##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936136.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	242594
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.787434973659696	31.0	31.0	34.0	30.0	34.0
2	31.950225479607905	33.0	31.0	34.0	30.0	34.0
3	31.64804982810787	31.0	31.0	34.0	30.0	34.0
4	35.5332366010701	37.0	35.0	37.0	33.0	37.0
5	32.035446053900756	37.0	35.0	37.0	10.0	37.0
6	33.85594862197746	37.0	35.0	37.0	19.0	37.0
7	20.67736217713546	32.0	0.0	37.0	0.0	37.0
8	27.90929289265192	35.0	17.0	37.0	17.0	37.0
9	34.521158808544314	35.0	32.0	39.0	28.0	39.0
10	36.07519971639859	37.0	35.0	39.0	32.0	39.0
11	36.55188916461248	38.0	35.0	39.0	32.0	39.0
12	36.90801503747001	39.0	37.0	39.0	33.0	39.0
13	36.65056431733679	39.0	35.0	39.0	32.0	39.0
14	38.094487085418436	40.0	37.0	41.0	33.0	41.0
15	38.21344303651368	40.0	37.0	41.0	33.0	41.0
16	38.298156590847256	40.0	37.0	41.0	34.0	41.0
17	38.20411057157226	40.0	37.0	41.0	33.0	41.0
18	38.05781264169765	40.0	37.0	41.0	33.0	41.0
19	37.963206014988	40.0	37.0	41.0	33.0	41.0
20	37.928559651104315	40.0	36.0	41.0	33.0	41.0
21	37.86289026109467	40.0	36.0	41.0	33.0	41.0
22	37.84139343924417	39.0	36.0	41.0	33.0	41.0
23	37.76521678194844	39.0	36.0	41.0	33.0	41.0
24	37.59225702202033	39.0	35.0	41.0	33.0	41.0
25	37.463131816945186	39.0	35.0	41.0	33.0	41.0
26	37.29752590748328	39.0	35.0	41.0	33.0	41.0
27	37.2533286066432	39.0	35.0	41.0	33.0	41.0
28	37.147262504431275	39.0	35.0	41.0	32.0	41.0
29	37.0867704889651	39.0	35.0	41.0	32.0	41.0
30	36.94653618803433	39.0	35.0	41.0	32.0	41.0
31	36.8326999018937	39.0	35.0	40.0	31.0	41.0
32	36.64450481050644	39.0	35.0	40.0	31.0	41.0
33	36.36822015383728	39.0	35.0	40.0	30.0	41.0
34	36.145926939660505	39.0	35.0	40.0	30.0	41.0
35	35.98569214407611	39.0	35.0	40.0	29.0	41.0
36	35.78231118659159	39.0	35.0	40.0	27.0	41.0
37	35.648886617146346	39.0	35.0	40.0	27.0	41.0
38	35.50743217062252	38.0	35.0	40.0	26.0	41.0
39	35.4625876979645	38.0	35.0	40.0	26.0	41.0
40	35.242433860689054	38.0	34.0	40.0	25.0	41.0
41	35.17953040883122	38.0	34.0	40.0	24.0	41.0
42	35.07618490152271	38.0	34.0	40.0	24.0	41.0
43	35.00971994360949	38.0	34.0	40.0	24.0	41.0
44	34.859633791437545	38.0	34.0	40.0	23.0	41.0
45	34.76633387470424	38.0	34.0	40.0	23.0	41.0
46	34.60320123333636	38.0	34.0	40.0	23.0	41.0
47	34.479051419243675	38.0	33.0	40.0	23.0	41.0
48	34.35412252570138	38.0	33.0	40.0	22.0	41.0
49	34.177568282809965	37.0	33.0	40.0	22.0	41.0
50	34.001038772599486	37.0	33.0	40.0	20.0	41.0
51	31.868191299042845	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	17.0
10	13.0
11	14.0
12	10.0
13	4.0
14	14.0
15	13.0
16	31.0
17	64.0
18	128.0
19	220.0
20	390.0
21	598.0
22	935.0
23	1510.0
24	2268.0
25	3128.0
26	3757.0
27	4151.0
28	4254.0
29	4781.0
30	5682.0
31	7152.0
32	9655.0
33	13317.0
34	18084.0
35	23255.0
36	27820.0
37	42850.0
38	42610.0
39	25855.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.609825469714835	19.41762780612876	22.754890887655918	17.21765583650049
2	32.63848240269751	27.757075607805636	24.14734082458758	15.457101164909274
3	29.254639438733026	26.495708879856885	28.785955134916776	15.463696546493319
4	26.230656982448043	23.895891901695837	32.638894614046514	17.23455650180961
