##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936132.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 587554 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.937212579609703 33.0 31.0 34.0 30.0 34.0 2 32.07144534800206 33.0 31.0 34.0 30.0 34.0 3 31.97604305306406 33.0 31.0 34.0 30.0 34.0 4 35.7071826589556 37.0 35.0 37.0 35.0 37.0 5 31.116756927873862 37.0 35.0 37.0 0.0 37.0 6 33.380060385939 37.0 35.0 37.0 17.0 37.0 7 29.661510261184503 35.0 32.0 37.0 0.0 37.0 8 32.69533183332936 35.0 35.0 37.0 17.0 37.0 9 36.553525292994344 39.0 35.0 39.0 32.0 39.0 10 36.85181957743458 38.0 37.0 39.0 33.0 39.0 11 36.982840726129005 39.0 37.0 39.0 33.0 39.0 12 37.04711737133948 39.0 37.0 39.0 33.0 39.0 13 36.901117173910826 39.0 37.0 39.0 33.0 39.0 14 38.17842785514183 40.0 37.0 41.0 33.0 41.0 15 38.22454446740215 40.0 37.0 41.0 33.0 41.0 16 38.27219285376323 40.0 37.0 41.0 34.0 41.0 17 38.240767657100456 40.0 37.0 41.0 34.0 41.0 18 38.13962291125581 40.0 37.0 41.0 33.0 41.0 19 38.05386568723896 40.0 37.0 41.0 34.0 41.0 20 38.0097744207341 40.0 37.0 41.0 33.0 41.0 21 37.907031523910995 40.0 37.0 41.0 33.0 41.0 22 37.89956838009783 40.0 37.0 41.0 33.0 41.0 23 37.84741487590928 40.0 36.0 41.0 33.0 41.0 24 37.804094602368465 40.0 36.0 41.0 33.0 41.0 25 37.67619997481083 40.0 36.0 41.0 33.0 41.0 26 37.53254849767 40.0 36.0 41.0 33.0 41.0 27 37.50576287456132 40.0 36.0 41.0 33.0 41.0 28 37.417883292429295 40.0 36.0 41.0 32.0 41.0 29 37.40323442611232 40.0 36.0 41.0 32.0 41.0 30 37.286108170483054 39.0 36.0 41.0 32.0 41.0 31 37.159498531198835 39.0 35.0 41.0 32.0 41.0 32 37.0517807724907 39.0 35.0 41.0 31.0 41.0 33 36.909548739349916 39.0 35.0 41.0 31.0 41.0 34 36.749098806237384 39.0 35.0 41.0 30.0 41.0 35 36.668833843357376 39.0 35.0 41.0 30.0 41.0 36 36.57784986571447 39.0 35.0 41.0 30.0 41.0 37 36.49823845978412 39.0 35.0 41.0 30.0 41.0 38 36.368105740068145 39.0 35.0 40.0 30.0 41.0 39 36.38686486688883 39.0 35.0 40.0 30.0 41.0 40 36.262336738410426 39.0 35.0 40.0 30.0 41.0 41 36.19271079764583 39.0 35.0 40.0 30.0 41.0 42 36.121001644104204 39.0 35.0 40.0 29.0 41.0 43 35.962056253552866 39.0 35.0 40.0 29.0 41.0 44 35.74717898269776 38.0 35.0 40.0 28.0 41.0 45 35.650803160220164 38.0 35.0 40.0 28.0 41.0 46 35.56467320450546 38.0 35.0 40.0 27.0 41.0 47 35.433893735724716 38.0 34.0 40.0 27.0 41.0 48 35.35680294917574 38.0 34.0 40.0 27.0 41.0 49 35.21973299475452 38.0 34.0 40.0 26.0 41.0 50 35.031830265813866 38.0 34.0 40.0 26.0 41.0 51 33.140787059572396 36.0 31.0 39.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 4.0 8 13.0 9 33.0 10 29.0 11 25.0 12 19.0 13 19.0 14 25.0 15 42.0 16 76.0 17 154.0 18 261.0 19 444.0 20 694.0 21 1161.0 22 1712.0 23 2446.0 24 3398.0 25 4526.0 26 5703.0 27 6806.0 28 7862.0 29 9540.0 30 11691.0 31 14748.0 32 19545.0 33 27235.0 34 39582.0 35 49247.0 36 58693.0 37 92558.0 38 116465.0 39 112793.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.97680213222955 19.822348243735895 23.948266882703546 11.25258274133101 2 32.29337218366312 24.304489459692217 26.08968707557093 17.31245128107374 3 33.303662301677804 24.958897394962847 26.390595587809802 15.34684471554955 4 28.27876246268428 26.583599124506 26.158106318738362 18.979532094071352 5 22.434363479782284 39.57780901840512 21.352250176154023 16.635577325658577 6 25.31426898633998 36.66148132767371 23.850743931621604 14.173505754364705 7 70.50824264663333 19.193810271055938 6.646538020335152 3.6514090619755804 8 84.78556864560534 5.018942939712775 6.928554651997944 3.2669337626839408 9 79.06507316774288 7.11951582322646 8.85910061032688 4.956310398703779 10 38.968673517668165 31.329035288671342 16.147111584637326 13.55517960902317 