280820358	31.0	31.0	34.0	30.0	34.0
2	32.061449682764355	33.0	31.0	34.0	30.0	34.0
3	31.88395609181522	33.0	31.0	34.0	30.0	34.0
4	35.69212206396183	37.0	35.0	37.0	35.0	37.0
5	32.02269447386394	37.0	35.0	37.0	0.0	37.0
6	33.90428094903444	37.0	35.0	37.0	19.0	37.0
7	20.809516079541517	32.0	0.0	37.0	0.0	37.0
8	28.101921230537766	35.0	17.0	37.0	17.0	37.0
9	34.75182373377941	37.0	32.0	39.0	31.0	39.0
10	36.14149006720842	37.0	35.0	39.0	32.0	39.0
11	36.58092626869513	38.0	35.0	39.0	32.0	39.0
12	37.01865207019767	39.0	37.0	39.0	34.0	39.0
13	36.86897425491691	39.0	37.0	39.0	33.0	39.0
14	38.265915727167446	40.0	38.0	41.0	34.0	41.0
15	38.42696057567629	40.0	38.0	41.0	34.0	41.0
16	38.47092814934326	40.0	38.0	41.0	34.0	41.0
17	38.436064892258656	40.0	38.0	41.0	34.0	41.0
18	38.3257005414287	40.0	38.0	41.0	34.0	41.0
19	38.28475982143741	40.0	37.0	41.0	34.0	41.0
20	38.26550594383791	40.0	37.0	41.0	34.0	41.0
21	38.17962367241089	40.0	37.0	41.0	34.0	41.0
22	38.14690039493611	40.0	37.0	41.0	34.0	41.0
23	38.09111641211929	40.0	37.0	41.0	34.0	41.0
24	37.96983836324224	40.0	37.0	41.0	33.0	41.0
25	37.83543537004226	40.0	36.0	41.0	33.0	41.0
26	37.7046075879203	40.0	36.0	41.0	33.0	41.0
27	37.677017490027616	40.0	36.0	41.0	33.0	41.0
28	37.58370368903978	40.0	36.0	41.0	33.0	41.0
29	37.55349454117135	40.0	36.0	41.0	33.0	41.0
30	37.422476714606695	40.0	36.0	41.0	33.0	41.0
31	37.32278454701125	40.0	36.0	41.0	32.0	41.0
32	37.20869057399361	40.0	36.0	41.0	32.0	41.0
33	37.06173474942838	39.0	36.0	41.0	31.0	41.0
34	36.92110780073048	39.0	35.0	41.0	31.0	41.0
35	36.771560641004065	39.0	35.0	41.0	30.0	41.0
36	36.61747221094933	39.0	35.0	41.0	30.0	41.0
37	36.535060230230926	39.0	35.0	41.0	30.0	41.0
38	36.406509022161956	39.0	35.0	41.0	30.0	41.0
39	36.35818428372052	39.0	35.0	41.0	30.0	41.0
40	36.28780449177959	39.0	35.0	41.0	30.0	41.0
41	36.19196666303735	39.0	35.0	40.0	29.0	41.0
42	36.11178572489087	39.0	35.0	40.0	29.0	41.0
43	36.02612913123954	39.0	35.0	40.0	28.0	41.0
44	35.89028892694177	39.0	35.0	40.0	28.0	41.0
45	35.782246681645866	39.0	35.0	40.0	27.0	41.0
46	35.66988092527888	39.0	35.0	40.0	27.0	41.0
47	35.63857506260579	39.0	35.0	40.0	27.0	41.0
48	35.505282641617754	38.0	35.0	40.0	26.0	41.0
49	35.37910105019351	38.0	34.0	40.0	26.0	41.0
50	35.20752259252294	38.0	34.0	40.0	26.0	41.0
51	33.31861940630908	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	24.0
10	26.0
11	11.0
12	13.0
13	16.0
14	15.0
15	27.0
16	41.0
17	62.0
18	138.0
19	282.0
20	508.0
21	893.0
22	1374.0
23	2146.0
24	3232.0
25	4579.0
26	5740.0
27	6500.0
28	7063.0
29	7957.0
30	10021.0
31	12971.0
32	17036.0
33	24351.0
34	33611.0
35	44084.0
36	58268.0
37	96584.0
38	101434.0
39	66130.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.95675499114116	20.455116847637807	24.644211068109158	13.94391709311188
2	32.96281265775173	24.55433588375615	25.852181056924252	16.63067040156787
3	30.92161656554059	23.935107741341596	29.353156024507815	15.790119668610004
4	27.920300111849073	25.904641241623693	28.199625850003464	17.975432796523773
5	22.74376664126142	37.53714280058201	23.346761820863314	16.372328737293252
6	25.347969394926213	35.57374615209494	25.520395134070416	13.557889318908432
7	47.764305298478654	43.30954478417088	5.9206762414752205	3.0054736758752436
8	82.61707034613823	5.391917172297063	8.729374733987271	3.2616377475774283
9	76.27592077522296	8.334042700610715	10.507873976778944	4.8821625473873835
10	38.193587979689	31.015253046155067	17.89149650100466	12.899662473151274
11	28.60663769808669	27.474487523384372	26.860406417959197	17.058468360569737
12	26.98947826861594	23.788615150105414	30.89786101020499	18.32404557107365
13	23.150382563422383	25.1444634708846	31.566579892902034	20.13857407279098
14	19.367706302150868	29.273574914133565	30.686238604757047	20.672480178958516
