##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936134.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	419820
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.846648563670144	31.0	31.0	34.0	30.0	34.0
2	31.989352579677004	33.0	31.0	34.0	30.0	34.0
3	31.946605688152065	33.0	31.0	34.0	30.0	34.0
4	35.66933209470726	37.0	35.0	37.0	33.0	37.0
5	31.154218474584347	37.0	35.0	37.0	0.0	37.0
6	33.3685484255157	37.0	35.0	37.0	17.0	37.0
7	29.563622504883046	35.0	32.0	37.0	0.0	37.0
8	32.604944976418466	35.0	33.0	37.0	17.0	37.0
9	36.41123338573674	39.0	35.0	39.0	32.0	39.0
10	36.736839597922916	38.0	35.0	39.0	33.0	39.0
11	36.87081606402744	39.0	37.0	39.0	33.0	39.0
12	36.867490829403074	39.0	35.0	39.0	33.0	39.0
13	36.66306750512124	39.0	35.0	39.0	33.0	39.0
14	37.88318565099328	40.0	37.0	41.0	33.0	41.0
15	37.98750416845315	40.0	37.0	41.0	33.0	41.0
16	38.04777761897956	40.0	37.0	41.0	33.0	41.0
17	38.00979467390786	40.0	37.0	41.0	33.0	41.0
18	37.927864322805014	40.0	36.0	41.0	33.0	41.0
19	37.85024534324234	40.0	36.0	41.0	33.0	41.0
20	37.79746319851365	40.0	36.0	41.0	33.0	41.0
21	37.69676051641179	40.0	36.0	41.0	33.0	41.0
22	37.69444523843552	39.0	36.0	41.0	33.0	41.0
23	37.63638225906341	39.0	36.0	41.0	33.0	41.0
24	37.586165499499785	39.0	36.0	41.0	33.0	41.0
25	37.444738221142394	39.0	35.0	41.0	32.0	41.0
26	37.29120099090086	39.0	35.0	41.0	32.0	41.0
27	37.23425277499881	39.0	35.0	41.0	32.0	41.0
28	37.143566290314894	39.0	35.0	41.0	32.0	41.0
29	37.11317469391644	39.0	35.0	41.0	32.0	41.0
30	36.975351341050924	39.0	35.0	41.0	31.0	41.0
31	36.86035681958935	39.0	35.0	41.0	31.0	41.0
32	36.775251298175405	39.0	35.0	41.0	31.0	41.0
33	36.613741603544376	39.0	35.0	41.0	30.0	41.0
34	36.4955171263875	39.0	35.0	40.0	30.0	41.0
35	36.41737173074175	39.0	35.0	40.0	30.0	41.0
36	36.293311419179645	39.0	35.0	40.0	30.0	41.0
37	36.224815397074934	39.0	35.0	40.0	30.0	41.0
38	36.12335524748702	39.0	35.0	40.0	29.0	41.0
39	36.12162117097804	39.0	35.0	40.0	29.0	41.0
40	35.97186175027392	39.0	35.0	40.0	29.0	41.0
41	35.88440998523176	38.0	35.0	40.0	28.0	41.0
42	35.86553522938402	38.0	35.0	40.0	28.0	41.0
43	35.69218950978991	38.0	35.0	40.0	28.0	41.0
44	35.50782716402268	38.0	34.0	40.0	27.0	41.0
45	35.37301700728884	38.0	34.0	40.0	26.0	41.0
46	35.25815587632795	38.0	34.0	40.0	26.0	41.0
47	35.11664522890763	38.0	34.0	40.0	26.0	41.0
48	35.012009909008626	38.0	34.0	40.0	26.0	41.0
49	34.85018102996522	38.0	34.0	40.0	24.0	41.0
50	34.66849364013149	37.0	34.0	40.0	24.0	41.0
51	32.59721309132485	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	25.0
10	19.0
11	16.0
12	13.0
13	18.0
14	8.0
15	26.0
16	69.0
17	113.0
18	204.0
19	378.0
20	596.0
21	943.0
22	1397.0
23	2022.0
24	2724.0
25	3470.0
26	4386.0
27	5141.0
28	6193.0
29	7490.0
30	9319.0
31	11756.0
32	15474.0
33	21619.0
34	30842.0
35	37016.0
36	42188.0
37	64206.0
38	78683.0