5	23.703389201711502	34.018978210508095	25.077289627938036	17.20034295984237
6	23.654748262529164	34.92254548752236	27.438848446375424	13.983857803573049
7	44.15401864844143	43.02785724296561	9.7549815741527	3.0631425344402583
8	74.56078880763745	7.925587607278003	13.382441445377873	4.13118213970667
9	69.13155312992077	7.69887136532643	14.373397528380751	8.796177976372045
10	38.738798156590846	24.850985597335466	21.755690577672983	14.654525668400703
11	30.339579709308556	25.13376258275143	26.84773737190532	17.678920336034693
12	29.038228480506522	21.280410892272688	30.826401312480932	18.85495931473985
13	22.458098716373858	24.99278630139245	33.00370165791405	19.545413324319647
14	18.572594540672892	29.175494859724477	30.48138041336554	21.77053018623709
15	17.519394543970584	26.11400117068023	38.5739136169897	17.79269066835948
16	20.21319570970428	24.359629669324057	34.386258522469646	21.040916098502024
17	19.739564869699993	23.71328227408757	32.37136944854366	24.17578340766878
18	20.862428584383785	25.818033422096175	31.94349406827869	21.37604392524135
19	23.557878595513493	26.37575537729705	28.657757405376884	21.408608621812576
20	25.70014097628136	24.719902388352555	30.879576576502306	18.70038005886378
21	23.588794446688706	26.716241951573412	30.203962175486616	19.491001426251266
22	22.53889214077842	24.36292736011608	28.48009431395665	24.61808618514885
23	22.47417495898497	27.65402277055492	29.918711921976637	19.953090348483475
24	22.91235562297501	23.758625522477885	30.930278572429653	22.398740282117448
25	21.442822163779812	27.926082260896806	28.740199675177458	21.890895900145924
26	20.511636726382353	27.234803828619008	29.81978119821595	22.43377824678269
27	22.318359069061888	28.11652390413613	28.34901110497374	21.21610592182824
28	19.879716728360965	27.468115452154628	31.847861035310025	20.804306784174383
29	21.894193590937945	27.266131891143225	30.34246518875158	20.49720932916725
30	20.134463342044732	27.646190754923865	31.404321623782945	20.815024279248455
31	22.110192337815445	26.725310601251472	28.154447348244393	23.01004971268869
32	25.814735731304157	28.023776350610486	27.504802262215883	18.656685655869477
33	22.444907953205767	26.94130934812897	29.148701122039295	21.46508157662597
34	22.249519773778413	27.568695021311328	30.64667716431569	19.535108040594572
35	23.304780827225734	26.267343792509294	28.593040223583436	21.834835156681535
36	23.247483449714338	28.445880771989412	27.86589940394239	20.44073637435386
37	23.657633741972184	28.11528727008912	29.474760299100556	18.75231868883814
38	22.634525173747083	26.578975572355457	28.259973453589126	22.526525800308335
39	24.9424965168141	24.170012448782742	30.206023232231633	20.68146780217153
40	24.163829278547695	25.40829534118733	31.98677625992399	18.441099120340983
41	21.077190697214277	27.78510597953783	30.490449063043606	20.647254260204292
42	23.226872882264193	26.598761717107593	29.38613485906494	20.788230541563273
43	24.26811874984542	26.64369275414891	27.41370355408625	21.67448494191942
44	22.372358755781264	26.75622645242669	28.311087660865482	22.56032713092657
45	20.98856525717866	26.396778156096197	28.19278300370166	24.421873583023487