15	19.34573241346544	27.738966039454017	34.379831533520075	18.535470013560463
16	21.59815498520227	25.49347217135674	33.86908709380475	19.039285749636242
17	22.224905720139763	25.327579209929823	30.801848974056956	21.645666095873462
18	22.247671460669707	26.398756792604104	30.572607865068445	20.78096388165774
19	23.711409595264726	27.120133822961723	29.360876579991885	19.807580001781666
20	25.76507735402706	25.225034396064494	29.906858426788347	19.103029823120092
21	24.40487384810302	26.729552900652287	29.249819358797968	19.615753892446726
22	22.451771273594712	26.38133605202467	28.912688435993626	22.254204238386997
23	22.410594977679676	27.699175484266892	29.52182046739055	20.368409070662878
24	21.900642389808866	25.670649021568064	31.41889952389908	21.00980906472399
25	21.786615724198004	27.149630304170092	29.232794544140788	21.830959427491116
26	21.115917211889656	28.45222658840531	29.338308802423068	21.09354739728197
27	22.124934424769126	28.906947510120855	29.24744380326441	19.72067426184561
28	20.900137584257987	27.904067149036415	31.3470389690089	19.848756297696703
29	21.343178691266864	26.910886973047344	30.116105276702733	21.629829058983063
30	21.774342020607946	26.70500549347217	30.53440101357036	20.986251472349522
31	22.797216640766514	27.92663492660523	28.508646032327352	20.767502400300902
32	23.650437003236693	27.55228696710845	28.337408070949927	20.459867958704926
33	22.986667194567897	27.85853566797652	28.644646586623644	20.51015055083194
34	21.74722109493314	27.071236971562623	29.766700650308326	21.414841283195916
35	22.732680715438143	25.571073652119686	29.211810470261014	22.484435162181157
36	22.16947609102337	28.130734739530233	28.96099139850934	20.738797770937055
37	22.66260182719813	28.46964732898475	29.803323798117372	19.06442704569975
38	22.424848310881035	27.50675548604856	29.164497322550954	20.903898880519456
39	21.95132090785814	26.696691049104714	30.508665828623467	20.843322214413686
40	22.743370715339157	26.125963832167002	30.904789713844536	20.2258757386493
41	20.651892031000997	27.30186382127904	30.827188233081593	21.21905591463837
42	21.778499242791675	27.263459006819822	29.929030278434904	21.0290114719536
43	23.142266082016054	26.664621049401653	28.81449880727316	21.37861406130913
44	22.329430163616387	27.277712340021182	28.517950291500462	21.87490720486197
45	21.66467054014194	26.379158459452235	29.237941581130173	22.718229419275655
46	21.311306654524937	28.29346029357907	28.729572696948402	21.665660354947587
47	21.90499757495373	26.327292163636184	31.263300636450918	20.50440962495917
48	22.412970533213237	25.55741420780172	30.345346385691236	21.684268873293806
49	20.818774807233567	26.692533826920982	30.32614397846163	22.16254738738382
50	20.13778222094646	27.07103900860149	30.24596897920399	22.54520979124806
51	20.191826109334944	26.478931791861744	29.437488245949183	23.89175385285413
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	221.0
1	632.5
2	1044.0
3	3782.0
4	6520.0
5	4523.0
6	2526.0
7	2270.0
8	2014.0
9	1907.0
10	1800.0
11	1855.5
12	1911.0
13	1827.5
14	1744.0
15	1723.5
16	1703.0
17	1695.5
18	1688.0
19	1920.0
20	2152.0
21	2533.5
22	2915.0
23	3204.0
24	3493.0
25	4272.5
26	5805.5
27	6559.0
28	8259.0
29	9959.0
30	11560.0
31	13161.0
32	14348.5
33	15536.0
34	17094.5
35	18653.0
36	19881.0
37	21109.0
38	22821.0
39	24533.0
40	26895.0
41	29257.0
42	30897.5
43	32538.0
44	34523.0
45	36508.0
46	38170.0
47	39832.0
48	39249.5
49	38667.0
50	36438.5
51	34210.0
52	31157.5
53	28105.0
54	26172.5
55	24240.0
56	22890.0
57	21540.0
58	20897.5
59	20255.0
60	19310.0
61	18365.0
62	16122.0
63	13879.0
64	11133.5
65	8388.0
66	6946.5
67	5505.0
68	4567.0
69	3629.0
70	2998.5
71	2368.0
72	1945.0
73	1522.0
74	1212.0
75	702.0
76	502.0
77	400.5
78	299.0
79	217.0
80	135.0
81	108.0
82	81.0
83	68.0
84	55.0
85	44.5