39	73448.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.765709113429565	21.050926587585156	24.694392835024534	11.488971463960745
2	32.23881663570101	24.18393597255967	25.480920394454763	18.09632699728455
3	33.1544471440141	24.68486494211805	25.456862464865893	16.703825449001954
4	29.63508170168167	25.797961030918014	25.06240769853747	19.504549568862846
5	22.273355247487018	40.226287456528986	20.5781049021009	16.92225239388309
6	25.66695250345386	36.52017531322948	24.125577628507454	13.687294554809204
7	68.958124910676	20.142680196274593	6.770997093992663	4.128197799056739
8	82.45462341003287	6.146205516649992	7.391024724882092	4.0081463484350435
9	76.20289647944357	8.449573626792436	9.721070935162688	5.626458958601305
10	40.94564337096851	28.205659568386448	17.1804582916488	13.668238768996236
11	31.49159163451003	27.25287027773808	23.35453289505026	17.901005192701632
12	29.576008765661477	23.636082130436854	27.824067457481778	18.963841646419894
13	24.53003668238769	26.30865609070554	28.67300271544948	20.488304511457294
14	19.844457148301654	29.921871278166833	28.82949835643847	21.40417321709304
15	19.79848506502787	28.562002763088945	32.40364918298319	19.235862988900003
16	22.498213520080036	27.001810299652234	30.26320804154161	20.23676813872612
17	22.01848411223858	26.652851221952268	27.987709018150635	23.34095564765852
18	22.759039588395026	27.349101996093566	29.411176218379303	20.480682197132104
19	24.774665332761657	28.169453575341812	26.5344671525892	20.521413939307322
20	26.19122481063313	28.374303272831213	26.463484350435902	18.970987566099755
21	24.65413748749464	28.612262398170646	27.345529036253634	19.388071078081083
22	22.901481587346957	26.78219236815778	26.776237435091225	23.54008860940403
23	22.88790433995522	27.618026773379068	28.160640274403313	21.3334286122624
24	22.806679052927446	26.029488828545567	29.70892287170692	21.454909246820066
25	21.67047782382926	28.155161735982087	27.406031156209803	22.768329283978847
26	21.38726120718403	29.153208517936257	27.13948835215092	22.32004192272879
27	21.948454099375923	29.049592682578247	27.89338287837645	21.108570339669384
28	19.961650231051404	28.150874184174167	29.92330046210281	21.964175122671623
29	21.65999714163213	27.15949692725454	29.167738554618644	22.012767376494686
30	22.011576389881377	28.026535181744556	27.87694726311276	22.084941165261302
31	23.31141917964842	28.357153065599544	26.542327664237053	21.78910009051498
32	23.856176456576627	27.556571864132245	27.19189176313658	21.395359916154543
33	22.36577580867991	28.542470582630653	26.590443523414798	22.501310085274643
34	21.156686198847126	28.222809775618124	27.97794292792149	22.64256109761326
35	21.713829736553762	27.342670668381686	27.780715544757278	23.162784050307277
36	22.008718022009433	30.05430898956696	26.312705445190797	21.624267543232815
37	20.711971797436995	29.462626840074318	29.020770806536135	20.804630555952553
