##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936133.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767341
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.749151941574866	31.0	31.0	34.0	30.0	34.0
2	31.837426906681646	33.0	31.0	34.0	30.0	34.0
3	31.2188635821623	31.0	31.0	34.0	28.0	34.0
4	35.27511367175741	37.0	35.0	37.0	33.0	37.0
5	30.998492195777366	37.0	35.0	37.0	0.0	37.0
6	33.29834454303889	37.0	35.0	37.0	17.0	37.0
7	29.67130780187687	35.0	32.0	37.0	0.0	37.0
8	32.707677551440625	35.0	35.0	37.0	17.0	37.0
9	36.57438218471319	39.0	35.0	39.0	32.0	39.0
10	36.92005640256418	39.0	37.0	39.0	34.0	39.0
11	37.04348132056022	39.0	37.0	39.0	34.0	39.0
12	37.06920391325369	39.0	37.0	39.0	34.0	39.0
13	36.93566745423482	39.0	37.0	39.0	33.0	39.0
14	38.16719033649968	40.0	38.0	41.0	33.0	41.0
15	38.23941637420651	40.0	38.0	41.0	33.0	41.0
16	38.2745545982816	40.0	37.0	41.0	33.0	41.0
17	38.230632795588924	40.0	37.0	41.0	33.0	41.0
18	38.05167063925947	40.0	37.0	41.0	33.0	41.0
19	37.87117982748217	40.0	37.0	41.0	33.0	41.0
20	37.63678468894533	39.0	35.0	41.0	33.0	41.0
21	37.52910114277746	39.0	35.0	41.0	33.0	41.0
22	37.49063845148376	39.0	35.0	41.0	33.0	41.0
23	37.438021687880614	39.0	35.0	41.0	33.0	41.0
24	37.36584256543049	39.0	35.0	41.0	33.0	41.0
25	37.23703281852527	39.0	35.0	41.0	33.0	41.0
26	37.06831512977933	39.0	35.0	41.0	32.0	41.0
27	37.02055148884264	39.0	35.0	41.0	32.0	41.0
28	36.90370382919719	39.0	35.0	41.0	32.0	41.0
29	36.83139829619426	39.0	35.0	41.0	32.0	41.0
30	36.64803913774971	39.0	35.0	40.0	31.0	41.0
31	36.43442745793591	39.0	35.0	40.0	30.0	41.0
32	36.20527900894127	38.0	35.0	40.0	30.0	41.0
33	35.8930201826828	38.0	35.0	40.0	29.0	41.0
34	35.50293937115311	38.0	35.0	40.0	25.0	41.0
35	35.17421850259532	38.0	34.0	40.0	24.0	41.0
36	34.92451074554859	38.0	34.0	40.0	23.0	41.0
37	34.708819156020596	38.0	34.0	40.0	22.0	41.0
38	34.52608944393692	38.0	34.0	40.0	21.0	41.0
39	34.41876558140383	38.0	34.0	40.0	20.0	41.0
40	34.27599854562704	38.0	33.0	40.0	19.0	41.0
41	34.12689664699267	38.0	33.0	40.0	18.0	41.0
42	34.05823747199746	38.0	33.0	40.0	18.0	41.0
43	33.88088346641194	38.0	33.0	40.0	18.0	41.0
44	33.70133356617202	38.0	33.0	40.0	16.0	41.0
45	33.58046292326358	37.0	33.0	40.0	17.0	41.0
46	33.49761839912112	37.0	33.0	40.0	15.0	41.0
47	33.33293542245234	37.0	32.0	40.0	15.0	41.0
48	33.21804386837143	37.0	32.0	40.0	15.0	41.0
49	33.05872226298347	37.0	32.0	40.0	15.0	41.0
50	32.89224608094706	36.0	31.0	40.0	14.0	41.0
51	31.023280392941338	35.0	28.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	23.0
9	26.0
10	37.0
11	33.0
12	20.0
13	35.0
14	39.0
15	67.0
16	128.0
17	206.0
18	424.0
19	817.0
20	1405.0
21	2387.0
22	3756.0
23	5805.0
24	9248.0
25	13634.0
26	17191.0
27	17925.0
28	17583.0
29	17694.0
30	18988.0
31	22589.0
32	27635.0
33	38092.0
34	52796.0
35	61962.0
36	70620.0
37	108573.0
38	132460.0
39	125128.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.91581448143654	19.008628497630127	22.30207951875372	19.773477502179603
2	38.74535571538599	22.66658499936795	23.1863017876016	15.401757497644464
