##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936140.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	419252
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0397445927509	33.0	31.0	34.0	30.0	34.0
2	32.233055537004	33.0	31.0	34.0	30.0	34.0
3	32.08038125041741	33.0	31.0	34.0	30.0	34.0
4	35.846309618081726	37.0	35.0	37.0	35.0	37.0
5	32.03041368914161	37.0	35.0	37.0	0.0	37.0
6	33.951861887361304	37.0	35.0	37.0	18.0	37.0
7	20.829899439954968	32.0	0.0	37.0	0.0	37.0
8	28.121838416990258	35.0	17.0	37.0	17.0	37.0
9	34.85030005819889	37.0	32.0	39.0	32.0	39.0
10	36.316339576197606	37.0	35.0	39.0	32.0	39.0
11	36.696960300726055	38.0	35.0	39.0	33.0	39.0
12	37.03555141060746	39.0	37.0	39.0	34.0	39.0
13	36.77092536231193	39.0	37.0	39.0	33.0	39.0
14	38.06798297921059	40.0	37.0	41.0	33.0	41.0
15	38.276044002175304	40.0	38.0	41.0	33.0	41.0
16	38.39504641599802	40.0	38.0	41.0	34.0	41.0
17	38.3650501369105	40.0	37.0	41.0	34.0	41.0
18	38.27468920839972	40.0	37.0	41.0	34.0	41.0
19	38.218832587560705	40.0	37.0	41.0	34.0	41.0
20	38.18388940303207	40.0	37.0	41.0	34.0	41.0
21	38.0849823018137	40.0	37.0	41.0	34.0	41.0
22	38.07210222014445	40.0	37.0	41.0	34.0	41.0
23	38.03218350777098	40.0	36.0	41.0	34.0	41.0
24	37.90488775247345	40.0	36.0	41.0	33.0	41.0
25	37.73131195557803	40.0	36.0	41.0	33.0	41.0
26	37.60373713184433	40.0	36.0	41.0	33.0	41.0
27	37.542136948660946	40.0	36.0	41.0	33.0	41.0
28	37.43574031847194	40.0	35.0	41.0	33.0	41.0
29	37.38302023603942	40.0	35.0	41.0	33.0	41.0
30	37.21424107696564	39.0	35.0	41.0	32.0	41.0
31	37.08008310037877	39.0	35.0	41.0	32.0	41.0
32	36.94719882075697	39.0	35.0	41.0	31.0	41.0
33	36.80955368131816	39.0	35.0	41.0	31.0	41.0
34	36.61688674114852	39.0	35.0	41.0	30.0	41.0
35	36.45960901796533	39.0	35.0	41.0	30.0	41.0
36	36.262808525659985	39.0	35.0	41.0	30.0	41.0
37	36.15577027658783	39.0	35.0	41.0	29.0	41.0
38	35.9733811645502	39.0	35.0	40.0	29.0	41.0
39	35.85729823590585	39.0	35.0	40.0	28.0	41.0
40	35.745365555799374	39.0	35.0	40.0	27.0	41.0
41	35.62088910726723	39.0	35.0	40.0	27.0	41.0
42	35.509163939587644	38.0	35.0	40.0	26.0	41.0
43	35.417424365298196	38.0	34.0	40.0	26.0	41.0
44	35.246691727171246	38.0	34.0	40.0	26.0	41.0
45	35.149563985383494	38.0	34.0	40.0	25.0	41.0
46	35.0457576827302	38.0	34.0	40.0	25.0	41.0
47	34.93988341140889	38.0	34.0	40.0	24.0	41.0
48	34.843857632163946	38.0	34.0	40.0	24.0	41.0
49	34.71621363762129	38.0	34.0	40.0	24.0	41.0
50	34.597394884222375	37.0	34.0	40.0	24.0	41.0
51	32.71894707717554	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	19.0
9	35.0
10	41.0
11	16.0
12	15.0
13	11.0
14	21.0
15	34.0
16	46.0
17	87.0
18	171.0
19	300.0
20	537.0
21	936.0
22	1526.0
23	2295.0
24	3212.0
25	4289.0
26	5341.0
27	5897.0
28	6314.0
29	7248.0
30	8648.0
31	10923.0
32	14641.0
33	21697.0
34	29732.0
35	38756.0
36	48440.0
37	78541.0
38	79244.0
39	50227.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3596118802057	20.40371900432198	25.233272590232126	13.003396525240191
2	33.3353210002576	24.186408174558498	25.300773758980277	17.17749706620362
3	32.26412754143093	23.71747779378512	27.70028526995697	16.318109394826976
4	27.36230238615439	25.931420720712122	27.889193134439434	18.817083758694057
5	22.584984687014014	36.756413803631226	23.715331113506913	16.943270395847843
6	26.544178680125558	33.74581397345749	25.477278581855305	14.232728764561648
7	48.28766469808134	43.047379618940404	5.9253146079207735	2.7396410750574836
8	83.47891005886675	4.792821501149667	8.50133094177249	3.2269374982111
9	77.67595622680392	6.89489853357885	10.425710551172088	5.003434688445135
10	44.193945407535324	25.089922051653897	17.333489166420197	13.382643374390582
11	32.51314245370327	25.545972350758017	25.16481734136033	16.776067854178393