38	20.580725072650182	29.361869372588252	27.487018245914918	22.570387308846648
39	21.818160163879757	28.948120623124197	27.595159830403503	21.63855938259254
40	22.572292887427945	26.736220284884	28.6232194750131	22.068267352674955
41	20.29250631222905	27.56276499452146	27.378876661426325	24.76585203182316
42	20.8263065123148	27.344814444285646	27.527035396122145	24.301843647277405
43	21.841503501500643	26.40869896622362	28.544137963889288	23.20565956838645
44	20.99923776856748	26.93440045733886	28.030108141584485	24.036253632509172
45	20.928969558382164	27.757848601781713	27.31551617359821	23.99766566623791
46	21.37368395979229	28.97718069648897	26.455862036110716	23.193273307608024
47	20.224381877947692	27.751893668715166	29.06912486303654	22.954599590300607
48	20.26511362012291	26.268638940498306	29.97427468915249	23.491972750226285
49	20.57929588871421	26.709780382068505	29.559811347720448	23.151112381496834
50	19.053642037063504	27.044447620408747	29.595302748797103	24.306607593730646
51	19.747749035300842	26.215282740222	28.073460054308992	25.963508170168165
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	302.0
1	714.5
2	1127.0
3	1369.5
4	1612.0
5	1288.0
6	964.0
7	868.5
8	773.0
9	801.0
10	829.0
11	826.5
12	824.0
13	872.5
14	921.0
15	928.0
16	935.0
17	965.5
18	996.0
19	1173.0
20	1350.0
21	1694.0
22	2038.0
23	2312.5
24	2587.0
25	3248.0
26	4742.5
27	5576.0
28	6461.5
29	7347.0
30	8302.0
31	9257.0
32	10059.0
33	10861.0
34	11606.0
35	12351.0
36	13775.0
37	15199.0
38	17034.0
39	18869.0
40	21188.0
41	23507.0
42	26232.0
43	28957.0
44	32341.5
45	35726.0
46	36548.5
47	37371.0
48	35901.5
49	34432.0
50	31187.5
51	27943.0
52	25494.5
53	23046.0
54	21384.0
55	19722.0
56	19088.5
57	18455.0
58	17558.5
59	16662.0
60	16208.0
61	15754.0
62	14538.0
63	13322.0
64	11188.0
65	9054.0
66	7428.5
67	5803.0
68	4955.0
69	4107.0
70	3464.5
71	2822.0
72	2266.0
73	1710.0
74	1428.0
75	930.5
76	715.0
77	541.0
78	367.0
79	307.0
80	247.0
81	182.0
82	117.0
83	93.5
84	70.0
85	55.0
86	40.0
87	41.0
88	42.0
89	39.5
90	37.0
91	24.5
92	12.0
93	7.5
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	419820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.66249181948897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.66853972695533	28.82215362454575
2	9.628778362562329	7.638026860736015
3	4.336144595238894	5.159468986103518
4	2.62934338010703	4.171452412164902
5	1.8641599153622692	3.696861369663763
6	1.2659109208745973	3.012550892603428
7	0.9699703638231166	2.693000913419677
8	0.7527008285644554	2.388319236436822
9	0.6402534009796963	2.285464075088148
>10	5.16352542693339	36.94224987351002
>50	0.05916025763926411	1.5640584753026345
>100	0.02031766423974727	1.3602260326961386
>500	0.0011951567199851335	0.26616724772917993
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	570	0.13577247391739317	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	553	0.1317231194321376	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3819732266209328E-4	0.0	0.0	0.10909437377923872	0.0
