FastQCFastQC Report
Sat 14 Jan 2017
SRR2936133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767341
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117661.5333469735098215No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35540.4631578398651968No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23270.30325500657465193No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16350.21307345756319548No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC9640.1256286318598902No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG253.8895887E-545.01
CGGTCTA207.03182E-445.031
CGTTATT9650.043.3678741
CGGTTTT13200.043.2954521
CGTTTTT47800.042.834731
ATTACGG556.002665E-1140.9090922
CGTTTTA5750.040.6956521
TTACGGG2200.039.8863643
ATGGGCG1700.039.7058835
AGTACGG1200.039.3749962
TACGGGA2250.039.04
CGTTCGA356.2455747E-638.57142614
CTTGCGG700.038.5714262
ACGGGAT3200.037.968755
TCGTACG301.1395682E-437.49999630
CTTACGG552.746674E-936.818182
GTACGGG3450.036.521743
TACGGGT751.8189894E-1236.04
TATGCGT250.002106672936.015
GCGATAT504.8752554E-836.09
Produced by FastQC (version 0.11.5)