3	31.13948036140386	22.575882169726366	31.324665305255422	14.959972163614351
4	25.602176867911396	23.958839681445408	33.50413961980397	16.93484383083922
5	20.109964148924664	37.19206975777392	27.130962635907636	15.56700345739378
6	23.575046817516593	32.21996478749343	31.15238205699943	13.052606337990541
7	64.36225876109839	18.46349927867793	13.687786785796666	3.4864551744270145
8	78.29296232053285	4.117465377192148	14.78234578889959	2.8072265133754093
9	73.15430818892774	6.062754368657481	16.04488747505998	4.738049967354801
10	37.48489915174609	26.226019462012324	23.48538654913526	12.803694837106319
11	27.524529511651274	23.874652859680374	31.679787734527416	16.921029894140936
12	24.781420515781118	21.96767799452916	34.90104138837883	18.34986010131089
13	23.014930780448328	21.632885509832004	36.69424675600548	18.65793695371419
14	19.937811220826205	24.267828774951422	36.24803053661931	19.54632946760306
15	19.996455291715158	24.086683755983323	37.84953495251785	18.06732599978367
16	24.227559846274342	23.810014061544997	34.6138157611805	17.34861033100017
17	23.78968411697016	23.44407505919793	33.95465640438866	18.811584419443246
18	23.65258731124754	23.690249836774	34.44257507418475	18.214587777793707
19	22.57640345035649	26.052823972653616	32.819828472608656	18.550944104381234
20	23.76375040562149	26.05034788966053	32.99224204102218	17.1936596636958
21	23.28560574764023	25.081026557944902	34.60391142920813	17.029456265206733
22	22.341305886170556	22.722883307421345	35.04830316638887	19.887507640019237
23	21.52081017435534	23.876086381413216	35.171064754783075	19.432038689448365
24	21.590140498161833	24.249844593212146	34.751564167690766	19.40845074093526
25	22.452729620859564	24.909394910476568	32.807448057643214	19.830427411020654
26	20.838193189207928	25.284195683535742	33.07551662168449	20.802094505571837
27	20.604659466912363	24.329079248990997	34.39644173841877	20.669819545677868
28	19.35580139729273	25.39236141428648	34.21777280244376	21.03406438597703
29	20.903092627658367	24.951879281831676	34.08771328522782	20.057314805282136
30	21.739096438219775	23.692074318979436	34.908469637358095	19.66035960544269
31	21.897565749777478	23.97760578412987	34.45313100694476	19.67169745914789
32	22.559722470192522	24.4652638136109	33.49866617318767	19.47634754300891
33	22.54486597223399	24.64028378517504	32.65588571443465	20.15896452815632
34	21.151352527754934	24.686026160468423	34.89348281924203	19.26913849253461
35	20.36330653516494	25.691837136292726	34.34496527619403	19.599891052348305
36	21.854299457477183	26.802164878456907	32.22335311158924	19.12018255247667
37	21.35387005255812	27.088217624237465	31.918534263124215	19.639378060080197
38	22.157163503579245	26.569022116633935	31.323883384310236	19.949930995476585
39	22.332313795300916	25.99496182270985	30.776408402522478	20.896315979466756
40	23.042949614317493	24.55218735868408	32.37022392912669	20.034639097871743
41	21.121378891522806	25.267514703371774	31.88816966641949	21.72293673868593
42	21.389838416036678	25.859428858877603	31.83877832671524	20.911954398370476