46	22.806417306281276	28.48215537070167	27.39309298663611	21.318334336380946
47	21.69179781857754	26.702638977056314	31.51149657452369	20.09406662984245
48	22.687700437768452	25.52330230755913	28.572841867482296	23.21615538719012
49	20.229684163664395	26.294961952892486	30.834645539460993	22.640708343982126
50	20.014922050833903	25.94829220838108	30.45252561893534	23.584260121849677
51	20.75195594285102	26.29083983940246	27.372070207836963	25.58513400990956
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	192.0
1	509.5
2	827.0
3	3081.5
4	5336.0
5	3688.5
6	2041.0
7	1832.5
8	1624.0
9	1542.0
10	1460.0
11	1395.5
12	1331.0
13	1255.5
14	1180.0
15	1159.5
16	1139.0
17	1120.5
18	1102.0
19	1130.0
20	1158.0
21	1343.5
22	1529.0
23	1608.5
24	1688.0
25	1992.0
26	2602.5
27	2909.0
28	3403.5
29	3898.0
30	4398.0
31	4898.0
32	5347.5
33	5797.0
34	6294.0
35	6791.0
36	7520.5
37	8250.0
38	8804.5
39	9359.0
40	10339.0
41	11319.0
42	12442.5
43	13566.0
44	15800.5
45	18035.0
46	21304.0
47	24573.0
48	20848.5
49	17124.0
50	16191.0
51	15258.0
52	13636.5
53	12015.0
54	11341.5
55	10668.0
56	10565.0
57	10462.0
58	10775.5
59	11089.0
60	10839.5
61	10590.0
62	9118.0
63	7646.0
64	6260.5
65	4875.0
66	4084.5
67	3294.0
68	2841.0
69	2388.0
70	2104.0
71	1820.0
72	1537.5
73	1255.0
74	1003.0
75	605.0
76	459.0
77	367.5
78	276.0
79	209.5
80	143.0
81	112.5
82	82.0
83	61.5
84	41.0
85	31.5
86	22.0
87	25.0
88	28.0
89	17.5
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	242594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.54057396308235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.45116879683576	29.372119673198842
2	8.308167851223704	6.7363578654047505
3	4.230851355885672	5.14563426960271
4	2.938514880680027	4.76516319447307
5	2.1677902164739855	4.394172980370495
6	1.7417563981331787	4.236708245051403
7	1.3960487651119993	3.9617632752664944
8	1.1815066752076786	3.831916700330593
9	0.9344273963131298	3.409400067602661
>10	4.577575776062797	26.775600385829822
>50	0.03152040183428403	0.8800712301211078
>100	0.03253718899022868	2.5585958432607567
>500	0.0010167871559446462	0.275357181133911
>1k	0.007117510091612523	3.6571390883533805
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1862	0.7675375318433267	No Hit
GAATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC	1518	0.6257368277863427	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG	1406	0.5795691566980222	No Hit
GAATCTATCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC	1061	0.43735624129203526	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1012	0.4171578851908951	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGC	1009	0.4159212511438865	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG	1004	0.41386019439887217	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGC	668	0.275357181133911	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	415	0.17106770983618722	No Hit
GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	385	0.15870136936610138	No Hit
GAACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT	344	0.14180070405698408	No Hit
GCTGTCACTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	299	0.12325119335185536	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCT	291	0.11995350255983248	No Hit
GAATATGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC	277	0.11418254367379242	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTT	264	0.10882379613675523	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG	251	0.10346504859971804	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1221134900286074E-4	0.0	0.0	0.08079342440456071	0.0
2	4.1221134900286074E-4	0.0	0.0	0.4526080612051411	0.0
3	4.1221134900286074E-4	0.0	0.0	0.579981368047025	0.0
4	4.1221134900286074E-4	0.0	0.0	0.8479187448988845	0.0
5	4.1221134900286074E-4	0.0	0.0	1.8780349060570336	0.0
6	4.1221134900286074E-4	0.0	0.0	2.2622158833276997	0.0
7	4.1221134900286074E-4	0.0	0.0	2.61960312291318	0.0
8	4.1221134900286074E-4	0.0	0.0	3.276255801874737	0.0
9	4.1221134900286074E-4	0.0	0.0	3.4918423374032335	0.0
10	4.1221134900286074E-4	0.0	0.0	5.109771882239462	0.0
11	4.1221134900286074E-4	0.0	0.0	5.689341038937484	0.0
12	4.1221134900286074E-4	0.0	0.0	6.959364205215298	0.0
13	4.1221134900286074E-4	0.0	0.0	7.256156376497358	0.0
14	4.1221134900286074E-4	0.0	0.0	7.392186121668302	0.0
15	4.1221134900286074E-4	0.0	0.0	7.631680915438964	0.0
16	4.1221134900286074E-4	0.0	0.0	7.947434808775156	0.0
17	4.1221134900286074E-4	0.0	0.0	8.28256263551448	0.0
18	4.1221134900286074E-4	0.0	0.0	8.667568035483153	0.0
19	4.1221134900286074E-4	0.0	0.0	8.895933122830737	0.0
20	4.1221134900286074E-4	0.0	0.0	9.100389951936156	0.0
21	4.1221134900286074E-4	0.0	0.0	9.367915117439013	0.0
22	4.1221134900286074E-4	0.0	0.0	9.650279891505972	0.0
23	4.1221134900286074E-4	0.0	0.0	9.945835428741024	0.0
24	8.244226980057215E-4	0.0	0.0	10.170490613947583	0.0
25	8.244226980057215E-4	0.0	0.0	10.384428304080068	0.0
26	8.244226980057215E-4	0.0	0.0	10.598365994212553	0.0
27	8.244226980057215E-4	0.0	0.0	10.800761766572958	0.0
28	8.244226980057215E-4	0.0	0.0	11.005218595678377	0.0
29	8.244226980057215E-4	0.0	0.0	11.226576090092912	0.0
30	8.244226980057215E-4	0.0	0.0	11.444635893715425	0.0
31	8.244226980057215E-4	0.0	0.0	11.706802311681246	0.0
32	8.244226980057215E-4	0.0	0.0	11.947945950847918	0.0
33	8.244226980057215E-4	0.0	0.0	12.174662192799492	0.0
34	8.244226980057215E-4	0.0	0.0	12.385714403488956	0.0
35	8.244226980057215E-4	0.0	0.0	12.617377181628564	0.0
36	8.244226980057215E-4	0.0	0.0	12.84574226897615	0.0
37	8.244226980057215E-4	0.0	0.0	13.09224465567986	0.0
38	8.244226980057215E-4	0.0	0.0	13.338334831034569	0.0
39	8.244226980057215E-4	0.0	0.0	13.572470877268193	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	30	2.1583983E-6	45.000004	26
GGCCGAT	60	0.0	45.000004	8
CGACCAA	30	2.1583983E-6	45.000004	29
CACGACC	30	2.1583983E-6	45.000004	27
AGGCGAT	130	0.0	45.000004	7
AACGGGC	30	2.1583983E-6	45.000004	4
CACGTGA	30	2.1583983E-6	45.000004	43
TAATACG	35	1.2066266E-7	45.0	4
TCGCAAG	20	7.022272E-4	45.0	2
CGACGGT	20	7.022272E-4	45.0	28
AGGACTA	35	1.2066266E-7	45.0	7
ATAGCGG	25	3.881679E-5	45.0	2
GCGTTAA	20	7.022272E-4	45.0	1
ACGGGCC	25	3.881679E-5	45.0	5
CGTTAGG	20	7.022272E-4	45.0	2
GCCGATA	20	7.022272E-4	45.0	9
GTTCGCC	20	7.022272E-4	45.0	41
CGGATAC	20	7.022272E-4	45.0	16
TCGTAAG	20	7.022272E-4	45.0	2
TAGGCGA	25	3.881679E-5	45.0	6
>>END_MODULE