12	29.322460000190816	23.85414977149781	28.47475981032887	18.348630417982502
13	24.993082919103546	25.566723593447378	29.9733811645502	19.46681232289888
14	20.864301184013435	26.939167851316153	31.582437293083878	20.614093671586538
15	20.794176294925247	24.521767338021046	34.30156564548291	20.382490721570797
16	25.079427170293762	23.83888448951943	30.919113087117054	20.162575253069754
17	25.48014082222625	24.18211481400208	29.16241305944873	21.175331304322935
18	25.667617566523237	23.611097859998285	29.71840325150506	21.00288132197342
19	25.923788079722936	25.042933605564194	28.104099682291316	20.92917863242155
20	27.92497113907626	25.17769742302959	28.023241391812086	18.87409004608207
21	25.62134468052627	25.54978867125261	29.106599372215282	19.72226727600584
22	25.963620924885273	22.578783166210297	29.253766231288104	22.203829677616326
23	23.641628423955044	25.04317212559511	30.294667646188927	21.020531804260923
24	23.30316850009064	24.179729613692956	31.136166315247156	21.38093557096925
25	24.00083959050881	26.02754429316974	29.13331361567744	20.838302500644005
26	23.11115987520632	26.655329014530636	27.452939997901023	22.780571112362015
27	22.123209907168004	26.438514306431454	30.138675545972347	21.29960024042819
28	21.845334071155296	26.540839399692786	30.163004589125396	21.450821940026525
29	22.921536450631123	25.446986537929455	29.559071870855714	22.072405140583705
30	23.005256981481303	25.793317622813966	30.14606966693063	21.055355728774103
31	24.53822522015399	25.961474244607064	28.646732752616565	20.853567782622385
32	25.095646532395794	26.014187171438657	28.478099090761646	20.41206720540391
33	25.076087889860993	25.581750355394846	27.815013404825738	21.527148349918427
34	22.864053123181286	26.017287931840517	29.57290603264862	21.545752912329576
35	23.588199937030712	26.50744659536508	29.213217826033027	20.69113564157118
36	24.84687014015437	28.37863623787126	26.186875673819088	20.587617948155287
37	23.595117017927166	27.757530077375897	27.7477507561085	20.89960214858844
38	23.38354975050805	27.81000448417658	27.530697527978397	21.275748237336973
39	22.91461936973467	27.36564166658716	27.29575529752989	22.423983666148285
40	24.676805358113974	26.450440307977065	27.896348735366793	20.976405598542165
41	22.51247459761671	26.0678541783939	28.062835716943507	23.35683550704588
42	22.776039231774682	25.968152805472606	27.79235400188908	23.463453960863635
43	22.29995325007394	26.21430547737399	28.529380897407762	22.956360375144307
44	22.05332353811073	25.783776821577476	28.828962056233483	23.33393758407831
45	21.851535591959014	26.8833541640827	27.875120452615608	23.38998979134268
46	22.812771316535162	27.498735843836165	26.899812046215644	22.78868079341303
47	21.44724413956284	27.128314235829524	29.393061929340824	22.03137969526681
48	22.40752578401534	27.29193897703529	29.122103174224574	21.178432064724795
49	22.742169387385154	27.18221976281568	28.65698911394579	21.418621735853378
50	21.654279526394625	27.042924064762957	28.871418621735856	22.43137778710656
51	21.639014244416245	26.822770076231002	27.800225162909182	23.73799051644357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	172.0
1	542.5
2	913.0
3	2981.5
4	5050.0
5	3441.0
6	1832.0
7	1628.0
8	1424.0
9	1403.0
10	1382.0
11	1427.5
12	1473.0
13	1465.5
14	1458.0
15	1382.5
16	1307.0
17	1421.5
18	1536.0
19	1582.5
20	1629.0
21	1852.5
22	2076.0
23	2452.5
24	2829.0
25	3237.0
26	4192.0
27	4739.0
28	5734.5
29	6730.0
30	7892.5
31	9055.0
32	9928.5
33	10802.0
34	12136.0
35	13470.0
36	14757.5
37	16045.0
38	17092.0
39	18139.0
40	19731.0
41	21323.0
42	22862.0
43	24401.0
44	25477.0
45	26553.0
46	27170.5
47	27788.0
48	27801.5
49	27815.0
50	27146.0