43	21.6106007628942	25.304004347480458	31.320625380371958	21.76476950925338
44	21.887009817017468	25.197923739250218	31.048256251132155	21.866810192600163
45	21.344096040743292	25.141104150566697	30.672673557127794	22.842126251562213
46	21.96898119610447	25.4508751650179	30.589008015992892	21.99113562288474
47	20.488804846867296	25.694573859600883	32.538336932341686	21.278284361190135
48	20.81278075848938	25.37972035900597	32.14059981155705	21.666899070947597
49	21.836315275737906	24.86573765770368	31.95645742896574	21.341489637592673
50	20.68402444284875	25.1503568817514	32.30089360532019	21.864725070079665
51	20.269085061270022	24.803314302246328	31.98760394661565	22.939996689867996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	464.0
1	896.0
2	1328.0
3	12850.5
4	24373.0
5	18469.0
6	12565.0
7	9953.0
8	7341.0
9	6931.5
10	6522.0
11	6345.0
12	6168.0
13	5924.0
14	5680.0
15	5435.5
16	5191.0
17	4915.0
18	4639.0
19	4470.0
20	4301.0
21	4289.5
22	4278.0
23	4716.5
24	5155.0
25	5652.0
26	7107.5
27	8066.0
28	9057.0
29	10048.0
30	11629.5
31	13211.0
32	15256.5
33	17302.0
34	19332.0
35	21362.0
36	23517.5
37	25673.0
38	28031.5
39	30390.0
40	32830.5
41	35271.0
42	39416.5
43	43562.0
44	49103.0
45	54644.0
46	55883.0
47	57122.0
48	56924.5
49	56727.0
50	52982.5
51	49238.0
52	46176.0
53	43114.0
54	41084.5
55	39055.0
56	37212.5
57	35370.0
58	33569.0
59	31768.0
60	30051.0
61	28334.0
62	25211.0
63	22088.0
64	19277.0
65	16466.0
66	13920.0
67	11374.0
68	9810.0
69	8246.0
70	6788.5
71	5331.0
72	4478.5
73	3626.0
74	2998.0
75	1910.5
76	1451.0
77	1167.5
78	884.0
79	687.0
80	490.0
81	378.5
82	267.0
83	211.5
84	156.0
85	118.5
86	81.0
87	64.0
88	47.0
89	30.0
90	13.0
91	14.5
92	16.0
93	16.5
94	17.0
95	10.0
96	3.0
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	767341.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.23874310381757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.87207711130849	33.418799189838865
2	9.585307325242088	8.67254511315535
3	4.436441306947579	6.0209708574049845
4	2.7210737547183266	4.923918262249708
5	1.8358189485249958	4.152507089872139
6	1.3408005469704498	3.6393678898712563
7	1.0195948760138975	3.2287633466173014
8	0.8095651483714106	2.9298967818382553
9	0.638184009095199	2.5983578196379873
>10	3.7037327668682494	25.722553480414483
>50	0.021332111284776713	0.6582083839019488
>100	0.01344211552932588	1.073039892541025
>500	0.0014610995140233662	0.4120568368860742
>1k	8.766597084140197E-4	0.9935897292382848
>5k	0.0	0.0
>10k+	2.922199028046732E-4	1.555425326532419
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11766	1.5333469735098215	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3554	0.4631578398651968	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2327	0.30325500657465193	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1635	0.21307345756319548	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC	964	0.1256286318598902	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06242335545735208	0.0
2	0.0	0.0	0.0	0.2169830622891257	0.0