11 28.573543878520102 27.92492264540791 25.599008771959685 17.902524704112302 12 26.7861337000514 25.500805032388513 28.503592861251903 19.209468406308186 13 24.46447475466085 26.656954084220345 29.968649690071043 18.909921471047767 14 21.065468025066632 30.041834452663075 28.427004156213727 20.465693366056566 15 19.827113763160494 28.047975164835915 32.90778379519159 19.217127276812004 16 23.66250591435 27.310851428124057 30.351423018139613 18.675219639386338 17 23.897548140256045 26.289328300037102 28.394496505853077 21.418627053853772 18 24.66258420502626 25.88715250002553 29.666039206609096 19.784224088339116 19 24.74819335754671 27.610908954751395 27.55610548136852 20.084792206333375 20 26.100920085643192 27.203286846825993 28.032146832461358 18.663646235069457 21 24.929283095681416 27.728855560510183 28.68298062816354 18.65888071564486 22 24.83737664963561 25.156496253961336 28.079121238218104 21.927005858184952 23 22.095501009268936 27.903818202241837 28.8870469778097 21.11363381067953 24 21.895519390558142 26.035053799310358 30.24743257641 21.821994233721494 25 22.16647320927098 28.309568141821856 28.669194661256668 20.854763987650497 26 21.933132954588004 28.896578016658896 27.77702134612308 21.39326768263002 27 22.37394350136328 28.98014480371166 28.50716700082035 20.138744694104712 28 20.651548623615874 28.985591111625485 29.38385237782399 20.979007886934646 29 21.871181202068236 28.43772657491907 28.749868097230213 20.941224125782483 30 21.93381374307723 27.463688443955792 29.532434465598058 21.070063347368922 31 22.84572992439844 28.50716700082035 27.2155410396321 21.43156203514911 32 22.98273860785562 29.298243225303544 27.78672258209458 19.932295584746253 33 22.90921345101897 28.414920160529928 27.123634593586292 21.552231794864813 34 21.240771061042903 28.59175497060696 28.650983569169814 21.51649039918033 35 21.118399330104126 29.245141723143746 27.70536835763181 21.93109058912032 36 22.4975066121582 30.396014664184058 26.98968945833064 20.1167892653271 37 22.571372163239463 29.618213815240814 27.339444544671636 20.470969476848087 38 21.889051899910474 29.961841805178757 26.674143993573356 21.474962301337406 39 22.7121251833874 27.555254495756987 27.14865357056543 22.583966750290184 40 23.222035761819342 27.057598110131153 28.524356910173367 21.196009217876146 41 20.902419181896473 28.39721965980999 27.869778777780425 22.83058238051311 42 22.863941016485292 27.977683753323102 27.9342834871348 21.224091743056807 43 22.99890733447479 27.427436456904385 27.281917917331853 22.291738291288972 44 22.390452622227063 27.73191910871171 28.003383518791463 21.87424475026976 45 22.42653441215616 27.30983024539021 27.372292589276903 22.89134275317673 46 22.30671563805199 28.362499446859353 27.23409252596357 22.096692389125085 47 21.22528312291296 28.927383695796472 28.391773351896166 21.455559829394407 48 22.073715777613632 28.161156251170105 27.949090636775516 21.81603733444075 49 22.24374270279838 27.312553399347124 28.871218645435142 21.57248525241935 50 20.575810904189233 28.10720376339877 28.65864243967363 22.65834289273837 51 21.317529963203384 27.119209468406307 27.062874220922673 24.500386347467636 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 451.0 1 873.5 2 1296.0 3 2435.5 4 3575.0 5 2760.0 6 1945.0 7 1584.5 8 1224.0 9 1291.5 10 1359.0 11 1444.0 12 1529.0 13 1439.0 14 1349.0 15 1358.5 16 1368.0 17 1390.5 18 1413.0 19 1550.0 20 1687.0 21 2062.5 22 2438.0 23 2647.5 24 2857.0 25 3715.0 26 5577.5 27 6582.0 28 7733.0 29 8884.0 30 10630.5 31 12377.0 32 14284.0 33 16191.0 34 17808.5 35 19426.0 36 21245.5 37 23065.0 38 25744.5 39 28424.0 40 30778.5 41 33133.0 42 36671.5 43 40210.0 44 44638.0 45 49066.0 46 49618.0 47 50170.0 48 49134.5 49 48099.0 50 45547.0 51 42995.0 52 39918.0 53 36841.0 54 33693.0 55 30545.0 56 28319.0 57 26093.0 58 24256.0 59 22419.0 60 21043.0 61 19667.0 62 17880.0 63 16093.0 64 13483.0 65 10873.0 66 9078.0 67 7283.0 68 5896.0 69 4509.0 70 3549.5 71 2590.0 72 2323.0 73 2056.0 74 1675.0 75 998.5 76 703.0 77 522.5 78 342.0 79 265.0 80 188.0 81 179.0 82 170.0 83 131.0 84 92.0 85 69.5 86 47.0 87 40.0 88 33.0 89 24.5 90 16.0 91 10.0 92 4.0 93 4.5 94 5.0 95 5.0 96 5.0 97 2.