##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936139.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	419365
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.638474836955872	31.0	31.0	34.0	30.0	34.0
2	31.82397434216017	31.0	31.0	34.0	30.0	34.0
3	31.5848747511118	31.0	31.0	34.0	30.0	34.0
4	35.468751564865926	37.0	35.0	37.0	33.0	37.0
5	31.87813479904141	37.0	35.0	37.0	10.0	37.0
6	33.76501377082017	37.0	35.0	37.0	19.0	37.0
7	20.94625684069963	32.0	0.0	37.0	0.0	37.0
8	28.12183658626733	35.0	17.0	37.0	17.0	37.0
9	34.64003433762951	35.0	32.0	39.0	28.0	39.0
10	35.9146900671253	37.0	35.0	39.0	32.0	39.0
11	36.328623037211024	37.0	35.0	39.0	32.0	39.0
12	36.820042206669605	39.0	35.0	39.0	33.0	39.0
13	36.64997317372694	39.0	35.0	39.0	32.0	39.0
14	38.0328520501234	40.0	37.0	41.0	33.0	41.0
15	38.17768292537527	40.0	37.0	41.0	33.0	41.0
16	38.234895615990844	40.0	37.0	41.0	34.0	41.0
17	38.19765836443194	40.0	37.0	41.0	33.0	41.0
18	38.052023893267204	40.0	37.0	41.0	33.0	41.0
19	38.005494020721805	40.0	37.0	41.0	34.0	41.0
20	37.96573629177447	40.0	37.0	41.0	34.0	41.0
21	37.91724869743541	40.0	36.0	41.0	33.0	41.0
22	37.89621689935974	39.0	36.0	41.0	33.0	41.0
23	37.85470652057277	39.0	36.0	41.0	33.0	41.0
24	37.6940755666305	39.0	36.0	41.0	33.0	41.0
25	37.553820657422534	39.0	36.0	41.0	33.0	41.0
26	37.3922954943784	39.0	36.0	41.0	33.0	41.0
27	37.38745961155556	39.0	36.0	41.0	33.0	41.0
28	37.235990127931515	39.0	36.0	41.0	32.0	41.0
29	37.285586541556874	39.0	36.0	41.0	32.0	41.0
30	37.163869183169794	39.0	36.0	41.0	32.0	41.0
31	37.008477102285596	39.0	35.0	40.0	31.0	41.0
32	36.92359400522218	39.0	35.0	40.0	31.0	41.0
33	36.69480285670001	39.0	35.0	40.0	30.0	41.0
34	36.5225924910281	39.0	35.0	40.0	30.0	41.0
35	36.357154268954254	39.0	35.0	40.0	30.0	41.0
36	36.17411324264066	39.0	35.0	40.0	29.0	41.0
37	36.10318934579662	39.0	35.0	40.0	29.0	41.0
38	36.039495427610795	39.0	35.0	40.0	29.0	41.0
39	35.91737507898847	39.0	35.0	40.0	28.0	41.0
40	35.85935163878722	39.0	35.0	40.0	27.0	41.0
41	35.70299619663062	39.0	35.0	40.0	27.0	41.0
42	35.612304317241545	39.0	35.0	40.0	26.0	41.0
43	35.50748154948553	38.0	35.0	40.0	26.0	41.0
44	35.38938394954276	38.0	35.0	40.0	25.0	41.0
45	35.331699116521406	38.0	35.0	40.0	24.0	41.0
46	35.13358053247171	38.0	34.0	40.0	24.0	41.0
47	35.07047798457191	38.0	34.0	40.0	24.0	41.0
48	34.9339572925733	38.0	34.0	40.0	23.0	41.0
49	34.759572210365675	38.0	34.0	40.0	23.0	41.0
50	34.55661774349314	38.0	34.0	40.0	22.0	41.0
51	32.26801235200839	36.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	10.0
10	12.0
11	9.0
12	6.0
13	5.0
14	2.0
15	9.0
16	22.0
17	41.0
18	113.0
19	244.0
20	422.0
21	767.0
22	1309.0
23	2250.0
24	3456.0
25	4906.0
26	5854.0
27	6380.0
28	7050.0
29	8081.0
30	9939.0
31	12130.0
32	16095.0
33	22048.0
34	29846.0
35	38425.0
36	48378.0
37	75798.0
38	76316.0
39	49423.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.150608658328665	22.0504810844968	26.227033729567324	15.571876527607214
2	30.894805241257618	26.406114005699095	26.400152611686718	16.298928141356576
3	27.36184469376319	26.518903580413244	30.32751898703993	15.791732738783637