3	0.0	0.0	0.0	0.3990403223599417	0.0
4	0.0	0.0	0.0	0.5739299737665523	0.0
5	0.0	0.0	0.0	1.1874772754225305	0.0
6	0.0	0.0	0.0	1.8085831462153072	0.0
7	0.0	0.0	0.0	2.253365843868632	0.0
8	0.0	0.0	0.0	2.9084852757770014	0.0
9	0.0	0.0	0.0	3.3403662778347565	0.0
10	0.0	0.0	0.0	4.039794563303668	0.0
11	1.3032015753100643E-4	0.0	0.0	4.659075951891011	0.0
12	1.3032015753100643E-4	0.0	0.0	5.219452629274338	0.0
13	1.3032015753100643E-4	0.0	0.0	5.507590497575394	1.3032015753100643E-4
14	1.3032015753100643E-4	0.0	0.0	5.706328737810178	1.3032015753100643E-4
15	1.3032015753100643E-4	0.0	0.0	5.879915187641479	1.3032015753100643E-4
16	1.3032015753100643E-4	0.0	0.0	6.1438135066417665	1.3032015753100643E-4
17	1.3032015753100643E-4	0.0	0.0	6.452802600147783	1.3032015753100643E-4
18	1.3032015753100643E-4	0.0	0.0	6.809228230995085	2.6064031506201285E-4
19	1.3032015753100643E-4	0.0	0.0	7.038722028407188	2.6064031506201285E-4
20	1.3032015753100643E-4	0.0	0.0	7.277989837634116	2.6064031506201285E-4
21	1.3032015753100643E-4	0.0	0.0	7.542409437264528	2.6064031506201285E-4
22	1.3032015753100643E-4	0.0	0.0	7.842927720531028	2.6064031506201285E-4
23	1.3032015753100643E-4	0.0	0.0	8.145009845687902	2.6064031506201285E-4
24	1.3032015753100643E-4	0.0	0.0	8.417378974927704	2.6064031506201285E-4
25	1.3032015753100643E-4	0.0	0.0	8.643484448244001	2.6064031506201285E-4
26	1.3032015753100643E-4	0.0	0.0	8.880667134950432	2.6064031506201285E-4
27	1.3032015753100643E-4	0.0	0.0	9.146129295841092	2.6064031506201285E-4
28	1.3032015753100643E-4	0.0	0.0	9.39842912082112	2.6064031506201285E-4
29	1.3032015753100643E-4	0.0	0.0	9.66754024612265	2.6064031506201285E-4
30	1.3032015753100643E-4	0.0	0.0	9.985912390970897	2.6064031506201285E-4
31	1.3032015753100643E-4	0.0	0.0	10.254762875957365	2.6064031506201285E-4
32	1.3032015753100643E-4	0.0	0.0	10.527262325354698	2.6064031506201285E-4
33	1.3032015753100643E-4	0.0	0.0	10.798719213491784	2.6064031506201285E-4
34	1.3032015753100643E-4	0.0	0.0	11.08412035848469	2.6064031506201285E-4
35	1.3032015753100643E-4	0.0	0.0	11.386463123956624	2.6064031506201285E-4
36	1.3032015753100643E-4	0.0	0.0	11.696885739195482	2.6064031506201285E-4
37	1.3032015753100643E-4	0.0	0.0	12.007308354434338	2.6064031506201285E-4
38	1.3032015753100643E-4	0.0	0.0	12.362952064336456	2.6064031506201285E-4
39	1.3032015753100643E-4	0.0	0.0	12.827934386407087	2.6064031506201285E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTGCG	25	3.8895887E-5	45.0	1
CGGTCTA	20	7.03182E-4	45.0	31
CGTTATT	965	0.0	43.367874	1
CGGTTTT	1320	0.0	43.295452	1
CGTTTTT	4780	0.0	42.83473	1
ATTACGG	55	6.002665E-11	40.909092	2
CGTTTTA	575	0.0	40.695652	1
TTACGGG	220	0.0	39.886364	3
ATGGGCG	170	0.0	39.705883	5
AGTACGG	120	0.0	39.374996	2
TACGGGA	225	0.0	39.0	4
CGTTCGA	35	6.2455747E-6	38.571426	14
CTTGCGG	70	0.0	38.571426	2
ACGGGAT	320	0.0	37.96875	5
TCGTACG	30	1.1395682E-4	37.499996	30
CTTACGG	55	2.746674E-9	36.81818	2
GTACGGG	345	0.0	36.52174	3
TACGGGT	75	1.8189894E-12	36.0	4
TATGCGT	25	0.0021066729	36.0	15
GCGATAT	50	4.8752554E-8	36.0	9
>>END_MODULE