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 587554.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.40334821237287 #Duplication Level Percentage of deduplicated Percentage of total 1 72.59923506542442 27.154544691039806 2 8.730212192084673 6.530783331768922 3 4.155641718590797 4.663047427389457 4 2.705378879677945 4.047609131319736 5 1.8977432123645825 3.549097509486978 6 1.4036251973792921 3.150016921034296 7 1.033753051803457 2.7066077753545486 8 0.788621045727219 2.3597654064752644 9 0.6380078338358853 2.1477266254066816 >10 5.982775718313955 40.98120551913112 >50 0.04748671442812098 1.162092863513125 >100 0.015675226120204017 0.9994791862794646 >500 0.0013831081870768248 0.3022924454016393 >1k 4.6103606235894164E-4 0.24573116639893666 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1425 0.24253089928755484 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG 613 0.10433083597422535 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC 588 0.10007590791654894 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06331332949822484 0.0 2 0.0 0.0 0.0 0.30941836835422787 0.0 3 0.0 0.0 0.0 0.41834452663074373 0.0 4 0.0 0.0 0.0 0.5820741582901316 0.0 5 0.0 0.0 0.0 1.120918247514271 0.0 6 0.0 0.0 0.0 1.4803745698267734 0.0 7 0.0 0.0 0.0 1.8103867899801551 0.0 8 0.0 0.0 0.0 2.3800365583418714 0.0 9 0.0 0.0 0.0 2.62409923173019 0.0 10 0.0 0.0 0.0 3.29297392239692 0.0 11 0.0 0.0 0.0 3.9795491137835843 0.0 12 0.0 0.0 0.0 4.64519005912648 0.0 13 0.0 0.0 0.0 4.895550026040159 0.0 14 0.0 0.0 0.0 5.000561650503613 0.0 15 0.0 0.0 0.0 5.205478985761308 0.0 16 0.0 0.0 0.0 5.614803064909778 0.0 17 0.0 0.0 0.0 6.110757479312539 0.0 18 0.0 0.0 0.0 6.645346640479003 0.0 19 0.0 0.0 0.0 6.971784721063936 0.0 20 0.0 0.0 0.0 7.297712210281949 0.0 21 0.0 0.0 0.0 7.689505985832792 0.0 22 0.0 0.0 0.0 8.116019974334273 0.0 23 0.0 0.0 0.0 8.546448496648818 0.0 24 0.0 0.0 0.0 8.878673279392192 0.0 25 0.0 0.0 0.0 9.198133277962537 0.0 26 0.0 0.0 0.0 9.493425285165278 0.0 27 0.0 0.0 0.0 9.773739945605 0.0 28 0.0 0.0 0.0 10.086562256405369 0.0 29 0.0 0.0 0.0 10.403639495263414 0.0 30 0.0 0.0 0.0 10.7940716938358 0.0 31 0.0 0.0 0.0 11.157272352839058 0.0 32 0.0 0.0 0.0 11.495283837740871 0.0 33 0.0 0.0 0.0 11.829040394585009 0.0 34 0.0 0.0 0.0 12.162626754306839 0.0 35 0.0 0.0 0.0 12.533996875180835 0.0 36 0.0 0.0 0.0 12.882390384543378 0.0 37 0.0 0.0 0.0 13.236910990308976 0.0 38 0.0 0.0 0.0 13.597558692477628 0.0 39 0.0 0.0 0.0 14.025434257957567 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGATG 25 3.888469E-5 45.000004 1 CGCGATA 20 7.030468E-4 45.0 31 TAACCGG 20 7.030468E-4 45.0 2 GTCCCGT 20 7.030468E-4 45.0 27 TGTAACG 20 7.030468E-4 45.0 1 CACCCGC 20 7.030468E-4 45.0 27 ACGGGTA 75 0.0 42.000004 5 CGTTTTA 55 6.002665E-11 40.909092 1 ACCGATC 50 1.0804797E-9 40.500004 10 CGACGGT 45 1.9255822E-8 40.000004 28 TACGGGT 45 1.9255822E-8 40.000004 4 CGTTATT 130 0.0 39.80769 1 GTTTACG 40 3.45437E-7 39.375 1 AGTACGG 80 0.0 39.375 2 AACGGGC 115 0.0 39.130432 4 TAACGGG 220 0.0 38.863636 3 CGATTGT 35 6.243077E-6 38.571426 10 GTAACGG 100 0.0 38.250004 2 TATGCGG 65 9.094947E-12 38.07692 2 ACGGGCT 130 0.0 38.07692 5 >>END_MODULE