86	34.0
87	26.0
88	18.0
89	12.5
90	7.0
91	5.5
92	4.0
93	3.0
94	2.0
95	2.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	505145.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.15384864838688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.86085862829526	27.799221724954908
2	7.955474401818212	6.070639325061763
3	3.7433340931323706	4.2846780728915705
4	2.440498676558252	3.7245766852796804
5	1.8465050246722874	3.5225636619916165
6	1.4766084483439266	3.380297715062614
7	1.2433606251048586	3.3207295173930125
8	1.0408694366532605	3.1770539959040174
9	0.968235444520656	3.3247717745620227
>10	6.368363362941748	38.473956926625355
>50	0.03406741818463364	0.8741175963558309
>100	0.020759832956256018	1.3227527660465728
>500	0.0	0.0
>1k	0.0010646068182695394	0.7246402378710535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2267	0.44878203288164786	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1301	0.2575498124300943	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04572944402102366	0.0
2	0.0	0.0	0.0	0.22132259054330936	0.0
3	0.0	0.0	0.0	0.303279256451118	0.0
4	0.0	0.0	0.0	0.38444407051440677	0.0
5	0.0	0.0	0.0	0.6760435122588564	0.0
6	0.0	0.0	0.0	0.8823209177562878	0.0
7	0.0	0.0	0.0	1.112353878589316	0.0
8	0.0	0.0	0.0	1.6715992437814884	0.0
9	0.0	0.0	0.0	1.898266834275307	0.0
10	0.0	0.0	0.0	2.531946272852349	0.0
11	0.0	0.0	0.0	3.1644379336626116	0.0
12	0.0	0.0	0.0	3.739916261667442	0.0
13	0.0	0.0	0.0	3.9871720001187776	0.0
14	0.0	0.0	0.0	4.098031258351562	0.0
15	0.0	0.0	0.0	4.282532738124697	0.0
16	0.0	0.0	0.0	4.6564847716992155	0.0
17	0.0	0.0	0.0	5.084678656623346	0.0
18	0.0	0.0	0.0	5.604331429589524	0.0
19	1.9796296112997258E-4	0.0	0.0	5.883459204782785	0.0
20	1.9796296112997258E-4	0.0	0.0	6.166150313276386	0.0
21	1.9796296112997258E-4	0.0	0.0	6.521691791465817	0.0
22	1.9796296112997258E-4	0.0	0.0	6.914054380425422	0.0
23	1.9796296112997258E-4	0.0	0.0	7.304635302734859	0.007918518445198903
24	1.9796296112997258E-4	0.0	0.0	7.621376040542814	0.007918518445198903
25	1.9796296112997258E-4	0.0	0.0	7.897138445396866	0.007918518445198903
26	1.9796296112997258E-4	0.0	0.0	8.173494739134307	0.007918518445198903
27	1.9796296112997258E-4	0.0	0.0	8.438171218165081	0.007918518445198903
28	1.9796296112997258E-4	0.0	0.0	8.717298993358343	0.007918518445198903
29	1.9796296112997258E-4	0.0	0.0	9.013451583208782	0.007918518445198903
30	1.9796296112997258E-4	0.0	0.0	9.346227320868266	0.007918518445198903
31	1.9796296112997258E-4	0.0	0.0	9.661780280909442	0.007918518445198903
32	1.9796296112997258E-4	0.0	0.0	9.972582129883499	0.007918518445198903
33	3.9592592225994516E-4	0.0	0.0	10.25626305318275	0.007918518445198903
34	3.9592592225994516E-4	0.0	0.0	10.55023805046076	0.007918518445198903
35	5.938888833899177E-4	0.0	0.0	10.881232121470074	0.007918518445198903
36	5.938888833899177E-4	0.0	0.0	11.213611933207297	0.007918518445198903
37	5.938888833899177E-4	0.0	0.0	11.535499708004632	0.007918518445198903
38	5.938888833899177E-4	0.0	0.0	11.8476872977066	0.007918518445198903
39	5.938888833899177E-4	0.0	0.0	12.170960813231844	0.007918518445198903
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCCGT	20	7.029527E-4	45.000004	43
ATAGGCG	45	3.8380676E-10	45.000004	5
CGCATCG	20	7.029527E-4	45.000004	21
GTTACGG	40	6.8012014E-9	45.000004	2
TGTAACG	20	7.029527E-4	45.000004	1
TAACGCC	25	3.8876904E-5	45.0	12
TCGTGCG	55	1.8189894E-12	45.0	45
TCCGGTA	25	3.8876904E-5	45.0	18
CGTTTTA	505	0.0	43.217823	1
CGTTATT	190	0.0	42.63158	1
TTACGGG	140	0.0	41.785713	3
CGAATAT	105	0.0	40.714287	14
GACCGAT	200	0.0	40.5	9
ACGATAG	40	3.4530967E-7	39.375004	2
TACGAAT	110	0.0	38.863636	12
GCTACGA	105	0.0	38.57143	10
TAACGAA	35	6.2413365E-6	38.571426	1
TGAGCGT	35	6.2413365E-6	38.571426	16
CGTTTTT	870	0.0	38.275864	1
AGGCGAT	265	0.0	38.207546	7
>>END_MODULE