4	25.25985716499946	24.45292287148427	33.6952296925113	16.59199027100497
5	21.375412826535356	35.52585456583167	26.023630965865056	17.075101641767912
6	23.067733358768614	33.058791267750046	29.989627174418466	13.883848199062868
7	46.68272268787334	41.84135538254266	8.48067912200589	2.995242807578124
8	78.60670299142751	5.254849594029068	11.624241412611926	4.514206001931491
9	73.63966950031595	6.488619698830375	12.673923670311066	7.197787130542606
10	35.79530957519106	30.47631538158883	21.354905631132784	12.373469412087323
11	23.79788489740441	25.13371406769759	32.55398042278206	18.514420612115938
12	23.020280662430103	22.225507612700156	35.41950329665089	19.334708428218857
13	19.240041491302325	25.163997949280457	37.45281556639205	18.14314499302517
14	17.113493019207613	28.72438090923182	33.26958616002766	20.89253991153291
15	15.49437840544633	26.56564090947027	40.572770736709074	17.36720994837433
16	17.64048024990164	26.334100366029595	34.84458645809736	21.18083292597141
17	18.46196034480703	25.31184052078738	33.52950293896725	22.69669619543834
18	19.270802284406187	25.952809604998034	34.954753019446066	19.821635091149716
19	19.080514587531148	27.470103609027934	32.13596747463427	21.313414328806648
20	19.765836443193876	27.759111990747915	33.80396551929703	18.67108604676117
21	19.210234521240448	28.35191301133857	33.68116080264209	18.75669166477889
22	18.023678657017157	25.32781705674055	32.21680397744209	24.43170030880021
23	16.573152265925863	28.30779869564699	33.79561956767971	21.323429470747442
24	19.035923360318577	25.593933685453006	33.67162257222229	21.69852038200613
25	16.63777377702002	29.721602899622045	31.718431438007467	21.92219188535047
26	16.582928952106162	28.14374113242641	32.83583513168719	22.43749478378024
27	19.057145923002636	27.522086964815852	32.89759517365541	20.523171938526104
28	15.363227737174059	26.802189023881347	35.24066147627961	22.59392176266498
29	17.616873129612628	23.788585122745104	33.8964863543691	24.69805539327316
30	17.93759612747845	25.958532543249913	34.248923968380765	21.85494736089087
31	17.87130542606083	25.507851155914302	31.926126405398637	24.69471701262623
32	18.374208624944856	26.327423604735735	32.90880259439867	22.389565175920737
33	17.52888295398996	26.219403145231478	31.240089182454422	25.011624718324132
34	16.234068174501925	24.979910102178295	34.222693834726314	24.56332788859347
35	15.992989400641447	24.496321819894366	33.1379585802344	26.37273019922979
36	17.32571864604819	27.574547232124758	31.11823828884146	23.981495832985587
37	15.893791804275512	26.218449322189503	36.85119168266308	21.036567190871914
38	16.430078809628842	26.777151169029366	30.920081551870087	25.8726884694717
39	18.5354047190395	25.743445447283392	34.004506813873355	21.71664301980375
40	18.689924051840283	24.600526987230694	32.15051327602447	24.559035684904558
41	16.235975820585885	27.571447307238323	31.54769711349302	24.64487975868277
42	17.989341027505873	25.71387693298201	33.050922227653714	23.245859811858406
43	20.065575334136135	26.486950508506908	30.455569730425765	22.991904426931193