2	2.3819732266209328E-4	0.0	0.0	0.518317374112715	0.0
3	2.3819732266209328E-4	0.0	0.0	0.7081606402744033	0.0
4	2.3819732266209328E-4	0.0	0.0	0.9523128960030489	0.0
5	2.3819732266209328E-4	0.0	0.0	1.7073984088418845	0.0
6	2.3819732266209328E-4	0.0	0.0	2.16545186032109	0.0
7	2.3819732266209328E-4	0.0	0.0	2.6151684055071223	0.0
8	2.3819732266209328E-4	0.0	0.0	3.4236101186222667	0.0
9	2.3819732266209328E-4	0.0	0.0	3.7111142870754135	0.0
10	2.3819732266209328E-4	0.0	0.0	4.522176170739841	0.0
11	2.3819732266209328E-4	0.0	0.0	5.560478300223905	0.0
12	2.3819732266209328E-4	0.0	0.0	6.371778381210995	0.0
13	2.3819732266209328E-4	0.0	0.0	6.716211709780382	0.0
14	2.3819732266209328E-4	0.0	0.0	6.845552855985899	0.0
15	2.3819732266209328E-4	0.0	0.0	7.114954027916726	0.0
16	2.3819732266209328E-4	0.0	0.0	7.739983802582059	0.0
17	2.3819732266209328E-4	0.0	0.0	8.431232432947454	0.0
18	2.3819732266209328E-4	0.0	0.0	9.183697775237006	0.0
19	2.3819732266209328E-4	0.0	0.0	9.671287694726312	0.0
20	2.3819732266209328E-4	0.0	0.0	10.110285360392549	0.0
21	2.3819732266209328E-4	0.0	0.0	10.67933876423229	0.0
22	2.3819732266209328E-4	0.0	0.0	11.29722261921776	0.0
23	2.3819732266209328E-4	0.0	0.0	11.860082892668286	0.0
24	2.3819732266209328E-4	0.0	0.0	12.348387404125578	0.0
25	2.3819732266209328E-4	0.0	0.0	12.766423705397552	0.0
26	2.3819732266209328E-4	0.0	0.0	13.15873469582202	0.0
27	4.7639464532418655E-4	0.0	0.0	13.534610070982803	0.0
28	4.7639464532418655E-4	0.0	0.0	13.950026201705493	0.0
29	4.7639464532418655E-4	0.0	0.0	14.376875803915963	0.0
30	4.7639464532418655E-4	0.0	0.0	14.844457148301654	0.0
31	4.7639464532418655E-4	0.0	0.0	15.267495593349532	0.0
32	4.7639464532418655E-4	0.0	0.0	15.708637034919727	0.0
33	4.7639464532418655E-4	0.0	0.0	16.13453384783955	0.0
34	4.7639464532418655E-4	0.0	0.0	16.582106617121624	0.0
35	4.7639464532418655E-4	0.0	0.0	16.996808155876327	0.0
36	4.7639464532418655E-4	0.0	0.0	17.43294745367062	0.0
37	4.7639464532418655E-4	0.0	0.0	17.857891477299795	0.0
38	4.7639464532418655E-4	0.0	0.0	18.305464246581867	0.0
39	4.7639464532418655E-4	0.0	0.0	18.75946834357582	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.028165E-4	45.0	1
CGAACAT	20	7.028165E-4	45.0	31
CGTATGG	25	3.886562E-5	45.0	2
CACGACC	20	7.028165E-4	45.0	27
CCTAACG	20	7.028165E-4	45.0	18
CGTTACC	20	7.028165E-4	45.0	24
ATCCGAG	20	7.028165E-4	45.0	17
CCCCGAT	20	7.028165E-4	45.0	40
GTAGACG	20	7.028165E-4	45.0	1
CTTGCGG	45	3.8380676E-10	45.0	2
TCGTAAG	20	7.028165E-4	45.0	1
TCATCGA	25	3.886562E-5	45.0	16
ATGCGAT	25	3.886562E-5	45.0	18
AGCCTAA	20	7.028165E-4	45.0	16
TCAAGCG	25	3.886562E-5	45.0	17
AATACGG	20	7.028165E-4	45.0	2
GCCGTCA	20	7.028165E-4	45.0	29
AGTAATC	20	7.028165E-4	45.0	31
TAGTACG	25	3.886562E-5	45.0	1
AACACGA	25	3.886562E-5	45.0	37
>>END_MODULE