51	26477.0
52	25338.0
53	24199.0
54	23607.5
55	23016.0
56	22509.5
57	22003.0
58	21309.0
59	20615.0
60	19323.0
61	18031.0
62	16539.5
63	15048.0
64	13193.0
65	11338.0
66	10092.0
67	8846.0
68	7359.5
69	5873.0
70	4947.5
71	4022.0
72	3222.5
73	2423.0
74	2059.0
75	1327.5
76	960.0
77	720.5
78	481.0
79	405.5
80	330.0
81	245.5
82	161.0
83	118.0
84	75.0
85	54.5
86	34.0
87	28.5
88	23.0
89	15.5
90	8.0
91	5.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	419252.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.29014601704154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03703356765315	23.315430740676135
2	7.299039047679587	4.859721513626827
3	3.8598416141076433	3.8548407280889028
4	2.7583893602961114	3.6730873830444546
5	2.1485332109808524	3.5762492158007855
6	1.7589355276790164	3.5133132330597885
7	1.3784468400896146	3.212208760831895
8	1.3087661451366106	3.4855212859006683
9	1.1166613733394486	3.345643815305435
>10	8.289490945389675	45.322673462465985
>50	0.03690291532355135	0.8098487380103883
>100	0.0065122791747443546	0.3950481648831162
>500	0.0	0.0
>1k	0.00144717314994319	0.6364129583056054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1638	0.3906958106341771	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1004	0.2394741110358448	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017411962256590308	0.0
2	0.0	0.0	0.0	0.09970137292129794	0.0
3	0.0	0.0	0.0	0.13500233749630294	0.0
4	0.0	0.0	0.0	0.178890023184147	0.0
5	0.0	0.0	0.0	0.3398910440498793	0.0
6	0.0	0.0	0.0	0.44483985765124556	0.0
7	0.0	0.0	0.0	0.5552746319635923	0.0
8	0.0	0.0	0.0	0.8271874672034958	0.0
9	0.0	0.0	0.0	0.9404844818867889	0.0
10	0.0	0.0	0.0	1.2837148063694388	0.0
11	0.0	0.0	0.0	1.5563432017020788	0.0
12	0.0	0.0	0.0	1.8616488412696899	0.0
13	0.0	0.0	0.0	1.9692213752110903	0.0
14	0.0	0.0	0.0	2.0243195023518075	0.0
15	0.0	0.0	0.0	2.116388234283915	0.0
16	0.0	0.0	0.0	2.2888382166334327	0.0
17	0.0	0.0	0.0	2.4791772013013653	0.0
18	0.0	0.0	0.0	2.6850199879785905	0.0
19	0.0	0.0	0.0	2.815967484949386	0.0
20	0.0	0.0	0.0	2.9550246629711965	0.0
21	0.0	0.0	0.0	3.1291442855370994	0.0
22	0.0	0.0	0.0	3.3070802285975978	0.0
23	0.0	0.0	0.0	3.483823571503535	0.0
24	0.0	0.0	0.0	3.6355223111636916	0.0
25	0.0	0.0	0.0	3.77219428887638	0.0
26	2.38520030912196E-4	0.0	0.0	3.8957476648888973	0.0
27	2.38520030912196E-4	0.0	0.0	4.041721923807161	0.0
28	2.38520030912196E-4	0.0	0.0	4.194851783652791	0.0
29	2.38520030912196E-4	0.0	0.0	4.363008405445889	0.0
30	2.38520030912196E-4	0.0	0.0	4.572190472555885	0.0
31	2.38520030912196E-4	0.0	0.0	4.746071575090876	0.0
32	2.38520030912196E-4	0.0	0.0	4.924007518151375	0.0
33	2.38520030912196E-4	0.0	0.0	5.1017049411809605	0.0
34	2.38520030912196E-4	0.0	0.0	5.284888324921527	0.0
35	2.38520030912196E-4	0.0	0.0	5.497409672464293	0.0
36	2.38520030912196E-4	0.0	0.0	5.684886416761279	0.0
37	2.38520030912196E-4	0.0	0.0	5.876179481552861	0.0
38	2.38520030912196E-4	0.0	0.0	6.1084979916613396	0.0
39	2.38520030912196E-4	0.0	0.0	6.4829744401934875	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	30	2.1621909E-6	45.000004	9
ATAACGG	30	2.1621909E-6	45.000004	2
CTTACGG	30	2.1621909E-6	45.000004	2
ACGTTGA	20	7.0281554E-4	45.0	42
CGCATTC	20	7.0281554E-4	45.0	27
CACGAGG	55	1.8189894E-12	45.0	3
TACGATA	25	3.8865517E-5	45.0	33
ATTTGCG	20	7.0281554E-4	45.0	12
GCGTACG	20	7.0281554E-4	45.0	1
GCGTACA	20	7.0281554E-4	45.0	1
ATTCTCG	20	7.0281554E-4	45.0	30
AGGACCG	120	0.0	41.250004	7
CGTTTTA	390	0.0	40.961536	1
CGTTTTT	595	0.0	40.840336	1
TGTACGG	50	1.0786607E-9	40.5	2
AGACACG	85	0.0	39.705883	24
CAGACGA	40	3.4512232E-7	39.375	1
CGGGTAC	35	6.2387953E-6	38.57143	6
GCGCAAA	35	6.2387953E-6	38.57143	1
GTCCGTC	35	6.2387953E-6	38.57143	45
>>END_MODULE