44	18.57141153887425	26.2329951235797	31.061485817843643	24.134107519702408
45	18.307679467766743	25.259141797717977	30.641565223611888	25.79161351090339
46	20.082267237370786	26.478127645368595	30.016572675354404	23.423032441906216
47	16.47252393499696	24.581927437912082	35.05037377940457	23.89517484768638
48	17.82432964124331	24.937941888331167	31.530766754497876	25.70696171592765
49	17.847936761532317	23.696779654954515	34.033598416653746	24.42168516685942
50	17.70629403979827	23.529145255326505	33.473942746771904	25.290617958103322
51	17.309980565855522	23.355787917446616	30.439354738712098	28.89487677798576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	138.0
1	327.5
2	517.0
3	3407.0
4	6297.0
5	4465.0
6	2633.0
7	2332.5
8	2032.0
9	2001.5
10	1971.0
11	1909.0
12	1847.0
13	1839.0
14	1831.0
15	1799.0
16	1767.0
17	1715.5
18	1664.0
19	1688.0
20	1712.0
21	1968.0
22	2224.0
23	2563.0
24	2902.0
25	3658.0
26	5291.0
27	6168.0
28	7094.5
29	8021.0
30	9413.0
31	10805.0
32	12340.5
33	13876.0
34	14864.5
35	15853.0
36	17143.0
37	18433.0
38	20249.5
39	22066.0
40	24419.5
41	26773.0
42	29045.0
43	31317.0
44	35532.0
45	39747.0
46	45229.5
47	50712.0
48	46018.0
49	41324.0
50	37391.0
51	33458.0
52	27924.5
53	22391.0
54	18820.5
55	15250.0
56	12706.0
57	10162.0
58	8771.5
59	7381.0
60	6505.0
61	5629.0
62	4626.0
63	3623.0
64	2881.0
65	2139.0
66	1578.0
67	1017.0
68	792.0
69	567.0
70	430.5
71	294.0
72	242.5
73	191.0
74	138.5
75	61.0
76	36.0
77	31.5
78	27.0
79	25.5
80	24.0
81	18.0
82	12.0
83	13.5
84	15.0
85	11.0
86	7.0
87	7.0
88	7.0
89	4.0
90	1.0
91	2.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	419365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.94108538702839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00095754085461	33.395645647986115
2	8.963326841978645	7.877166202304544
3	3.6281578462289112	4.782755811562847
4	2.181436362910312	3.8341872595604265
5	1.498394411023959	3.2920538379124946
6	1.1417380960237782	3.010152670020243
7	0.9360588063218915	2.879200795510908
8	0.7504416376798625	2.638017606341781
9	0.6511767534098749	2.575207199126812
>10	4.191166076899675	29.788590752921095
>50	0.03516653948829437	1.0406332798681934
>100	0.01703379256464252	1.8091566510562294
>500	0.0010989543590091946	0.3510628280576214
>1k	0.003846340256532182	2.726169457770704
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2116	0.5045723892074923	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	1990	0.47452696338511796	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCG	1979	0.47190395001967256	No Hit
GAATCTATCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	1449	0.3455223969573045	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCG	1331	0.3173846172188905	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1233	0.2940159526903771	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	1193	0.2844777222705758	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	894	0.21317944988256052	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	560	0.13353522587721914	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	495	0.11803560144504192	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	488	0.11636641112157667	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	450	0.10730509222276537	No Hit
GCTGTCACTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	426	0.10158215397088455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3845576049503415E-4	0.0	0.0	0.11350494199563627	0.0
2	2.3845576049503415E-4	0.0	0.0	0.6817450192553026	0.0
3	2.3845576049503415E-4	0.0	0.0	0.8672636009204392	0.0
4	2.3845576049503415E-4	0.0	0.0	1.2061092365838828	0.0
5	2.3845576049503415E-4	0.0	0.0	2.4234258939110322	0.0
6	2.3845576049503415E-4	0.0	0.0	2.9423056287482265	0.0
7	2.3845576049503415E-4	0.0	0.0	3.6543345295863987	0.0
8	2.3845576049503415E-4	0.0	0.0	5.049539184242843	0.0
9	2.3845576049503415E-4	0.0	0.0	5.507851155914299	0.0
10	2.3845576049503415E-4	0.0	0.0	7.480357206729222	0.0
11	2.3845576049503415E-4	0.0	0.0	8.807601969644582	0.0
12	2.3845576049503415E-4	0.0	0.0	10.485138244727146	0.0
13	2.3845576049503415E-4	0.0	0.0	10.956803738986325	0.0
14	2.3845576049503415E-4	0.0	0.0	11.155437387478688	0.0
15	2.3845576049503415E-4	0.0	0.0	11.488321629129755	0.0
16	2.3845576049503415E-4	0.0	0.0	12.126667699974963	0.0
17	2.3845576049503415E-4	0.0	0.0	12.961262861707581	0.0
18	2.3845576049503415E-4	0.0	0.0	13.904832305986432	0.0
19	2.3845576049503415E-4	0.0	0.0	14.481418334863424	0.0
20	2.3845576049503415E-4	0.0	0.0	15.01031321164141	0.0
21	2.3845576049503415E-4	0.0	0.0	15.692296686657208	0.0
22	2.3845576049503415E-4	0.0	0.0	16.48110834237478	0.0
23	2.3845576049503415E-4	0.0	0.0	17.28899645893196	0.0
24	2.3845576049503415E-4	0.0	0.0	17.887758873534988	0.0
25	2.3845576049503415E-4	0.0	0.0	18.40711551989317	0.0
26	2.3845576049503415E-4	0.0	0.0	18.893326815542547	0.0
27	2.3845576049503415E-4	0.0	0.0	19.40505287756489	0.0
28	2.3845576049503415E-4	0.0	0.0	19.905094607322976	0.0
29	2.3845576049503415E-4	0.0	0.0	20.425166620962646	0.0
30	2.3845576049503415E-4	0.0	0.0	20.99149905213835	0.0
31	2.3845576049503415E-4	0.0	0.0	21.547339429852276	0.0
32	2.3845576049503415E-4	0.0	0.0	22.034027637022643	0.0
33	2.3845576049503415E-4	0.0	0.0	22.532638632217758	0.0
34	4.769115209900683E-4	0.0	0.0	23.03768793294624	0.0
35	4.769115209900683E-4	0.0	0.0	23.55585230050195	0.0
36	4.769115209900683E-4	0.0	0.0	24.01821802010182	0.0
37	4.769115209900683E-4	0.0	0.0	24.516590559536443	0.0
38	4.769115209900683E-4	0.0	0.0	24.99350208052651	0.0
39	4.769115209900683E-4	0.0	0.0	25.48519785866727	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAG	35	1.2094642E-7	45.000004	2
TATCACG	20	7.028156E-4	45.0	1
CAAACGC	25	3.886554E-5	45.0	18
CTCGTTT	20	7.028156E-4	45.0	18
GATCGTT	20	7.028156E-4	45.0	9
TTTACGG	20	7.028156E-4	45.0	2
CGCGGGT	20	7.028156E-4	45.0	4
CGAAAAT	25	3.886554E-5	45.0	35
CCAATTG	20	7.028156E-4	45.0	15
TTGTACG	25	3.886554E-5	45.0	1
TACGGGT	25	3.886554E-5	45.0	4
CGACACC	20	7.028156E-4	45.0	19
TATGCGT	20	7.028156E-4	45.0	19
GCGTTAC	20	7.028156E-4	45.0	22
TCGGCAC	20	7.028156E-4	45.0	18
CGTTACG	20	7.028156E-4	45.0	23
CCGTAGG	25	3.886554E-5	45.0	3
CGTAGGT	25	3.886554E-5	45.0	4
GCGGGTA	20	7.028156E-4	45.0	5
TTGGTCG	20	7.028156E-4	45.0	1
>>END_MODULE